scholarly journals Alternative Proteins as a Source of Bioactive Peptides: The Edible Snail and Generation of Hydrolysates Containing Peptides with Bioactive Potential for Use as Functional Foods

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 276
Author(s):  
Maria Hayes ◽  
Leticia Mora
Keyword(s):  
Protein Hydrolysate ◽  
Health Benefits ◽  
Positive Control ◽  
Bioactive Peptide ◽  
Protein Source ◽  
Edible Snail ◽  
Snail Species ◽  
Accession Number ◽  
Potential Health

Members of the Phylum Mollusca include shellfish such as oysters and squid but also the edible garden snail known as Helix aspersa. This snail species is consumed as a delicacy in countries including France (where they are known as petit-gris), southern Spain (where they are known as Bobe), Nigeria, Greece, Portugal and Italy but is not a traditional food in many other countries. However, it is considered an excellent protein source with a balanced amino acid profile and an environmentally friendly, sustainable protein source. The aim of this work was to develop a different dietary form of snail protein by generating protein hydrolysate ingredients from the edible snail using enzyme technology. A second aim was to assess the bioactive peptide content and potential health benefits of these hydrolysates. H. aspersa hydrolysates were made using the enzyme Alcalase® and the nutritional profile of these hydrolysates was determined. In addition, the bioactive peptide content of developed hydrolysates was identified using mass spectrometry. The potential heart health benefits of developed snail hydrolysates were measured in vitro using the Angiotensin-I-converting Enzyme (ACE-1; EC 3.4.15.1) inhibition assay, and the ACE-1 inhibitory drug Captopril© was used as a positive control. The generated H. aspersa hydrolysates were found to inhibit ACE-1 by 95.60% (±0.011) when assayed at a concentration of 1 mg/mL (n = 9) compared to the positive control Captopril© which inhibited ACE-1 by 96.53% (±0.0156) when assayed at a concentration of 0.005 mg/mL (n = 3). A total of 113 unique peptide sequences were identified following MS analysis with peptides identified ranging from 628.35 Da (peptide GGGLVGGI—protein accession number sp|P54334|XKDO_BACSU) to 2343.14 Da (peptide GPAGVPGLPGAKGDHGFPGSSGRRGD—protein accession number sp|Q7SIB2|CO4A1_BOVIN) in size using the BIOPEP-UWM database.

Composition and Potential Health Benefits of Pomegranate: A Review

2019 ◽  
Vol 25 (16) ◽  
pp. 1817-1827 ◽  
Author(s):  
Vesna Vučić ◽  
Milkica Grabež ◽  
Armen Trchounian ◽  
Aleksandra Arsić
Keyword(s):  
Current Knowledge ◽  
Health Benefits ◽  
Punica Granatum ◽  
In Vivo Studies ◽  
Full Potential ◽  
Fruit Peel ◽  
Potential Health ◽  
Comprehensive Search

Background:: Pomegranate (Punica granatum L.) fruits are widely consumed and used as preventive and therapeutic agents since ancient times. Pomegranate is a rich source of a variety of phytochemicals, which are responsible for its strong antioxidative and anti-inflammatory potential. Objective:: The aim of this review is to provide an up-to-date overview of the current knowledge of chemical structure and potential health benefits of pomegranate. Method: : A comprehensive search of available literature. Results:: The review of the literature confirms that juice and extracts obtained from different parts of this plant, including fruit peel, seeds, and leaves exert health benefits in both in vitro and in vivo studies. The antidiabetic, antihypertensive, antimicrobial and anti-tumour effects of pomegranate fruit are of particular scientific and clinical interest. Conclusion:: Further investigations are required to clarify the mechanism of action of the bioactive ingredients and to reveal full potential of pomegranate as both preventive and therapeutic agent.

scholarly journals The potential health benefits of legumes as a good source of dietary fibre

2009 ◽  
Vol 103 (4) ◽  
pp. 569-574 ◽  
Author(s):  
Trinidad P. Trinidad ◽  
Aida C. Mallillin ◽  
Anacleta S. Loyola ◽  
Rosario S. Sagum ◽  
Rosario R. Encabo
Keyword(s):  
Dietary Fibre ◽  
Human Subjects ◽  
Health Benefits ◽  
Scientific Basis ◽  
Good Source ◽  
Potential Health ◽  
Kidney Beans ◽  
Mineral Availability ◽  
Lima Beans

Dietary fibre has been shown to have important health implications in the prevention of risks of chronic diseases. The objective of the present study was to determine the potential health benefits of legumes as a good source of dietary fibre. Six to ten local legumes were studied as follows: cowpeas, mung beans, pole sitao, chickpeas, green peas, groundnuts, pigeon peas, kidney beans, lima beans and soyabeans. The following studies were conducted: (a) mineral availability, in vitro; (b) glycaemic index (GI) in non-diabetic and diabetic human subjects; (c) the cholesterol-lowering effect in human subjects with moderately raised serum cholesterol levels. The highest Fe availability among legumes was for lima beans (9·5 (sem 0·1)) while for Zn and Ca, the highest availability was for kidney beans (49·3 (sem 4·5)) and pigeon peas (75·1 (sem 7·1)), respectively. Groundnuts have the lowest Fe (1·3 (sem 1·1)), Zn (7·9 (sem 1·3)) and Ca (14·6 (sem 2·8)) availability. Legumes are low-GI foods ( < 55), ranging from 6 (chickpeas) to 13 (mung beans). Kidney beans showed significant reductions for both total (6 %) and LDL-cholesterol (9 %), and groundnuts for total cholesterol (7 %; P < 0·05). We conclude that mineral availability from legumes differs and may be attributed to their mineral content, mineral–mineral interaction and from their phytic and tannic acid content; legumes are considered low-GI foods and have shown potential hypocholesterolaemic effects. The above studies can be a scientific basis for considering legumes as functional foods.

scholarly journals Insects as a source of phenolic compounds and potential health benefits

2021 ◽  
pp. 1-12
Author(s):  
M.C. Nino ◽  
L. Reddivari ◽  
C. Osorio ◽  
I. Kaplan ◽  
A.M. Liceaga
Keyword(s):  
Phenolic Compounds ◽  
De Novo ◽  
Health Benefits ◽  
Antimicrobial Activities ◽  
In Vivo Studies ◽  
Plant Phenolics ◽  
Potential Health ◽  
Bioactive Potential

The use of insects in traditional medicine and unveiling the chemical structure of the bright pigments in butterfly wings led to the discovery of bioactive phenolic compounds in the insect bodies. These metabolites have been found not only due to the insect absorption and metabolisation of the plant-derived phenolic present in their diet, but also from the ability of insects to synthesise phenolic compounds de novo through the sclerotisation process. Plant phenolics are secondary metabolites involved in the protection of tissues against UV radiation, herbivores, and pathogens, as well as pigmentation of fruits and flowers. These bioactive compounds exhibit antioxidant, anti-inflammatory, anticancer, and antimicrobial activities, demonstrated through in vitro and in vivo studies. This bioactive potential is thought to occur due to their chemical characteristics that allow them to stabilise reactive oxygen species (ROS), chelate prooxidant metal ions, interact with key enzymes and signal cascades involved in biological pathways. Bioactivity of plant phenolics and both in vitro, in vivo studies, suggest that the dietary compounds absorbed by the insect maintain their chemical and bioactive properties. Further characterisation of the phenolic composition in edible insects and evaluation of their bioactive capacity as well as their bioavailability, could result in discovering additional health benefits of entomophagy apart from macro-nutritional (e.g. protein) content.

scholarly journals Potential Health Benefits of Origanum heracleoticum Essential Oil: Phytochemical and Biological Variability among Different Calabrian Populations

2018 ◽  
Vol 13 (9) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Mariangela Marrelli ◽  
Fabrizio Araniti ◽  
Maria Rosa Abenavoli ◽  
Giancarlo Statti ◽  
Filomena Conforti
Keyword(s):  
Essential Oils ◽  
Health Benefits ◽  
Hierarchical Cluster ◽  
Inhibitory Effect ◽  
Biological Properties ◽  
In Vitro Assays ◽  
No Production ◽  
Potential Health ◽  
Phytochemical Content

The potential health benefits of the essential oils of six different populations of Origanum heracleoticum L. from Calabria (Italy) were assessed, together with the evaluation of the influence of the site of collection on their metabolic profile and biological activity. Different terpenoids were identified, being thymol, carvacrol and o-cymene among the most abundant ones. Hierarchical cluster analysis allowed the identification of two main groups, which were discriminated by the altitude of collection. The antioxidant activity was tested by means of two in vitro assays: DPPH and β-carotene bleaching test. Samples EO-4 and EO-3 were the most active in protecting linoleic acid from peroxidation, with IC50 values of 4.00 and 4.68 μg/mL after 30 minutes of incubation. The anti-inflammatory potential was assessed through the evaluation of the capacity to inhibit NO production in lipopolysaccharide (LPS)-induced murine macrophage RAW 264.7 cell line. All essential oils induced a dose-depending inhibitory effect. An excellent activity was demonstrated for sample EO-4, followed by sample EO-3 (IC50 values equal to 32.77 and 49.48 μg/mL, respectively). Observed biological properties were correlated to the phytochemical content of analyzed samples.

Acacia (SEN) SupergumTM (Gum Arabic): in vivo and in vitro Evaluation of Potential Health Benefits in Renal Disease

2011 ◽  
pp. 291-300
Author(s):  
D.A Glover ◽  
N. Matsumoto ◽  
S.G Riley ◽  
T. Wolever ◽  
K. Ushida ◽  
...  
Keyword(s):  
Renal Disease ◽  
Health Benefits ◽  
Gum Arabic ◽  
Potential Health

scholarly journals Discovering Health Benefits of Phytochemicals with Integrated Analysis of the Molecular Network, Chemical Properties and Ethnopharmacological Evidence

Nutrients ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 1042 ◽  
Author(s):  
Sunyong Yoo ◽  
Kwansoo Kim ◽  
Hojung Nam ◽  
Doheon Lee
Keyword(s):  
Health Effects ◽  
Molecular Analysis ◽  
In Silico ◽  
Health Benefits ◽  
Chemical Properties ◽  
In Silico Analysis ◽  
Integrated Analysis ◽  
Screening Methods ◽  
Potential Health

Identifying the health benefits of phytochemicals is an essential step in drug and functional food development. While many in vitro screening methods have been developed to identify the health effects of phytochemicals, there is still room for improvement because of high cost and low productivity. Therefore, researchers have alternatively proposed in silico methods, primarily based on three types of approaches; utilizing molecular, chemical or ethnopharmacological information. Although each approach has its own strength in analyzing the characteristics of phytochemicals, previous studies have not considered them all together. Here, we apply an integrated in silico analysis to identify the potential health benefits of phytochemicals based on molecular analysis and chemical properties as well as ethnopharmacological evidence. From the molecular analysis, we found an average of 415.6 health effects for 591 phytochemicals. We further investigated ethnopharmacological evidence of phytochemicals and found that on average 129.1 (31%) of the predicted health effects had ethnopharmacological evidence. Lastly, we investigated chemical properties to confirm whether they are orally bio-available, drug available or effective on certain tissues. The evaluation results indicate that the health effects can be predicted more accurately by cooperatively considering the molecular analysis, chemical properties and ethnopharmacological evidence.

scholarly journals Nutritional properties of selected superfood extracts and their potential health benefits

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12525
Author(s):  
Jacqueline P. Barsby ◽  
James M. Cowley ◽  
Shalem Y. Leemaqz ◽  
Jessica A. Grieger ◽  
Daniel R. McKeating ◽  
...  
Keyword(s):  
Cell Viability ◽  
Antioxidant Properties ◽  
Health Benefits ◽  
Intestinal Epithelial ◽  
Omega 3 ◽  
Nutritional Properties ◽  
Potential Health ◽  
Gastrointestinal Health ◽  
Chia Seed

Background The term ‘superfoods’ is used to market foods considered to have significant health benefits. ‘Superfoods’ are claimed to prevent diseases as well as improving overall health, though the lack of explicit criteria means that any food can be labelled ‘super’ without support from scientific research. Typically, these ‘superfoods’ are rich in a particular nutrient for example antioxidants or omega-3 fatty acids. The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits. Methods We developed a simple aqueous extraction method for ground seeds and analysed their composition by mineral, protein and monosaccharide analyses. Cell viability assays were performed on Caco-2 and IEC-6 intestinal epithelial cells using increasing doses of the prepared extracts. Results Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05). Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity. However, differences in extract composition and antioxidant properties could not be directly related to the observed increase in cell viability suggesting that other components in the extracts may be responsible. Future studies will further characterize these extracts and investigate whether they are beneficial for gastrointestinal health.

scholarly journals Orange thyme: phytochemical profiling, in vitro bioactivities of extracts and potential health benefits

2021 ◽  
pp. 100171
Author(s):  
Amélia M. Silva ◽  
Luís M. Félix ◽  
Isabel Teixeira ◽  
Carlos Martins-Gomes ◽  
Judith Schäfer ◽  
...  
Keyword(s):  
Health Benefits ◽  
Potential Health

scholarly journals Food Applications and Potential Health Benefits of Pomegranate and its Derivatives

Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 122 ◽  
Author(s):  
Panagiotis Kandylis ◽  
Evangelos Kokkinomagoulos
Keyword(s):  
Health Benefits ◽  
Punica Granatum ◽  
Physicochemical Characteristics ◽  
In Vivo Studies ◽  
Protective Agent ◽  
Potential Health ◽  
Food Applications ◽  
Punica Granatum L

Pomegranate (Punica granatum L.) is an ancient fruit that is particularly cultivated in west Asia, though it is also cultivated in the Mediterranean region and other parts of the world. Since ancient years, its consumption has been associated with numerous health benefits. In recent years, several in vitro and in vivo studies have revealed its beneficial physiological activities, especially its antioxidative, antimicrobial and anti-inflammatory properties. Furthermore, human-based studies have shown promising results and have indicated pomegranate potential as a protective agent of several diseases. Following that trend and the food industry’s demand for antioxidants and antimicrobials from natural sources, the application of pomegranate and its extracts (mainly as antioxidants and antimicrobials), has been studied extensively in different types of food products with satisfactory results. This review aims to present all the recent studies and trends in the applications of pomegranate in the food industry and how these trends have affected product’s physicochemical characteristics and shelf-life. In addition, recent in vitro and in vivo studies are presented in order to reveal pomegranate’s potential in the treatment of several diseases.

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