scholarly journals Herbicidal Effects of Ethyl Acetate Extracts of Billygoat Weed (Ageratum conyzoides L.) on Spiny Amaranth (Amaranthus spinosus L.) Growth

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1991
Author(s):  
Gina Erida ◽  
Nurdin Saidi ◽  
Hasanuddin Hasanuddin ◽  
Syafruddin Syafruddin
Keyword(s):  
Ethyl Acetate ◽  
Acid Ethyl Ester ◽  
Dry Weight ◽  
Positive Control ◽  
Control Agent ◽  
Negative Control ◽  
Herbicidal Activity ◽  
Ageratum Conyzoides ◽  
Tetradecanoic Acid ◽  
Amaranthus Spinosus

This study aimed to evaluate the herbicidal activity of ethyl acetate leaf extract of Ageratum conyzoides L. at different subfractions on Amaranthus spinosus L. The leaves of A. conyzoides were sequentially extracted with n-hexane and ethyl acetate respectively and fractionated by chromatography column. The extracts were applied to A. spinosus in pot assays at a concentration of 5%, 10% and 15%. We applied A synthetic herbicide (2,4-D at 0.686 kg a.i. ha−1) for positive control and distilled water for negative control. The A. conyzoides extracts strongly differed in their effect on weed control, shoot and root dry weight and root length of A. spinosus. The most inhibition on A. spinosus growth caused by application of ethyl acetate of A. conyzoides extracts subfraction A by 10% concentration can cause 100% destruction and subfraction B were 95% which both of them cause strongest death on A. spinosus compared with synthetic herbicide (2, 4-D) (23.33%) at 1 Day After Application, while subfraction C and D were not effective. Main constituents identified by GC-MS in subfraction A extract were tetradecanoic acid, ethyl ester (10.26%), precocene II (9.39%), octadecanal (8.23%), 9,12,15-octatadecatrienoic, methyl ester (7.32%), 10-heneicosene (c,t) (5.19%) and neophytadiene (5.09%); in subfraction B were 1-octadecyne (38.57%), phytol (11.24%), di-tert-utylphosphine-d (5.17%) and 1-hexadecine (4.08%); in subfraction C were allobarbital (8.53%), octadecanal (12.69%), and bannamurpanin (26.01%) and octadecanal (30.52%), bannamurpanin (24.06%), 1,8-cineole (15.75%), trans-dodec-5enal (12.28%) and phytol (8.26%) in subfraction D. The ethyl acetate extract subfraction A and B concentration 10% proved the promising control agent against A. spinosus.

Effect of Chayote (Sechium Edule Jacq. Swartz) Extract on Level of Interleukin-8 in Wistar Rats with Aspirin-Induced Gastritis

2020 ◽  
Author(s):  
Hendrika Andriana Silitonga ◽  
◽  
Gontar Alamsyah Siregar ◽  
Rosita Juwita Sembiring ◽  
Marline Nainggolan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Interleukin 8 ◽  
Control Group ◽  
Sechium Edule

ABSTRACT Background: Recent studies showed that Interleukin-8 (IL-8), activated cytokine immune response which plays an important role in the development of acute and chronic gastritis. Harmless anti-inflammatory therapeutic alternatives have been proposed, for example, the consumption of Sechium Edule Jacq. Swartz (chayote). Antioxidant (flavonoid) and cell regeneration (alkaloid) agents were found in chayote. This study aimed to determine the effect of chayote Sechium Edule Jacq. Swartz extracts on the level of IL-8 in Wistar rats with aspirin- induced gastritis. Subjects and Method: This was a randomized controlled trial (RCT) conducted at the laboratory of Mathematics and Natural Science, Universitas Sumatra Utara from January to February 2020. A total of 35 male Wistar rats was selected for this study and randomly allocated into 7 groups: (1) Negative control; (2) Positive control; (3) 100 mg/ kg BW chayote ethanol extract ; (4) 200 mg/kg BW chayote ethanol extract; (5) 100 mg/ kg BW chayote ethyl acetate fraction; (6) 200 mg/kg BW chayote ethyl acetate fraction; and (7) 20 mg omeprazole. The rats in positive control and treatment groups were induced with aspirin (200mg/ kg BW). The negative control group received no intervention. The dependent variable was level of IL-8 measured by ELISA. The independent variables were treatment status. The data were analyzed by One Way Anova and post hoc test. Results: The mean differences of IL-8 level were not statistically significant between study groups (p= 0.327). Mean of IL-8 level was higher in positive control group (Mean= 160.80; SD= 6.90) than in negative control group (Mean= 141.20; SD= 10.98). The lowest IL-8 level was in 100mg/ kg BW chayote ethanol extract group (Mean= 149.94; SD= 40.4), followed by 200mg/ kg BW (Mean= 152.4; SD= 30.73) and 100mg/ kg BW (Mean= 164.60; SD= 25.04) chayote ethyl acetate fraction groups, 20 mg omeprazole group (Mean= 170.60; SD= 21.58), and 200 mg/ kg BW chayote ethanol extract group (Mean= 176.80; SD= 10.98). Conclusion: The low dose (100mg/ kg BW) chayote ethanol extract has the most potential antiinflammation effect on in vitro gastritis with the lowest IL-8 level of all doses of chayote ethanol extract, chayote ethyl acetate fraction, and omeprazole. Keywords: antiinflammation, IL-8, chayote ethanol extract, ethyl acetate fraction, omeprazole, aspirin induced gastritis Correspondence: Hendrika Andriana Silitonga. Department of Histology, Faculty of Medicine, Universitas Methodist Indonesia. Email: [email protected]. Mobile: +6281361430688. DOI: https://doi.org/10.26911/the7thicph.05.35

scholarly journals UJI AKTIVITAS ANTIBAKTERI ISOLAT SENYAWA GOLONGAN TERPENOID DARI BIJI PEPAYA (Carica papaya L.) TERHADAP Staphylococcus aureus SECARA IN VIVO PADA KELINCI JANTAN

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Keyword(s):  
Staphylococcus Aureus ◽  
Ethyl Acetate ◽  
Carica Papaya ◽  
Test Group ◽  
Positive Control ◽  
Negative Control ◽  
Test Substance ◽  
Color Test ◽  
Group 15

research on the isolation of terpenoid class of compounds from the seeds of papaya (Carica papaya L.) and test its activity against Staphylococcus aureus in vivo in male rabbits. This research aims to prove that the terpenoid compounds isolated from the seeds of papaya (Carica papaya L.) can inhibit the growth of Staphylococcus aureus in vivo. Separation of terpenoid compounds by column chromatography ((eluent n-hexane: ethyl acetate: ethanol)) resulted in 25 eluates, and then merged based on the results of identification by TLC ((nhexane: ethyl acetate (8: 2)) and the color test reagent Lieburmann -Burchard produce 5 fraction groups. fraction D showed positive terpenoids with Rf 0.75 and the color purple with Lieburmann-Burchard reagent. study using 15 rabbits were divided into 5 groups: P1 (negative control), P2 (positive control) , P3 (5% of the test group), P4 (10% of the test group), P5 (test group 15%). each group was given the intracutaneous Staphylococcus aureus as 0,2ml on the backs of rabbits. Having symptoms of infection each group was given the test substance 3 times a day topically, the observed parameter is the diameter of the wound, and histopathological observations performed on days 3,6 and 9 Analysis of the results of research conducted using ANSIRA showed highly significant differences between groups (p <0.05). Then proceed with the analysis of the results of the analysis HSD test showed highly significant differences in the test group 5% to 10% of the test group and the test group 15%. Isolates terpenoid class of compounds from the seeds of papaya (Carica Papaya L.) with a concentration of 10% and 15% can inhibit the growth of Staphylococcus aureus. Keywords: Antibacterial, Staphylococcus aureus, male rabbits

scholarly journals Uji Efektivitas Bioherbisida Ekstrak Etil Asetat Babadotan (Ageratum conyzoides L.) Dari Berbagai Ketinggian Tempat Dan Konsentrasi Terhadap Pertumbuhan Gulma Bayam Duri (Amaranthus spinosus L.)

2020 ◽  
Vol 3 (4) ◽  
pp. 34-42
Author(s):  
Zainuddin Zainuddin ◽  
Siti Hafsah ◽  
Gina Erida
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Ethyl Acetate Fraction ◽  
High Rate ◽  
Ageratum Conyzoides ◽  
Amaranthus Spinosus ◽  
Number Of Leaves ◽  
Materials Used ◽  
Banda Aceh ◽  
Ethyl Acetate Extracts

Abstrak. Penelitian ini bertujuan untuk menguji aktivitas ekstrak etil asetat babadotan dari berbagai ketinggian tempat dan konsentrasi terhadap pertumbuhan gulma bayam duri. Penelitian ini dilaksanakan di Laboratorium Ilmu Gulma Program Studi Agroteknologi dan Kebun Percobaan Fakultas Pertanian Universitas Syiah Kuala Banda Aceh, serta Laboratorium Analisis Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA), sejak September 2017– Januari 2018. Bahan yang digunakan adalah benih bayam duri, yang diperoleh dari Kecamatan Delima Kabupaten Pidie dan daun babadotan yang diperoleh dari 3 daerah dengan ketinggian yang berbeda yaitu Kecamatan Indrapuri Kabupaten Aceh Besar (19 mdpl), Kecamatan Lembah Seulawah Saree Kabupaten Aceh Besar (261 mdpl) dan Kecamatan Takengon Kabupaten Aceh Tengah (1259 mdpl) dengan konsentrasi 5, 10 dan 15% sebagai bioherbisida. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) pola faktorial 3  3 dengan 3 kali ulangan. Faktor pertama yaitu ketinggian tempat dengan 3 taraf dan faktor kedua konsentrasi dengan 3 taraf. Ekstrak yang dihasilkan pada ketinggian tempat yang berbeda dan konsentrasi ekstrak etil asetat berpengaruh nyata terhadap laju tinggi, jumlah daun, diameter batang, persentase pengendalian dan panjang akar. Terdapat interaksi antara ketinggian tempat dan konsentrasi terhadap persentase pengendalian pada 7 dan 14 HSA. Ekstrak yang dihasilkan pada ketinggian  9 mdpl dengan konsentrasi 10% dapat mengendalikan gulma bayam duri hingga 100% pada 7 HSA.The Test of Effectiveness Whiteweed Ethyl Acetate Extract (Ageratum conyzoides L.) from Various Altitudes And Concentration Against the Growth of Spiny Amaranth Weed (Amaranthus spinosus L.)Abstract. This study aims to examine the activity of ethyl acetate fraction of whiteweed extract from various altitudes and concentration againts the growth of spiny amaranth. This  research was conducted at Laboratory of Weed Science, Agrotechnology Department, and Experimental Garden, Agriculture Faculty, Syiah Kuala University Banda Aceh, and Laboratory of Chemical Analysis Mathematics Faculty and Natural Science, from September 2017 to January 2018. The materials used is spiny amaranth seed that obtained from Subdistrict of Delima, District of Pidie and babadotan leaves obtained from three regions with different altitude that is Subdistrict of Indrapuri, District of Aceh Besar (19 masl), Subdistrict of Subdistrict of, District of Aceh Besar (261 masl) and Subdistrict of Takengon District of Aceh Tengah (1259 masl) with each concentration 5, 10 and 15% as bioherbicide. This research using Randomized Completely Design with Factorial pattern which has 3 × 3 treatments and 3 replication. The first faktor is altitude and second is concentration of ethyl acetate extract. Ethyl acetate extracts that produced in different altitudes and concentrations were significantly influence the high rate of spiny amaranth, number of leaves, stem diameter, control percentage and root length. There is an interaction between altitude and concentration on the percentage of control at 7 and 14 HSA. Extracts produced at an altitude of 9 masl with a concentration of 10% can control spinach weeds up to 100% at 7 HSA.

scholarly journals Pengaruh Induksi Resistensi oleh Khamir Candida tropicalis terhadap Pertumbuhan Tanaman Cabai Terinfeksi Colletotrichum acutatum

Agrikultura ◽  
2019 ◽  
Vol 30 (1) ◽  
pp. 17
Author(s):  
Sri Hartati ◽  
Linda Tarina ◽  
Endah Yulia ◽  
Luciana Djaya
Keyword(s):  
Plant Height ◽  
Induced Resistance ◽  
Candida Tropicalis ◽  
Yield Loss ◽  
Biocontrol Agent ◽  
Dry Weight ◽  
Positive Control ◽  
Negative Control ◽  
Leaf Number ◽  
Colletotrichum Acutatum

ABSTRACTEffect of induced resistance by Candida tropicalis on the growth of chili plants infected by Colletotrichum acutatumColletotrichum acutatum is a major pathogen of chili that causes a big yield loss. Candida tropicalis has been reported as a biocontrol agent antagonistic to C. acutatum. The research was objected tofind out the effect of induced resistance by C. tropicalis on the growth of chili plants infected by C. acutatum. The application of the yeast was carried out by seed dipping and seedling spraying methods. As treatments, C. acutatum, the pathogen was inoculated at different time, i.e. A = inoculation at 3 days after the induction (dai) by C. tropicalis, B = inoculation at 7 dai, C = inoculation at 10 dai, D = positive control on inoculation at 3 dai, E = positive control on inoculation at 7 dai, F = positive control on inoculation at 10 dai, G = negative control with no induction nor inoculation. The results showed that the induction by C. tropicalis was able to increase the plant height, leaf number, and dry weight. The highest increase of plant height of 30.72% was caused by induction of C. tropicalis inoculated at 7 dai. Induction of C. tropicalis inoculated at 3 dai increased the leaf number by 49.47%, and the plant dry weight by 50%. C. tropicalis has a potency to be developed as plant growth inducer.Keywords: Antagonist, Inoculation, Fungi, YeastABSTRAKJamur Colletotrichum acutatum merupakan patogen penyebab kehilangan hasil panen yang cukup besar pada tanaman cabai. Salah satu mikrob antagonis jamur ini yang telah dilaporkan adalah khamir Candida tropicalis. Penelitian ini bertujuan untuk mengetahui pengaruh induksi resistensioleh C. tropicalis terhadap pertumbuhan tanaman cabai terinfeksi C. acutatum. Penelitian dilaksanakan dengan menggunakan teknik perendaman benih dan penyiraman bibit tanaman cabaimenggunakan suspensi C. tropicalis dengan waktu inokulasi patogen C. acutatum yang berbeda. Perlakuan tersebut adalah A = Inokulasi C. acutatum 3 hari setelah perlakuan induksi C. tropicalis (hsp), B = Inokulasi C. acutatum 7 hsp, C = Inokulasi C. acutatum 10 hsp, D = Kontrol (+) dengan inokulasi C. acutatum 3 hsp, E = Kontrol (+) dengan inokulasi C. acutatum 7 hsp, F = Kontrol (+) dengan inokulasi C. acutatum 10 hsp, G = Kontrol (-) tanpa induksi dan inokulasi. Hasil penelitianmenunjukkan bahwa perlakuan induksi resistensi oleh khamir C. tropicalis mampu meningkatkan tinggi, jumlah daun, dan bobot kering tanaman cabai. Kemampuan meningkatkan tinggi tanamancabai tertinggi terjadi pada perlakuan C. tropicalis dengan waktu inokulasi 7 hsp yaitu sebesar 30,72%. Perlakuan khamir C. tropicalis dengan inokulasi patogen 3 hsp mampu meningkatkan jumlah daun tanaman cabai sebesar 49,47%. Perlakuan C. tropicalis dengan waktu inokulasi 3 hsp mampu meningkatkan bobot kering tanaman sebesar 50%. Khamir C. tropicalis berpotensi untuk dikembangkan sebagai agens pemacu pertumbuhan tanaman.Kata Kunci: Antagonis, Inokulasi, Jamur, Patogen

scholarly journals Effectivity of Trichoderma harzianum as Bio-fungicide Against Moler Disease and Bio-stimulator of Shallot Growth

2020 ◽  
Vol 1 (1) ◽  
pp. 12
Author(s):  
Icha Rusita ◽  
Hadi Sasongko
Keyword(s):  
Trichoderma Harzianum ◽  
Dry Weight ◽  
Positive Control ◽  
Negative Control ◽  
Correlation Pattern ◽  
Randomized Design ◽  
Significant Difference ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Completely Randomized Design

Moler disease is the primary disease that disturbs the shallot’ cultivation. Hence, it is beneficial to have preventive measures to mitigate the risk and to improve growth. The research objective is to know the effectivity of Trichoderma harzianum suspension as a bio-fungicide and bio-stimulator. The study used seven treatments, i.e., immersion of shallot seeds on negative control (distilled water), positive control (synthetic fungicide+ZPT 1g/liter), T.harzianum suspension with different concentrations of 2 ppm, 4 ppm, 6 ppm, 8 ppm, and 10 ppm, assigned in a Completely Randomized Design (CRD). Treatment that results in a significant level will be continued to the Least Significance Different (LSD) 5% test. The observed variables are the intensity of moler disease and the growth of shallot seeds. Experiment results show the significant difference between treatments. The optimal treatment to reduce the percentage of moler disease intensity is the immersion of shallot seeds in 10 ppm-concentrated T.harzianum suspension, while to improve the shallot growth is in 8 ppm-concentrated T.harzianum suspension. The intensity of moler disease has a negative correlation to the plant’s height and leaf’s diameter but shows no correlation pattern to the number of leaves, wet weight, dry weight, and length of roots.

scholarly journals Bioprospecting Cultivated Tropical Green Algae, Caulerpa racemosa (Forsskal) J. Agardh: A Perspective on Nutritional Properties, Antioxidative Capacity and Anti-Diabetic Potential

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1313
Author(s):  
Abdul Qudus B Aroyehun ◽  
Shariza Abdul Razak ◽  
Kishneth Palaniveloo ◽  
Thilahgavani Nagappan ◽  
Nur Suraiza Nabila Rahmah ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Protein Quality ◽  
Ethyl Acetate Extract ◽  
Folk Medicine ◽  
Dry Weight ◽  
Positive Control ◽  
Total Phenolic ◽  
Caulerpa Racemosa ◽  
Glucose Levels ◽  
Green Seaweed

Caulerpa racemosa (Forsskal) J. Agardh is a green seaweed used as food and folk medicine since ancient times in the Indo-Pacific region, particularly in southeast Asia. In this study, the proximate nutrient composition, phytochemical, anti-oxidant and anti-diabetic properties of sea grape C. racemosa collected from culture fishponds in Johor, Malaysia were analysed. The contents (dry weight basis) of carbohydrate, crude protein, crude lipids, ash and caloric value obtained were 33.42 ± 1.34%, 20.27 ± 0.14%, 4.20 ± 0.32%, 28.25 ± 0.27% and 2544.67 ± 7.04 cal g−1, respectively. The amino acid score (AAs) and biological protein value (213.43 mg g−1) indicated that C. racemosa presented a better protein quality. The most abundant fatty acids were C16:0 (palmitic acid: 63.27%), followed by C18:1 (oleic acid: 5.80%), and C18:2 ῳ6 (linoleic acid: 5.33%). The analysis of the ash content indicated that essential minerals and trace elements, such as Ca, Fe, and Mn, were present in the seaweed. The total phenolic content (TPC) and total flavonoid content (TFC) observed in the ethyl acetate extract were 17.88 ± 0.78 mg GAE g−1 and 59.43 ± 2.45 mg QE g−1, respectively. The ethyl acetate extract of C. racemosa demonstrated notable anti-diabetic activity in diabetic induced rats. The low (100 mg kg−1) and high (200 mg kg−1) doses of cultivated C. racemosa extract exhibited a significant decrease (p < 0.05) in blood glucose levels while preventing weight loss, reducing plasma AST, ALT levels as a sign of hepatoprotective effect and recording albumin levels similar to positive control in diabetic induced rats. The results support the usefulness of cultivated C. racemosa as a potential functional food.

scholarly journals THE BENEFITS OF ACTIVE COMPOUNDS IN KALANCHOE PINNATA (LMK) PERS ETHYL ACETATE FRACTION ON LUPUS ARTHRITIS MICE

2017 ◽  
Vol 10 (11) ◽  
pp. 199 ◽  
Author(s):  
Niken Indriyanti ◽  
Joewono Soeroso ◽  
Junaidi Khotib
Keyword(s):  
Flow Cytometry ◽  
Dendritic Cells ◽  
Ethyl Acetate ◽  
Joint Damage ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Negative Control ◽  
Tissue Structure ◽  
Active Compounds ◽  
Kalanchoe Pinnata

Ethyl acetate fraction of Kalanchoe pinnata (Lmk) Pers leaves (EF-KP) is a potential material to be developed for lupus treatment. The aim of this research was to measure the effects of the EF-KP on lupus arthritis mice.  The research was performed by testing of CD123+IFN-α+ dendritic cells and CD68+IL-6+ macrophages as the main biomarkers by using flow cytometry methods, and then the outcomes were directly observed in the joint’s tissue structure. The results of the research were analyzed by using statistics. The EF-KP reduces the relative percentages of CD123+IFN-α+ dendritic cells significantly (p<0.05) with the percentage of 32.95±8.25% (negative control); 23.28±9.31%* (EF-KP); 22.98±10.39%* (positive control). It also reduces the relative percentages of CD68+IL-6+ macrophages but not significantly (p>0.05). Finally, the outcome to the grade of joint damage was scored by using Pritzker method. The treated groups have one grade lower, and the joint spaces were narrower than the untreated group. The results show the ability of the active compounds in EF-KP, which are comparable to 0.042 mg/Kg quercetin, to inhibit the progress of lupus arthritis pathogenesis in mice. It reveals the effectiveness of the EF-KP in human with lupus arthritis. However, the further clinical research is necessary.

scholarly journals Non-Chemical Control of Charcoal Rot of Urdbean by Sonchus oleraceous Application

2020 ◽  
Vol 38 ◽  
Author(s):  
S. BANARAS ◽  
A. JAVAID ◽  
A. SHOAIB
Keyword(s):  
Grain Yield ◽  
Sandy Loam ◽  
Dry Weight ◽  
Positive Control ◽  
Negative Control ◽  
Growth And Yield ◽  
Charcoal Rot ◽  
Shoot Dry Weight ◽  
Dry Biomass ◽  
Different Doses

ABSTRACT: Urdbean [Vigna mungo (L.) Hepper] is an important leguminous crop whose production is severely affected by charcoal rot disease caused by Macrophomina phaseolina. This study was undertaken to seek an environmental friendly alternative to synthetic fungicides for management of this disease. Dry biomass of an allelopathic weed Sonchus oleraceous L. was used to combat the menace. Fumigated sandy loam pot soil, pre-inoculated with M. phaseolina, was amended with different doses of dry biomass of the weed ranging from 0.5% to 3%. Application of 2% weed biomass completely controlled the disease. Soil inoculation with M. phaseolina inoculation (positive control) reduced shoot dry weight and grain yield of urdbean by 59% and 91%, respectively, over negative control. Application of different doses of soil amendment in pathogen inoculated soil enhanced shoot dry weight and grain yield of urdbean by 107-307% and 438-7400%, respectively, over positive control. The highest positive effect on growth and yield of uedbean in M. phaseolina contaminated soil was recorded due to 2.5% amendment. M. phaseolina inoculation significantly enhanced peroxidase (POX) and phenyl alanine ammonia lyase (PAL) activities. However, application of different doses of S. oleraceous biomass to the soil gradually decreased activities of these enzymes. The present study concludes that application of 2.5% dry biomass of S. oleraceous can completely control charcoal rot of urdbean and significantly enhance crop growth and yield.

scholarly journals The Potential of Billygoat Weed Leaf Extract (Ageratum Conyzoides) in Increasing Platelet Counts of Male Mice (Mus Musculus)

2020 ◽  
Vol 11 (1) ◽  
pp. 51-57
Author(s):  
Yessy Velina ◽  
Nirva Diana ◽  
Aulia Annisa
Keyword(s):  
Platelet Count ◽  
Positive Control ◽  
Negative Control ◽  
Plain Water ◽  
P Value ◽  
Male Mice ◽  
Ageratum Conyzoides ◽  
Platelet Counts ◽  
Randomized Design ◽  
Significant Difference

Billygoat weed (Ageratum conyzoides) is mainly used to treat wounds and fever. People also do not understand the use of these leaves in the treatment to increase the platelet counts. The objectives of this research Objectives were to test whether the Billygoat Weed’s leaves can increase platelet count of male mice and to find out what is the most effective dose of the Billygoat Weed on the platelet count of male mice. This research was done by employing the true experimental approach with the completely randomized design which consisted of 5 treatments, namely the negative control (plain water), the positive control (acetylsalicylic acid), 1.68 mg/kgBW, 2.52 mg/kgBW, and 3.36 mg/kgBW on five mice in each treatment. The increase in the platelet counts, based on the results of One-Way ANOVA with a p-value of 0,0004, the Billygoat Weed extract was able to increase the platelet counts. Through further testing, the smallest significant difference showed that a dose of 3.36 mg/kgBW was effectively able to increase the platelet counts of male mice with an average of 11140.80/. Billygoat weed (Ageratum conyzoides) is mainly used to treat wounds and fever. People also do not understand the use of these leaves in the treatment to increase the platelet counts. The objectives of this research Objectives were to test whether the Billygoat Weed’s leaves can increase platelet count of male mice and to find out what is the most effective dose of the Billygoat Weed on the platelet count of male mice. This research was done by employing the true experimental approach with the completely randomized design which consisted of 5 treatments, namely the negative control (plain water), the positive control (acetylsalicylic acid), 1.68 mg/kgBW, 2.52 mg/kgBW, and 3.36 mg/kgBW on five mice in each treatment. The increase in the platelet counts, based on the results of One-Way ANOVA with a p-value of 0,0004, the Billygoat Weed extract was able to increase the platelet counts. Through further testing, the smallest significant difference showed that a dose of 3.36 mg/kgBW was effectively able to increase the platelet counts of male mice with an average of 11140.80/𝑚𝑚3.

scholarly journals Use of Bamboo Vinegar as an Alternative to Control Downy Mildew in Maize

2020 ◽  
Vol 3 (2) ◽  
pp. 17-24
Author(s):  
Muhammad Habibullah ◽  
Danes Suhendra
Keyword(s):  
Downy Mildew ◽  
Disease Severity ◽  
Disease Incidence ◽  
Leaf Tissue ◽  
Dry Weight ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Resistant Strains ◽  
Control Application

Introduction: Downy mildew control using pesticides has been used intensively and led to the onset of resistant strains. This research aims to find out the effect of bamboo vinegar administration on disease incidence, disease severity, disease progression, and growth of maize. Materials and Methods: This research was conducted from June to August 2020 in the laboratory and Screenhouse of the Faculty of Agriculture, Universitas Tidar. The experiment was experimentally conducted using a Completely Randomized Design (CRD) consisting of 3 treatments with 6. are plants. In each polybag used there are 3 maize so there are 54 plants tested. The treatment tested is of bamboo vinegar and maydis (negative control), of bamboo vinegar with inoculation P. maydis (positive control), application bamboo vinegar 2 ml l-1 with inoculation P. maydis. Observations on the agronomic influence of maize covering height, , and dry weight were analyzed using ANOVA at a significance of 5% followed by LSD test at the same real level using software R v. 4.0.2. Results: Bamboo vinegar showed lignification in the treatment of stronger bamboo vinegar in corn leaf tissue, did not decrease in disease incidence, but decreased the disease severity by 22.18% and decreased disease the progression by 34.74% based on AUDPC value. The growth of maize, bamboo vinegar-treated shows values are not significant negative control.

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