scholarly journals Chloroplast DNA Diversity in Populations of P. sylvestris L. from Middle Siberia and the Romanian Carpathians

Forests ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1757
Author(s):  
Marina Sheller ◽  
Elena Ciocîrlan ◽  
Pavel Mikhaylov ◽  
Sergey Kulakov ◽  
Nadezhda Kulakova ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Scots Pine ◽  
Sustainable Management ◽  
Forest Ecosystems ◽  
Chloroplast Microsatellite ◽  
High Genetic Diversity ◽  
Geographic Population ◽  
Romanian Carpathians ◽  
Management And Conservation ◽  
Middle Siberia

Scots pine is one of the dominant conifer species in forest ecosystems of the boreal zone in Eurasia. Knowledge of the genetic structure and the level of genetic variability of Scots pine populations is relevant for the development of measures aimed at conservation of species’ diversity. In this study, we used ten paternally inherited chloroplast microsatellite loci to investigate the genetic diversity of nineteen Scots pine populations from Middle Siberia and the Romanian Carpathians. The results of the study showed high genetic diversity (HCP = 0.91–1.00) in all of the investigated populations. The cpSSR analysis yielded a total of 158 haplotypes. The majority of the haplotypes (85%) were detected only once (unique haplotypes). Three common haplotypes were found between the Carpathian and the Siberian populations of Scots pine. Analysis of molecular variance (AMOVA) showed that only 3% of the variation occurred among populations from Middle Siberia and 6% of the variation existed among populations from the Carpathian Mountains. Overall, we found a weak geographic population structure in Scots pine from Middle Siberia and the Romanian Carpathians. The present study on genetic diversity in the Siberian and the Carpathian populations of Scots pine may contribute to the sustainable management and conservation of Scots pine genetic resources in Middle Siberia and the Romanian Carpathians.

Genetic Diversity and Population Structure of Phyllosticta citriasiana in China

2020 ◽  
Author(s):  
Yibing Zeng ◽  
Tao Xiong ◽  
Bei Liu ◽  
Elma Carstens ◽  
Xiangling Chen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Mating Type ◽  
Tan Spot ◽  
Long Distance ◽  
High Genetic Diversity ◽  
Geographic Population ◽  
Sexual And Asexual Reproduction ◽  
Geographic Populations ◽  
Genetic Clusters

Phyllosticta citriasiana is the causal agent of citrus tan spot, an important pomelo disease in Asia. At present, there is little or no information on the epidemiology or population structure of P. citriasiana. Using simple sequence repeat (SSR) markers, 94 isolates obtained from three pomelo production regions in southern/southeastern China were analyzed. The analyses showed high genetic diversity in each of the three geographic populations. A STRUCTURE analysis revealed two genetic clusters among the 94 isolates, one geographic population was dominated by genotypes in one cluster while the other two geographic populations were dominated by genotypes of the second cluster. P. citriasiana has a heterothallic mating system with two idiomorphs, MAT1-1 and MAT1-2. Analyses using mating type-specific primers revealed that both mating types were present in all three geographic populations, and in all three populations the mating type ratios were in equilibrium. Although the sexual stage of the fungus has not been discovered yet, analyses of allelic associations indicated evidence for sexual and asexual reproduction within and among populations. Despite the observed genetic differentiation among the three geographic populations, evidence for long-distance gene flow was found.

scholarly journals Genetic Diversity and Differentiation of Alpinia Oxyphylla Miquel as Revealed by AFLP Markers

2021 ◽  
Author(s):  
Kun Pan ◽  
Jie Hou ◽  
Wenqin Su ◽  
Bo Yi ◽  
Bingmiao Gao
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Gene Diversity ◽  
Germplasm Conservation ◽  
Aflp Markers ◽  
Group Method ◽  
High Genetic Diversity ◽  
Geographic Population ◽  
Arithmetic Average ◽  
Population Structure Analysis

Abstract In this study, we analyzed the genetic diversity and population structure of 90 A. oxyphylla accessions from Hainan island using amplified fragment length polymorphism (AFLP) markers. These 90 accessions were composed of 15 populations from different geographic locations and divided into 4 clusters (A, B, C, and D) using the Unweighted pair group method based on arithmetic average (UPGMA). The genetic similarity between individuals ranged from 0.47 to 1.00 (average of 0.74), and most accessions from the same geographic population were grouped together. Principal coordinate analysis (PCA) showed a clear distinction among three clusters (A, B and C). Based on the loci information, the population structure analysis results by STRUCTURE and TESS were consistent with the clustering of PCA. Nine AFLP primer combinations generated 1537 polymorphic bands displaying rich polymorphism, thus indicating high genetic diversity among these 15 populations with an average Nei’s gene diversity of 0.1328 ± 0.160. In conclusion, AFLP markers efficiently analyzed the genetic diversity in A. oxyphylla, demonstrating highly significant genetic variation within and among populations. However, intrapopulation genetic variance was much higher than interpopulation variability, suggesting that efforts should be made for in situ germplasm conservation and resistant varieties cultivation.

scholarly journals Estimating the genetic diversity and structure of Quercus trojana Webb populations in Italy by SSRs: implications for management and conservation

2017 ◽  
Vol 47 (3) ◽  
pp. 331-339 ◽  
Author(s):  
Maddalena Carabeo ◽  
Marco Cosimo Simeone ◽  
Marcello Cherubini ◽  
Chiara Mattia ◽  
Francesca Chiocchini ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Eastern Mediterranean ◽  
Conservation Strategies ◽  
Gene Pools ◽  
High Genetic Diversity ◽  
Genetic Diversity And Structure ◽  
Main Gene ◽  
Nuclear Microsatellite ◽  
Fragmented Distribution ◽  
Management And Conservation

Studying the genetic diversity and structure of the current forest populations is essential for evaluating the ability to survive future biotic and abiotic changes and planning conservation strategies. Quercus trojana Webb is an eastern Mediterranean tree species with a fragmented distribution range, and its westernmost outposts are located in southern Italy. The demand for timber and cropland over the centuries has severely reduced its occurrence in this part of the range. We assessed the genetic diversity and structure of the extant Italian populations of Q. trojana and derived conservation guidelines. A total of 322 samples were genotyped with six polymorphic nuclear microsatellite markers. A high genetic diversity in all populations, two main gene pools, and a highly divergent single population were observed. Based on the allelic richness and heterozygosity estimation, we identified populations that can be considered as valuable source material for conservation programs and those requiring adequate measures to reestablish gene flow and reduce fragmentation. Finally, a comparison with a set of eastern Mediterranean samples indicated a relationship between the Italian and the Greek gene pools. The need to protect these marginal, disjunct populations was further reinforced.

scholarly journals Genetic Diversity and Population Genetic Structure of Ancient Platycladus orientalis L. (Cupressaceae) in the Middle Reaches of the Yellow River by Chloroplast Microsatellite Markers

Forests ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 592
Author(s):  
Bei Cui ◽  
Ping Deng ◽  
Sheng Zhang ◽  
Zhong Zhao
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Differentiation ◽  
Yellow River ◽  
The Yellow River ◽  
Group Method ◽  
Chloroplast Microsatellite ◽  
Genetic Population ◽  
Geographic Population ◽  
Platycladus Orientalis

Ancient trees are famous for their life spans of hundreds or even thousands of years. These trees are rare, a testament to history and are important for scientific research. Platycladus orientalis, with the longest life span and a beautiful trunk, has become the most widely planted tree species and is believed to be sacred in China. Extensive declines in habitat area and quality pose the greatest threats to the loss of genetic diversity of ancient P. orientalis trees in the middle reaches of the Yellow River. Strengthening the protection of P. orientalis genetic resources is of great significance for the long-term development of reasonable conservation and breeding strategies. To better understand the genetic diversity and population structure of P. orientalis, we successfully analyzed four polymorphic chloroplast simple sequence repeat (cpSSR) loci and applied them to diversity and population structure analyses of 202 individuals from 13 populations in the middle reaches of the Yellow River. Based on the cpSSR data, 16 alleles were detected across 202 individuals, and a moderate level of genetic diversity was inferred from the genetic diversity parameters (H = 0.367 and AR = 1.964). The mean pairwise genetic differentiation coefficient (Fst) between populations was 0.153, indicating relatively high genetic population differentiations. Analysis of molecular variance (AMOVA) showed that only 8% of the variation occurred among populations. Structure analysis divided the 13 P. orientalis populations into two groups with no significant geographic population structure, which was consistent with the unweighted pair group method with arithmetic mean (UPGMA) and Mantel test results. These results may indicate that transplanting and cultivation by ancient human activities are the main factors responsible for the revealed pattern of genetic differentiation of ancient P. orientalis populations. Our research is of great significance for the future establishment of protection schemes and scientific breeding of P. orientalis.

scholarly journals Genetic diversity and relationships of indigenous and newly bred Bulgarian grape cultivars assessed by nuclear and chloroplast markers

OENO One ◽  
2012 ◽  
Vol 46 (2) ◽  
pp. 113 ◽  
Author(s):  
Teodora Dzhambazova ◽  
Ivan Tsvetkov ◽  
Ilian Simeonov ◽  
Miroslav Ivanov ◽  
Zdravko Nakov ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationships ◽  
Microsatellite Analysis ◽  
Genetic Identification ◽  
Chloroplast Microsatellite ◽  
High Genetic Diversity ◽  
Local Varieties ◽  
Chloroplast Markers ◽  
Grape Cultivars

<p style="text-align: justify;"><strong>Aim</strong>: Assessment of the genetic diversity and relationships in a group of 31 Bulgarian grape accessions through microsatellite markers.</p><p style="text-align: justify;"><strong>Methods and results</strong>: Thirty-one accessions, including 20 old rare local and 11 newly bred varieties were characterized through 13 nuclear and 5 chloroplast microsatellite loci. The genetic diversity (0.81±0.01) obtained for the investigated group of cultivars was comparable to those reported for other grapevine germplasms. The low PI value (1.0x10<sup>-16</sup>) allowed proper genetic identification and determination of synonyms. Microsatellite analysis of the 31 accessions resulted in 26 unique genotypes and 2 groups of synonyms. Four cases of supposed synonymy with local Bulgarian and foreign cultivars were rejected. Three chlorotypes, B, C and D, were defined among the studied cultivars, with a prevalence of chlorotype C (62%).</p><p style="text-align: justify;"><strong>Conclusion</strong>: The high genetic diversity found in the set of old rare grapevines demonstrated their importance as a rich source of alleles for breeding. The pattern of chlorotype distribution observed among local varieties confirmed the previous results and supports the hypothesis of an Eastern origin of local Bulgarian cultivars.</p><p style="text-align: justify;"><strong>Significance and impact of the study</strong>: The obtained results provide an important support for the preservation of grape biodiversity in Bulgaria as well as for the clarification of genetic relationships between local and foreign cultivars.</p>

scholarly journals The Genetic Structure of Sorocea bonplandii in Southern Brazilian Forest Fragments: AFLP Diversity

2007 ◽  
Vol 56 (1-6) ◽  
pp. 51-58
Author(s):  
A. R. Ruschel ◽  
R. O. Nodari ◽  
B. M. Moerschbacher
Keyword(s):  
Genetic Diversity ◽  
Atlantic Forest ◽  
Sustainable Management ◽  
Genetic Erosion ◽  
Management Plan ◽  
Genetic Distances ◽  
Conservation Strategies ◽  
Forest Fragments ◽  
High Genetic Diversity ◽  
Key Species

Abstract To analyse possible genetic erosion due to fragmentation in the Brazilian Atlantic Forest ecosystem, we investigated the genetic diversity within and among one large and six small populations of one of the key species of this ecosystem, Sorocea bonplandii, using AFLP analyses of 468 plants. Eight primer pairs yielded 299 polymorphic fragments for analysis. S. bonplandii was characterized by an unusually high genetic diversity within the species and also within individual populations, around 94% of the total genetic diversity occurred within populations. Genetic distances between populations were low in spite of extensive fragmentation. Genetic distance was significantly correlated with geographical distances between fragments, but these differences may have existed before fragmentation. Our results have direct implications for sustainable management of S. bonplandii, indicating that conservation strategies might be based on a random sample of trees taken throughout the Atlantic forest. However, the minimum population size required for maintaining the huge genetic diversity of this species is unknown. In order to establish a sustainable management plan for the species, further ecological studies are needed.

scholarly journals Variability of Scots pine (Pinus sylvestris L.) called Tabórz pine (Forest District Miłomłyn) expressed in analysis of morphology of needle traits and polymorphism of microsatellite DNA

2017 ◽  
Vol 78 (2) ◽  
pp. 136-148 ◽  
Author(s):  
Paulina Lesiczka ◽  
Ewa M. Pawlaczyk ◽  
Bartosz Łabiszak ◽  
Lech Urbaniak
Keyword(s):  
Genetic Diversity ◽  
Pinus Sylvestris ◽  
Scots Pine ◽  
Microsatellite Dna ◽  
Demographic History ◽  
Wood Quality ◽  
Breeding Value ◽  
Needle Length ◽  
Chloroplast Microsatellite ◽  
Forest District

Abstract Scots pine (Pinus sylvestris L.) is one of the most widespread forest trees in the world, ranging from southern Mediterranean mountains to eastern Siberia. 30 trees of Scots pine from the Miłomłyn Forest District were analyzed in terms of 7 nuclear and 8 chloroplast microsatellite DNA loci as well as 7 morphological needle traits. Nuclear microsatellites (Simple Sequence Repeats) have proved to be useful in studying phylogeographic and gene flow patterns in conifers and are being used to infer the demographic history of tree species. A population’s genetic diversity, in fact, represents an important criterion which could be applied in planning future forest management and breeding. Additionally, many elements of Scots pine morphology, including needle traits, are subject to environmental modification. However, the adaptability of those traits remains, to some extent, under genetic control. The trees we investigated here are called Tabórz pine located in the northeast of Poland. This population is described as one of the best in Poland and even in Europe regarding bio mass production, ductility and wood quality. The aim of the study was to determine variation and genetic structure within this population. An analysis of phenotypic differentiation showed that the traits which differed the most between individuals were needle length, the number of serrations per 2 mm of needle length along the right edge and the number of stomatal rows on the convex side of the needles. In Tabórz pine, we detected 30 different genotypes with 87 alleles as well as 28 haplotypes with 30 alleles. We also revealed a slight excess of homozygotes, but the population is still in Hardy-Weinberg equilibrium. The Scots pine population from Tabórz furthermore showed a higher level of genetic diversity compared to stands from other Polish and European regions. This diversity may be the main factor impacting on the population’s wood quality and its breeding value.

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