scholarly journals West Nile Virus Detection in Urine

2005 ◽  
Vol 11 (8) ◽  
pp. 1294-1296 ◽  
Author(s):  
Jessica H. Tonry ◽  
Craig B. Brown ◽  
Cecil B. Cropp ◽  
Juliene K.G. Co ◽  
Shannon N. Bennett ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Virus Detection ◽  
West Nile

scholarly journals First record of West Nile Virus detection inside wild mosquitoes in Khartoum capital of Sudan using PCR

2020 ◽  
Vol 27 (12) ◽  
pp. 3359-3364
Author(s):  
Rania Ali El Hadi Mohamed ◽  
Deena M. Abdelgadir ◽  
Hind M. Bashab ◽  
Laila A. Al-Shuraym ◽  
Fadilah Sfouq Aleanizy ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Virus Detection ◽  
First Record ◽  
West Nile

Establishment and evaluation of real-time PCR for West Nile virus detection

2009 ◽  
Vol 6 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Shi Li-Jun ◽  
Lu Mao-Min ◽  
Li Gang ◽  
Li Cheng-Yao ◽  
Zhang Jin-Gang
Keyword(s):  
West Nile Virus ◽  
Real Time ◽  
Virus Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
West Nile ◽  
Capsid Protein Gene ◽  
Specific Test ◽  
Intercalating Dye ◽  
Polymerase Chain

AbstractA rapid real-time polymerase chain reaction (RT-PCR) for detecting West Nile virus (WNV) was established. Primers were designed according to the sequence of the capsid protein gene of WNV by Primer Premier 5.0. In this way, an inexpensive assay using the intercalating dye SYBR Green I was developed and validated. The amplifying curve showed that this method could successfully amplify 102 copies/μl of the WNV gene, while reference to Japanese encephalitis virus (JEV) and blank control were all negative. Tenfold successive dilutions of positive WNV DNA were used to measure the sensitivity of RT-PCR. The assay system showed high reproducibility with coefficient of variation (CV) <2%. Thus the newly established RT-PCR assay was shown to be a rapid, sensitive and specific test for detecting WNV.

scholarly journals West Nile Virus Detection and Commercial Assays

2005 ◽  
Vol 11 (7) ◽  
pp. 1154-1155 ◽  
Author(s):  
Peter A.G. Tilley ◽  
Gail A. Zachary ◽  
Roberta Walle ◽  
Paul F. Schnee
Keyword(s):  
West Nile Virus ◽  
Virus Detection ◽  
West Nile

scholarly journals Virus Detection Protocols for West Nile Virus in Vertebrate and Mosquito Specimens

2003 ◽  
Vol 41 (8) ◽  
pp. 3661-3667 ◽  
Author(s):  
E. B. Kauffman ◽  
S. A. Jones ◽  
A. P. Dupuis ◽  
K. A. Ngo ◽  
K. A. Bernard ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Virus Detection ◽  
West Nile

scholarly journals Development of a Double Antibody Sandwich ELISA for West Nile Virus Detection Using Monoclonal Antibodies against Non-Structural Protein 1

PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e108623 ◽  
Author(s):  
Xi-Xia Ding ◽  
Xiao-Feng Li ◽  
Yong-Qiang Deng ◽  
Yong-Hui Guo ◽  
Wei Hao ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
West Nile Virus ◽  
Sandwich Elisa ◽  
Virus Detection ◽  
Structural Protein ◽  
West Nile ◽  
Double Antibody

scholarly journals WEST NILE VIRUS DETECTION PROGRAMME, SURVEILLANCE OF Culex sp. IN MONTENEGRO, 2019

2019 ◽  
Vol 65 (4) ◽  
Author(s):  
Igor PAJOVIC ◽  
Miladin RALEVIC ◽  
Bojan ADZIC ◽  
Ljiljana PAJOVIC
Keyword(s):  
West Nile Virus ◽  
Virus Detection ◽  
West Nile ◽  
Culex Sp ◽  
Detection Programme

scholarly journals First serological evidence of West Nile virus among equines and birds in Kosovo, 2018-2019

2020 ◽  
Author(s):  
Agim Rexhepi ◽  
Kurtesh Sherifi ◽  
Kristaq Berxholi ◽  
Betim Xhekaj ◽  
Nesade Muja-Bajraktari ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Neutralization Test ◽  
Virus Detection ◽  
Control Program ◽  
Serum Samples ◽  
West Nile ◽  
Health Authorities ◽  
Domestic Bird ◽  
Domestic Birds ◽  
Public Health Authorities

Abstract Background: This study was conducted to survey the presence of the West Nile virus (WNV) in Kosovo by serological testing of the healthy autochthonous equine population and virus detection in birds and mosquitoes. Between January 2018 and June 2019, 260 equine serum samples, 626 mosquitoes (60 pools), 50 domestic birds and 51 wild birds were collected from different regions of Kosovo. Equine and domestic bird serum samples were tested by IgG ELISA while mosquitoes and bird viscera were tested for WNV RNA by RT-PCR. Positive ELISA samples were confirmed by Plaque Reduction Neutralization Test (PRNT) and eight by Virus Neutralization Test (VNT). Results: This is the first report providing evidence of WNV antibodies among animals in Kosovo. WNV antibodies were present in 27 out of 260 equine sera (10.38%) and one out of 50 samples in domestic birds by ELISA and PRNT. Eight of 27 positive equine serum samples were confirmed by VNT. No WNV RNA was detected in birds or mosquitoes.Conclusions: The occurrence of WNV antibodies in autochthonous equines from all regions of Kosovo indicates that the virus is circulating within the country. Public health authorities should therefore plan a risk assessment and disease control program.

scholarly journals Rapid assessment of West Nile virus circulation in a German zoo based on honey-baited FTA cards in combination with box gravid traps

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Noelle Fynmore ◽  
Renke Lühken ◽  
Heike Maisch ◽  
Tina Risch ◽  
Sabine Merz ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Rapid Assessment ◽  
Gravid Female ◽  
Virus Detection ◽  
Turnaround Time ◽  
Viral Rna ◽  
Mass Trapping ◽  
Vector Surveillance ◽  
West Nile ◽  
Fta Cards

Abstract Background For over a decade, monitoring of West Nile virus (WNV) in Germany has consisted of a bird monitoring programme as well as a mosquito-based surveillance programme employing CO2-baited encephalitis vector surveillance (EVS) traps for mass trapping and screening of mosquitoes. In contrast to the EVS traps, the Reiter/Cummings type box gravid trap collects gravid female mosquitoes, which have already taken a blood meal, increasing the likelihood of being infected with pathogens. The traps can be equipped with a honey-baited Flinders Technology Associates® (FTA) card to encourage sugar feeding by the trapped mosquitoes. FTA cards contain nucleic acid preserving substances, which prevent the degradation of viral RNA in the expectorated mosquito saliva and allows for testing the card for flavivirus RNA. This study aimed to assess the suitability of the method for WNV surveillance in Germany as an alternative to previous methods, which are expensive, time-consuming, and predominantly target host-seeking populations less likely to be infected with WNV. Methods In the Thüringer Zoopark Erfurt, snowy owls (Nyctea scandiaca) and greater flamingos (Phoenicopterus roseus) died of WNV infections in July and August 2020. In response, five Reiter/Cummings type box gravid traps were positioned during the daytime on the 10th, 13th, and 16th of September in five different locations. The FTA cards and mosquitoes in the chamber were collected, kept in a cool chain, and further processed for virus detection using a modified generic flavivirus reverse transcription PCR. Results A total of 15 trappings during September collected a total of 259 female mosquitoes, 97% of which were Culex pipiens sensu lato, as well as 14 honey-baited FTA cards. Eight mosquitoes tested PCR-positive for WNV. Four FTA cards tested PCR-positive for mosquito-borne flaviviruses, two of which were confirmed as WNV, and the remaining two confirmed as Usutu virus. Conclusion The suitability of the FTA cards in preserving viral RNA in the field and rapid turnaround time from collection to result is combined with a simple, cost-effective, and highly specific trapping method to create an arbovirus surveillance system, which circumvents many of the difficulties of previous surveillance programmes that required the analysis of mosquitoes in the laboratory. Graphical Abstract

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