scholarly journals Anther Extrusion and Its Association with Fusarium Head Blight in CIMMYT Wheat Germplasm

Agronomy ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 47 ◽  
Author(s):  
Kaijie Xu ◽  
Xinyao He ◽  
Susanne Dreisigacker ◽  
Zhonghu He ◽  
Pawan K. Singh
Keyword(s):  
Fusarium Head Blight ◽  
Significant Negative Correlation ◽  
Wheat Breeding ◽  
Field Resistance ◽  
Head Blight ◽  
Morphological Marker ◽  
Breeding Lines ◽  
Anther Extrusion ◽  
Genetic Level ◽  
Fhb Resistance

Pronounced anther extrusion (AE) is associated with field resistance to Fusarium head blight (FHB), one of the most devastating diseases of wheat globally. In this study, two recombinant inbred line (RIL) populations were used to map quantitative trait loci (QTL) for AE and field FHB resistance and to investigate the association of both traits at the genetic level. Furthermore, two panels of International Maize and Wheat Improvement Center (CIMMYT) wheat breeding lines were evaluated to describe the phenotypic association between the two traits in detail. Highly significant negative correlation was identified between AE and FHB severity in the two populations and the two panels, with r-values ranging from 0.55 to 0.74. QTL analysis in the two RIL populations identified 12 QTL for AE and nine for FHB resistance, of which five QTL located on chromosomes 3BL, 4BS, 4DS, 5AL, and 5BL were associated with both AE and FHB, collectively explaining over 50% of phenotypic variation for FHB. The QTL on chromosomes 4BS, 4DS, 5AL, and 5BL were closely linked to Rht-B1, Rht-D1, Vrn-A1, and Vrn-B1 genes, respectively. In conclusion, AE is closely related to field FHB resistance and could be used as a morphological marker in wheat breeding for field FHB resistance.

scholarly journals Alien chromatin but not Fhb7 confers Fusarium head blight resistance in wheat breeding

2021 ◽  
Author(s):  
Xianrui Guo ◽  
Qinghua Shi ◽  
Jing Yuan ◽  
Mian Wang ◽  
Jing Wang ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Resistance Breeding ◽  
Wheat Breeding ◽  
Glutathione S Transferase ◽  
Head Blight ◽  
Fhb Resistance ◽  
Translocation Lines ◽  
High Level ◽  
Thinopyrum Elongatum

AbstractFusarium head blight (FHB), caused by Fusarium species, seriously threaten global wheat production. Three wheat-Th.elongatum FHB resistant translocation lines have been developed and used for breeding. Transcriptomic analysis identified a derivative glutathione S-transferase transcript T26102, which was homologous to Fhb7 and induced dramatically by Fusarium graminearum. Homologs of Fhb7 were detected in several genera in Triticeae, including Thinopyrum, Elymus, Leymus, Pseudoroegeria and Roegeria. Several wheat-Thinopyrum translocation lines carrying Fhb7 remain susceptible to FHB, and transgenic plants overexpressing the T26102 on different backgrounds did not improve the FHB resistance. Taken as a whole, we show the application of the chromatin derived from diploid Thinopyrum elongatum successfully conferring wheat with high level FHB resistance independent of the Fhb7.One Sentence SummaryThinopyrum elongatum chromatin from 7EL was successfully applied to wheat FHB resistance breeding, but the resistant gene other than the reported Fhb7 remained unknown.

scholarly journals Identification of a Novel Genomic Region Associated With Resistance to Fusarium Head Blight in Chinese Winter Wheat

2021 ◽  
Author(s):  
Yunzhe Zhao ◽  
Xinying Zhao ◽  
Mengqi Ji ◽  
Wenqi Fang ◽  
Hong Guo ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Fusarium Head Blight ◽  
Genome Wide Association Study ◽  
Wheat Breeding ◽  
Scab Resistance ◽  
Yield Reduction ◽  
Transferase Activity ◽  
Head Blight ◽  
A Genome ◽  
Fhb Resistance

Abstract Background: Fusarium head blight (FHB) is a disease affecting wheat spikes caused by Fusarium species, which leads to cases of severe yield reduction and seed contamination. Therefore, identifying resistance genes from various sources is always of importance to wheat breeders. In this study, a genome-wide association study (GWAS) focusing on FHB using a high-density genetic map constructed with 90K single nucleotide polymorphism (SNP) arrays in a panel of 205 elite winter wheat accessions, was conducted in 3 environments. Results: Sixty-six significant marker–trait associations (MTAs) were identified (P<0.001) on fifteen chromosomes explaining 5.4–11.2% of the phenotypic variation therein. Some important new genomic regions involving FHB resistance were found on chromosomes 2A, 3B, 5B, 6A, and 7B. On chromosome 7B, 6 MTAs at 92 genetic positions were found in 2 environments. Moreover, there were 11 MTAs consistently associated with diseased spikelet rate and diseased rachis rate as pleiotropic effect loci. Eight new candidate genes of FHB resistance were predicated in wheat. Of which, three genes: TraesCS5D01G006700, TraesCS6A02G013600, and TraesCS7B02G370700 on chromosome 5DS, 6AS, and 7BL, respectively, were important in defending against FHB by regulating chitinase activity, calcium ion binding, intramolecular transferase activity, and UDP-glycosyltransferase activity in wheat. In addition, a total of six excellent alleles associated with wheat scab resistance were discovered. Conclusion: These results provide important genes/loci for enhancing FHB resistance in wheat breeding populations by marker-assisted selection.

scholarly journals A Quantitative Proteomics View on the Function of Qfhb1, a Major QTL for Fusarium Head Blight Resistance in Wheat

Pathogens ◽  
2018 ◽  
Vol 7 (3) ◽  
pp. 58 ◽  
Author(s):  
Moustafa Eldakak ◽  
Aayudh Das ◽  
Yongbin Zhuang ◽  
Jai Rohila ◽  
Karl Glover ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Starch Synthesis ◽  
Resistance Mechanism ◽  
Fusarium Head Blight Resistance ◽  
Defense Responses ◽  
Wheat Breeding ◽  
Host Cells ◽  
Head Blight ◽  
Fhb Resistance ◽  
Trait Locus

Fusarium head blight (FHB) is a highly detrimental disease of wheat. A quantitative trait locus for FHB resistance, Qfhb1, is the most utilized source of resistance in wheat-breeding programs, but very little is known about its resistance mechanism. In this study, we elucidated a prospective FHB resistance mechanism by investigating the proteomic signatures of Qfhb1 in a pair of contrasting wheat near-isogenic lines (NIL) after 24 h of inoculation of wheat florets by Fusarium graminearum. Statistical comparisons of the abundances of protein spots on the 2D-DIGE gels of contrasting NILs (fhb1+ NIL = Qfhb1 present; fhb1- NIL = Qfhb1 absent) enabled us to select 80 high-ranking differentially accumulated protein (DAP) spots. An additional evaluation confirmed that the DAP spots were specific to the spikelet from fhb1- NIL (50 spots), and fhb1+ NIL (seven spots). The proteomic data also suggest that the absence of Qfhb1 makes the fhb1- NIL vulnerable to Fusarium attack by constitutively impairing several mechanisms including sucrose homeostasis by enhancing starch synthesis from sucrose. In the absence of Qfhb1, Fusarium inoculations severely damaged photosynthetic machinery; altered the metabolism of carbohydrates, nitrogen and phenylpropanoids; disrupted the balance of proton gradients across relevant membranes; disturbed the homeostasis of many important signaling molecules induced the mobility of cellular repair; and reduced translational activities. These changes in the fhb1- NIL led to strong defense responses centered on the hypersensitive response (HSR), resulting in infected cells suicide and the consequent initiation of FHB development. Therefore, the results of this study suggest that Qfhb1 largely functions to either alleviate HSR or to manipulate the host cells to not respond to Fusarium infection.

scholarly journals Epigenetic regulation of gene expression improves Fusarium head blight resistance in durum wheat

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jitendra Kumar ◽  
Krishan M. Rai ◽  
Seyedmostafa Pirseyedi ◽  
Elias M. Elias ◽  
Steven Xu ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Regulation Of Gene Expression ◽  
Fusarium Head Blight Resistance ◽  
Mapk Signaling ◽  
Head Blight ◽  
Breeding Lines ◽  
Significant Difference ◽  
Plant Pathogen Interaction ◽  
Fhb Resistance ◽  
Parental Lines

Abstract Eight advanced durum-breeding lines were treated with 5-methyl-azacytidine to test the feasibility of generating sources of Fusarium head blight (FHB) resistance. Of the 800 treated seeds, 415 germinated and were advanced up to four (M4) generations by selfing. Thirty-two of the resulting 415 M4 lines were selected following preliminary screening and were further tested for FHB resistance for three years at two field locations, and in the greenhouse. Five of the 32 M4 lines showed less than 30% disease severity, as compared to the parental lines and susceptible checks. Fusarium-damaged kernels and deoxynivalenol analyses supported the findings of the field and greenhouse disease assessments. Two of the most resistant M4 lines were crossed to a susceptible parent, advanced to third generation (BC1:F3) and were tested for stability and inheritance of the resistance. About, one third of the BC1:F3 lines showed FHB resistance similar to their M4 parents. The overall methylation levels (%) were compared using FASTmC method, which did not show a significant difference between M4 and parental lines. However, transcriptome analysis of one M4 line revealed significant number of differentially expressed genes related to biosynthesis of secondary metabolites, MAPK signaling, photosynthesis, starch and sucrose metabolism, plant hormone signal transduction and plant-pathogen interaction pathways, which may have helped in improved FHB resistance.

scholarly journals Evaluation of Canadian barley breeding lines for Fusarium head blight resistance

2015 ◽  
Vol 95 (5) ◽  
pp. 923-929 ◽  
Author(s):  
Xinyao He ◽  
Mohamed Osman ◽  
James Helm ◽  
Flavio Capettini ◽  
Pawan K. Singh
Keyword(s):  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Correlation Coefficients ◽  
Blight Resistance ◽  
Head Blight ◽  
Breeding Programs ◽  
Breeding Lines ◽  
Barley Breeding ◽  
Days To Heading ◽  
Fhb Resistance

He, X., Osman, M., Helm, J., Capettini, F. and Singh, P. K. 2015. Evaluation of Canadian barley breeding lines for Fusarium head blight resistance. Can. J. Plant Sci. 95: 923–929. Fusarium head blight (FHB) is a major challenge to the successful production of barley in Canada, as well as for end-users such as the malting and brewing industries. Due to the quantitative inheritance of FHB resistance, continuous effort is required to identify breeding lines with improved FHB resistance and incorporate them into crossing schemes to enhance FHB resistance. In the present study, 402 advanced breeding lines from Alberta, Canada, were evaluated in the FHB screening nursery at CIMMYT, Mexico. In 2011 and 2012, FHB incidence was measured on a scale of 1 to 4 to eliminate the most susceptible lines. In 2013 and 2014, 181 lines with the lowest disease scores in the previous 2 yr were tested in replicated experiments for field FHB index, Fusarium-damaged kernels, and deoxynivalenol content. Agronomic and morphological traits, specifically days to heading, plant height, and row and hull types were also evaluated in relations to FHB parameters. Correlation coefficients among the three FHB parameters in both 2013 and 2014 were all significant at P<0.0001, ranging from 0.36 to 0.63. Additional correlation analysis showed that late-maturing, tall, and two-row lines tended to have lower disease, whereas hull type did not show a significant correlation with FHB. Several lines with high and stable FHB resistance similar to that of the resistant checks were identified. These could be used in breeding programs as resistance sources or be registered as new cultivars if their overall attributes meet commercial standards.

scholarly journals Molecular Cytogenetic Characterization and Fusarium Head Blight of Five Wheat Thinopyrum Intermedium Partial Amphiploids

2020 ◽  
Author(s):  
Hui Wang ◽  
Shuwei Cheng ◽  
Yue Shi ◽  
Shuxin Zhang ◽  
Wei Yan ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Wheat Breeding ◽  
Thinopyrum Intermedium ◽  
Head Blight ◽  
Multicolor Fish ◽  
Wheat Diseases ◽  
Cytogenetic Characterization ◽  
Fhb Resistance ◽  
Partial Amphiploids ◽  
Best Disease

Abstract Background: Partial amphiploids created by crossing octoploid tritelytrigia and Thinopyrum intermedium are important intermediates in wheat breeding because of their resistance to major wheat diseases. We examined the chromosome compositions of five wheat Th. intermedium partial amphiploids using GISH and multicolor FISH. Results: The result revealed that five lines had 10 14 J genome chromosomes from Th. intermedium and 42 common wheat chromosomes, using the J genomic DNA from Th. bessarabicum and the oligo probes pAs1 1 , pAs1 3 , AFA 4 , GAA ) 10, and pSc119.2 1 . Five lines resembled the parent Ganmai 8 but had better protein contents. P rotein contents of t wo lines HS2 2 and HS2 5 were up to more than 20%. Evaluation of Fusarium head blight (FHB) resistance revealed that the percent of symptomatic spikelets (PSS) of these lines were below 30%. Lines HS2 2, HS2 4, HS2 5, and HS2 16 were less than 20%. Line HS2 5 with 14 J genome chromosomes from Th. intermedium showed the best disease resistance, with PSS values of 10.8% and 16.6% in 2016 and 2017, respectively. Conclusions: New wheat Th. intermedium amphiploids with the J genome chromosomes were identified and can be considered as a valuable source of FHB resistance in wheat breeding.

Genetic variation for resistance to Fusarium head blight in winter durum material

2014 ◽  
Vol 65 (1) ◽  
pp. 46 ◽  
Author(s):  
Thomas Miedaner ◽  
Carl Friedrich Horst Longin
Keyword(s):  
Genetic Variation ◽  
Fusarium Head Blight ◽  
Plant Height ◽  
Fusarium Culmorum ◽  
Heading Time ◽  
Head Blight ◽  
Breeding Lines ◽  
Breeding Material ◽  
Quantitative Genetic ◽  
Fhb Resistance

Durum is one of the most susceptible cereals to infection with Fusarium head blight (FHB) due to the lack of resistance sources. Data on the genetic variation and heritability of FHB in elite durum, and especially the new evolving winter durum, are lacking. Thus, we compared 105 elite, winter durum breeding lines with an international collection of 62 old and new winter durum cultivars. We evaluated the development of FHB after inoculation by Fusarium culmorum, heading time, and plant height at three field environments in Germany. Significant genetic variation for FHB was identified in the elite breeding material as well as in the collection. Mean FHB rating was normally distributed with a heritability of >0.7 for both sets, indicating the quantitative genetic nature of the trait. Taking heading time, plant height, and FHB resistance into account, the most interesting genotypes were identified in the elite breeding material. Consequences for the ongoing global efforts for improvement of FHB resistance of durum are discussed.

Genetic trends in Fusarium head blight resistance due to 20 years of winter wheat breeding and cooperative testing in the Northern US.

Plant Disease ◽  
2021 ◽  
Author(s):  
Rupesh Gaire ◽  
Clay Sneller ◽  
Gina Brown-Guedira ◽  
David A. Van Sanford ◽  
Mohsen Mohammadi ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Fusarium Head Blight ◽  
Mixed Model ◽  
Fusarium Head Blight Resistance ◽  
Wheat Breeding ◽  
Vital Role ◽  
Head Blight ◽  
Genetic Progress ◽  
Fhb Resistance ◽  
Genetic Trends

Fusarium head blight (FHB) is a devastating disease of wheat and barley. In the US, a significant long-term investment in breeding FHB resistant cultivars began after the 1990s. However, to this date, no study has been performed to understand and monitor the rate of genetic progress in FHB resistance as a result of this investment. Using 20 years of data (1998 to 2018) from the Northern Uniform (NU) and Preliminarily Northern Uniform (PNU) winter wheat scab nurseries which consisted of 1068 genotypes originating from 9 different institutions, we studied the genetic trends in FHB resistance within the northern soft red winter wheat growing region using mixed model analyses. For the FHB resistance traits incidence, severity, Fusarium damaged kernels (FDK), and deoxynivalenol content, the rate of genetic gain in disease resistance was estimated to be 0.30 ± 0.1, 0.60 ± 0.09, 0.37 ± 0.11 points per year, and 0.11 ± 0.05 ppm per year, respectively. Among the five FHB resistance QTL assayed for test entries from 2012 to 2018, the frequencies of favorable alleles from Fhb 2DL Wuhan1 W14, Fhb Ernie 3Bc, and Fhb 5A Ning7840 was close to zero across the years. The frequency of the favorable at Fhb1 and Fhb 5A Ernie ranged from 0.08 to 0.33 and 0.06 to 0.20 respectively across years, and there was no trend in changes in allele frequencies over years. Overall, this study showed that substantial genetic progress has been made towards improving resistance to FHB. It is apparent that the current investment in public wheat breeding for FHB resistance is achieving results and will continue to play a vital role in reducing FHB levels in growers’ fields.

Multiplexed PCR to screen for a major QTL carrying fusarium head blight resistance in Sumai-3 wheat

2006 ◽  
Vol 86 (3) ◽  
pp. 711-716 ◽  
Author(s):  
M. A. Matus-Cádiz ◽  
C. J. Pozniak ◽  
G. R. Hughes ◽  
P. Hucl
Keyword(s):  
Fusarium Head Blight ◽  
Fusarium Head Blight Resistance ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Head Blight ◽  
Breeding Lines ◽  
Multiplexed Pcr ◽  
Trait Locus ◽  
Advanced Generation ◽  
Sumai 3

After the initial identification of microsatellites linked to economically important traits of interest, the additional investment to simplify the screening procedure for more routine use is of interest to plant breeders. The objective was to simplify an acrylamide gel based marker-assisted selection (MAS) method to facilitate high-through put screening for Qfhs.ndsu-3BS, a major quantitative trait locus carrying fusarium head blight (FHB) resistance in Sumai-3 wheat (Triticum aestivum L.). Method simplifications included incorporating the use of (1) 96-well DNA extractions, (2) multiplexed PCR reactions using microsatellite primers gwm493 and gwm533, and (3) agarose gels. Our modified FHB-MAS method was verified by screening six resistant (Sumai-3, ND2710, McVey, BacUp, HY644, and Alsen) and 52 susceptible parents and subsequently used to screen 5567 common wheat breeding lines developed from Sumai-3 derivatives. This simple and rapid method allows for the screening of 1000 lines per week, which can be used to skew segregating populations towards more resistant types and thereby advance primarily early and advanced generation lines that carry at least the major FHB QTL. Key words: PCR, Fusarium, microsatellites, gwm493, gwm533, Triticum

scholarly journals Mapping of Major Fusarium Head Blight Resistance from Canadian Wheat cv. AAC Tenacious

2020 ◽  
Vol 21 (12) ◽  
pp. 4497 ◽  
Author(s):  
Raman Dhariwal ◽  
Maria A. Henriquez ◽  
Colin Hiebert ◽  
Curt A. McCartney ◽  
Harpinder S. Randhawa
Keyword(s):  
Fusarium Head Blight ◽  
Agronomic Traits ◽  
Fusarium Head Blight Resistance ◽  
Wheat Breeding ◽  
Head Blight ◽  
Visual Rating ◽  
Wheat Population ◽  
Major Bottleneck ◽  
Yield Quality ◽  
Fhb Resistance

Fusarium head blight (FHB) is one of the most devastating wheat disease due to its direct detrimental effects on grain-yield, quality and marketability. Resistant cultivars offer the most effective approach to manage FHB; however, the lack of different resistance resources is still a major bottleneck for wheat breeding programs. To identify and dissect FHB resistance, a doubled haploid wheat population produced from the Canadian spring wheat cvs AAC Innova and AAC Tenacious was phenotyped for FHB response variables incidence and severity, visual rating index (VRI), deoxynivalenol (DON) content, and agronomic traits days to anthesis (DTA) and plant height (PHT), followed by single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) marker genotyping. A high-density map was constructed consisting of 10,328 markers, mapped on all 21 chromosomes with a map density of 0.35 cM/marker. Together, two major quantitative trait loci for FHB resistance were identified on chromosome 2D from AAC Tenacious; one of these loci on 2DS also colocated with loci for DTA and PHT. Another major locus for PHT, which cosegregates with locus for low DON, was also identified along with many minor and epistatic loci. QTL identified from AAC Tenacious may be useful to pyramid FHB resistance.

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