scholarly journals Using Salivary Biomarkers for Stress Assessment in Offshore Saturation Diving: A Pilot Study

2021 ◽  
Vol 12 ◽  
Author(s):  
Roxane Monnoyer ◽  
Jacky Lautridou ◽  
Sanjoy Deb ◽  
Astrid Hjelde ◽  
Ingrid Eftedal
Keyword(s):  
Pilot Study ◽  
Immunoglobulin A ◽  
Alpha Amylase ◽  
Bottom Phase ◽  
Stress Assessment ◽  
Reactive Protein ◽  
Salivary Biomarkers ◽  
Tnf Α ◽  
Biomarker Analysis ◽  
Limited Opportunity

Health monitoring during offshore saturation diving is complicated due to restricted access to the divers, the desire to keep invasive procedures to a minimum, and limited opportunity for laboratory work onboard dive support vessels (DSV). In this pilot study, we examined whether measuring salivary biomarkrers in samples collected by the divers themselves might be a feasible approach to environmental stress assessment. Nine saturation divers were trained in the passive drool method for saliva collection and proceeded to collect samples at nine time points before, during, and after an offshore commercial saturation diving campaign. Samples collected within the hyperbaric living chambers were decompressed and stored frozen at −20°C onboard the DSV until they were shipped to land for analysis. Passive drool samples were collected without loss and assayed for a selection of salivary biomarkers: secretory immunoglobulin A (SIgA), C-reactive protein (CRP), tumor necrosis factor (TNF)-α, interleukins IL-6, IL-8, IL-1β, as well as cortisol and alpha-amylase. During the bottom phase of the hyperbaric saturation, SIgA, CRP, TNF-α, IL-8 and IL-1β increased significantly, whereas IL-6, cortisol and alpha-amylase were unchanged. All markers returned to pre-dive levels after the divers were decompressed back to surface pressure. We conclude that salivary biomarker analysis may be a feasible approach to stress assessment in offshore saturation diving. The results of our pilot test are consonant with an activation of the sympathetic nervous system related to systemic inflammation during hyperbaric and hyperoxic saturation.

scholarly journals Diagnostic Models for Screening of Periodontitis with Inflammatory Mediators and Microbial Profiles in Saliva

Diagnostics ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 820
Author(s):  
Jungwon Lee ◽  
Jun-Beom Lee ◽  
Hyun-Young Song ◽  
Min Jung Son ◽  
Ling Li ◽  
...  
Keyword(s):  
Immunoglobulin A ◽  
Stage Iv ◽  
Fusobacterium Nucleatum ◽  
Operating Characteristics ◽  
Active Matrix ◽  
Campylobacter Rectus ◽  
Reactive Protein ◽  
Salivary Biomarkers ◽  
Parvimonas Micra ◽  
Diagnostic Models

This study aims to investigate and assess salivary biomarkers and microbial profiles as a means of diagnosing periodontitis. A total of 121 subjects were included: 28 periodontally healthy subjects, 24 with Stage I periodontitis, 24 with Stage II, 23 with Stage III, and 22 with Stage IV. Salivary proteins (including active matrix metalloproteinase-8 (MMP-8), pro-MMP-8, total MMP-8, C-reactive protein, secretory immunoglobulin A) and planktonic bacteria (including Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia, Porphyromonas nigrescens, Parvimonas micra, Campylobacter rectus, Eubacterium nodatum, Eikenella corrodens, Streptococcus mutans, Staphylococcus aureus, Enterococcus faecalis, and Actinomyces viscosus) were measured from salivary samples. The performance of the diagnostic models was assessed by receiver operating characteristics (ROCs) and area under the ROC curve (AUC) analysis. The diagnostic models were constructed based on the subjects’ proteins and/or microbial profiles, resulting in two potential diagnosis models that achieved better diagnostic powers, with an AUC value > 0.750 for the diagnosis of Stages II, III, and IV periodontitis (Model PA-I; AUC: 0.796, sensitivity: 0.754, specificity: 0.712) and for the diagnosis of Stages III and IV periodontitis (Model PA-II; AUC: 0.796, sensitivity: 0.756, specificity: 0.868). This study can contribute to screening for periodontitis based on salivary biomarkers.

scholarly journals Salivary Biomarkers and Training Load During Training and Competition in Paralympic Swimmers

2018 ◽  
Vol 13 (7) ◽  
pp. 839-843 ◽  
Author(s):  
Ciara Sinnott-O’Connor ◽  
Thomas M. Comyns ◽  
Alan M. Nevill ◽  
Giles D. Warrington
Keyword(s):  
Stress Response ◽  
Stress Responses ◽  
Salivary Cortisol ◽  
Perceived Exertion ◽  
Immunoglobulin A ◽  
Training Load ◽  
Rating Of Perceived Exertion ◽  
Alpha Amylase ◽  
Salivary Biomarkers ◽  
Paralympic Games

Context: Stress responses in athletes can be attributed to training and competition, where increased physiological and psychological stress may negatively affect performance and recovery. Purpose: To examine the relationship between training load (TL) and salivary biomarkers immunoglobulin A (IgA), alpha-amylase (AA), and cortisol across a 16-wk preparation phase and 10-d competition phase in Paralympic swimmers. Methods: Four Paralympic swimmers provided biweekly saliva samples during 3 training phases—(1) normal training, (2) intensified training, and (3) taper—as well as daily saliva samples in the 10-d Paralympic competition (2016 Paralympic Games). TL was measured using session rating of perceived exertion. Results: Multilevel analysis identified a significant increase in salivary immunoglobulin A (sIgA: 94.98 [27.69] μg·mL−1), salivary alpha-amylase (sAA: 45.78 [19.07] μg·mL−1), and salivary cortisol (7.92 [2.17] nM) during intensified training concurrent with a 38.3% increase in TL. During the taper phase, a 49.5% decrease in TL from the intensified training phase resulted in a decrease in sIgA, sAA, and salivary cortisol; however, all 3 remained higher than baseline levels. A further significant increase was observed during competition in sIgA (168.69 [24.19] μg·mL−1), sAA (35.86 [16.67] μg·mL−1), and salivary cortisol (10.49 [1.89] nM) despite a continued decrease (77.8%) in TL from the taper phase. Conclusions: Results demonstrate that performance in major competition such as Paralympic games, despite a noticeable reduction in TL, induces a stress response in athletes. Because of the elevated stress response observed, modifications to individual postrace recovery protocols may be required to enable athletes to maximize performance across all 10 d of competition.

scholarly journals Assessing the feasibility of applying a combined osteopathy and psychoeducation therapy (OsteoPeCT) and its influence on levels of psychological and physiological stress in adult participants with moderate stress: A pilot study

2020 ◽  
Author(s):  
Antoine Rahier ◽  
Victoria J Taylor ◽  
Teena KJB Gamage ◽  
Alastair Treacher ◽  
Simon J O’Carroll ◽  
...  
Keyword(s):  
Pilot Study ◽  
Perceived Stress ◽  
Physiological Stress ◽  
Immunoglobulin A ◽  
Post Intervention ◽  
Adult Participants ◽  
Biomarker Analysis ◽  
Mood Scale ◽  
The Impact ◽  
Moderate Stress

Abstract Background: There is mounting evidence suggesting a relationship between stress and adverse health outcomes. Stress is a multidimensional phenomenon requiring a multimodal approach. While there is some evidence indicating a positive effect of massage therapy, there is limited research regarding the impact of related approaches such as general osteopathic techniques (GOTs). Further, research examining the feasibility and effectiveness of combining GOTs with psychoeducation in the management of stress is lacking. The present pilot study aimed to assess the feasibility of applying a therapy package consisting of GOTs and brief psychoeducation and its influence on moderate stress in a convenience sample.Methods: A pilot uncontrolled trial with mixed pragmatic and exploratory design was conducted. The therapy package comprised of ten GOTs and ten minutes of scripted psychoeducation (OsteoPeCT) was applied in two sessions over two consecutive days to 18 adult participants with moderate stress. Feedback from participants and challenges experienced by both participants and researchers were recorded. The effects of OsteoPeCT were assessed by measuring pre-and post- intervention scores of self-reported perceived stress (Perceived Stress Scale-10, PSS-10; Profile of Mood Scale, POMS) and salivary levels of physiological stress biomarkers (cortisol; secretory immunoglobulin A, sIgA and interleukin-6, IL-6).Results: All aspects related to the application of OsteoPeCT (participant recruitment, participant retention, therapy application, administration of health screen and self-reported perceived stress questionnaires, and the collection of saliva samples for biomarker analysis) were feasible. A total of 18 participants were enrolled. The timing of sessions on consecutive days was reported to be challenging. While a measurable decrease in perceived stress (PSS-10) and in mood scores (Tense, Fatigue, Depression, Anger) were noted post therapy (OsteoPeCT) application, physiological stress markers were unaffected. Diurnal variations of these biomarkers may need further consideration.Conclusion: The application of OsteoPeCT was feasible, well received with some beneficial influence on perceived stress indicating that an integration of psychoeducation and osteopathic care may confer benefits to patients. Future investigations with adapted protocols and larger sample size is warranted to assess effectiveness.Trial registration: Retrospectively registered in Australian New Zealand Clinical Trials Registry (registration number ACTRN12620000763943 ) and ICTRP .

scholarly journals Diagnostic Models for Screening of Chronic Periodontitis with Cytokines and Microbiologic Profiles in Saliva

2020 ◽  
Author(s):  
Jungwon Lee ◽  
Jun-Beom Lee ◽  
Hyun-Young Song ◽  
Min Jung Son ◽  
Ling Li ◽  
...  
Keyword(s):  
Immunoglobulin A ◽  
Stage Iv ◽  
Fusobacterium Nucleatum ◽  
Stage Ii ◽  
Stage Iii ◽  
Operating Characteristics ◽  
Active Matrix ◽  
Reactive Protein ◽  
Salivary Biomarkers ◽  
Diagnostic Models

This study was to investigate and assess salivary biomarkers as a means of diagnosing periodontitis. A total of 121 subjects were included: 28 periodontally healthy subjects, 24 with stage I, 24 with stage II, 23 with stage III, and 22 with stage IV periodontitis. Salivary proteins including active matrix metalloproteinase-8 (MMP-8), pro-MMP-8, total MMP-8, C-reactive protein, secretory immunoglobulin A and planktonic bacteria including Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia, Porphyromonas nigrescens, Parvimonas micra, Campylobacter rectus, Eubacterium nodatum, Eikenella corrodens, Streptococcus mitis, Streptococcus mutans, Staphylococcus aureus, Enterococcus faecalis, and Actinomyces viscosus were measured from salivary samples. The performance of the diagnostic models was assessed by receiver operating characteristics (ROC) and area under the ROC curve (AUC) analysis. The diagnostic models were constructed based on the subjects’ proteins and/or microbial profiles, resulting in two potential diagnosis models, which achieved better diagnostic powers with an AUC value > 0.750 for the diagnosis of stage II, III, and IV periodontitis (Model PC-I; AUC: 0.796, sensitivity: 0.754, specificity: 0.712) and for the diagnosis of stage III and IV periodontitis (Model PC-II; AUC: 0.796, sensitivity: 0.756, specificity: 0.868). This study can contribute to screening for periodontitis based on salivary biomarkers.

scholarly journals Evolution of Human Salivary Stress Markers during an Eight-Hour Exposure to a Mediterranean Holm Oak Forest. A Pilot Study

Forests ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1600
Author(s):  
Albert Bach ◽  
Jose Joaquin Ceron ◽  
Roser Maneja ◽  
Joan Llusià ◽  
Josep Penuelas ◽  
...  
Keyword(s):  
Pilot Study ◽  
Amylase Activity ◽  
Holm Oak ◽  
Alpha Amylase ◽  
Oak Forest ◽  
Salivary Biomarkers ◽  
Stress Markers ◽  
Human Stress

The current study analyses the evolution of different human stress markers during an 8 h exposure to a Mediterranean Holm oak forest. We conducted a pre-post study with thirty-one subjects in which saliva samples were collected before the exposure (baseline) and after 1, 2, 4 and 8 h. Our results show: (A) a significant decrease in cortisol saliva concentrations from the second hour until the end compared to basal time; (B) a significant increase in alpha amylase activity after the first hour of exposure compared to basal time that remained elevated during the rest of the study; (C) a significant decrease in IgA from the fourth hour of exposure compared to the basal time. These findings indicate an effect of forest exposure in salivary biomarkers of stress and provide relevant data for the scientific and healthcare community encouraging further research in the field.

Genetic Variation in TNF-α, its Relation with Inflammatory Biomarkers and Susceptibility to Rheumatoid Arthritis

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Khadiga Ahmed Ismail
Keyword(s):  
Rheumatoid Arthritis ◽  
Serum Level ◽  
Functional Disability ◽  
Serum Levels ◽  
Amplification Refractory Mutation System ◽  
Reactive Protein ◽  
Tnf Α ◽  
Mutation System ◽  
Potential Factor ◽  
Factor Α

Background: Tumor necrosis Factor-α (TNF-α) is encoded and controlled by TNF-α gene, which is involved in rheumatoid arthritis (RA) susceptibility. This research aimed to identify genetic variations of TNF-α (G308A) and to establish its association with inflammatory markers in Rheumatoid Arthritis predisposition. Methods: In the present study, fifty RA patients and fifty volunteers were involved and evaluated for the C-reactive protein, rheumatoid factor, and TNF-α were estimated by ELISA, Erythrocyte Sedimentation Rate (ESR) by Wintergreen method and for TNF-α-308 G>A polymorphism by polymerase chain reaction with amplification refractory mutation system (PCR-ARMS). Results: The CRP, RF, ESR and TNF-α were significantly elevated in RA patients relative to controls. The serum level TNF-α was also significantly elevated in female patients and in patients ≥50 years. Analysis of TNF-308 gene polymorphism revealed that GG genotypes were more prevalent in RA patients than in the healthy individuals and that GG genotype may be a potential factor to RA. The G allele was more common in RA than in the control. Elevated TNF-α serum levels were significantly associated the GG genotype and functional disability in RA patients. Conclusion: TNF-α promoter 308polymorphism GG genotype may be considered as a risk factor for RA and the TNF-α serum level was significantly related to the functional disability in the disease.

Synergism Effects of Ursolic Acid Supplementation on the Levels of Irisin, C-reactive Protein, IL-6, and TNF-α During High-intensity Resistance Training in Low Activity Men

2020 ◽  
Vol 20 (2) ◽  
pp. 138-144 ◽  
Author(s):  
Ehsan Asghari ◽  
Amir Rashidlamir ◽  
Seyyed R.A. Hosseini ◽  
Mahtab Moazzami ◽  
Saeed Samarghandian ◽  
...  
Keyword(s):  
Resistance Training ◽  
Ursolic Acid ◽  
High Intensity ◽  
Plasma Levels ◽  
Group Versus ◽  
Current Data ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Tnf Α ◽  
Low Activity

Background:: Ursolic Acid (UA) is a pentacyclic triterpenoid carboxylic acid which is extracted from plants. UA may enhance the effect of Resistance Training (RT) in human. Objective: Current research was designed to show the effect of High-Intensity Resistance Training (HIRT) in the presence or absence of UA on the serum levels of irisin, CRP, IL-6 and TNF-α in the low activity men. Method:: The study included twenty-two healthy male HIRT with placebo, supplementation, and HIRT in the presence of UA supplementation. The two groups received eight-week intervention including 2 sets of 8 exercises, with 8~10 repetitions at 70~75% of 1 repetition maximum and a 2 min rest interval between sets, performed 3 times/week. Placebo or UA orally was evaluated as 1 capsule 3 times/day during 8 weeks. The subsequent factors were measured post- and preintervention: C-Reactive Protein (CRP), Irisin, Tumor Necrotic Factor (TNF-α) and Interleukin-6 (IL-6). Results:: UA supplementation significantly increased the plasma levels of irisin in the HIRT+UA group versus the HIRT+P group (p<0.05). UA treatment also dramatically decreased the plasma levels of CRP, IL-6, and TNF-α in the HIRT+UA group versus the HIRT+P group (p<0.05). Conclusion:: The current data showed that UA-induced an increase in serum irisin and reduction of CRP, IL-6, and TNF-α may have beneficial effects as a chemical for increasing of the effects of HIRT in low activity men.

scholarly journals Salivary Biomarkers of Stress, Anxiety and Depression

2021 ◽  
Vol 10 (3) ◽  
pp. 517
Author(s):  
Sylwia Chojnowska ◽  
Iwona Ptaszyńska-Sarosiek ◽  
Alina Kępka ◽  
Małgorzata Knaś ◽  
Napoleon Waszkiewicz
Keyword(s):  
Salivary Cortisol ◽  
Depressive Disorders ◽  
Immunoglobulin A ◽  
Strong Relationship ◽  
Fibroblast Growth Factor 2 ◽  
Stress Axis ◽  
Anxiety And Depression ◽  
Salivary Biomarkers ◽  
Appropriate Treatment ◽  
As Stress

Stress, anxiety and depressive disorders are often characterized by the activation of the stress axis, which results in similar symptoms at some point in these disorders. These disorders are closely related to each other—they occur simultaneously or follow one another. The diagnosis of stress, anxiety and depression is not a perfect procedure currently—it is based on patient observation and an interview with the patient and their family. There are no laboratory tests that would dispel the doubts of the doctor making the diagnosis and allow the appropriate treatment to be implemented as soon as possible. Therefore, this study will review the components of saliva that could be helpful in the quick diagnosis of stress, anxiety and/or depression. Such potential salivary biomarkers could also be useful in monitoring the effectiveness of pharmacological treatment prescribed by a psychiatrist. The following are promising salivary biomarkers of stress, anxiety or depression: cortisol, immunoglobulin A (sIgA), lysozyme, melatonin, α-amylase (sAA), chromogranin A (CgA) and fibroblast growth factor 2 (FGF-2). To the best valuable potential salivary markers of stress, we can include cortisol, lysozyme, sAA and CgA. To differentiate depression from stress, salivary cortisol and melatonin can be helpful. Fluctuations in the concentrations of the above-mentioned substances in saliva indicate a particularly strong relationship with typical human psychological problems, such as stress, depression or anxiety.

scholarly journals The Efficacy of Convalescent Plasma Use in Critically Ill COVID-19 Patients

Medicina ◽  
2021 ◽  
Vol 57 (3) ◽  
pp. 257
Author(s):  
Livius Tirnea ◽  
Felix Bratosin ◽  
Iulia Vidican ◽  
Bianca Cerbu ◽  
Mirela Turaiche ◽  
...  
Keyword(s):  
Pilot Study ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Therapeutic Option ◽  
White Blood Cells ◽  
The United States ◽  
Plasma Therapy ◽  
Reactive Protein ◽  
United States Food ◽  
States Food

Background and Objectives: On 24 March 2020, the United States Food and Drug Administration (FDA) announced the approval of convalescent plasma therapy for critically ill patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as an emergency investigational new drug. This pilot study from Romania aimed to determine if convalescent plasma transfusion can be beneficial in the treatment of selected critically ill patients diagnosed with a SARS-CoV-2 infection. Materials and Methods: Donor and receiver eligibility for critically ill coronavirus disease 2019 (COVID-19) patients was based on Romanian guidelines issued at the time of the study. Here, we describe the evolution of a total of five eligible patients diagnosed with COVID-19 who received convalescent plasma (CP) in Romania. Results: In spite of our efforts and convalescent plasma administration, three of the five patients did not survive, while the other two recovered completely. Over the course of our five-day laboratory record, the surviving patients had significantly lower values for C-reactive protein, interleukin-6, and white blood cells. Conclusions: This pilot study provides insufficient evidence to determine the efficacy of convalescent plasma use as a therapeutic option for critically ill COVID-19 patients.

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