scholarly journals Optimization of a 96-Well Electroporation Assay for Postnatal Rat CNS Neurons Suitable for Cost–Effective Medium-Throughput Screening of Genes that Promote Neurite Outgrowth

Author(s):  
Thomas H. Hutson ◽  
William J. Buchser ◽  
John L. Bixby ◽  
Vance P. Lemmon ◽  
Lawrence D. F. Moon
1994 ◽  
Vol 176 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Youngsuk Oh ◽  
Shunsuke Sashihara ◽  
Stephen G. Waxman

1995 ◽  
Vol 130 (3) ◽  
pp. 701-710 ◽  
Author(s):  
M D Mark ◽  
Y Liu ◽  
S T Wong ◽  
T R Hinds ◽  
D R Storm

MAP kinase activity is necessary for growth factor induction of neurite outgrowth in PC12 cells. Although NGF and EGF both stimulate MAP kinase activity, EGF does not stimulate neurite extension. We report that EGF, in combination with KCl, stimulates neurite outgrowth in PC12 cells. This phenomenon was independent of intracellular Ca2+ increases and not due to enhancement of MAP kinase activity over that seen with EGF alone. However, EGF plus KCl increased intracellular cAMP, and other cAMP elevating agents acted synergistically with EGF to promote neurite outgrowth. Stimulation of neurite outgrowth by cAMP and EGF was blocked by inhibitors of transcription suggesting that synergistic regulation of transcription by the cAMP and MAP kinase pathways may stimulate neurite growth.


2018 ◽  
Vol 78 (12) ◽  
pp. 1191-1205 ◽  
Author(s):  
Zhisheng Ji ◽  
Guowei Zhang ◽  
Li Chen ◽  
Jiong Li ◽  
Yuhao Yang ◽  
...  

2011 ◽  
Vol 95 (3) ◽  
pp. 1123-1126 ◽  
Author(s):  
Maria Luisa Barcena de Arellano ◽  
Julia Arnold ◽  
Filiberto Vercellino ◽  
Vito Chiantera ◽  
Achim Schneider ◽  
...  

1994 ◽  
Vol 19 (3) ◽  
pp. 279-285 ◽  
Author(s):  
Yukio Akaneya ◽  
Mitsuo Takahashi ◽  
Hiroshi Hatanaka

1983 ◽  
Vol 4 (3) ◽  
pp. 323-326
Author(s):  
D. Ferraro ◽  
A. Cestelli ◽  
R. Barbieri ◽  
F. Vitale ◽  
G. Savettieri
Keyword(s):  

2013 ◽  
Vol 56 (12) ◽  
pp. 5071-5078 ◽  
Author(s):  
Mallinath B. Hadimani ◽  
Meena K. Purohit ◽  
Chandrashaker Vanampally ◽  
Randy Van der Ploeg ◽  
Victor Arballo ◽  
...  

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