scholarly journals Biosynthesis of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from CO2 by a Recombinant Cupriavidus necator

2021 ◽  
Vol 8 (11) ◽  
pp. 179
Author(s):  
Kenji Tanaka ◽  
Kazumasa Yoshida ◽  
Izumi Orita ◽  
Toshiaki Fukui
Keyword(s):  
Fatty Acids ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Cupriavidus Necator ◽  
Gas Mixture ◽  
Short Chain Length ◽  
Practical Applications ◽  
Recombinant Strains ◽  
Cellular Content ◽  
High Flexibility

The copolyester of 3-hydroxybutyrate (3HB) and 3-hydoxyhexanoate (3HHx), PHBHHx, is one of the most practical kind of bacterial polyhydroxyalkanoates due to its high flexibility and marine biodegradability. PHBHHx is usually produced from vegetable oils or fatty acids through b-oxidation, whereas biosynthesis from sugars has been achieved by recombinant strains of hydrogen-oxidizing bacterium Cupriavidus necator. This study investigated the biosynthesis of PHBHHx from CO2 as the sole carbon source by engineered C. necator strains. The recombinant strains capable of synthesizing PHBHHx from fructose were cultivated in a flask using complete mineral medium and a substrate gas mixture (H2/O2/CO2 = 8:1:1). The results of GC and 1H NMR analyses indicated that the recombinants of C. necator synthesized PHBHHx from CO2 with high cellular content. When 1.0 g/L (NH4)2SO4 was used as a nitrogen source, the 3HHx composition of PHBHHx in the strain MF01∆B1/pBBP-ccrMeJ4a-emd was 47.7 ± 6.2 mol%. Further investigation demonstrated that the PHA composition can be regulated by using (R)-enoyl-CoA hydratase (PhaJ) with different substrate specificity. The composition of 3HHx in PHBHHx was controlled to about 11 mol%, suitable for practical applications, and high cellular content was kept in the strains transformed with pBPP-ccrMeJAc-emd harboring short-chain-length-specific PhaJ.

scholarly journals Draft Genome Sequence of a Chlorinated-Ethene Degrader,Cupriavidus necatorStrain PHE3-6 (NBRC 110655)

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Kenta Yonezuka ◽  
Jun Shimodaira ◽  
Michiro Tabata ◽  
Shun Nagase ◽  
Daisuke Kasai ◽  
...  
Keyword(s):  
Carbon Source ◽  
Genome Sequence ◽  
Sole Carbon Source ◽  
Draft Genome ◽  
Cupriavidus Necator ◽  
Chlorinated Ethenes ◽  
Draft Genome Sequence ◽  
Chlorinated Ethene

Cupriavidus necatorstrain PHE3-6 grows on phenol as a sole carbon source and cometabolizescis-andtrans-dichloroethenes and trichloroethene. Here, we report the draft genome sequence of PHE3-6, which provides insights into the degradation system of phenol and chlorinated ethenes.

scholarly journals A synthetic medium for the selection of yeasts able to utilize fatty acids as the sole carbon source.

1996 ◽  
Vol 42 (1) ◽  
pp. 87-91 ◽  
Author(s):  
ESTEBAN C. DELL'ANGELICA ◽  
DANIEL MILIKOWSKI ◽  
DANIEL A. SAENZ ◽  
CARLOS A. STELLA ◽  
EUGENIA H. RAMOS ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Synthetic Medium ◽  
Selection Of

The effect of volatile fatty acids as a sole carbon source on lipid accumulation by Cryptococcus albidus for biodiesel production

2011 ◽  
Vol 102 (3) ◽  
pp. 2695-2701 ◽  
Author(s):  
Qiang Fei ◽  
Ho Nam Chang ◽  
Longan Shang ◽  
Jin-dal-rae Choi ◽  
NagJong Kim ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Carbon Source ◽  
Lipid Accumulation ◽  
Sole Carbon Source ◽  
Volatile Fatty Acids ◽  
Biodiesel Production ◽  
Cryptococcus Albidus

scholarly journals Genetically Modified Strains of Ralstonia eutropha H16 with β-Ketothiolase Gene Deletions for Production of Copolyesters with Defined 3-Hydroxyvaleric Acid Contents

2012 ◽  
Vol 78 (15) ◽  
pp. 5375-5383 ◽  
Author(s):  
Nicole Lindenkamp ◽  
Elena Volodina ◽  
Alexander Steinbüchel
Keyword(s):  
Fatty Acids ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Ralstonia Eutropha ◽  
Carbon Sources ◽  
Production Capacity ◽  
Wild Type ◽  
Content Type ◽  
Storage Behavior ◽  
Copolymer Poly

ABSTRACTβ-Ketothiolases catalyze the first step of poly(3-hydroxybutyrate) [poly(3HB)] biosynthesis in bacteria by condensation of two acetyl coenzyme A (acetyl-CoA) molecules to acetoacetyl-CoA and also take part in the degradation of fatty acids. During growth on propionate or valerate,Ralstonia eutrophaH16 produces the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB-co-3HV)]. InR. eutropha, 15 β-ketothiolase homologues exist. The synthesis of 3-hydroxybutyryl-CoA (3HB-CoA) could be significantly reduced in an 8-fold mutant (Lindenkamp et al., Appl. Environ. Microbiol. 76:5373–5382, 2010). In this study, a 9-fold mutant deficient in nine β-ketothiolase gene homologues (phaA,bktB, H16_A1713, H16_B1771, H16_A1528, H16_B0381, H16_B1369, H16_A0170, andpcaF) was generated. In order to examine the polyhydroxyalkanoate production capacity when short- or long-chain and even- or odd-chain-length fatty acids were provided as carbon sources, the growth and storage behavior of several mutants from the previous study and the newly generated 9-fold mutant were analyzed. Propionate, valerate, octanoate, undecanoic acid, or oleate was chosen as the sole carbon source. On octanoate, no significant differences in growth or storage behavior were observed between wild-typeR. eutrophaand the mutants. In contrast, during the growth on oleate of a multiple mutant lackingphaA,bktB, and H16_A0170, diminished poly(3HB) accumulation occurred. Surprisingly, the amount of accumulated poly(3HB) in the multiple mutants grown on gluconate differed; it was much lower than that on oleate. The β-ketothiolase activity toward acetoacetyl-CoA in H16ΔphaAand all the multiple mutants remained 10-fold lower than the activity of the wild type, regardless of which carbon source, oleate or gluconate, was employed. During growth on valerate as a sole carbon source, the 9-fold mutant accumulated almost a poly(3-hydroxyvalerate) [poly(3HV)] homopolyester with 99 mol% 3HV constituents.

GROWTH OF FUSARIUM DIVERSISPORUM SHERB. ON LONG-CHAINn-FATTY ALCOHOLS OR CHOLESTEROL AS THE SOLE CARBON SOURCE

1967 ◽  
Vol 13 (2) ◽  
pp. 121-136 ◽  
Author(s):  
Rudolf G. Strobel ◽  
Herbert Quinn ◽  
Willy Lange
Keyword(s):  
Fatty Acids ◽  
Cell Wall ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Unsaturated Fatty Acids ◽  
Submerged Cultures ◽  
Reserve Material ◽  
Lipid Contents ◽  
Cell Components ◽  
Metabolic Action

In well-aerated submerged cultures of Fusarium diversisporum Sherb. with n-hexadecanol or n-heptadecanol (which are virtually water insoluble) as the sole carbon source, the alkanols do not undergo extracellular chemical changes before assimilation but move, unchanged, through the cell wall faster than they can be metabolized by the organism and thus may constitute up to one-half of the total lipids in the cells. The alkanols are initially oxidized at the hydroxylated terminal carbon atom to fatty acids without loss of carbon. The fatty acids are subject to further metabolic action. Apparently carbon, not needed immediately for energy and for synthesis of cell components, is transformed into triglycerides as a reserve material. In these triglycerides, the distribution of the saturated and the unsaturated fatty acids between the primary and the secondary positions of the glycerol moiety is one typical of a vegetable lipid. Interesting differences exist, particularly in the sterol ester and phospho- or glyco-lipid contents, between mycelia grown on sucrose, hexadecanol, or heptadecanol. These cell constituents may possibly be involved in alkanol transport across the cell wall. The mold also assimilates cholesterol but has difficulty in metabolizing it.

scholarly journals Role of Sinorhizobium meliloti and Escherichia coli Long-Chain Acyl-CoA Synthetase FadD in Long-Term Survival

2020 ◽  
Vol 8 (4) ◽  
pp. 470
Author(s):  
Ángel de la Cruz Pech-Canul ◽  
Geovanny Rivera-Hernández ◽  
Joaquina Nogales ◽  
Otto Geiger ◽  
María J. Soto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fatty Acids ◽  
Carbon Source ◽  
Stationary Phase ◽  
Sole Carbon Source ◽  
Sinorhizobium Meliloti ◽  
Membrane Lipids ◽  
Term Survival ◽  
Long Chain

FadD is an acyl-coenzyme A (CoA) synthetase specific for long-chain fatty acids (LCFA). Strains mutated in fadD cannot produce acyl-CoA and thus cannot grow on exogenous LCFA as the sole carbon source. Mutants in the fadD (smc02162) of Sinorhizobium meliloti are unable to grow on oleate as the sole carbon source and present an increased surface motility and accumulation of free fatty acids at the entry of the stationary phase of growth. In this study, we found that constitutive expression of the closest FadD homologues of S. meliloti, encoded by sma0150 and smb20650, could not revert any of the mutant phenotypes. In contrast, the expression of Escherichia coli fadD could restore the same functions as S. meliloti fadD. Previously, we demonstrated that FadD is required for the degradation of endogenous fatty acids released from membrane lipids. Here, we show that absence of a functional fadD provokes a significant loss of viability in cultures of E. coli and of S. meliloti in the stationary phase, demonstrating a crucial role of fatty acid degradation in survival capacity.

A survey of filamentous foaming in activated sludge plants in Hong Kong

1994 ◽  
Vol 30 (11) ◽  
pp. 251-254 ◽  
Author(s):  
H. Chua ◽  
K. Y. Le
Keyword(s):  
Fatty Acids ◽  
Hong Kong ◽  
Carbon Source ◽  
Activated Sludge ◽  
Sole Carbon Source ◽  
Sewage Treatment ◽  
Municipal Sewage ◽  
Filamentous Foaming ◽  
Nocardia Amarae

Of five major secondary sewage treatment works surveyed during 1993 in Hong Kong, two had frequent foaming in the activated sludge plants which resulted in unmanageable operational problems. In this study, branched-filamentous Nocardia amarae was identified as the main causative bacteria in foaming sludges. In vitro studies showed that fatty acids that are commonly found in municipal sewage could be utilized by N. amarae as the sole carbon source, and presence of these fatty acids in sewage could stimulate growth.

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