scholarly journals Determination of individual coumarin in a dry extract of Melilotus officinalis L.

2021 ◽  
Vol 10 (4) ◽  
pp. 104-107
Author(s):  
M. V. Aroyan ◽  
Yu. E. Generalova ◽  
I. I. Terninko ◽  
I. E. Kaukhova ◽  
E. K. Novikova

Introduction. Substances of plant origin exhibit a variety of pharmacological activity in combination with a relatively low toxicity and frequency of side effects. In this regard, a promising direction is the standardization of substances of plant origin in the development of new herbal medicinal products.Aim. Qualitative and quantitative determination of individual coumarin in a dry extract of Melilotus оfficinalis L.Materials and methods. A dry extract of the Melilotus officinalis L. was used as an object of research. Determination of individual coumarin was carried out by high-performance liquid chromatography.Results and discussion. As a result of the research, coumarin was identified by high performance liquid chromatography based on comparison of retention times of the sample with retention times of a standard sample of coumarin in the sample of dry extract under research. The coumarin contents in the dry extract was 0.642 ± 0.007 %.Conclusion. The data obtained on the qualitative and quantitative content of coumarin in the dry extract of Melilotus оfficinalis L. were used in the development of the draft version specification of quality indicators.

Author(s):  
BS Dattilo ◽  
S Gallo ◽  
G Lionetti ◽  
SG Rossi

AbstractA new method is described for the qualitative and quantitative determination of both free and bound maleic hydrazide residues in tobacco leaves and cigarette filler by high performance liquid chromatography. Analyses were carried out by hydrolyzing samples of ground tobacco with 4 N hydrochloric acid for 40 minutes under reflux followed by sample chromatography, running isocratic elutions with a dilute solution of phosphoric acid. The quantitative determination of maleic hydrazide was performed by light absorption at 320 nm, by the calibration curve method. Recoveries of maleic hydrazide added to tobacco samples were greater than 90 %. The detection limit of the method, determined on ground tobacco leaves, was at least 5 ppm. The results obtained by this procedure and by the ISO standard method no. 4876 are in good accordance.


2019 ◽  
Vol 7 (2) ◽  
pp. 35-41 ◽  
Author(s):  
Shruti Mourya ◽  
Ramesh Bodla ◽  
Ravikant Taurean ◽  
Akanksha Sharma

Methylxanthines are mainly a group of phytochemicals which are derived from purine base xanthine. These xanthines are obtained from plant as a result of secondary metabolism. There are various physiological actions that have been attributed to these derivatives in neurogenerative diseases, respiratory diseases, cancer and diabetes. The aim of this study is to develop a suitable qualitative and quantitative method for these xanthine derivatives. HPLC method is suitable for simultaneous estimation of methylxanthines, based on their physicochemical properties. Theobromine, Theophylline and Caffeine that belongs to alkaloids possess their economic effects. They have various stimulant effects on cardiovascular system, gastrointestinal system, respiratory system, central nervous system etc. that results in increased motivation to work, increased energy and increased alertness. High performance liquid chromatography is used for simultaneous determination of theophylline, theobromine and caffeine from different tea leaves.


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