scholarly journals Separation of Albumin from Bovine Serum Applying Ionic-Liquid-Based Aqueous Biphasic Systems

2022 ◽  
Vol 12 (2) ◽  
pp. 707
Author(s):  
Ana F. C. S. Rufino ◽  
Mafalda R. Almeida ◽  
Mukesh Sharma ◽  
João A. P. Coutinho ◽  
Mara G. Freire
Keyword(s):  
Ionic Liquid ◽  
Bovine Serum ◽  
Single Step ◽  
Added Value ◽  
Separation Performance ◽  
Serum Samples ◽  
Original Matrix ◽  
Rich Phase ◽  
Recovery Yield ◽  
Aqueous Biphasic

In this work, the extraction and separation of bovine serum albumin (BSA) from its original matrix, i.e., bovine serum, was performed using a novel ionic-liquid-based aqueous biphasic system (IL-based ABS). To this end, imidazolium-, phosphonium-, and ammonium-based ILs, combined with the anions’ acetate, arginate and derived from Good Buffers, were synthesized, characterized, and applied in the development of ABS with K2HPO4/KH2PO4 buffer aqueous solutions at pH 7. Initial studies with commercial BSA revealed a preferential migration of the protein to the IL-rich phase, with extraction efficiencies of 100% obtained in a single-step. BSA recovery yields ranging between 64.0% and 84.9% were achieved, with the system comprising the IL tetrabutylammonium acetate leading to the maximum recovery yield. With this IL, BSA was directly extracted and separated from bovine serum using the respective ABS. Different serum dilutions were further investigated to improve the separation performance. Under the best identified conditions, BSA can be extracted from bovine serum with a recovery yield of 85.6% and a purity of 61.2%. Moreover, it is shown that the BSA secondary structure is maintained in the extraction process, i.e., after being extracted to the IL-rich phase. Overall, the new ABS herein proposed may be used as an alternative platform for the purification of BSA from serum samples and can be applied to other added-value proteins.

scholarly journals One-step extraction and concentration of estrogens for an adequate monitoring of wastewater using ionic-liquid-based aqueous biphasic systems

2015 ◽  
Vol 17 (4) ◽  
pp. 2570-2579 ◽  
Author(s):  
Teresa B. V. Dinis ◽  
Helena Passos ◽  
Diana L. D. Lima ◽  
Valdemar I. Esteves ◽  
João A. P. Coutinho ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Single Step ◽  
Aqueous Biphasic Systems ◽  
One Step ◽  
Aqueous Biphasic ◽  
Biphasic Systems

Aqueous biphasic systems composed of ionic liquids allow a single-step extraction–concentration of ethinylestradiol (EE2) from wastewater up to a 1000-fold.

scholarly journals Integration of Stable Ionic Liquid-Based Nanofluids into Polymer Membranes. Part II: Gas Separation Properties toward Fluorinated Greenhouse Gases

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 582
Author(s):  
Fernando Pardo ◽  
Sergio V. Gutiérrez-Hernández ◽  
Carolina Hermida-Merino ◽  
João M. M. Araújo ◽  
Manuel M. Piñeiro ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Gas Separation ◽  
Value Added ◽  
Gas Permeation ◽  
Mixed Matrix Membranes ◽  
Separation Performance ◽  
Mixed Gas ◽  
Neat Polymer ◽  
Stable Suspension ◽  
Base Polymer

Membrane technology can play a very influential role in the separation of the constituents of HFC refrigerant gas mixtures, which usually exhibit azeotropic or near-azeotropic behavior, with the goal of promoting the reuse of value-added compounds in the manufacture of new low-global warming potential (GWP) refrigerant mixtures that abide by the current F-gases regulations. In this context, the selective recovery of difluorometane (R32, GWP = 677) from the commercial blend R410A (GWP = 1924), an equimass mixture of R32 and pentafluoroethane (R125, GWP = 3170), is sought. To that end, this work explores for the first time the separation performance of novel mixed-matrix membranes (MMMs) functionalized with ioNanofluids (IoNFs) consisting in a stable suspension of exfoliated graphene nanoplatelets (xGnP) into a fluorinated ionic liquid (FIL), 1-ethyl-3-methylpyridinium perfluorobutanesulfonate ([C2C1py][C4F9SO3]). The results show that the presence of IoNF in the MMMs significantly enhances gas permeation, yet at the expense of slightly decreasing the selectivity of the base polymer. The best results were obtained with the MMM containing 40 wt% IoNF, which led to an improved permeability of the gas of interest (PR32 = 496 barrer) with respect to that of the neat polymer (PR32 = 279 barrer) with a mixed-gas separation factor of 3.0 at the highest feed R410A pressure tested. Overall, the newly fabricated IoNF-MMMs allowed the separation of the near-azeotropic R410A mixture to recover the low-GWP R32 gas, which is of great interest for the circular economy of the refrigeration sector.

scholarly journals Effects of Operating Parameters on Ionic Liquid Membrane to Remove Humidity in a Green Continuous Process

Membranes ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 65
Author(s):  
Xueru Yan ◽  
Alexandre Favard ◽  
Stéphane Anguille ◽  
Marc Bendahan ◽  
Philippe Moulin
Keyword(s):  
Ionic Liquid ◽  
Flow Rate ◽  
Continuous Process ◽  
Hybrid Process ◽  
Absolute Pressure ◽  
Mechanical Resistance ◽  
Operating Parameters ◽  
Separation Performance ◽  
Feed Concentration ◽  
Time Operation

Membrane processes are promising methods to separate gases from feed streams without phase changing. A hybrid process, the combination of ionic liquids with a ceramic membrane (ILM), has been developed for humidity removal in a green continuous process. This new concept provides a more efficient and available ionic liquid (IL)-based membrane regeneration process, which just switches the moist feed stream to dry air. Furthermore, the ILM presents high stability and mechanical resistance during long-time operation. In addition, the influences of several operating parameters, including flow rate, temperature, absolute pressure, and feed concentration on process efficiency were investigated. The lower inlet flow rate was found to be favorable for drying humid air. Moreover, when the pressure increased, the mass of absorbed water was increased, while the feed concentration had no significant effects on the membrane separation performance. However, the operating temperature had a great effect on humidity removal. It is necessary to note that the processes at room temperature can limit the energy consumption. The absorbing process of ILM remained efficient after several absorption desorption cycles. Therefore, the new ILM hybrid process that has been developed has great potential for consecutive humidity removal processes.

Bio-Ferrography to Capture and Separate Polyethylene Wear Debris from Hip Simulator Fluid and Compared with Conventional Filter Method

2005 ◽  
Vol 128 (2) ◽  
pp. 436-441 ◽  
Author(s):  
Donna M. Meyer ◽  
Adam Tillinghast ◽  
Nevan C. Hanumara ◽  
Ana Franco
Keyword(s):  
Experimental Method ◽  
Bovine Serum ◽  
Wear Debris ◽  
Polyethylene Wear ◽  
Ultrahigh Molecular Weight Polyethylene ◽  
Filter Method ◽  
Acetabular Cup ◽  
Serum Samples ◽  
Uhmwpe Wear ◽  
Uhmwpe Particle

This paper describes an experimental method, bio-ferrography, to separate ultrahigh molecular weight polyethylene (UHMWPE) wear debris, generated in hip simulators, from bovine serum lubricating fluid. A total of 54 experiments were performed in which an enzyme digestion “cocktail” was developed and used to clean the bovine serum samples of extraneous sugars, proteins, and lipids that interfere with the UHMWPE particle separation. Erbium chloride was used to marginally magnetize particles in the fluid prior to passing through the ferrographic device. The particles were captured and separated from the fluid by traversing the treated serum across a magnetic gap of a bio-ferrograph. Morphology of the captured and separated wear debris was compared with particles from samples of fluid filtered through a paper sieve arrangement with pores of 0.05micrometers in diameter. The UHMWPE wear debris collected using the described experimental method, were found to be between 0.1 and 20micrometers in diameter with spherical and pill-shaped particles. The filtered UHMWPE particles were in the same size range as the debris separated using bio-ferrography. To show that the experimental method captured UHMWPE particles, the spectra of the chemical composition of UHMWPE from an acetabular cup insert of a hip implant and of UHMWPE particles separated using bio-ferrography were compared and found to be the same. To further demonstrate that polyethylene could be captured and separated through the experimental method, manufactured polyethylene microspheres in the diameter range of 3-45micrometers, were captured and separated using the bio-ferrographic process.

Lipase production and purification by self-buffering ionic liquid-based aqueous biphasic systems

2017 ◽  
Vol 63 ◽  
pp. 221-228 ◽  
Author(s):  
Sze Ying Lee ◽  
Ianatul Khoiroh ◽  
João A.P. Coutinho ◽  
Pau Loke Show ◽  
Sónia P.M. Ventura
Keyword(s):  
Ionic Liquid ◽  
Lipase Production ◽  
Aqueous Biphasic Systems ◽  
Aqueous Biphasic ◽  
Biphasic Systems

scholarly journals Simultaneous Detection of Drug-Induced Liver Injury Protein and microRNA Biomarkers Using Dynamic Chemical Labelling on a Luminex MAGPIX System

Analytica ◽  
2021 ◽  
Vol 2 (4) ◽  
pp. 130-139
Author(s):  
Antonio Marín-Romero ◽  
Mavys Tabraue-Chávez ◽  
Bárbara López-Longarela ◽  
Mario A. Fara ◽  
Rosario M. Sánchez-Martín ◽  
...  
Keyword(s):  
Liver Injury ◽  
Simultaneous Detection ◽  
Single Step ◽  
Qualitative And Quantitative Analysis ◽  
Serum Samples ◽  
Drug Induced ◽  
Drug Induced Liver Injury ◽  
Qualitative And Quantitative ◽  
Post Marketing ◽  
Mechanistic Basis

Drug-induced liver injury (DILI) is a potentially fatal adverse event and a leading cause for pre- and post-marketing drug withdrawal. Several multinational DILI initiatives have now recommended a panel of protein and microRNA (miRNA) biomarkers that can detect early liver injury and inform about mechanistic basis. This manuscript describes the development of seqCOMBO, a unique combo-multiplexed assay which combines the dynamic chemical labelling approach and an antibody-dependant method on the Luminex MAGPIX system. SeqCOMBO enables a versatile multiplexing platform to perform qualitative and quantitative analysis of proteins and miRNAs in patient serum samples simultaneously. To the best of our knowledge, this is the first method to profile protein and miRNA biomarkers to diagnose DILI in a single-step assay.

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