Arbuscular Mycorrhizal Fungus Improves Rhizobium–Glycyrrhiza Seedling Symbiosis under Drought Stress

Rhizobia and arbuscular mycorrhizal (AM) fungi can potentially alleviate the abiotic stress on the legume Glycyrrhiza (licorice), while the potential benefits these symbiotic microbes offer to their host plant are strongly influenced by environmental factors. A greenhouse pot experiment was conducted to investigate the effects of single and combined inoculation with a rhizobium Mesorhizobium tianshanense Chen and an AM fungus Rhizophagus irregularis Walker & Schuessler on Glycyrrhiza uralensis Fisch. seedling performance under different water regimes. Drought stress inhibited rhizobium nodulation but increased mycorrhizal colonization. Furthermore, co-inoculation of rhizobium and AM fungus favored nodulation under both well-watered and drought stress conditions. Glycyrrhiza seedling growth showed a high mycorrhizal dependency. The seedlings showed a negative growth dependency to rhizobium under well-watered conditions but showed a positive response under drought stress. R. irregularis-inoculated plants showed a much higher stress tolerance index (STI) value than M. tianshanense-inoculated plants. STI value was more pronounced when plants were co-inoculated with R. irregularis and M. tianshanense compared with single-inoculated plants. Plant nitrogen concentration and contents were significantly influenced by inoculation treatments and water regimes. R. irregularis inoculation significantly increased plant shoot and root phosphorus contents. AM fungus inoculation could improve Glycyrrhiza plant–rhizobium symbiosis under drought stress, thereby suggesting that tripartite symbiotic relationships were more effective for promoting plant growth and enhancing drought tolerance.

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A differential capacity of arbuscular mycorrhizal fungal colonization under well-watered conditions and its relationship with drought stress mitigation in unimproved vs. improved soybean genotypes

Botany ◽

10.1139/cjb-2017-0137 ◽

2018 ◽

Vol 96 (2) ◽

pp. 135-144 ◽

Cited By ~ 6

Author(s):

María Soraya Salloum ◽

María Florencia Menduni ◽

Celina Mercedes Luna

Keyword(s):

Drought Stress ◽

Growth Parameters ◽

Selection Criterion ◽

Arbuscular Mycorrhizal ◽

Field Capacity ◽

Am Fungi ◽

Mineral Nutrient ◽

Fungal Colonization ◽

Mycorrhizal Dependency ◽

Soybean Genotypes

Modern breeding programs may cause a reduction in plant responsiveness to arbuscular mycorrhizal (AM) fungi. In this study, we tested the hypothesis that responses such as higher arbuscule formation and mycorrhizal dependency (MD) in unimproved soybean genotypes than in improved genotypes is related to drought stress tolerance caused by enhanced growth parameters and oxidative stress regulation. Firstly, four unimproved and four improved soybean genotypes were compared under well-watered conditions. After 20 days, all of the unimproved soybean genotypes showed increased arbuscule formation, as well as a positive and higher MD index in foliar mineral nutrient and growth parameters compared with the four improved genotypes. Secondly, tolerance to drought stress was evaluated in the two improved soybean genotypes and the two unimproved genotypes selected for the most contrasting response to arbuscule formation under well-watered conditions. After 20 days of 30% of field capacity, arbuscule formation was higher in the unimproved than improved genotypes. Mycorrhizal dependency evaluated as leaf area as well as shoot and root dry mass were highest in the unimproved AM genotypes. Moreover, levels of malondiadehide were lower and proline was higher in the unimproved rather than the improved genotypes. The potential capacity of arbuscule formation is discussed as a selection criterion to identify improved soybean genotypes with increased efficiency under well-watered conditions and an enhanced capacity to relieve drought stress.

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Arbuscular mycorrhizal fungus influence maize root growth and architecture in rock phosphate amended tropical soil

Anales de Biología ◽

10.6018/analesbio.39.22 ◽

2017 ◽

pp. 211-222 ◽

Cited By ~ 3

Author(s):

Perumalsamy Priyadharsini ◽

Thangavelu Muthukumar

Keyword(s):

Nutrient Uptake ◽

Rock Phosphate ◽

Arbuscular Mycorrhizal Fungus ◽

Mycorrhizal Fungus ◽

Arbuscular Mycorrhizal ◽

Chemical Fertilizers ◽

Mycorrhizal Dependency ◽

Acid And Alkaline Phosphatase ◽

Am Fungus ◽

Scutellospora Calospora

Hemos ensayado la influencia del homgo micorrícizo arbuscular (AM) Scutellospora calospora en la estructura, crecimiento, asimilación de nutrientes, actividad fosfatasa y dependencia micorrizal de raíces de maíz por adicción de 0-5% de fosforita (RP) en suelos deficientes de fósforo (P). La adicción de RP aumentó significativamente la longitud total de la raíz, el número de raíces a diferentes niveles y el diámetro de los pelos radiculares de las plantas AM. El hongo AM influyó positivamente el crecimiento del maíz y la asimilación de nutrientes. Las actividades fosfatasa ácida y alcalina fueron mayores en las plantas AM en suelos mejorados. Al aumentar las concentraciones RP se redujeron no linealmente el porcentaje de colonización del hongo AM. Entonces, la inoculación de hongos AM junto a la mejora de fósfoso proveniente de RP podría sustituir fertilizantes químicos y hacer disponible el P proveniente de RP. We evaluated the influence of arbuscular mycorrhizal (AM) fungus Scutellospora calospora on root architecture, growth, nutrient uptake, root phosphatase activity and mycorrhizal dependency of maize in 0-5% rock phosphate (RP) amended phosphorus (P) deficient soil. RP amendment significantly increased total root length, number of roots in different orders, and root hair diameter of AM plants. The AM fungus positively influenced maize growth and nutrient uptake. Acid and alkaline phosphatase activities were higher for AM plants in RP amended soils. In contrast, increasing concentrations of RP reduced the percentage of AM fungus colonization non-linearly. Thus, AM fungus inoculation along with RP amendment could substitute chemical fertilizers and make available the P in RP to the plants.

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Microkamienskia gen. nov. and Microkamienskia peruviana, a new arbuscular mycorrhizal fungus from Western Amazonia

Nova Hedwigia ◽

10.1127/nova_hedwigia/2019/0551 ◽

2019 ◽

Vol 109 (3) ◽

pp. 355-368 ◽

Cited By ~ 3

Author(s):

Mike Anderson Corazon-Guivin ◽

Agustin Cerna-Mendoza ◽

Juan Carlos Guerrero-Abad ◽

Adela Vallejos-Tapullima ◽

Santos Carballar-Hernández ◽

...

Keyword(s):

Mycorrhizal Fungi ◽

Mycorrhizal Fungus ◽

Arbuscular Mycorrhizal ◽

Am Fungi ◽

Single Species ◽

Spore Wall ◽

Wall Layer ◽

Am Fungus ◽

Small Spore ◽

Plukenetia Volubilis

A new arbuscular mycorrhizal (AM) fungus, Microkamienskia peruviana, was detected in bait cultures for arbuscular mycorrhizal fungi established with rhizospheric soil substrates of the inka nut (Plukenetia volubilis). The field soil derived from three agricultural plantations in the Amazonia lowlands of the province Lamas, San Martin State, in Peru. The fungus was subsequently propagated in single species cultures on Sorghum sp., Brachiaria sp.,Medicago sativa and P. volubilis as host plants. The new species differentiates hyaline spores regularly in spore clusters, up to 500–800×400–600 μm. The spores are 16–31(–36)×13–29(–35) μm in diam, formed on cylindrical or slightly funnel-shaped hyphae, without a septum at or close to the spore base. Phylogenetically, the new fungus belongs to a new genus, named Microkamienskia, which has as type species M. perpusilla comb. nov. and to which also M. divaricata comb. nov. belongs. Both are transferred from Kamienskia to Microkamienskia in the present study. The new fungus can be identified by the ballooning semi-persistent to evanescent outer spore wall layer in PVLG-based mountants that is not known for the other species of these two genera, nor for any other glomeromycotan species of similar small spore sizes. Kamienskia and Microkamienskia species can be distinguished by their position in the phylogenetic tree and by hyaline spores, open pores at the spore bases and in the subtending hyphae, and by their spore sizes that are for Microkamienskia among the smallest spore sizes so far detected for AM fungi (15–35 μm).

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Transgenic Bt cotton inhibited arbuscular mycorrhizal fungus differentiation and colonization

Plant Soil and Environment ◽

10.17221/743/2016-pse ◽

2017 ◽

Vol 63 (No. 2) ◽

pp. 62-69 ◽

Cited By ~ 1

Author(s):

Chen Xiuhua ◽

Zhang Rui ◽

Wang Fengling

Keyword(s):

Mycorrhizal Fungus ◽

Arbuscular Mycorrhizal ◽

Am Fungi ◽

Hyphal Growth ◽

Bt Cotton ◽

Signal Perception ◽

Bt Toxins ◽

Am Fungus ◽

The Impact ◽

Direct Toxicity

The present study investigated the impact of transgenic Bacillus thuringiensis (Bt) cotton on several aspects of arbuscular mycorrhizal (AM) fungus Funneliformis mosseae. The results showed that Bt cotton significantly inhibited spore germination and pre-symbiotic hyphal growth. The appressorium density, arbuscule frequency and colonization intensity in Bt roots were also decreased. The statistical analysis demonstrated that the transformation event resulted in the inhibition of hyphal development and colonization. The reduced interaction between AM fungi and plants could affect nutrient uptake and transportation in plant-fungus symbiosis. The mechanism might involve the direct toxicity of Bt toxins or the interference of signal perception between AM fungus and Bt cotton.

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Target of rapamycin, PvTOR, is a key regulator of arbuscule development during mycorrhizal symbiosis in Phaseolus

Scientific Reports ◽

10.1038/s41598-021-90288-2 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Manoj-Kumar Arthikala ◽

Kalpana Nanjareddy ◽

Lourdes Blanco ◽

Xóchitl Alvarado-Affantranger ◽

Miguel Lara

Keyword(s):

Arbuscular Mycorrhizal ◽

Am Fungi ◽

Target Of Rapamycin ◽

Mycorrhizal Symbiosis ◽

Energy Status ◽

Symbiotic Associations ◽

Expression Of Genes ◽

Fungal Symbiosis ◽

Am Fungus ◽

Am Symbiosis

AbstractTarget of rapamycin (TOR) is a conserved central growth regulator in eukaryotes that has a key role in maintaining cellular nutrient and energy status. Arbuscular mycorrhizal (AM) fungi are mutualistic symbionts that assist the plant in increasing nutrient absorption from the rhizosphere. However, the role of legume TOR in AM fungal symbiosis development has not been investigated. In this study, we examined the function of legume TOR in the development and formation of AM fungal symbiosis. RNA-interference-mediated knockdown of TOR transcripts in common bean (Phaseolus vulgaris) hairy roots notably suppressed AM fungus-induced lateral root formation by altering the expression of root meristem regulatory genes, i.e., UPB1, RGFs, and sulfur assimilation and S-phase genes. Mycorrhized PvTOR-knockdown roots had significantly more extraradical hyphae and hyphopodia than the control (empty vector) roots. Strong promoter activity of PvTOR was observed at the site of hyphal penetration and colonization. Colonization along the root length was affected in mycorrhized PvTOR-knockdown roots and the arbuscules were stunted. Furthermore, the expression of genes induced by AM symbiosis such as SWEET1, VPY, VAMP713, and STR was repressed under mycorrhized conditions in PvTOR-knockdown roots. Based on these observations, we conclude that PvTOR is a key player in regulating arbuscule development during AM symbiosis in P. vulgaris. These results provide insight into legume TOR as a potential regulatory factor influencing the symbiotic associations of P. vulgaris and other legumes.

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Application of Mycorrhizal Technology for Improving Yield Production of Common Bean Plants

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v4i2.15103 ◽

2016 ◽

Vol 4 (2) ◽

pp. 191-197 ◽

Cited By ~ 7

Author(s):

Gamal M. Abdel-Fattah ◽

Wafaa M. Shukry ◽

Mahmoud M.B. Shokr ◽

Mai A. Ahmed

Keyword(s):

Common Bean ◽

Arbuscular Mycorrhizal ◽

Am Fungi ◽

Growth And Yield ◽

Mycorrhizal Dependency ◽

Npk Fertilizers ◽

Yield Production ◽

Bean Plants ◽

Mycorrhizal Plants ◽

Common Bean Plants

This study aimed to investigate the effects of arbuscular mycorrhizal (AM) fungi with different levels of NPK fertilizers on yield production of common bean plants which common bean plants were subjected to five levels of NPK fertilizers (0, 25, 50, 75, 100 %). Application of AMF significantly increased the growth and yield components of common beans with minimized the levels of NPK comparing to equivalents non-mycorrhizal ones. The results obtained revealed that inoculation with AMF and the concentrations 50% and 75% of NPK with AMF are the greater than other concentrations and non-mycorrhizal plants. Mycorrhizal Common bean plants had significantly higher number of pods, length of one pod, pods weight, 100 seeds weight, weight of seed/plant and intensity of mycorrhizal colonization(M%) . Concentrations of nutrients (N, P, K, Ca and Mg) and total carbohydrates, crude protein and mycorrhizal dependency of some yield parameters were significantly increased in mycorrhizal plants at different NPK levels when comparing to those of non-mycorrhizal plants paticularly at (50% and 75%) concentration of NPK, but lower Na concentration in mycorrhizal common bean seeds than those of non-mycorrhizal.Int J Appl Sci Biotechnol, Vol 4(2): 191-197

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Frequent cultivation prior to planting to prevent weed competition results in an opportunity for the use of arbuscular mycorrhizal fungus inoculum

Renewable Agriculture and Food Systems ◽

10.1017/s1742170511000391 ◽

2011 ◽

Vol 27 (4) ◽

pp. 251-255 ◽

Cited By ~ 5

Author(s):

David D. Douds ◽

Gerald Nagahashi ◽

John E. Shenk

Keyword(s):

Management Practices ◽

Mycorrhizal Fungus ◽

Arbuscular Mycorrhizal ◽

Am Fungi ◽

Indigenous Populations ◽

Paspalum Notatum ◽

Allium Porrum ◽

Potential Yield ◽

Greenhouse Production ◽

Synthetic Chemicals

AbstractInoculation with arbuscular mycorrhizal (AM) fungi is a potentially useful tool in agricultural systems with limited options regarding use of synthetic chemicals for fertility and pest control. We tested the response ofAllium porrumcv. Lancelot to inoculation with AM fungi in a field high in available P (169 μg g−1soil) that had been repeatedly cultivated to control weeds. Seedlings were inoculated during the greenhouse production period with a mixed species inoculum produced on-farm in a compost and vermiculite medium withPaspalum notatumFlugge as a nurse host. Inoculated and uninoculated seedlings were the same size at outplanting. Inoculated seedlings were over 2.5-fold greater in shoot weight and shoot P content than uninoculated seedlings at harvest. These results demonstrate the potential yield benefits from inoculation with AM fungi in situations where farm management practices may negatively impact on indigenous populations of AM fungi.

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Biocontrol of Sclerotium rolfsii in Groundnut by Using Microbial Inoculants

Notulae Scientia Biologicae ◽

10.15835/nsb919992 ◽

2017 ◽

Vol 9 (1) ◽

pp. 124-130 ◽

Cited By ~ 6

Author(s):

Khirood DOLEY ◽

Mayura DUDHANE ◽

Mahesh BORDE

Keyword(s):

Mycorrhizal Fungi ◽

Antioxidant Activities ◽

Sclerotium Rolfsii ◽

Arbuscular Mycorrhizal ◽

Am Fungi ◽

Stem Rot ◽

Mycorrhizal Dependency ◽

Trichoderma Species ◽

Arachis Hypogaea L ◽

Microbial Strains

Sclerotium rolfsii (Sacc.) is the causal agent of stem-rot in groundnut (Arachis hypogaea L.)crop. With the increase in demand for the groundnut, control of stem-rot efficiently by microbial strains is fast becoming inevitable as the conventional system of chemicals is degrading our ecosystem. This investigation here emphasizes on inoculation of arbuscular mycorrhizal fungi (AMF) and Trichoderma species for growth achievement and disease control. The present investigation showed that these microbial strains were found to be worth applying as they stimulated growth and decreased harmful effects of S. rolfsii (cv. ‘Western-51’). The increased biochemical parameters and antioxidant activities also indicated their defence related activities in groundnut plants. In spite of positive attributes meted out by these microbial strains towards groundnut crop, the interaction among AM fungi and Trichoderma species seemed to be less co-operative between each other which were noted when mycorrhizal dependency and percent root colonization were observed. However, in summary more practical application of low-input AM fungi along with Trichoderma species may be needed for the advancement of modern agricultural systems.

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Elucidating the Possible Involvement of Maize Aquaporins in the Plant Boron Transport and Homeostasis Mediated by Rhizophagus irregularis under Drought Stress Conditions

International Journal of Molecular Sciences ◽

10.3390/ijms21051748 ◽

2020 ◽

Vol 21 (5) ◽

pp. 1748

Author(s):

Gabriela Quiroga ◽

Gorka Erice ◽

Ricardo Aroca ◽

Juan Manuel Ruiz-Lozano

Keyword(s):

Drought Stress ◽

Higher Plants ◽

Mycorrhizal Fungus ◽

Membrane Integrity ◽

Arbuscular Mycorrhizal ◽

Transport Processes ◽

Stress Conditions ◽

Facilitated Diffusion ◽

Growing Medium ◽

Am Symbiosis

Boron (B) is an essential micronutrient for higher plants, having structural roles in primary cell walls, but also other functions in cell division, membrane integrity, pollen germination or metabolism. Both high and low B levels negatively impact crop performance. Thus, plants need to maintain B concentration in their tissues within a narrow range by regulating transport processes. Both active transport and protein-facilitated diffusion through aquaporins have been demonstrated. This study aimed at elucidating the possible involvement of some plant aquaporins, which can potentially transport B and are regulated by the arbuscular mycorrhizal (AM) symbiosis in the plant B homeostasis. Thus, AM and non-AM plants were cultivated under 0, 25 or 100 μM B in the growing medium and subjected or not subjected to drought stress. The accumulation of B in plant tissues and the regulation of plant aquaporins and other B transporters were analyzed. The benefits of AM inoculation on plant growth (especially under drought stress) were similar under the three B concentrations assayed. The tissue B accumulation increased with B availability in the growing medium, especially under drought stress conditions. Several maize aquaporins were regulated under low or high B concentrations, mainly in non-AM plants. However, the general down-regulation of aquaporins and B transporters in AM plants suggests that, when the mycorrhizal fungus is present, other mechanisms contribute to B homeostasis, probably related to the enhancement of water transport, which would concomitantly increase the passive transport of this micronutrient.

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Improved tolerance of wheat plants (Triticum aestivum L.) to drought stress and rewatering by the arbuscular mycorrhizal fungus Glomus claroideum: effect on growth and cell membrane stability

Brazilian Journal of Plant Physiology ◽

10.1590/s1677-04202008000100004 ◽

2008 ◽

Vol 20 (1) ◽

pp. 29-37 ◽

Cited By ~ 55

Author(s):

José Beltrano ◽

Marta G. Ronco

Keyword(s):

Water Stress ◽

Drought Stress ◽

Mycorrhizal Fungus ◽

Arbuscular Mycorrhizal ◽

Dry Weight ◽

Leaf Chlorophyll ◽

Severe Water Stress ◽

Relative Water ◽

Mycorrhizal Plants ◽

Total Dry Weight

The aim of this paper was to investigate the contribution of the arbuscular mycorrhizal fungus Glomus claroideum to drought stress tolerance in wheat plants grown under controlled conditions in a growth chamber, and subjected to moderate or severe water stress and rewatering. Water stress tolerance was determined through total dry weight, leaf relative water content, leakage of solutes and leaf chlorophyll and protein concentrations in mycorrhizal and non-mycorrhizal wheat plants. Total dry weight and leaf chlorophyll concentrations were significantly higher in mycorrhizal plants after moderate or severe water stress treatments compared with non-mycorrhizal ones. Electrolyte leakage was significantly lower in water-stressed inoculated plants. Compared to non-inoculated plants, leaf relative water content and total protein concentration of inoculated individuals increased only under severe water stress. When irrigation was re-established, mycorrhizal plants increased their total dry weight and leaf chlorophyll concentration, and recovered cell membrane permeability in leaves compared with non-mycorrhizal plants. In conclusion, root colonization by G. claroideum could be an adequate strategy to alleviate the deleterious effects of drought stress and retard the senescence syndrome in wheat.

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