scholarly journals Root-Specific Expression of a Jacalin Lectin Family Protein Gene Requires a Transposable Element Sequence in the Promoter

Genes ◽  
2018 ◽  
Vol 9 (11) ◽  
pp. 550 ◽  
Author(s):  
Qiong Wu ◽  
Neil Smith ◽  
Daai Zhang ◽  
Changyong Zhou ◽  
Ming-Bo Wang
Keyword(s):  
Expression Pattern ◽  
Plant Defence ◽  
Plant Genome ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Family Protein ◽  
Lectin Family ◽  
Dna Demethylase

Transposable elements (TEs) are widespread in the plant genome and can impact on the expression of neighbouring genes. Our previous studies have identified a number of DNA demethylase-regulated defence-related genes that contain TE sequences in the promoter and show tissue-specific expression in Arabidopsis. In this study we investigated the role of the promoter TE insertions in the root-specific expression of a DNA demethylase-regulated gene, AT5G38550, encoding a Jacalin lectin family protein. Using a promoter:GUS fusion reporter gene approach, we first demonstrated that the full-length promoter fragment, carrying four TE sequences, contained the essential regulatory information required for root-specific expression and DNA demethylase regulation in Arabidopsis. By successive deletion of the four TE sequences, we showed that one of the four TE insertions, a 201-bp TE fragment of the hAT DNA transposon family, was required for root-specific expression: Deletion of this TE, but not the first two TE sequences, converted the root-specific expression pattern to a constitutive expression pattern in Arabidopsis plants. Our study provides an example indicating an important role of TE insertions in tissue-specific expression of plant defence-related genes.

scholarly journals Tissue Distribution of ACE2 Protein in Syrian Golden Hamster (Mesocricetus auratus) and Its Possible Implications in SARS-CoV-2 Related Studies

2021 ◽  
Vol 11 ◽  
Author(s):  
Voddu Suresh ◽  
Deepti Parida ◽  
Aliva P. Minz ◽  
Manisha Sethi ◽  
Bhabani S. Sahoo ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Golden Hamster ◽  
Expression Patterns ◽  
Mesocricetus Auratus ◽  
Syrian Golden Hamster ◽  
Surface Epithelium ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Specific Expression Pattern

The Syrian golden hamster (Mesocricetus auratus) has recently been demonstrated as a clinically relevant animal model for SARS-CoV-2 infection. However, lack of knowledge about the tissue-specific expression pattern of various proteins in these animals and the unavailability of reagents like antibodies against this species hampers these models’ optimal use. The major objective of our current study was to analyze the tissue-specific expression pattern of angiotensin-converting enzyme 2, a proven functional receptor for SARS-CoV-2 in different organs of the hamster. Using two different antibodies (MA5-32307 and AF933), we have conducted immunoblotting, immunohistochemistry, and immunofluorescence analysis to evaluate the ACE2 expression in different tissues of the hamster. Further, at the mRNA level, the expression of Ace2 in tissues was evaluated through RT-qPCR analysis. Both the antibodies detected expression of ACE2 in kidney, small intestine, tongue, and liver. Epithelium of proximal tubules of kidney and surface epithelium of ileum expresses a very high amount of this protein. Surprisingly, analysis of stained tissue sections showed no detectable expression of ACE2 in the lung or tracheal epithelial cells. Similarly, all parts of the large intestine were negative for ACE2 expression. Analysis of tissues from different age groups and sex didn’t show any obvious difference in ACE2 expression pattern or level. Together, our findings corroborate some of the earlier reports related to ACE2 expression patterns in human tissues and contradict others. We believe that this study’s findings have provided evidence that demands further investigation to understand the predominant respiratory pathology of SARS-CoV-2 infection and disease.

scholarly journals Chicken Pleiotrophin: Regulation of Tissue Specific Expression by Estrogen in the Oviduct and Distinct Expression Pattern in the Ovarian Carcinomas

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e34215 ◽  
Author(s):  
Jin-Young Lee ◽  
Wooyoung Jeong ◽  
Whasun Lim ◽  
Jinyoung Kim ◽  
Fuller W. Bazer ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Ovarian Carcinomas ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

scholarly journals Searching for Tissue-Specific Expression Pattern-Linked Nucleotides of UGT1A Isoforms

PLoS ONE ◽  
2007 ◽  
Vol 2 (4) ◽  
pp. e396 ◽  
Author(s):  
Wei Zhang ◽  
Wanqing Liu ◽  
Federico Innocenti ◽  
Mark J. Ratain
Keyword(s):  
Expression Pattern ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Specific Expression Pattern

scholarly journals The elusive MAESTRO gene: Its human reproductive tissue-specific expression pattern

PLoS ONE ◽  
2017 ◽  
Vol 12 (4) ◽  
pp. e0174873
Author(s):  
Shlomit Kenigsberg ◽  
Patricia D. A. Lima ◽  
Leila Maghen ◽  
Brandon A. Wyse ◽  
Chantal Lackan ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Reproductive Tissue ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Human Reproductive ◽  
Specific Expression Pattern

scholarly journals Role of DNA methylation in the tissue-specific expression of the CYP17A1 gene for steroidogenesis in rodents

2009 ◽  
Vol 202 (1) ◽  
pp. 99-109 ◽  
Author(s):  
Elika Missaghian ◽  
Petra Kempná ◽  
Bernhard Dick ◽  
Andrea Hirsch ◽  
Rasoul Alikhani-Koupaei ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Histone Deacetylase Inhibitors ◽  
Cpg Island ◽  
Specific Expression ◽  
Tissue Specific ◽  
Adrenal Cells ◽  
Tissue Specific Expression ◽  
As Species ◽  
Species Specific

The CYP17A1 gene is the qualitative regulator of steroidogenesis. Depending on the presence or absence of CYP17 activities mineralocorticoids, glucocorticoids or adrenal androgens are produced. The expression of the CYP17A1 gene is tissue as well as species-specific. In contrast to humans, adrenals of rodents do not express the CYP17A1 gene and have therefore no P450c17 enzyme for cortisol production, but produce corticosterone. DNA methylation is involved in the tissue-specific silencing of the CYP17A1 gene in human placental JEG-3 cells. We investigated the role of DNA methylation for the tissue-specific expression of the CYP17A1 gene in rodents. Rats treated with the methyltransferase inhibitor 5-aza-deoxycytidine excreted the cortisol metabolite tetrahydrocortisol in their urine suggesting that treatment induced CYP17 expression and 17α-hydroxylase activity through demethylation. Accordingly, bisulfite modification experiments identified a methylated CpG island in the CYP17 promoter in DNA extracted from rat adrenals but not from testes. Both methyltransferase and histone deacetylase inhibitors induced the expression of the CYP17A1 gene in mouse adrenocortical Y1 cells which normally do not express CYP17, indicating that the expression of the mouse CYP17A1 gene is epigenetically controlled. The role of DNA methylation for CYP17 expression was further underlined by the finding that a reporter construct driven by the mouse −1041 bp CYP17 promoter was active in Y1 cells, thus excluding the lack of essential transcription factors for CYP17 expression in these adrenal cells.

Sign in / Sign up

Export Citation Format

Share Document