scholarly journals Biochemical Analysis and Physico Chemical Stability of a Partially Purified Lectin from Hilsha Eggs

1970 ◽  
Vol 17 ◽  
pp. 35-40 ◽  
Author(s):  
Imtiaj Hasan ◽  
Syed Rashel Kabir ◽  
Md Ashraful Haque ◽  
Nurul Absar
Keyword(s):  
Biochemical Analysis ◽  
Chelating Agent ◽  
Effect Of Temperature ◽  
Free Sugar ◽  
Chromatographic Technique ◽  
Specific Class ◽  
Physico Chemical ◽  
Nutritional Analysis ◽  
Calcium Phosphorus ◽  
Ph Inactivation

Context: Hilsha is one of the most popular fish in Bangladesh. Biochemical analysis of Hilsha egg revealed that it is quite a healthful food. A specific class of proteins called lectins is partially purified from this source.Objectives: To carry the nutritional analysis of Hilsha eggs and to isolate sugar-specific lectins from the animal source by applying effective purification techniques.Materials and Methods: The moisture, ash, protein, lipid, polysaccharide, free sugar, cholesterol, calcium, phosphorus and iron contents were determined by the conventional methods and the lectins were isolated by using the affinity chromatographic technique. The effect of temperature, pH and metal ions were observed by performing the hemagglutination assay.Results: The moisture, ash, protein, lipid, polysaccharide, free sugar, cholesterol, calcium, phosphorus and iron contents were 34-, 2.4-, 32.59-, 28.35-, 0.036-, 0.059-, 0.40-, 0.178-, 0.193- and 0.136%, respectively. The partially purified crude protein by affinity chromatography from Hilsha eggs agglutinated rabbit erythrocytes and the hemagglutinating activity is inhibited by 25mM Raffinose followed by 100 mM of glucose, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine and D-galactosamine-HCl each. In the presence of the chelating agent EDTA, the lectin lost its activity completely. Thermal and pH inactivation assay of the lectin indicates that the activity is highest at 30 to 40°C and at the pH 5 to 9, respectively. Conclusion: Hilsha fish eggs can be recommended as a nutritious food and as well as can be regarded as a source of animal lectins, a group of sugar-binding proteins.Key words: Nutritional analysis; Hemagglutination assay; Temperature; pH; Tenualosa ilisha.DOI: 10.3329/jbs.v17i0.7097J. bio-sci. 17: 35-40, 2009

Effect of temperature, salinity, heavy metals, ammonium concentration, pH and dissolved oxygen on ammonium removal by an aerobic nitrifier

RSC Advances ◽  
2015 ◽  
Vol 5 (97) ◽  
pp. 79988-79996 ◽  
Author(s):  
Ying Wang ◽  
Hu Chen ◽  
Yu-Xiang Liu ◽  
Rui-Peng Ren ◽  
Yong-Kang Lv
Keyword(s):  
Gene Sequence ◽  
High Salinity ◽  
Alcaligenes Faecalis ◽  
Effect Of Temperature ◽  
Ammonium Concentration ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Physico Chemical ◽  
Gene Sequence Analysis ◽  
Chemical Conditions

An aerobic nitrifier WY-01 was identified asAlcaligenes faecalisby its 16S rRNA gene sequence analysis. It could remove ammonium effectively in varying physico-chemical conditions, such as low temperature, high salinity and high ammonium loads.

The Effect of Hydrothermal Dewatering Temperature on Hydro-Char Obtained from Oily Scum

2019 ◽  
Vol 971 ◽  
pp. 127-133
Author(s):  
Shuang Hui Deng ◽  
Xue Bin Wang ◽  
Dao Yang Ma ◽  
Xu Chao Lu ◽  
Hou Zhang Tan
Keyword(s):  
Particle Size ◽  
Moisture Content ◽  
Surface Characteristics ◽  
Gas Content ◽  
Effect Of Temperature ◽  
Content Increase ◽  
Liquid Content ◽  
Physico Chemical ◽  
Methylene Groups ◽  
Reactor Temperature

Oily scum was treated by hydrothermal dewatering (HTD) under 120-240°C. The changes of surface characteristics and physico-chemical structure of oily scum were investigated and the effect of temperature on the properties of the hydro-char obtained from oily scum was analyzed through different characterization techniques. Results show that the moisture content of HTD treated hydro-char decreases as the temperature increases, which implies that the oily scum can be dewatered and upgraded through HTD method. The morphologies of the HTD pretreated oily scum present less oil content, smaller particle size, and become darker. As the reactor temperature increases from 120 to 240°C during the HTD process, the moisture content of hydro-char obtained decreases from 35.51 to 11.31%, while the liquid content and hydro-char content increase from 58.53 to 79.76% and from 2.50 to 5.29%, respectively, and the released gas content slightly varies in the range of 3.14 - 4.31%. The breaking and gathering effects of the HTD upgrading on oily scum result in a wide particle size distribution of products, which indicates that the overall structure of raw oily scum has been destroyed. With the increase of HTD temperature, the shorten vibration of methylene groups in hydro-char products is weakened.

scholarly journals Effect of Temperature and Slice Size on Avocado Pulp Drying Rate and Oil Yield

2013 ◽  
Vol 34 (2) ◽  
pp. 116-124
Author(s):  
Abraham T. Temu
Keyword(s):  
Moisture Content ◽  
Chemical Properties ◽  
Oil Yield ◽  
Effect Of Temperature ◽  
Drying Rate ◽  
Seed Oils ◽  
Slice Thickness ◽  
Physico Chemical ◽  
Thin Layer Drying ◽  
Drying Models

The effect of temperature and slice thickness on avocado pulp drying rate and oil yield was determined at 50 o C, 60 o C and 70 o C. Pulp slices of 2mm and 5 mm thick were used. The avocado pulp was found to contain 74-75% (wet basis) water, which makes extraction of oil by both chemical and mechanical means difficult. Experimental results showed that oil yield increased with decreasing moisture content reaching a yield of 61% at 10% moisture content. The rate of drying increased with temperature, but decreased with slice thickness. The drying data obtained agreed well with several thin-layer drying models. The models showed that the characteristic drying constant increased linearly with drying temperature and decreased with slice thickness. The Physico-chemical properties of the obtained oil compared favorably with those obtained from other conventional seed oils.

scholarly journals Estimation of The Physico-Chemical Parameters in Marine Environment (Yumurtalik Bight- Iskenderun Bay)

2016 ◽  
Vol 4 (2) ◽  
pp. 97
Author(s):  
Gökhan Tamer Kayaalp ◽  
Oya Işık ◽  
Benin Toklu Alıçlı
Keyword(s):  
Light Intensity ◽  
Analysis Of Variance ◽  
Chemical Properties ◽  
Effect Of Temperature ◽  
Chemical Parameters ◽  
Marine Life ◽  
Multiple Comparison Test ◽  
Physico Chemical ◽  
Iskenderun Bay ◽  
Dissolved O2

The study was carried out to estimate the temperature, light intensity, salinity, Dissolved O2 (DO), pH values and the biotic parameter chlorophyll- a in the water column related with the depth. Because, the physico-chemical parameters affect greatly both primary and secondary producers in marine life. For this purpose the physico-chemical properties were determined day and night for 40 meter depth during the eight days. The means were compared by using the analysis of variance method and Duncan’s Multiple Comparison Test. Also physico-chemical parameters were estimated by using the analysis of regression and correlation. The effect of temperature and salinity were found significant according to the result of the analysis of variance during the day. Also the similar results were found for the night. While the effect of the depth on the chloropyll-a a was significant in the night, the effect of the depth on the DO was not significant in the day and night. The correlations among the depth and the parameters were defined. It was found the negative correlation between the depth and the temperature and light intensity. Determination coefficient of the model for salinity was also found different for day time. The correlation values among the depth and the temperature, salinity and pH were found different for the night.

scholarly journals Universal microscopic spectrum of the unquenched QCD Dirac operator at finite temperature

2021 ◽  
Vol 2021 (12) ◽  
Author(s):  
G. Akemann ◽  
T. R. Würfel
Keyword(s):  
Dirac Operator ◽  
Random Matrix ◽  
Correlation Functions ◽  
Matrix Theory ◽  
Chiral Condensate ◽  
Effect Of Temperature ◽  
Specific Class ◽  
Chiral Phase ◽  
Determinantal Representation ◽  
Matrix Ensemble

Abstract In the ε-regime of chiral perturbation theory the spectral correlations of the Euclidean QCD Dirac operator close to the origin can be computed using random matrix theory. To incorporate the effect of temperature, a random matrix ensemble has been proposed, where a constant, deterministic matrix is added to the Dirac operator. Its eigenvalue correlation functions can be written as the determinant of a kernel that depends on temperature. Due to recent progress in this specific class of random matrix ensembles, featuring a deterministic, additive shift, we can determine the limiting kernel and correlation functions in this class, which is the class of polynomial ensembles. We prove the equivalence between this new determinantal representation of the microscopic eigenvalue correlation functions and existing results in terms of determinants of different sizes, for an arbitrary number of quark flavours, with and without temperature, and extend them to non-zero topology. These results all agree and are thus universal when measured in units of the temperature dependent chiral condensate, as long as we stay below the chiral phase transition.

scholarly journals Growth Performance and Nutritional Analysis of Spirulina platensis in Different Concentrations of Papaya Skin Powder Media

1970 ◽  
Vol 46 (3) ◽  
pp. 333-338 ◽  
Author(s):  
MA Toyub ◽  
MZ Uddin ◽  
MI Miah ◽  
MAB Habib
Keyword(s):  
Growth Performance ◽  
Chlorophyll A ◽  
Spirulina Platensis ◽  
Specific Growth ◽  
Cell Weight ◽  
Skin Cell ◽  
Physico Chemical ◽  
Nutritional Analysis ◽  
Chemical Factors ◽  
Chlorophyll A Content

The growth performance of Spirulina platensis was studied in four different concentrations viz. 0.3, 0.4, 0.5 and 0.6 g/l of papaya (Carica papaya) skin powder media (PSPM) and in control, Kosaric Medium (KM) in triplicate. The initial cell weight of S. platensis was 6.0 mg/l which attained a maximum weight of 913.13 mg/l on the 10th day of culture in the control KM followed by 699.07, 612.13, 538.99 and 377.48 mg/l that grown in 0.40, 0.30, 0.50 and 0.60 g/l of PSPM respectively. Similar trend was observed in the case of chlorophyll a content ranging 3.57 to 8.15 mg/l. The specific growth rate (SGR, μ/day) of S. platensis on the basis of cell weight and chlorophyll a content was recorded in the ranges of 0.41 to 0.50 and 0.41 to 0.49, respectively, for all the treatments. The SGR on the basis of cell weight and chlorophyll a was recorded significantly (p<0.05) higher in KM among all the treatments. On the other hand among the four different concentrations of PSPM, 0.40 g/l showed significantly (p<0.05) higher SGR than others. The pH and other physico-chemical factors were within suitable range of algae culture. The cultured microalga was nutritionally rich. Keywords: Spirulina; Papaya skin; Cell weight ; Chlorophyll a DOI: http://dx.doi.org/10.3329/bjsir.v46i3.9039 BJSIR 2011; 46(3): 333-338

scholarly journals Effect of Dehydration on Several Physico-Chemical Properties and the Antioxidant Activity of Leeks (Allium porrum L.)

2011 ◽  
Vol 39 (1) ◽  
pp. 144 ◽  
Author(s):  
Mehmet OZGUR ◽  
Arzu AKPINAR-BAYIZIT ◽  
Tulay OZCAN ◽  
Lutfiye YILMAZ-ERSAN
Keyword(s):  
Antioxidant Activity ◽  
Dry Matter ◽  
Chemical Properties ◽  
Effect Of Temperature ◽  
Allium Porrum ◽  
Drying Process ◽  
Hot Air Drying ◽  
Air Drying ◽  
Hot Air ◽  
Physico Chemical

This study investigated the changes in some physico-chemical properties and variations in antioxidant compounds of leeks (cv. ‘Inegol-92’) caused by the drying process. The dry matter and ash contents of the fresh leek samples were 8.06 and 0.58 g 100 g-1, respectively. The pH of the fresh leek samples was 6.02, and the titritable acidity in terms of citric acid was 0.14%. As expected, application of hot-air drying significantly increased the dry matter and ash values due to removal of water from the leek slices. The rehydration ratio of dried leeks at 45°C was 5.41, and the coefficient of rehydration was 0.47. The contents of chlorophyll a and b were higher in the dried leeks than in the fresh leeks. The dehydrated leeks showed a high total color difference (?E=12.53) mainly due to the effect of temperature on heat-sensitive compounds. As expected, both fresh and dried leek samples exhibited antioxidant activity with fresh leeks showing a higher capacity of antioxidant activity. Drying the leeks resulted in some ascorbic acid loss. Fresh leeks had much higher phenolic values (26.33 mg rutin eq 100 g DM-1) than the dehydrated samples. The antioxidant capacity of leeks was decreased by more than 50% during the drying process. Although being the most applied method of thermal dehydration, hot air drying causes the degradation of sensitive components, which results in significant losses in sensorial and physico-chemical properties of the dried products.

Sign in / Sign up

Export Citation Format

Share Document