scholarly journals PENGARUH PEMBERIAN TOPIKAL EKSTRAK KOLAGEN KULIT IKAN LELE SANGKURIANG (CLARIAS GARIEPINUS VAR) TERHADAP TNF-Α DAN JUMLAH FIBROBLAST PADA LUKA BAKAR DERAJAT DUA TIKUS WISTAR

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Aisyah . ◽  
Zainatul Mufarikoh ◽  
Ary Andini
Keyword(s):  
Treatment Group ◽  
Healing Process ◽  
Clarias Gariepinus ◽  
Burn Injuries ◽  
Control Group ◽  
Level Ii ◽  
Tnf Α ◽  
Significant Difference ◽  
Group 2 ◽  
Group 1

ABSTRACTBackground: Collagen has vital role on healing process of burn injuries, mainly in connective tissue.Collagen triggers fibroblast proliferation, support to form new granulation tissue and epithelium aroundwound. Wound treatment through collagen extract of Sangkuriang catfish skin can increase healing process.It stimulates humidity on level II burn injuries and encourage re-epithelization, proliferation and cellmigration also increases growth. Methods: This is an experimental studies using 4 groups of RattusNorvegicus (Wistar strain). All of the group get a burn injuries in their back skin. Control group 1 (K1) wastreated by lidocaine for three days and control group 2 (K2) for ten days. Whereas treatment group 1 (P1) getlidocaine and collagen extract from Sangkuriang cat fish for 3 days and treatment group 2 (P2) for 10 days.Result: Number of fibroblast/field of view on treatment group showed significantly increased comparedcontrol groups on the 3rd days (P=0,046) and the 10th (P=0,004). Percentage of TNF-α on level II-burninjuries in wistar rats showed significant difference. Percentage of TNF-α lower than treatment group andshowed significant lowering level compared control groups on the10th (P=0,022). Conclusion: Increasing offiroblast number and lowering level of TNF-α significantly showed collagen extract of Sangkuriang cat fishskin accelerate wound healing process.

scholarly journals Why Mozart compositions during pregnancy should be exposed in the night. Study on apoptotic index of Rattus norvegicus offsprings’ brain neurons

2018 ◽  
Vol 25 (3) ◽  
pp. 97
Author(s):  
Eka Nasrur Maulana ◽  
Hermanto Tri Joewono ◽  
Widjiati Widjiati ◽  
Windhu Purnomo
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Apoptotic Index ◽  
Assay Method ◽  
Optimal Time ◽  
Control Group ◽  
Apoptosis Index ◽  
Significant Difference ◽  
Group 2 ◽  
Group 1

Objectives: To analyze the optimal time to expose Mozart compositions(in the night/ dark) by measuring brain neural apoptotic index of Rattus norvegicus offspringsMaterials and Methods: This study used experimental random-ized post test only control group design in pregnant Rattus norvegicus. Subjects were divided into three groups at random, ie control group, 1 (1 hour Mozart exposure in dark) and 2 (1 hour Mozart in light), each comprised 8 females. After delivery, 2 heaviest Rattus norvegicus offsprings were chosen, totally 15 neonates in each group, then they were sacrificed with decapita-tion and the brain was prepared and stained using TUNEL assay method and the index of neurons cell apoptosis was calculated using microscope in 1000x magnification. This study was conducted at the Faculty of Veterinary Medicine, University of Airlangga, after conducting ethical feasibility test.Results: There were no miscarriages, congenital malformation, preterm birth and deaths in all groups. The control group's neural apoptosis index was 55.98 ± 4.12, group one 24.87±3.45 and group two 23.82±3.89. Based on statistical test result, there was no significant difference of apoptosis index between treatment group 1 and treatment group 2, with p value 0,836 but significant difference was found between control group and treatment group 1 (p=0.002) and between control group and treatment group 2 (p=0.009). In dark group we found the lower average of apoptotic index, the lowest apoptotic index, 9 of 15 had the lowest, the heaviest head weight and 4 of 15 subjects had the heaviest head weightConclusion: There was no significant difference between the pro-vision of Mozart music in dark and light on brain neuron of Rattus norvegicus offsprings although lower average in the dark group especially if the extreme value exluded. In the dark group: we found  the lower average of apoptotic index, the lowest apoptotic index, biggest proportion of the lowest index, the heaviest head and biggest proportion of heaviest head.

scholarly journals INFLUENCE OF LOW OXYGEN CONDITION OF BONE MARROW MESENCHYMAL STEM CELL

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Nugroho Setyowardoyo
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Treatment Group ◽  
Group Treatment ◽  
Control Group ◽  
Low Oxygen ◽  
Significant Difference ◽  
Marrow Mesenchymal Stem Cells ◽  
Group 2 ◽  
Group 1

The state of low oxygen levels known as hypoxia in humans is considered a dangerous condition is apparently a normal physiological condition and required by the stem cells as they are in the body. Mesencyimal Stem Cells (MSCs) require physiologically optimal conditions of low O2 tension of 1-3% in the bone marrow. The purpose of this study was to reveal the difference between in vitro culture of MSC in normoxia condition (20% O 2 concentration) with hypoxia condition (1% O2 content) especially in terms of viability, pluripotent properties, and MSC proliferation ability of the culture it produces. This research is an explorative laboratory research invitro on Bone Marrow Mesenchymal Stem Cells (BMSCs) culture using hypoxia condition. The study design used Randomized Control Group Post-Test Only Design. This research was conducted for 2 months. There was a significant difference in mean slow proliferation based on the number of Least-like CFU-Cs between the control group, treatment group 1 and treatment group 2, the mean percentage of the number of cells expressing the OCT4 coding gene on immunofluorosense examination between the control group, 1 and the treatment group 2 and the mean percentage of cell numbers expressing the OCT4 coding gene on the immunofluorosense examination between the control group, the treatment group 1 and the treatment group 2 showing p <0.01. There was a significant difference of percentage of non-absorbing color cell number of trypsin blue (viable cells) between control group, treatment group 1, and treatment group 2 showing p value <0.05. This suggests that the precondition of culture with normoxia provides an opportunity for cells to adapt and proliferate before being conditioned in hypoxic cultures. Cultures with hypoxic conditions and preconditions of normoxia are the best culture conditions because they produce cells that are capable of maintaining pluripotency properties while still having better proliferation and viability capability compared with direct hypoxia conditions.Keywords: Hypoxia, normoxia, bone marrow, mesenchymal stem cells.

PENGARUH STRES (RENJATAN LISTRIK) TERHADAP AKTIVITAS MOTORIK MENCIT MENGGUNAKAN METODE KETAHANAN BERENANG DI UNIVERSITAS ANDALAS

2020 ◽  
Vol 9 (3) ◽  
pp. 11-17
Author(s):  
Isramilda . ◽  
Zulkarnain Edward
Keyword(s):  
Treatment Group ◽  
Motor Activity ◽  
Electric Shock ◽  
Control Group ◽  
Stress Treatment ◽  
A Value ◽  
Significant Difference ◽  
Group 2 ◽  
Swimming Endurance ◽  
Group 1

Stress can increase motor activity with the role of the hormones epinephrine, norepinephrine, and the sympathetic nervous system. This study aims to determine the effect of stress (electric shock) on the motor activity of mice using swimming endurance methods at Andalas University in 2019. This research is a pure experimental study with a research design is the Randomized Experimental Posttest Only Control Group Design. This research was conducted at the Pharmacology Laboratory of Andalas University, Padang in March 2019 to April 8, 2019. The population in this study was a group of male, healthy, mice (Mus musculus) mice aged 2-3 months, with an average weight of 30-40 grams. A sample of 30 was determined by the Federer Formula, divided into 3 groups which had been adapted for 1 week. Control group (K) without stress treatment, treatment group 1 (P1) with stress treatment (electric shock), and treatment group 2 (P2) with stress treatment (electric shock) and diazepam 0.26 ml. Analysis of the results using the statistical analysis of variance (ANOVA) test. Stress treatment (electric shock) in the treatment group caused motor activity in mice to increase. The results showed a significant difference between the control group (K) with the treatment group 1 (P1) with a value of p = 0.046, the control group (K) with the treatment group 2 (P2) with a value of p = 0,000, and the treatment group 1 (P1) with treatment group 2 (P2) with a value of p = 0.006. Then Ha is accepted and H0 is rejected. Based on the results of this study it can be concluded that there is an effect of stress (electric shock) on the motor activity of mice with the swimming endurance method, where stress can increase the motor activity of the mice as evidenced by the rapid sinking of mice on the swimming endurance method.

scholarly journals The Application of Equine Chorionic Gonadotropin (Ecg) and Prostaglandin F2α to Increase the Rate of Pregnancy in Bali Cattle at Buleleng, Bali

2017 ◽  
Vol 3 (6) ◽  
pp. 734
Author(s):  
Yugenthri A/P Chandran ◽  
Herry Agoes Hermadi ◽  
Eka Pramyrtha Hestianah
Keyword(s):  
Treatment Group ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Control Group ◽  
Estrus Cycle ◽  
Significant Difference ◽  
Bali Cattle ◽  
Group 2 ◽  
Equine Chorionic Gonadotropin ◽  
Group 1

This research objective was to increase the rate of pregnancy in Bali cattle using eCG and PGF2 14α">  at Buleleng, Bali. The experimental animal used in this study comprised of 18 female Bali cattles, with normal estrus cycle and 18 months old of age. The Bali cattles are all healthy with a body score of at least 2. The Control Group was injected with PGF2α 25 mg intramuscular twice on day 0 and day 11. Treatment Group 1 was injected with PGF2α 25 mg and eCG dosage of 400 IU intramuscular. Treatment Group 2 was injected with PGF2α 25 mg and eCG dosage of 600 IU intramuscular. On day 14, the female Bali cattle both Treatment Group 1 and 2 respectively showed signs of estrus. Few hours’ later artificial insemination was performed on the same day. On Day 30, all 18 female Bali cattle were checked for pregnancy using Ultrasonography (USG). The female Bali cattle were assumed to be pregnant because there were signs of enlargement in cornua uteri unilaterally.  Therefore, this indicates that there was no significant difference between Treatment Group 1 and Treatment Group 2 in pregnancy rate. The results from Control Group showed 4 pregnant and 2 not-pregnant; Treatment Group 1 showed 6 pregnant; Treatment Group 2 showed 6 pregnant. Based on the results, Control Group showed 88% of the Bali cattle were pregnant. Meanwhile, Treatment Group 1 and Treatment Group 2 showed 100% pregnant from the total 18 Bali cattle. Therefore, this research showed a positive feedback consistent with the objective. Key words: Bali Cattle, eCG, PGF2 14α">  and The Rate of Pregnancy.

scholarly journals Activity of TNF-α in patients with acute and chronic pancreatitis

2018 ◽  
Vol 22 (3) ◽  
pp. 529-531
Author(s):  
K.Ie. Ishcheikin ◽  
D.I. Grebeniuk ◽  
N.A. Liakhovchenko ◽  
O.M. Zatserkovna ◽  
O.M. Bilyk
Keyword(s):  
Chronic Pancreatitis ◽  
Control Group ◽  
Tnf Α ◽  
Significant Difference ◽  
Separate Control ◽  
Edematous Pancreatitis ◽  
Group 2 ◽  
Acute And Chronic Pancreatitis ◽  
Numerical Index ◽  
Group 1

The aim of the study was to study the activity of TNF-α in patients with acute and chronic pancreatitis. The study included 86 patients with a confirmed diagnosis of acute edematous pancreatitis (group 1) and 36 patients with a confirmed diagnosis of chronic pancreatitis (group 2). A separate control group consisted of 70 conditionally healthy people, in whom laboratory and instrumental indices were determined similar to those in patients with acute and chronic pancreatitis. According to the aim and objectives of the study, we analyzed the levels of amylase, lipase and TNF-α. The level of amylase in group 1 was 1664.1±185.2 U/L and was significantly (p<0.01) different from the same indicator in group 2 (81.2±40.2 U/L) and in the control group (75.2±23.2 U/L). A similar trend was observed in the study of lipase levels in all groups. Thus, the level of lipase in group 1 was 1058.5±120.7 U/L and was significantly (p<0.01) different from the same indicator in group 2 (37.6±22.1 U/L) and in the control group (42.7±13.8 U/L). At the same time, there was no significant difference between the indicators of group 2 and the control group (p>0.05). Regarding the levels of TNF- α, its highest rates were observed in group 1 - 65.2±7.8 pg/mL. In group 2, the level of this marker was 52.5±6.2 pg/mL, and in the control group - 48.9±5.6 pg/mL. In a statistical analysis, it turned out that the level of TNF-α was significantly higher (p<0.05) in group 1 than in group 2 and the control group. Although we noted an increase in the numerical index of the level of TNF-α in group 2, however, there was no significant difference between this indicator in group 2 and the control group (p>0.05). So, in acute pancreatitis, the levels of TNF-α were significantly higher (p<0.05) than in chronic pancreatitis, but its concentration did not correlate with other studied parameters.

scholarly journals Levels of TNF-α and IL-8 in patients with acute and chronic pancreatitis

2018 ◽  
Vol 22 (4) ◽  
pp. 666-668
Author(s):  
K.Ie. Ishcheikin ◽  
D.I. Grebeniuk ◽  
O.M. Zatserkovna ◽  
N.A. Liakhovchenko
Keyword(s):  
Chronic Pancreatitis ◽  
Control Group ◽  
Tnf Α ◽  
Significant Difference ◽  
Edematous Pancreatitis ◽  
Group A ◽  
Acute Form ◽  
Group 2 ◽  
Acute And Chronic Pancreatitis ◽  
Group 1

The aim of the study was to research levels of TNF-α and IL-8 in patients with acute and chronic pancreatitis. The study included 86 patients with a confirmed diagnosis of acute edematous pancreatitis (group 1) and 36 patients with a confirmed diagnosis of chronic pancreatitis (group 2). Control group consisted of 70 conditionally healthy people, in whom laboratory and instrumental indices were determined similar to those in patients with acute and chronic pancreatitis. According to the aim and objectives of the study, we analyzed the levels of amylase, lipase, TNF-α and IL-8. The data was processed using the SPSS 20.0 statistical software package for Windows. Determined that the level of amylase in group 1 was significantly (p<0.01) different from the same indicator in group 2 and in the control group. A similar trend was observed in the study of lipase levels in all groups. At the same time, there was no significant difference between the indicators of group 2 and the control group (p>0.05). Regarding the levels of TNF-α, its highest rates were observed in group 1. In a statistical analysis, it turned out that the level of TNF-α was significantly higher (p<0.05) in group 1 than in group 2 and the control group. The levels of IL-8 both in group 1 and group 2 were significantly higher (p<0.01) than in the control group. At the same time, the value of this indicator also differed significantly in the statistical comparison of groups 1 and 2. Thus, in acute pancreatitis, the levels of TNF-α were significantly higher (p<0.05) than in chronic pancreatitis, but its concentration did not correlate with other studied parameters. IL-8 levels progressively increase with an increase in the degree of activity of the inflammatory process, both in acute and in chronic pancreatitis, and correlates with an increase in such indicators as amylase and lipase in the acute form of the disease.

scholarly journals Biomechanical, histological, and radiological effects of different phosphodiesterase inhibitors on femoral fracture healing in rats

2018 ◽  
Vol 26 (2) ◽  
pp. 230949901877788 ◽  
Author(s):  
Yasar Mahsut Dincel ◽  
Ender Alagoz ◽  
Yavuz Arikan ◽  
Aysel Kara Caglar ◽  
Suzan Cansel Dogru ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Femoral Fracture ◽  
Healing Process ◽  
Biomechanical Analysis ◽  
Control Group ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Positive Effect ◽  
Group 1

Purpose: To investigate the biomechanical, histological, and radiological effects of sildenafil and pentoxifylline on femoral fracture healing in rats. Methods: Forty-eight Sprague-Dawley rats were divided into three groups equally according to the pharmacological agents to be investigated. Femoral shaft fractures were formed in the left side. Group 1 (control group), group 2, and group 3 were administered with saline, sildenafil, and pentoxifylline during the fracture healing process, respectively. Eight rats from each group were euthanized on days 15 and 30. X-ray images of the rats were taken after euthanasia for radiographical examination. Femur samples were subjected to histopathological and biomechanical (three-point bending) examinations. Results: Radiologically, no difference between the Goldberg scores of the groups was found for day 15 ( p > 0.05), while higher Goldberg scores were obtained from group 2 than that of group 1 ( p > 0.05) and group 3 ( p < 0.05) for day 30. In the biomechanical analysis, higher mean breaking forces were found both for day 15 and day 30 from group 2 than those obtained from group 1 (for day 15 p > 0.05 and day 30 p > 0.05) and group 3 (for day 15 p < 0.05 and day 30 p < 0.01). Higher mean absorbed energy values were obtained from group 2 than those obtained from group 1 (for day 15 p > 0.05 and day 30 p < 0.05) and group 3 (for day 15 p < 0.01 and day 30 p < 0.01). A significant difference was not found between the histological scores of all groups ( p > 0.05) for day 15, while the histological score of group 1 on day 30 was found to be significantly lower than that of sildenafil and pentoxifylline groups ( p < 0.05). Conclusion: Sildenafil had a positive effect on fracture healing, while pentoxifylline did not provide consistent positive effect.

scholarly journals Objective assessment of stiffness in Achilles tendinopathy: a novel approach using the MyotonPRO

2018 ◽  
Vol 4 (1) ◽  
pp. e000446 ◽  
Author(s):  
Gafin Ericson Morgan ◽  
Rhodri Martin ◽  
Lisa Williams ◽  
Owen Pearce ◽  
Keith Morris
Keyword(s):  
Dynamic Stiffness ◽  
Objective Assessment ◽  
Weight Bearing ◽  
Achilles Tendinopathy ◽  
Control Group ◽  
Asymptomatic Group ◽  
Musculotendinous Junction ◽  
Significant Difference ◽  
Group 2 ◽  
Group 1

ObjectivesThe aim of this study was to establish quantitative values for asymptomatic and symptomatic Achilles tendons.DesignCohort study with a single (cross-sectional) time point of patients diagnosed with unilateral Achilles tendinopathy and an asymptomatic group with comparative homogeneity.MethodsA sample of 50 participants: 25 diagnosed with symptomatic unilateral Achilles tendinopathy (AT group) and 25 with asymptomatic Achilles tendons (control group 2). The asymptomatic side of the AT group was used as a control (control group 1). Measurements at 2 cm intervals on the tendon from its insertion at the calcaneum up to the musculotendinous junction were taken non-weight bearing (NWB) and weight bearing (WB) using the MyotonPRO.ResultsThere was a significant (p<0.005) decrease in natural oscillation frequency (F) at points 2, 3 and 4 of the AT group (NWB condition) and points 2 and 3 for the WB condition. There was a significant (p<0.005) increase in logarithmic decrement (D) at points 2 and 3 signifying a decrease in elasticity. Dynamic stiffness (S) was significantly (p<0.005) reduced in the AT group at points 2 and 3 WB and point 3 WB. There was no significant difference in creep (C) observed between the symptomatic and asymptomatic tendons. There was a significant (p<0.005) increase in mechanical stress relaxation time (R) at point 2 NWB.There was a correlation between body weight and gender on tendon mechanics, with the symptomatic tendons. No significant differences were observed between the control group 1 and control group 2.ConclusionsThe MyotonPRO measured decreased stiffness over a section of the tendon corresponding clinically with Achilles tendinopathy. This may have potential in identifying risk of injury and informing rehabilitation, however further extensive research is required to generate baseline data for specific population groups monitoring variables over time. Age, gender and body mass index appear to have some bearing on the mechanical properties of the tendon but mainly in the tendinopathy group.

scholarly journals Effect of Varicocelectomy on the Frequency of Nocturnal Sperm Emissions

2015 ◽  
Vol 10 (3) ◽  
pp. 250-253
Author(s):  
Cengiz Kara ◽  
Ozgu Aydogdu ◽  
Ural Oguz ◽  
Mehmet Giray Sönmez
Keyword(s):  
Inguinal Hernia ◽  
Depression Score ◽  
Control Group ◽  
Hormone Levels ◽  
Significant Difference ◽  
Younger Men ◽  
Serum Hormone ◽  
Postoperative Serum ◽  
Group 2 ◽  
Group 1

The aim of this study was to investigate the frequency of nocturnal sperm emissions (NSE) in varicocele patients after varicocele surgery. A total of 127 patients, 96 varicocele (Group 1) and 31 patients with inguinal hernia (Group 2) were included in this study. Mean age, laterality of surgical procedures, spermiogram results, marital status, and postoperative serum hormone levels were noted for all patients. Two groups were compared in terms of Beck depression score (BDS) and anxiety scores (AS). The frequency of NSE and libido changes in the patients during 10 days postoperatively was evaluated. The number of the patients who had NSE and increased libido were significantly higher in the varicocelectomy group when compared with the control group. No significant difference was noted between the groups in terms of BDS, AS, and serum hormone levels. No association was reported between BDS, AS, and serum hormone levels and the presence of NSE in Group 1. The incidence of NSE was higher in younger men. Increased libido was significantly associated with NSE in Group 1. Cord dissection during surgery may be a factor on increased frequency of NSEs in varicocele patients.

scholarly journals Effects of COX 1-2 Inhibitors on Prevention of Rocuronium Injection Pain: Controlled, Randomised, Double Blind Study

2021 ◽  
Author(s):  
Mehmet Sahap ◽  
Handan Gulec ◽  
Esra ozayar ◽  
Ozlem zdemir ◽  
Merve Kacan ◽  
...  
Keyword(s):  
General Anesthesia ◽  
Control Group ◽  
Anesthesia Induction ◽  
Movement Response ◽  
Rocuronium Bromide ◽  
Cox Inhibitors ◽  
Significant Difference ◽  
Group 2 ◽  
Withdrawal Response ◽  
Group 1

Purpose : Rocuronium bromide is a painful agent while general anesthesia induction. The aim of the study is to investigate the effects of cox inhibitors as a resque agent against the rocuronium pain Methodology : Sixty patients enrolled for the general anesthesia enrolled in this study. Patients were allocated into two groups (Group 1: Dexkethoprofen group, Group 2: Control group) .Pain was evaluated by during rocuronium injection, patients were scored by a scale showed below. 0;No movement response to injection 1;Mild movement response to injection 2;Hand withdrawal response to injection 3;Arm withdrawal response to injection We also evaluated the pain with 2 questions when the patient was in the recovery room.Question 1.What was the last feeling before you fall into sleep? and question 2. Did you feel any pain on your hand during medication injection for anesthesia? Results:There were differences between the groups in terms of total pain score for example in group 1 there were 16 (53%) patients who did not make any movement during rocuronium injection while there were 22 (73%) patients in group 2.There was significant difference in injection rocuronium bromide pain between group 1: dexkethoprofen group, Group 2: control group in terms of the answer to the second question.Patients felt less pain than the control group. Conclusion:The effect of cox inhibitors on rocuronium pain was seen in our study but check is also effective in reducing pain in vascular width.

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