scholarly journals Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering

Cells ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 1046 ◽  
Author(s):  
Božič ◽  
Sitar ◽  
Junkar ◽  
Štukelj ◽  
Pajnič ◽  
...  
Keyword(s):  
Light Scattering ◽  
Blood Plasma ◽  
Extracellular Vesicles ◽  
Viscosity Coefficient ◽  
Plausible Model ◽  
Average Shape ◽  
Key Factor ◽  
Spherical Vesicles ◽  
In Blood Plasma

Extracellular vesicles (EVs) isolated from biological samples are a promising material for use in medicine and technology. However, the assessment methods that would yield repeatable concentrations, sizes and compositions of the harvested material are missing. A plausible model for the description of EV isolates has not been developed. Furthermore, the identity and genesis of EVs are still obscure and the relevant parameters have not yet been identified. The purpose of this work is to better understand the mechanisms taking place during harvesting of EVs, in particular the role of viscosity of EV suspension. The EVs were harvested from blood plasma by repeated centrifugation and washing of samples. Their size and shape were assessed by using a combination of static and dynamic light scattering. The average shape parameter of the assessed particles was found to be ρ ~ 1 (0.94–1.1 in exosome standards and 0.7–1.2 in blood plasma and EV isolates), pertaining to spherical shells (spherical vesicles). This study has estimated the value of the viscosity coefficient of the medium in blood plasma to be 1.2 mPa/s. It can be concluded that light scattering could be a plausible method for the assessment of EVs upon considering that EVs are a dynamic material with a transient identity.

scholarly journals Role of fibronectin under conditions of doxorubicin action

2015 ◽  
Vol 6 (1) ◽  
pp. 17-22
Author(s):  
A. I. Shevtsova ◽  
G. A. Ushakova
Keyword(s):  
Blood Plasma ◽  
Total Protein ◽  
Protein Concentration ◽  
Male Rats ◽  
Antitumor Action ◽  
Total Protein Concentration ◽  
Monospecific Antibodies ◽  
Anthracycline Chemotherapy ◽  
In Blood Plasma

There is no standard as to treatment of anthracycline chemotherapy complications. The reduction of cytotoxic drugs toxicity without weakening of their antitumor action remains relevant. The extracellular matrix which key component is fibronectin is present in all tissues and it continuously undergoes controlled remodeling. So, the purpose of our work was to study the level of fibronectin in the experimental model of doxorubicin-induced cardiomyopathy and effects of this cytostatic and its co-administration with antioxidants of different nature.The level of fibronectin was measured by ELISA using monospecific antibodies against fibronectin (Sigma, USA), secondary anti-IgG labeled with horseradish peroxidase (Sigma, USA) and fibronectin standard (Sigma, USA). The study was conducted on Wistar male rats with weight of 210 ± 50 g which were divided into 4 groups by 8 animals in each group: 1 – control, rats receiving saline i/p; 2 – doxorubicin 1 mg/kg i/p once a week during 4 weeks; 3 – doxorubicin by the same scheme plus 1% 2-oxoglutarate in drinking water during 4 weeks;4 – doxorubicin by the same scheme and korvitin injection 30 min before doxorubicin application once a week during 4 weeks. Obtained data indicate the effect of doxorubicin to decrease in index mass heart in 38% of animals compared to control animals; decrease in total protein concentration by 8% (Р < 0,05) and increase of the level of fibronectin by 67% (P < 0,001) in blood plasma of rats and decrease in the level of fibronectin in the heart extract by 19% (Р < 0,05) under development of doxorubicin-induced cardiotoxicity. Increased fibronectin concentration in blood plasma had strong correlation with decreased total protein concentration in blood (r=0,80) and heart extract (r=0,59) in rats with doxorubicin-induced cardiomiophaty indicating the sensitive reaction of fibronectin to development of metabolic disorders under doxorubicin influence. 

scholarly journals Role of water soluble vitamins in the reduction diet of an amateur sportsman

2018 ◽  
Vol 13 (1) ◽  
pp. 163-173 ◽  
Author(s):  
Małgorza Szczuko ◽  
Rafał Migrała ◽  
Arleta Drozd ◽  
Marcin Banaszczak ◽  
Dominika Maciejewska ◽  
...  
Keyword(s):  
Vitamin C ◽  
Blood Plasma ◽  
Adult Male ◽  
Water Soluble ◽  
Diet Supplements ◽  
Before And After ◽  
In Blood Plasma ◽  
Diet Supplementation

AbstractThis study is aimed at determining the content of water soluble vitamins in amateur sportsmen before and after the use of reduction diet. Twenty adult male amateur sportsmen aged between 20 and 43 qualified for this study. The participants adhered to individually adjusted reduction diets for 3 months. Diet supplements were excluded from the diet during that time. Vitamins were analyzed before and after the reduction diet using HPLC. The following vitamins were analyzed: B1, B2, B3, PP, B5, B6, B7, B9, B12 and vitamin C. Statistically significant increase of riboflavin in blood plasma (p=0.0254) and statistically significant (p=0.048) decrease in the concentration of niacin was found when compared to the period before the diet. Appropriate level of riboflavin can be obtained by the consumption of proper amount of diary in the diet. Supplementation with PP is advised and, in case of improper diet, the supplementation of B1, B7 and B12 may also be considered.

scholarly journals Nanoscale flow cytometry for immunophenotyping and quantitating extracellular vesicles in blood plasma

Nanoscale ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 2012-2025 ◽  
Author(s):  
Nikki Salmond ◽  
Karan Khanna ◽  
Gethin R. Owen ◽  
Karla C. Williams
Keyword(s):  
Flow Cytometry ◽  
Blood Plasma ◽  
Extracellular Vesicles ◽  
Small Particles ◽  
In Blood Plasma

Nanoscale flow cytometry permits analysis of small particles in unpurified plasma and supports identification and quantitation of different particle populations.

scholarly journals The effect of intestinal coccidiosis (Eimeria acervulina) on blood and tissue ascorbic acid concentrations

1979 ◽  
Vol 42 (1) ◽  
pp. 97-103 ◽  
Author(s):  
Ishak T. Kechik ◽  
A. H. Sykes
Keyword(s):  
Ascorbic Acid ◽  
Blood Plasma ◽  
Experimental Infection ◽  
Adrenal Glands ◽  
Prepatent Period ◽  
Adrenal Weight ◽  
Eimeria Acervulina ◽  
In Blood Plasma

1. Two experiments were conducted to investigate the effect of intestinal coccidiosis (Eimeria acervulina) on blood plasma and tissue ascorbic acid (AA) concentrations in chicks, and a third experiment was carried out to examine the role of dietary AA in this infection.2. Experimental infection with intestinal coccidiosis (Eimeria acervulina) brought about a depletion in the concentration of AA in blood plasma, duodenum, jejunum, ileum, liver and the adrenal glands. Generally, these changes were obtained after the prepatent period of 4–5 d of the infection.3. Dietary AA at 1000 mg/kg prevented the depletion in plasma and tissue AA levels but did not significantly affect adrenal weight.

Determination of Semicarbazide-Sensitive Amine Oxidase Activity in Blood Plasma by a Light Scattering Technique

2012 ◽  
Vol 45 (18) ◽  
pp. 2774-2784 ◽  
Author(s):  
Xuan Tan ◽  
Wei-Qing Rang ◽  
Yong-Sheng Wang ◽  
Hui-Xian Yang ◽  
Jin-Hua Xue ◽  
...  
Keyword(s):  
Light Scattering ◽  
Blood Plasma ◽  
Oxidase Activity ◽  
Amine Oxidase ◽  
Scattering Technique ◽  
Light Scattering Technique ◽  
Amine Oxidase Activity ◽  
In Blood Plasma

scholarly journals Actuality of study the vascular endothelial growth factor in patients with arteriovenous malformation

2020 ◽  
Vol 30 (4) ◽  
pp. 59-67
Author(s):  
I.V. Altman
Keyword(s):  
Growth Factor ◽  
Endovascular Treatment ◽  
Blood Plasma ◽  
Arteriovenous Malformations ◽  
Medical Literature ◽  
Perioperative Period ◽  
Vascular Growth ◽  
Vascular Growth Factor ◽  
In Blood Plasma

Objective – to determine the role of endothelial vascular growth factor (VEGF) in the formation, progression of arteriovenous malformations (AVM), the occurrence of relapses of this disease and to justify the need to study the level of VEGF in blood plasma in patients with AVM.Materials and methods. The analysis of 15 sources of scientific and medical literature on the etio-logy and pathogenesis of AVM. Recent data of studies of the role of VEGF in the formation, progression of AVM, occurrence of relapses of this disease have been systematized. The own results of the study of the level of VEGF in blood plasma in 19 patients with AVM of different localization are presented.Results. The important role of increasing the level of VEGF in the formation, progression of AVM, both according to scientific and medical literature and according to own research, has been proved. The necessity to study the level of VEGF in blood plasma in patients with AVM in the perioperative period to improve the effectiveness of complex treatment of such patients is substantiated.Conclusions. Endothelial vascular growth factor plays an important role in the formation, progression of arteriovenous malformations. Increased level of VEGF in blood plasma in patients with AVM was noted. Increased levels of VEGF in blood plasma after surgical or endovascular treatment may cause relapses of the disease. Changes in the level of VEGF in blood plasma after surgical or endovascular treatment have not been studied at present. Studying the level of VEGF in blood plasma before and after surgical and endovascular treatment opens new possibilities for the use of drugs that block the action of VEGF as a neoadjuvant therapy for the prevention of recurrence or regression of stroke.

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