scholarly journals Flavonoid-Rich Extract of Paeonia lactiflora Petals Alleviate d-Galactose-Induced Oxidative Stress and Restore Gut Microbiota in ICR Mice

Antioxidants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1889
Author(s):  
Lei Liu ◽  
Yingdan Yuan ◽  
Jun Tao
Keyword(s):  
Oxidative Stress ◽  
Gut Microbiota ◽  
Epigallocatechin Gallate ◽  
Experimental Period ◽  
High Dose ◽  
Intragastric Administration ◽  
Paeonia Lactiflora ◽  
Icr Mice ◽  
Stress Markers ◽  
Dependent Relationship

This study was aimed to investigate the antioxidant effect of Paeonia lactiflora Pall. petal flavonoids extract (PPF) on d-galactose (d-gal)-induced ICR mice. In this study, sixty male ICR mice were randomly divided into six groups during an 8 weeks experimental period, including normal control (NC) group, d-gal group, epigallocatechin gallate (EGCG) group, low, medium, and high dose PPF groups (10, 20 and 40 mg/kg/day). The results showed that intragastric administration with PPF significantly reverses the atrophy of the visceral organs of oxidative damage mice in a dose-dependent relationship. PPF indicated the antioxidant capacity to decrease the malondialdehyde (MDA) level and improve the activity of superoxide dismutase (SOD), catalase (CAT) as well as glutathione peroxidase (GSH-Px). In addition, PPF treatment reversed gut microbiota dysbiosis by increasing the relative abundance of Lactobacillaceae. Spearman correlation analysis showed that the body’s oxidative stress markers were directly related to changes in gut microbiota. These findings reveal firstly that PPF could alleviate d-Gal-induced oxidative stress and modulate gut microbiota balance.

scholarly journals ANTIOXIDATIVE EFFECT OF EPIGALLOCATECHIN GALLATE AGAINST D-GALACTOSAMINE-INDUCED INJURY IN PRIMARY CULTURE OF RAT HEPATOCYTES

2014 ◽  
Vol 57 (1) ◽  
pp. 3-8 ◽  
Author(s):  
Alena Moravcová ◽  
Zuzana Červinková ◽  
Otto Kučera ◽  
Vojtěch Mezera ◽  
Halka Lotková
Keyword(s):  
Oxidative Stress ◽  
Primary Culture ◽  
Green Tea ◽  
Epigallocatechin Gallate ◽  
Preventive Treatment ◽  
Rat Hepatocytes ◽  
High Dose ◽  
Antioxidative Effect ◽  
Lactate Dehydrogenase Activity ◽  
Albumin Production

Literature data support that green tea and its major component epigallocatechin gallate (EGCG) have powerful antioxidant effects. Contrary, hepatotoxicity can be induced by high-dose EGCG. The timing of exposure to green tea in relation to administration of hepatotoxic agent plays an import role too. The aim of our work was a verification of antioxidative effect of EGCG on D-galactosamine-induced injury in primary culture of rat hepatocytes. Hepatocytes were incubated with EGCG at concentrations of 1.25–10 μM and toxic D-galactosamine (GalN) for 24 hrs. Alternatively, hepatocytes were pretreated with EGCG for 24 hrs, and then incubated with EGCG and GalN for further 24 hrs. Cytotoxicity was analysed by lactate dehydrogenase activity, functional capacity by albumin production. Oxidative stress was evaluated from a production of malondialdehyde and glutathione content in the cells. EGCG protected hepatocytes against GalN-induced cytotoxicity but preventive treatment of intact hepatocytes with EGCG was required to diminish the development of hepatocyte injury. Oxidative stress induced in our study seems to overcome the ability of hepatocytes to improve GSH depletion and albumin production. Prolongation of the pretreatment with EGCG could be a promising strategy leading to amelioration of its hepatoprotective effect.

scholarly journals The Effect of High-Dose Insulin Analog Initiation Therapy on Lipid Peroxidation Products and Oxidative Stress Markers in Type 2 Diabetic Patients

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Hazal Tuzcu ◽  
Ibrahim Aslan ◽  
Mutay Aslan
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Blood Glucose ◽  
Glucose Variability ◽  
Insulin Analog ◽  
High Dose ◽  
Oxidative Stress Markers ◽  
Insulin Analogs ◽  
Stress Markers ◽  
Mean Blood Glucose

Effect of high-dose insulin analog initiation therapy was evaluated on lipid peroxidation and oxidative stress markers in type 2 diabetes mellitus (T2DM). Twenty-four T2DM patients with HbA1c levels above 10% despite ongoing therapy with sulphonylurea and metformin were selected. Former treatment regimen was continued for the first day followed by substitution of sulphonylurea therapy with different insulin analogs. Glycemic profiles were determined over 72 hours by Continuous Glucose Monitoring System (CGMS), and blood/urine samples were collected at 24 and 72 hours. Insulin analog plus metformin treatment significantly reduced glucose variability. Plasma and urine lipid peroxidation were markedly decreased following insulin analog plus metformin treatment. No correlation existed between glucose variability and levels of plasma and urine oxidative stress markers. Likewise, changes in mean blood glucose from baseline to end point showed no significant correlation with changes in markers of oxidative stress. On the contrary, decreased levels of oxidative stress markers following treatment with insulin analogs were significantly correlated with mean blood glucose levels. In conclusion, insulin plus metformin resulted in a significant reduction in oxidative stress markers compared with oral hypoglycemic agents alone. Data from this study suggests that insulin analogs irrespective of changes in blood glucose exert inhibitory effects on free radical formation.

scholarly journals The effect of short-term, high-dose oral N-acetylcysteine treatment on oxidative stress markers in cystic fibrosis patients with chronic P. aeruginosa infection — A pilot study

2015 ◽  
Vol 14 (2) ◽  
pp. 211-218 ◽  
Author(s):  
Marianne Skov ◽  
Tacjana Pressler ◽  
Jens Lykkesfeldt ◽  
Henrik Enghusen Poulsen ◽  
Peter Østrup Jensen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cystic Fibrosis ◽  
Pilot Study ◽  
High Dose ◽  
Oxidative Stress Markers ◽  
Short Term ◽  
Stress Markers ◽  
Dose Oral

Protective role of garlic against malathion induced oxidative stress in male albino rats

2016 ◽  
Vol 50 (3) ◽  
Author(s):  
Gamila A. M. Kotb ◽  
Farag A.A. Gh ◽  
Kholoud S Ramadan ◽  
Hoda E.A. Farid
Keyword(s):  
Oxidative Stress ◽  
Ache Activity ◽  
Protein Pattern ◽  
Experimental Period ◽  
Protective Role ◽  
Oxidative Stress Marker ◽  
Albino Rats ◽  
Stress Markers ◽  
Therapeutic Properties

The garlic has been widely used as medicinal plant for its therapeutic properties This study was aimed to investigate the antioxidant role of garlic (G) against oxidative stress induced by malathion (M) in male albino rats. After experimental period (28 days), the study investigated some biochemical parameters and oxidative stress markers in plasma rats. The results revealed that, malathion induced significant increase in plasma Tri-iodothyronine (T<sub>3</sub>), Thyroxin (T<sub>4</sub>), glucose values and malondialdehyde (MDA) as oxidative stress marker was noticed. However, significant decrease was recorded in cholesterol, total protein (T. Protein) contents and in defense system biomarker total SH- protein. Acetylcholinesterase (AChE) activity was inhibited by malathion treatment and cause alteration in non-specific esterase and protein pattern. Finally, these results concluded that garlic has significant protection against malathion intoxication demonstrated inhibition in acetyl cholinesterase (AChE) activity and reduced in cholesterol, T. protein and total SH- protein. Further studies are necessary to investigate the significant effect of garlic on thyroid gland, brain and neurotransmitters.

The anti-oxidative Effect of Chrysanthemum Extract on Retinal- light Damage of Mice

2020 ◽  
Author(s):  
YiBo Gong ◽  
Xuechun Wang ◽  
Yuchuan Wang ◽  
Peng Hao ◽  
Yatu Guo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Age Related Macular Degeneration ◽  
High Dose ◽  
Intragastric Administration ◽  
Ros Production ◽  
Light Damage ◽  
High Concentration ◽  
Retinal Light Damage ◽  
Age Related

Abstract Background:Apoptosis of photoreceptor cells and oxidative stress of RPE in age-related macular degeneration (AMD) could be promoted by photopic oxidative stress. In our study we are aim to study the protective effect of chrysanthemum extract on light damaged retina of mice.Methods:In vitro, ARPE-19 cells are incubated and divided into four groups: the control, the light damaged, the low and high dose-chrysanthemum extract groups. The last three groups were dropped in zero, low and high concentration of chrysanthemum extract separately before exposing to light. Cellular viability and Reactive Oxygen Species(ROS) production were measured by MTT and immunofluorescence. In vivo, C57BL/6J mice were divided into four groups as above mentioned. Low and high concentration of chrysanthemum extract were given by continuous intragastric administration before being exposed to white light. Retinal function was evaluated by electroretinogram. Optical coherence tomography and Fluorescein fundus angiography were used to observe the morphology and vessels. HE staining and TUNEL immunofluorescence for presenting morphology and apoptosis of isolated retina.Results:Viability of ARPE-19 cells decreased and ROS production increased after the light damaged. However, treatment with chrysanthemum extract, viability improved and ROS declined. After light injury, dysfunctional retina, destroyed morphology and increased apoptosis rate were observed in mice especially in RPE and photoreceptor layer. Treatment with chrysanthemum extract, retina function improved as well as structure of RPE and photoreceptor layers. Rate of apoptosis decreased via the raised concentration of anti-oxidative enzyme superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). Conclusions:Preventive administration of chrysanthemum extract reduces the oxidative-stress induced by light damage, which indicating Chrysanthemum have a potential of preventive measure for AMD.

scholarly journals Diosmin Mitigates Cyclophosphamide Induced Premature Ovarian Insufficiency in Rat Model

2021 ◽  
Vol 22 (6) ◽  
pp. 3044
Author(s):  
Noha M. Abogresha ◽  
Sally S. Mohammed ◽  
Marwa M. Hosny ◽  
Hoda Y. Abdallah ◽  
Ahmed M. Gadallah ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Target Genes ◽  
Histopathological Examination ◽  
Protective Role ◽  
Premature Ovarian Insufficiency ◽  
Albino Rats ◽  
High Dose ◽  
Ovarian Insufficiency ◽  
Stress Markers ◽  
Intraperitoneal Dose

The current study was designed to investigate the protective role of diosmin against cyclophosphamide-induced premature ovarian insufficiency (POI). Female Swiss albino rats received a single intraperitoneal dose of cyclophosphamide (200 mg/kg) followed by 8 mg/kg/day for the next 15 consecutive days either alone or in combination with oral diosmin at 50 or 100 mg/kg. Histopathological examination of ovarian tissues, hormonal assays for follicle stimulating hormone (FSH), estradiol (E2), and anti-Mullerian hormone (AMH), assessment of the oxidative stress status, as well as measurement of the relative expression of miRNA-145 and its target genes [vascular endothelial growth factor B (VEGF-B) and regulator of cell cycle (RGC32)] were performed. Diosmin treatment ameliorated the levels of E2, AMH, and oxidative stress markers. Additionally, both low and high diosmin doses significantly reduced the histopathological alterations and nearly preserved the normal ovarian reserve. MiRNA-145 expression was upregulated after treatment with diosmin high dose. miRNA-145 target genes were over-expressed after both low and high diosmin administration. Based on our findings, diosmin has a dose-dependent protective effect against cyclophosphamide-induced ovarian toxicity in rats.

scholarly journals Changes in reproductive organs, semen characteristics and intra-testicular oxidative stress in adult male rats caused by azithromycin

2017 ◽  
Vol 5 (2) ◽  
pp. 72 ◽  
Author(s):  
Mossad El-Sayed ◽  
Mohamed Kandiel ◽  
Dalia Ebied
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Cells ◽  
Experimental Period ◽  
Reproductive Organs ◽  
Post Treatment ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Albino Rats ◽  
Stress Markers ◽  
Vascular Congestion

This study aimed to evaluate the numerous azithromycin (as a member of macrolides) effects on the male reproductive organs, spermio-gram, testicular oxidative stress markers of adults’ male albino rats. Azithromycin was administered orally once daily to male rats (200-250 b.wt.) at a dose of 45 mg (therapeutic) or 90 mg/kg b.wt. (double-therapeutic) for three or six days and scarified at the first, thirty and sixty days after the last dose of administration. A significant decrease as the index weight of the reproductive organs as well as sperm motility, livability and cell concentration, but sperm abnormalities increased at varying times post-treatment with azithromycin administration. Testosterone hormone level did not vary significantly after azithromycin dosing for three days along the experimental period. However, it differed at the first day after the end of azithromycin dosing for six days. The intra-testicular oxidative stress alteration mostly occurred at the thirty-day post-treatment in the three- and six-days protocols. In the three-days protocol, there was a significant decrease in malondialdehyde level and superoxide dismutase enzyme activity in a double-therapeutic group. In the six-days regimen, there was an increased activity of catalase enzyme, accompanied with a significant decrease in malondialdehyde levels as well as glutathione peroxidase enzymes. Double therapeutic dose for six days’ treatment was associated with vascular congestion and perivascular inflammatory cells and ho-mogenous eosinophilic material infiltration into the stroma of testes. The lumen of seminiferous tubules and epididymis showed azoo-spermia. From these results, it could be concluded that azithromycin administration has hazard effects on male adult’s rats’ fertility governed with the spermiogram, oxidative stress and the histopathological alternations during the post-treatment period.

scholarly journals Effect of Tamarindus indica juice intake on some oxidative stress markers in carbon tetrachloride induced rats

2021 ◽  
Vol 14 (1) ◽  
pp. 31-37
Author(s):  
N.S. Sadi ◽  
S.M. Abubakar ◽  
A. Ibrahim ◽  
A.M. Umar ◽  
A.M. Gadanya ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Normal Control ◽  
Colorimetric Assay ◽  
Normal Control Group ◽  
Control Group ◽  
Albino Rats ◽  
High Dose ◽  
Oxidative Stress Markers ◽  
Standard Drug ◽  
Stress Markers

Tamarind tree is a multipurpose tree of which almost every part finds at least some use, either nutritional or medicinal. Due to its pleasant acidic taste and rich aroma, the pulp is widely used for domestic and industrial purpose. A study was carried out to evaluate the effect of Tamarind juice intake in CCl4 induced oxidative stress albino rats. The Proximate, antinutrient, and Phytochemical contents of tamarind juice were analyzed using standard AOAC methods while mineral contents were determined using atomic absorption spectrometry. Oxidative stress markers were also analyzed using colorimetric assay kit. The serum levels of oxidative stress markers were compared between the normal and test groups. Experimental rats were divided into five groups: Normal control group, negative control (CCl4) group, standard drug (Vitamin C) group, tamarind low and high dose group. At the end of the experiment, significant increase in malondialdehyde level and decrease in superoxide dismutase, catalase, reduced glutathione and glutathione Peroxidase activities were recorded in CCl4-exposed rats as compared to normal control group. In the tamarind supplemented groups, the level of MDA along with the activities of SOD, CAT, GSH and GPx were comparable with the normal control rats (p>0.05). Thus, it appears that tamarind juice ameliorate the effect of CCl4; suggesting that consumption of natural compounds with an antioxidant profile may be a preventive alternative to those diseases associated with oxidative stress.

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