scholarly journals Measurable Cytokine Concentrations in Pig Seminal Plasma Are Modified by Semen Handling and Storage

Biology ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 276
Author(s):  
Lorena Padilla ◽  
Isabel Barranco ◽  
Inmaculada Parrilla ◽  
Xiomara Lucas ◽  
Heriberto Rodriguez-Martinez ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Biological Fluids ◽  
Storage Conditions ◽  
Reliable Measurement ◽  
Short Term ◽  
Gm Csf ◽  
Long Term Storage ◽  
And Storage ◽  
Term Storage

Sample handling and storing are critical steps for the reliable measurement of circulating biomolecules in biological fluids. This study evaluates how cytokine measurements in pig seminal plasma (SP) vary depending on semen handling and SP storage. Thirteen cytokines (GM-CSF, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18 and TNFα) were measured using Luminex xMAP® technology in individual seminal plasma (SP) samples (n = 62) from healthy breeding boars. Three separate experiments explored the delay (2 h and 24 h) in SP collection after ejaculation (Experiment 1) and SP storage, either short-term (5 °C, −20 °C and −80 °C for 72 h, Experiment 2) or long-term (at −20 °C and −80 °C for two months, Experiment 3), before analysis. Levels in fresh SP-samples were used as baseline control values. Delays in SP harvesting of up to 24 h did not substantially impact SP cytokine measurements. Some cytokines showed instability in stored SP samples, mainly in long-term storage. Ideally, cytokines in pig SP should be measured in fresh samples harvested within 24 h after ejaculation. If storage of SP is imperative, storage conditions should be adjusted for each cytokine.

scholarly journals Management of Apple Maturity and Postharvest Storage Conditions to Increase Polyphenols in Cider

HortScience ◽  
2019 ◽  
Vol 54 (1) ◽  
pp. 143-148 ◽  
Author(s):  
Brianna L. Ewing ◽  
Gregory M. Peck ◽  
Sihui Ma ◽  
Andrew P. Neilson ◽  
Amanda C. Stewart
Keyword(s):  
The United States ◽  
Storage Conditions ◽  
Short Term ◽  
Postharvest Storage ◽  
Total Polyphenols ◽  
Fruit Storage ◽  
Long Term Storage ◽  
Short Term Storage ◽  
Term Storage

Hard cider production in the United States has increased dramatically during the past decade, but there is little information on how harvest and postharvest practices affect the chemistry of the resulting cider, including concentrations of organoleptically important flavanols. For 2 years we assessed fruit, juice, and cider from a total of five apple (Malus ×domestica Borkh.) cultivars in two experiments: sequential harvests and postharvest storage. Different cultivars were used in 2015 and 2016 with the exception of ‘Dabinett’, which was assessed in both years. There were no differences in polyphenol concentrations in cider made from fruit that was harvested on three separate occasions over a 4-week period in either 2015 or 2016. Fruit storage durations and temperatures had little influence on the chemistry when the experiment was conducted in 2015, but polyphenol concentration was greater after storage in the 2016 experiment. In 2016, total polyphenols in ‘Dabinett’ ciders were 51% greater after short-term storage at 10 °C and 67% greater after long-term storage at 1 °C than the control, which was not subjected to a storage treatment. In 2016, total polyphenols in ‘Binet Rouge’ ciders were 67% greater after short-term storage at 10 °C and 94% greater after long-term storage at 1 °C than the control. Although results varied among cultivars and harvest years, storing apples for longer periods of time and at warmer temperatures may be a strategy to increase polyphenol, particularly flavanol, concentrations in hard cider.

scholarly journals ANTIOXIDANT PROPERTIES AND FRUIT QUALITY DURING LONG-TERM STORAGE OF “ROCHA” PEAR: EFFECTS OF MATURITY AND STORAGE CONDITIONS

2010 ◽  
Vol 33 (1) ◽  
pp. 1-20 ◽  
Author(s):  
FILIPE J.P. SILVA ◽  
MARIA HELENA GOMES ◽  
FERNANDA FIDALGO ◽  
JOSÉ A. RODRIGUES ◽  
DOMINGOS P.F. ALMEIDA
Keyword(s):  
Fruit Quality ◽  
Antioxidant Properties ◽  
Storage Conditions ◽  
Long Term Storage ◽  
And Storage ◽  
Term Storage

scholarly journals A feasible method to extract DNA from the cambium of high-canopy trees: from harvest to assessment

2020 ◽  
Vol 50 (4) ◽  
pp. 335-338
Author(s):  
Érica MANGARAVITE ◽  
Vanessa TERRA ◽  
Eric Koiti Okiyama HATTORI ◽  
Thaís Carolina da Silva DAL’SASSO ◽  
Leonardo Lopes BHERING ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Storage Conditions ◽  
Microsatellite Genotyping ◽  
Short Term ◽  
Feasible Method ◽  
Long Term Storage ◽  
Canopy Trees ◽  
Amazonian Forests ◽  
Term Storage

ABSTRACT Many tropical trees have high canopies and their leaves are not accessible. Thus, the use of tissue from a more accessible organ (cambium) for DNA extraction may be an alternative for molecular studies. We adapted a feasible methodology for extracting genomic DNA from cambium tissue harvested in the field for the assessment with PCR. We tested three storage conditions (two buffers and a silica gel) and four periods of time after harvest. We used previously described protocols and tested them on three species that occur in Amazonian forests and other biomes: Anadenanthera peregrina var. peregrina, Cedrela fissilis, and Ceiba speciosa. Our protocol obtained suitable PCR-grade genomic DNA for DNA sequencing and microsatellite genotyping. We recommend the use of silica for long-term storage and the buffer with ascorbic acid for short-term storage.

Response of Longleaf Pine Seeds to Storage Conditions and Pregermination Treatments

1993 ◽  
Vol 17 (4) ◽  
pp. 174-179 ◽  
Author(s):  
James P. Barnett ◽  
John P. Jones
Keyword(s):  
Seed Quality ◽  
Longleaf Pine ◽  
Pinus Palustris ◽  
Storage Conditions ◽  
Operational Conditions ◽  
Long Term Storage ◽  
Processing And Storage ◽  
And Storage ◽  
Term Storage

Abstract Although longleaf pine (Pinus palustris Mill.) seeds are considered the most susceptible of the southern pines to damage during collection, processing, and storage, results of these studies show that high seed quality can be assured for periods up to 20 yr through proper handling and storing techniques. Recommendations for long-term storage include drying seeds to moisture contents of 10% or less and storing at subfreezing temperatures, preferably near 0°F. Reevaluation of stratification treatments applied under operational conditions indicates that the soaking in water that is necessary for seed imbibition reduces total germination in an amount proportional to the length of the soak. Stratification is not recommended except under very controlled conditions. South. J. Appl. For. 17(4):174-179.

scholarly journals Environment is crucial to the cooking time of beans

2012 ◽  
Vol 32 (3) ◽  
pp. 573-578 ◽  
Author(s):  
Bruna Arruda ◽  
Altamir Frederico Guidolin ◽  
Jefferson Luís Meirelles Coimbra ◽  
Jaqueline Battilana
Keyword(s):  
Storage Time ◽  
Storage Condition ◽  
Storage Conditions ◽  
Cooking Time ◽  
Dependent Manner ◽  
Simple Linear Regression ◽  
Long Term Storage ◽  
And Storage ◽  
Term Storage

The objectives of this study were to understand how genotype, storage time, and storage conditions affect cooking time of beans and to indicate storage techniques that do not affect the cooking time. The grains were subjected to five different storage periods and six different storage conditions. The cooking time was estimated using the Mattson Cooker. The data were subjected to analysis of variance and a subsequent adjustment of simple linear regression for deployment of the interactions between the factors. Contrasts were used to determine the best levels of the factor storage condition. Genotype did not impact cooking time when the storage time and storage conditions were considered. Time and storage conditions affect the cooking time of beans in a dependent manner, but time of storage had the biggest influence. The best conditions for long-term storage of beans ensuring a smaller increase in cooking time is plastic storage at low temperatures. Thus, plastic freezer storage is a practical alternative for consumers.

scholarly journals Dry storage of sperm: applications in primates and domestic animals

2006 ◽  
Vol 18 (2) ◽  
pp. 1 ◽  
Author(s):  
Stuart A. Meyers
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
Short Term ◽  
Long Term Storage ◽  
Space Requirements ◽  
Thermotropic Phase Transitions ◽  
And Storage ◽  
Cryogenic Conditions ◽  
Term Storage

Cryopreservation of spermatozoa, oocytes and embryos, as well as somatic cells or cell lines for cloning from cells, are all options for the long-term storage of unique genotypes and endangered species. Spermatozoal cryopreservation and storage currently require liquid nitrogen or ultralow refrigeration-based methods for long- or short-term storage, which requires routine maintenance and extensive space requirements. The preservation of stem cells also has strict requirements for long-term storage to maintain genetic integrity. Dessicated (lyopreserved) sperm and stem cells will provide an unprecedented type of long-term storage without the need for expensive and burdensome cryogenic conditions. Experiments were conducted to determine an effective intracellular concentration of the lyoprotectant trehalose. High-pressure liquid chromatography studies revealed that trehalose can be incorporated into mature sperm cells as well as spermatogonial stem cells from rhesus monkeys. In addition, using fourier transform infrared spectroscopy, we determined that thermotropic phase transitions for fresh ejaculates from rhesus monkey and stallion sperm occurred at 10–15, 33–37 and 55–59°C. Preliminary studies in our laboratory have indicated that spermatogonial stem cells can be dried to <3 g g−1 water and maintain viability following rehydration. Studies in our laboratory have provided preliminary results suggesting that the desiccated storage of sperm and spermatogonial stem cells may be a viable alternative to conventional cryopreservation.

Human plasma stability during handling and storage: impact on NMR metabolomics

The Analyst ◽  
2014 ◽  
Vol 139 (5) ◽  
pp. 1168-1177 ◽  
Author(s):  
Joana Pinto ◽  
M. Rosário M. Domingues ◽  
Eulália Galhano ◽  
Cristina Pita ◽  
Maria do Céu Almeida ◽  
...  
Keyword(s):  
Human Plasma ◽  
Room Temperature ◽  
Storage Conditions ◽  
Plasma Composition ◽  
Long Term Storage ◽  
Short And Long Term ◽  
The Stability ◽  
And Storage ◽  
Term Storage

The stability of human plasma composition was investigated by NMR, considering different collection tubes, time at room temperature (RT), short- and long-term storage conditions and up to 5 consecutive freeze–thaw cycles.

MODERN PROBLEMATICS AND ANALYSIS OF EFFICIENCY OF APPLICATION OF DIFFERENT APPROACHES TO DESTRUCTION OF INFORMATION ON MAGNETIC MEDIA

2019 ◽  
Vol 2 (12) ◽  
pp. 154-164
Author(s):  
O. Semenenko ◽  
O. Vodchyts ◽  
V. Koverga ◽  
R. Lukash ◽  
O. Lutsenko
Keyword(s):  
Armed Forces ◽  
Information Storage ◽  
Storage Device ◽  
Magnetic Media ◽  
Long Term Storage ◽  
Physical Impact ◽  
And Storage ◽  
Term Storage

The introduction and active use of information transmission and storage systems in the Ministry of Defense (MoD) of Ukraine form the need to develop ways of guaranteed removal of data from media after their use or long-term storage. Such a task is an essential component of the functioning of any information security system. The article analyzes the problems of guaranteed destruction of information on magnetic media. An overview of approaches to the guaranteed destruction of information on magnetic media of different types is presented, and partial estimates of the effectiveness of their application are given by some generally accepted indicators of performance evaluation. The article also describes the classification of methods of destruction of information depending on the influence on its medium. The results of the analysis revealed the main problems of application of software methods and methods of demagnetization of the information carrier. The issue of guaranteed destruction of information from modern SSD devices, which are actively used in the formation of new systems of information accumulation and processing, became particularly relevant in the article. In today's conditions of development of the Armed Forces of Ukraine, methods of mechanical and thermal destruction are more commonly used today. In the medium term, the vector of the use of information elimination methods will change towards the methods of physical impact by the pulsed magnetic field and the software methods that allow to store the information storage device, but this today requires specialists to develop new ways of protecting information in order to avoid its leakage.

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