Alternative Proteins as a Source of Bioactive Peptides: The Edible Snail and Generation of Hydrolysates Containing Peptides with Bioactive Potential for Use as Functional Foods

Members of the Phylum Mollusca include shellfish such as oysters and squid but also the edible garden snail known as Helix aspersa. This snail species is consumed as a delicacy in countries including France (where they are known as petit-gris), southern Spain (where they are known as Bobe), Nigeria, Greece, Portugal and Italy but is not a traditional food in many other countries. However, it is considered an excellent protein source with a balanced amino acid profile and an environmentally friendly, sustainable protein source. The aim of this work was to develop a different dietary form of snail protein by generating protein hydrolysate ingredients from the edible snail using enzyme technology. A second aim was to assess the bioactive peptide content and potential health benefits of these hydrolysates. H. aspersa hydrolysates were made using the enzyme Alcalase® and the nutritional profile of these hydrolysates was determined. In addition, the bioactive peptide content of developed hydrolysates was identified using mass spectrometry. The potential heart health benefits of developed snail hydrolysates were measured in vitro using the Angiotensin-I-converting Enzyme (ACE-1; EC 3.4.15.1) inhibition assay, and the ACE-1 inhibitory drug Captopril© was used as a positive control. The generated H. aspersa hydrolysates were found to inhibit ACE-1 by 95.60% (±0.011) when assayed at a concentration of 1 mg/mL (n = 9) compared to the positive control Captopril© which inhibited ACE-1 by 96.53% (±0.0156) when assayed at a concentration of 0.005 mg/mL (n = 3). A total of 113 unique peptide sequences were identified following MS analysis with peptides identified ranging from 628.35 Da (peptide GGGLVGGI—protein accession number sp|P54334|XKDO_BACSU) to 2343.14 Da (peptide GPAGVPGLPGAKGDHGFPGSSGRRGD—protein accession number sp|Q7SIB2|CO4A1_BOVIN) in size using the BIOPEP-UWM database.

Pengaruh pemberian berbagai jenis keong terhadap pertumbuhan dan kelangsungan hidup ikan patin (Pangasius pangasius)

AbstrakIkan Patin (Pangasius pangasius) merupakan ikan air tawar yang memiliki nilai ekonomis yang tinggi. Untuk membantu mempercepat pertumbuhan selain dengan menggunakan pellet, ikan patin juga dapat diberikan pakan alami seperti keong mas (Pomacea canaliculata Lamarck), bekicot (Achatina fulica) dan siput air tawar (Lemnaea javanica). Penelitian ini bertujuan untuk mengetahui pengaruh pemberian pakan jenis keong-keongan yang berbeda terhadap pertumbuhan, kelangsungan hidup, dan konversi pakan. Hasil penelitian menunjukkan bahwa pertumbuhan terbaik terdapat pada perlakuan A, yaitu dengan pemberian keong mas. terbaik kedua pada perlakuan B (pemberian bekicot) dan yang paling rendah pada perlakuan C (pemberian keong air tawar). Selanjutnya untuk tingkat kelangsungan hidup terbaik terdapat pada perlakuan A dan B yaitu sebesar 95.24%, dan yang terendah perlakuan C sebesar 90.47%. Rasio konversi pakan pada perlakuan A yaitu 3.45, sedangkan pada perlakuan C sebesar 7.44.Kata kunci: ikan patin; keong mas; bekicot, siput air tawar; pertumbuhanAbstractPangasius catfish (Pangasius pangasius) is a freshwater fish that has high economic value. To accelerate growth in addition by using pellet, this catfish can also be given live food such as the golden snail (Pomacea canaliculata Lamarck), edible snail (Achatina fulica), and freshwater snail (Lemnaea javanica). This study aims to determine the effect of different types of snail feeding on growth, survival rate, and feed conversion ratio. The results showed that the best growth was found in treatment A, by giving the golden snail, the second best growth in treatment B (by giving edible snail) and the lowest growth in treatment C (by giving freshwater snail). Furthermore, the best survival rate is in treatment A and B which is equal to 95.24%, and the lowest in treatment C (90.47%). Feed conversion ratio in treatment A was 3.45, while in treatment C was 7.44.Keywords: Pangasius catfish; The golden snail; edible snail; freshwater snail; growth