scholarly journals Effects of hepatitis C virus NS3 protein on expression of heat shock protein 70 and Glypican3 as the markers of hepatocellular carcinoma

2021 ◽  
pp. 356-364
Keyword(s):  
Cell Proliferation ◽  
Hepatitis C ◽  
Cell Signaling ◽  
Signaling Pathways ◽  
Heat Shock Protein 70 ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Ns3 Protein ◽  
Cell Signaling Pathways

Background and Aims: Hepatitis C virus (HCV) infection is an important risk factor for the development of liver cancer. The HCV NS3 protein plays a key role in the virus life cycle and can affect normal cellular activities, such as cell proliferation, cell death, and cell signaling pathways. Moreover, it may influence malignancy development. Two cellular genes, heat shock protein 70 (HSP70) and Glypican3 (GPC3), that are assessed in this study, play important roles in the regulation of the cell signaling pathways, including cell proliferation. This study aimed to evaluate the effects of HCV NS3 protein on the expressions of these two genes in the Balb/C mouse model. Materials and Methods: This study was performed on three groups of male mice of Balb/C (n=8). The first group received NS3 plasmid, the second group received hepatitis B virus HBx plasmid, and the negative control group received distilled water. Two injections were administered via the tail vein, and after the last injection, RNA was extracted from the liver tissue. Next, the cDNA synthesis and real-time polymerase chain reaction for relevant genes were performed. Results: Findings revealed that the relative expression of the selected genes in the NS3 group was significant in comparison with the negative control group (P=0.0229 for GPC3 and 0.0020 for HSP70). However, there was no significant difference between the NS3 group and the HBx group (P=0.4516 for GPC3 and 0.6740 for HSP70). Conclusion: Results showed that NS3 protein may affect the increasing expression of the mentioned genes. Nevertheless, for more precise understanding, much more studies should be performed, such as evaluation of the effect of NS3 on other involved proteins in cell signaling pathways, studying other domains of NS3, performance of pathological and histological tests, usage of various experimental methods, assessment of the role of NS4A as a cofactor for NS3, and usage of vectors with more stability.

Biological Effects of Gyrophoric Acid and Other Lichen Derived Metabolites, on Cell Proliferation, Apoptosis and Cell Signaling pathways

2022 ◽  
Vol 351 ◽  
pp. 109768
Author(s):  
Mahshid Mohammadi ◽  
Leila Bagheri ◽  
Amr Badreldin ◽  
Pedram Fatehi ◽  
Leila Pakzad ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Signaling ◽  
Signaling Pathways ◽  
Biological Effects ◽  
Cell Signaling Pathways ◽  
Gyrophoric Acid

scholarly journals Assessment of Anti-Ageing Potential of Consciousness Energy Healing Treated DMEM and HFF-1 Cells using Cellular Proliferation and Collagen Metabolism Assays

2019 ◽  
Vol 4 (1) ◽  
pp. 1-6
Author(s):  
Mahendra Kumar Trivedi ◽  
Dahryn Trivedi ◽  
Alice Branton ◽  
Gopal Nayak ◽  
Sambhu Charan Mondal ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Line ◽  
Cellular Proliferation ◽  
Negative Control Group ◽  
Skin Aging ◽  
Negative Control ◽  
Control Group ◽  
Complex Biological Process ◽  
Collagen Level ◽  
Energy Healing

Skin health and aging are the complex biological process influenced by several intrinsic (or endogenous) and extrinsic (or exogenous) factors. Various skin-based therapies are currently available to rejuvenate the skin, but they might be related with some side-effects such as scarring. The objective of the present study was to evaluate the effect of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the human foreskin fibroblast (HFF-1) cell line and Dulbecco’s Modified Eagle Medium (DMEM) for skin health parameters like cell proliferation and synthesis of collagen. The rate of cellular proliferation in HFF-1 cells was identified, and the results found that the Biofield Energy Treated DMEM significantly (p ≤ 0.001) increased by 152.38% compared to the negative control group. Additionally, the cell proliferation was also significantly increased by 71.43% in the Biofield Energy Treated cells compared to the negative control group. Similarly, the collagen level was significantly (p ≤ 0.001) increased by 60.42% in the Biofield Energy Treated DMEM compared with the negative control group. Hence, the results exhibited a significant improvement of collagen synthesis and cellular proliferation in the Biofield Energy Treated DMEM for improving skin health. It can be concluded that The Trivedi Effect® - Consciousness Energy Healing Treatment might be a complementary and alternative approach with respect to the skin health, anti-aging in DMEM compared with the HFF-1 cell line. Therefore, the Biofield Energy Treated DMEM could be useful for the development of effective cosmetic products for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles, etc.

PSIX-13 Activation of cell signaling pathways by galectins in cow blood

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 267-267
Author(s):  
Djaafar M Rehrah ◽  
Mulumebet Worku ◽  
Hamid Ismail
Keyword(s):  
Cell Signaling ◽  
Signaling Pathways ◽  
Plasma Protein ◽  
Group Treatment ◽  
Control Group ◽  
Antibody Array ◽  
Total Plasma ◽  
Total Plasma Protein ◽  
Significant Difference ◽  
Cell Signaling Pathways

Abstract Galectins are part of a conserved family of β-galactoside-binding proteins that contribute to critical biological events during mammalian gestation and increasingly recognized for a possible role in the immune response of the cow. The objective of this study was to evaluate the effect of Galectins on signal transduction and cell activation in cow blood. Whole blood was collected aseptically from the jugular vein of healthy Holstein Friesian dairy cows (N=3). Blood samples (2.5ml) in duplicate were treated with 150µl of the four different type of recombinant galectins (1, 3, 4, and 9) respectively and untreated samples were served as control. The concentration of total plasma protein was determined using the Pierce BCA kit. Protein expression profiling was performed using1,358 antibodies on the Full Moon BioSystems’ Signaling Explorer antibody array covering 20 cell signaling pathways, as recommended by the manufacturer using an Agilent microarray scanner. Data normalization was performed using GeneSpring GX software to generate fold changes in gene expression and then filtered to obtain a list of significantly upregulated and downregulated genes. Features were extracted from protein array images of samples treated with Galectin 1, 3, 4, 9, and untreated sample as a control group. Treatment with all four Galectins increased the concentration of total plasma protein. Average increases due to treatment with Gal1, Gal3, Gal4, and Gal9 were 27%, 10%, 20%, and 14% respectively. ANOVA test showed significance difference among the groups (p < 0.05). Dunnett option was used to compare each of the treated samples to the control group as a baseline. The results also showed that there was significant difference between the control group and any of the treated group (p < 0.05). Distinct signaling pathways are activated in response to Galectin exposure. Further studies are needed to define their regulation and functional impact on cow health.

scholarly journals Suitability of a Cellulose-Based Nanomaterial for the Remediation of Heavy Metal Contaminated Freshwaters: A Case-Study Showing the Recovery of Cadmium Induced DNA Integrity Loss, Cell Proliferation Increase, Nuclear Morphology and Chromosomal Alterations on Dreissena polymorpha

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1837 ◽  
Author(s):  
Patrizia Guidi ◽  
Margherita Bernardeschi ◽  
Mara Palumbo ◽  
Massimo Genovese ◽  
Vittoria Scarcelli ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Dreissena Polymorpha ◽  
Physiological Activity ◽  
Control Level ◽  
Negative Control Group ◽  
Negative Control ◽  
Cellular Damage ◽  
Control Group ◽  
Dna Integrity ◽  
Remediation Process

The contamination of freshwaters by heavy metals represents a great problem, posing a threat for human and environmental health. Cadmium is classified as carcinogen to humans and its mechanism of carcinogenicity includes genotoxic events. In this study a recently developed eco-friendly cellulose-based nanosponge (CNS) was investigated as a candidate in freshwater nano-remediation process. For this purpose, CdCl2 (0.05 mg L−1) contaminated artificial freshwater (AFW) was treated with CNS (1.25 g L−1 for 2 h), and cellular responses were analyzed before and after CNS treatment in Dreissena polymorpha hemocytes. A control group (AFW) and a negative control group (CNS in AFW) were also tested. DNA primary damage was evaluated by Comet assay while chromosomal damage and cell proliferation were assessed by Cytome assay. AFW exposed to CNS did not cause any genotoxic effect in zebra mussel hemocytes. Moreover, DNA damage and cell proliferation induced by Cd(II) turned down to control level after 2 days when CNS were used. A reduction of Cd(II)-induced micronuclei and nuclear abnormalities was also observed. CNS was thus found to be a safe and effective candidate in cadmium remediation process being efficient in metal sequestering, restoring cellular damage exerted by Cd(II) exposure, without altering cellular physiological activity.

Therapeutic Potential of Polyphenols and their Nanoformulations in the Treatment of Colorectal Cancer

2020 ◽  
Vol 21 ◽  
Author(s):  
Shaheer H. Khan ◽  
Fahad A. Alhumaydhi ◽  
Masood A. Khan ◽  
Hina Younus
Keyword(s):  
Colorectal Cancer ◽  
Cell Proliferation ◽  
Cell Signaling ◽  
Signaling Pathways ◽  
Cancer Cell ◽  
Therapeutic Potential ◽  
Aqueous Solubility ◽  
Therapeutic Benefits ◽  
Test Models ◽  
Cell Signaling Pathways

Background: Colorectal cancer (CRC) ranks third among all cancer-related deaths around the globe. Chemotherapy may prolong the survival of CRC patients to some extent, but its clinical use is associated with grave side effects on overall health. Contrary to chemotherapy, the use of plant-derived therapeutic molecules offered advantages because of their reduced toxicity. Polyphenol is group of phytochemicals that impart many therapeutic benefits in the treatment of diabetes, cardiovascular disease and cancer. Various signaling pathways, including Wnt/β-catenin, MAPK/PI3K and TGF-β/Smad play very important roles in the development and progression of CRC. Polyphenols inhibit CRC progressions by modulating these signaling pathways e. g. curcumin and resveratrol impede cancer cell proliferation by inhibiting Wnt signaling. Because of their lower aqueous solubility, the therapeutic efficacy of polyphenols is not fully exploited. In order to increase their bioavailability and efficacy, the nanoformulations of polyphenols have been formulated and investigated against various CRC test models. The main objective of this review is to explore the potential roles of polyphenols and their nanoformulations in the treatment of colorectal cancer. Methods: We used PubMed, Web of Science, ScienceDirect, Google scholar and Scopus electronic databases by searching the keywords: Nanoparticles, Polyphenols, Colorectal cancer, Cell signaling pathways. Mostly, the articles were retrieved directly from the journals licensed to the library of Qassim University, Saudi Arabia. Results: Literature analysis has shown that the polyphenols contains several important bioactive compounds, which showed potential effectiveness against CRC. Incorporating polyphenols into nanoparticles further enhanced their bioavailability and efficacy. The findings from various studies demonstrated that polyphenol-nanoformulations accelerated the apoptosis in CRC cells by upregulating the levels of caspases and Bax, whereas inhibiting the CRC cell proliferation by dowregulating the expression of Bcl-2 and ERK1/2. Conclusions: This review provides a valuable resource on the important anti-CRC role of polyphenols and their nanoformulations. This review will expand our knowledge about the anti-CRC roles of polyphenols and their mechanisms of action through the multiple cell signaling pathways.

scholarly journals Up-Regulating ERIC by CRISPR-dCas9-VPR Inhibits Cell Proliferation and Invasion and Promotes Apoptosis in Human Bladder Cancer

2021 ◽  
Vol 8 ◽  
Author(s):  
Jiangeng Yang ◽  
An Xia ◽  
Huajie Zhang ◽  
Qi Liu ◽  
Hongke You ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Bladder Cancer ◽  
Urothelial Carcinoma ◽  
Cell Lines ◽  
Expression Patterns ◽  
Regulation Of Gene Expression ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Bladder Urothelial Carcinoma

LncRNAs are defined as non-coding RNAs that are longer than 200 nucleotides in length. The previous studys has shown that lncRNAs played important roles in the regulation of gene expression and were essential in mammalian development and disease processes. Inspired by the observation that lncRNAs are aberrantly expressed in tumors, we extracted RNA from Bladder urothelial carcinoma and matched histologically normal urothelium from each patient and bladder carcinoma cell lines. Then, we reversed transcribed them into cDNA.Last, we investigated the expression patterns of ERIC by the fluorescence quantitative PCR in bladder cancer tissues and cell lines. CRISPR-dCas9-VPR targeting ERIC plasmid was transfected into T24 and 5637 cells, and cells were classified into two groups: negative control (NC) and ERIC overexpression group. MTT assay, transwell assay, and flow cytometry were performed to examine changes in cell proliferation, invasiveness, and apoptosis. We found that the expression of ERIC was down-regulated in bladder urothelial carcinoma compared to matched histologically normal urotheliam. The differences of the expression of this gene were large in the bladder cancer lines. Compared with the negative control group, the ERIC overexpression group showed significantly decreased cell proliferation rate (t = 7.583, p = 0.002; t = 3.283, p = 0.03) and invasiveness (t = 11.538, p < 0.001; t = 8.205, p = 0.01); and increased apoptotic rate (t = −34.083, p < 0.001; t = −14.316, p < 0.001). Our study lays a foundation for further study of its pathogenic mechanism in bladder cancer.

scholarly journals Seronegative Occult Hepatitis C Virus Infection (OCI) in a Main Haemodialysis Centre In Pahang, Malaysia

2020 ◽  
Vol 18 (3) ◽  
Author(s):  
Abdul Rahman SNF ◽  
Hamzah HA ◽  
Mustafa MIA
Keyword(s):  
Hepatitis C ◽  
Viral Replication ◽  
Liver Enzymes ◽  
Negative Control Group ◽  
Negative Control ◽  
Viral Rna ◽  
Control Group ◽  
Hcv Rna ◽  
Elevated Liver Enzymes ◽  
Active Viral Replication

Introduction: Occult HCV infection has a predilection for specific populations such as haemodialysis (HD) patients. The exact natural course, epidemiology, pathogenesis and clinical importance of OCI are unknown. We investigated the existence of OCI among local patients undergoing routine HD at a referral hospital in Pahang, Malaysia. Methods: Serum and peripheral blood mononuclear cells (PMBCs) were collected from peripheral venous blood samples of seropositive (anti-HCV positive) and seronegative (anti-HCV negative) HD patients as well as healthy individuals (negative control group). Inclusion criteria for the seronegative patients included elevated liver enzymes. Both conventional PCR and strand-specific PCR were used to detect the viral RNA and to indicate active viral replication in PBMCs respectively. Direct DNA sequencing was done to confirm the viral HCV RNA and their genotypes. Results: In the majority (90-100%) of seropositive chronic hepatitis C patients, viral RNA was detected in both serum and PMBCs . Meanwhile, out of 22 seronegative patients, 6 (27%) showed active viral replication in PBMCs but no detectable viral RNA presence in the serum. None of the negative control group had detectable viral RNA. All seronegative patients with OCI were infected with HCV genotype 3 and two of them (2/6) had a slight elevation of their liver enzymes. Conclusion: Seronegative OCI does exist among local hemodialysis patients, with normal or persistently abnormal liver enzyme values. Further investigation is needed to study the mode of viral transmission and clinical significance of OCI in HD setting.

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