scholarly journals Evaluation of DNA Methylation Changes and Micronuclei in Workers Exposed to a Construction Environment

2019 ◽  
Vol 16 (6) ◽  
pp. 902 ◽  
Author(s):  
Isana Silva ◽  
Manoela Ramos ◽  
Lídia Arantes ◽  
André Lengert ◽  
Marco Oliveira ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Particulate Matter ◽  
Repetitive Sequences ◽  
Promoter Regions ◽  
Exposed Workers ◽  
Polycyclic Aromatic ◽  
Group 2 ◽  
Environmental Air ◽  
The Relationship ◽  
Group 1

Methylation levels in tumor-suppressor genes and repetitive sequences have previously been used to study the relationship between environmental air pollution and epigenetic changes related to cancer. In this study, we measured the methylation profiles of the promoter regions CDKN2A, MLH1 and APC and the repetitive sequence LINE-1 in 59 workers exposed to the construction environment and in 49 unexposed workers. We also evaluated the micronuclei frequency and levels of trace elements in the blood of all workers. We evaluated of levels of particulate matter and polycyclic aromatic hydrocarbons (PAHs) at the construction site to characterize the environmental exposure. Our findings demonstrated that exposed workers exhibited significantly higher average levels of promoter methylation of CDKN2A, APC, and MLH1 genes and increased hypomethylation of the LINE-1 in comparison to unexposed workers (all p < 0.05). A higher frequency of micronuclei was observed in the exposed group (2 ± 2) compared to the unexposed group (1 ± 1) with p < 0.001. High levels of particulate matter (51–841 μg/m3) and some PAHs were found in samples from the construction environment. In summary, we provide evidence of increased DNA damage and altered DNA methylation of exposed workers, suggesting that genomic approaches to biomonitoring may be an effective way of estimating future cancer risk for construction workers.

The Relationship between Serum Ferritin Levels and 5-Year All-Cause Mortality in Hemodialysis Patients

2021 ◽  
pp. 1-7
Author(s):  
Emre Erdem ◽  
Ahmet Karatas ◽  
Tevfik Ecder
Keyword(s):  
Serum Ferritin ◽  
Hemodialysis Patients ◽  
Rank Test ◽  
Significant Difference ◽  
All Cause Mortality ◽  
Group 3 ◽  
Group 2 ◽  
The Relationship ◽  
Group 1

<b><i>Introduction:</i></b> The effect of high serum ferritin levels on long-term mortality in hemodialysis patients is unknown. The relationship between serum ferritin levels and 5-year all-cause mortality in hemodialysis patients was investigated in this study. <b><i>Methods:</i></b> A total of 173 prevalent hemodialysis patients were included in this study. The patients were followed for up to 5 years and divided into 3 groups according to time-averaged serum ferritin levels (group 1: serum ferritin &#x3c;800 ng/mL, group 2: serum ferritin 800–1,500 ng/mL, and group 3: serum ferritin &#x3e;1,500 ng/mL). Along with the serum ferritin levels, other clinical and laboratory variables that may affect mortality were also included in the Cox proportional-hazards regression analysis. <b><i>Results:</i></b> Eighty-one (47%) patients died during the 5-year follow-up period. The median follow-up time was 38 (17.5–60) months. The 5-year survival rates of groups 1, 2, and 3 were 44, 64, and 27%, respectively. In group 3, the survival was lower than in groups 1 and 2 (log-rank test, <i>p</i> = 0.002). In group 1, the mortality was significantly lower than in group 3 (HR [95% CI]: 0.16 [0.05–0.49]; <i>p</i> = 0.001). In group 2, the mortality was also lower than in group 3 (HR [95% CI]: 0.32 [0.12–0.88]; <i>p</i> = 0.026). No significant difference in mortality between groups 1 and 2 was found (HR [95% CI]: 0.49 [0.23–1.04]; <i>p</i> = 0.063). <b><i>Conclusion:</i></b> Time-averaged serum ferritin levels &#x3e;1,500 ng/mL in hemodialysis patients are associated with an increased 5-year all-cause mortality risk.

scholarly journals Factors associated with rs1801394 of the methionine synthase reductase gene polymorphism in patients with rheumatoid arthritis

2021 ◽  
Vol 15 (3) ◽  
pp. 15-19
Author(s):  
M. Yu. Krylov ◽  
G. I. Gridneva ◽  
Yu. V. Muravyev
Keyword(s):  
Rheumatoid Arthritis ◽  
Clinical Characteristics ◽  
Methionine Synthase ◽  
Nucleotide Polymorphisms ◽  
Allelic Discrimination ◽  
Methionine Synthase Reductase ◽  
Reductase Gene ◽  
Group 2 ◽  
The Relationship ◽  
Group 1

Clinical response to methotrexate (MT) therapy in rheumatoid arthritis (RA) can be predicted on the basis of some single nucleotide polymorphisms (SNPs) of genes, involved in folate metabolism. One of these SNPs is the rs1801394 (A66G) polymorphism of the methionine synthase reductase gene (MTRR). We investigated the association of this polymorphism with the clinical characteristics of RA patients after 6 months of MT therapy. Studies of the relationship between the response to MT therapy and the rs1801394 polymorphism have not been carried out in Russia previously.Objective: to study the possible association of the rs1801394 polymorphism with the clinical characteristics of patients with RA after 6 months of MT therapy.Patients and methods. The study included 60 patients with RA who met the ACR / EULAR criteria (2010) and received≥20 mg MT per week continuously. Based on the EULAR criteria, patients were divided into two groups: group 1 (n=30) with a good (DAS28>1.2) and group 2 (n=30) with an unsatisfactory (DAS28 <1.2) response to MT therapy. Genotyping of the rs1801394 polymorphism was performed by allelic discrimination using real-time polymerase chain reaction.Results and discussion. The frequency distribution of the A66G polymorphism genotypes in both groups was similar, however, in the 2nd group with an unsatisfactory response, there was a tendency towards a higher frequency of the mutant GG genotype (p=0.067). An association of the A66G polymorphism with gender and disease duration was found. In group 1, the AG genotype was more often detected in men than in women (p=0.017). In group 2, the AG genotype was also more common in men (p=0.075). In addition, in this group, carriers of the G allele (genotypes AG and GG) had a longer duration of the disease than carriers of the AA genotype (p=0.003 and p=0.005, respectively).Conclusion. In the present study, the relationship of the studied polymorphism rs1801394 of the MTRR gene with gender and duration of RA disease was established.

scholarly journals Inverse Relationship between Adenoid Size and Asthma or Atopy in Children: A Preliminary Study

2020 ◽  
Vol 63 (9) ◽  
pp. 409-414
Author(s):  
Yangseop Noh ◽  
Ji-Eun Choi ◽  
Kyung Eun Lee ◽  
Seung-Kyu Chung ◽  
Sang Duk Hong ◽  
...  
Keyword(s):  
Medical Center ◽  
Allergic Sensitization ◽  
Allergic Diseases ◽  
Adenoid Hypertrophy ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Diagnosis Criteria ◽  
The Relationship ◽  
Group 1

Background and Objectives One of the most common surgical procedures in children is adenoidectomy, but the causes of adenoid hypertrophy are not fully understood. Some studies have found that allergies can be a risk factor for adenoid hypertrophy, asthma being one of these allergic diseases. This study aimed to investigate the relationship between adenoid size and asthma in a group of children.Subjects and Method This study reviewed a total of 2063 pediatric patients with or without atopy and asthma who visited the Otorhinolaryngology and Pediatric unit at a tertiary medical center from January 2011 to June 2016. We classified these patients into 4 groups according to the presence of asthma or atopy and randomly selected 100 patients from each group (to a total of 400 pts): group 1 (asthma-, atopy-); group 2 (asthma-, atopy+); group 3 (asthma+, atopy-) and group 4 (asthma+, atopy+). The presence of allergic sensitization (atopy) was evaluated by CAP test and total IgE. Asthma was diagnosed according to the diagnosis criteria in the Korean guideline for asthma. Adenoid size was evaluated with the adenoidal-nasopharyngeal ratio (A/N ratio) by the adenoid view.Results The four groups did not differ from each other significantly in age or sex. There was a negative correlation between adenoid size and atopic and asthmatic condition. Group 1 had a significantly larger A/N ratio than the other groups (group 1=0.534±0.138; group 2=0.469± 0.140; group 3=0.476±0.135; group 4=0.482±0.128, <i>p</i><0.05). However, group 4 showed nearly identical results to groups 2 and 3, despite the combination of asthma and atopy in group 4.Conclusion Large adenoids were negatively associated with atopy and asthma. This finding may be explained by a decrease in adenoid stimulation by nasal obstruction and a difference in the immune system, including allergic immune reactions. Further studies are needed.

Motor cycles with phase III in antrum are associated with high motilin levels and prolonged gallbladder emptying

1993 ◽  
Vol 264 (4) ◽  
pp. G596-G600 ◽  
Author(s):  
M. F. Stolk ◽  
K. J. van Erpecum ◽  
A. J. Smout ◽  
L. M. Akkermans ◽  
J. B. Jansen ◽  
...  
Keyword(s):  
Bile Acid ◽  
Cycle Length ◽  
Phase Iii ◽  
Gallbladder Motility ◽  
Gallbladder Emptying ◽  
Motor Complex ◽  
Group 2 ◽  
Relationship Of ◽  
The Relationship ◽  
Group 1

We examined the relationship of interdigestive gallbladder emptying with the different phases of the migrating motor complex (MMC) and with plasma levels of cholecystokinin (CCK) and motilin. In 10 volunteers 20 cycles of the MMC were recorded. In 11 cycles phase III occurred in antrum and duodenum (group 1). In nine cycles phase III was observed only in duodenum (group 2). In group 1 gallbladder emptying started at 30% of total cycle length and continued until the end of the cycle. Maximal gallbladder emptying was 33.3 +/- 3.3% (SE). In group 2 gallbladder emptying also started at 30% of total cycle length but ended at 60%. Maximal gallbladder emptying in this group was 24.3 +/- 3.1% (P < 0.05). Motilin levels were higher in group 1 than in group 2 during phase IIB (240.1 +/- 28.5 and 142.1 +/- 30.9 pg/ml, respectively, P < 0.05) and during phase III (210.8 +/- 24.3 and 93.5 +/- 12.5 pg/ml, respectively, P < 0.05). We conclude that: 1) phase III activities starting in the antrum are preceded by greater and prolonged gallbladder emptying, higher motilin levels, and higher intraduodenal bile acid concentrations than phase III activities starting in the duodenum and 2) no relationship between interdigestive gallbladder motility and CCK levels could be demonstrated.

scholarly journals Interaction Between Age and Change in Velocity During a Baseball Training Program

2020 ◽  
Vol 8 (6) ◽  
pp. 232596712092793
Author(s):  
Christopher Antonacci ◽  
Thomas R. Atlee ◽  
Peter N. Chalmers ◽  
Christopher Hadley ◽  
Meghan E. Bishop ◽  
...  
Keyword(s):  
Training Program ◽  
Age Groups ◽  
Maximum Velocity ◽  
Age Group ◽  
Significant Difference ◽  
Group 3 ◽  
Baseball Pitchers ◽  
Group 2 ◽  
The Relationship ◽  
Group 1

Background: Pitching velocity is one of the most important metrics used to evaluate a baseball pitcher’s effectiveness. The relationship between age and pitching velocity after a lighter ball baseball training program has not been determined. Purpose/Hypothesis: The purpose of this study was to examine the relationship between age and pitching velocity after a lighter ball baseball training program. We hypothesized that pitching velocity would significantly increase in all adolescent age groups after a lighter baseball training program, without a significant difference in magnitude of increase based on age. Study Design: Cohort study; Level of evidence, 2. Methods: Baseball pitchers aged 10 to 17 years who completed a 15-week training program focused on pitching mechanics and velocity improvement were included in this study. Pitchers were split into 3 groups based on age (group 1, 10-12 years; group 2, 13-14 years; group 3, 15-17 years), and each group trained independently. Pitch velocity was assessed at 4 time points (sessions 3, 10, 17, and 25). Mean, maximum, and mean change in pitch velocity between sessions were compared by age group. Results: A total of 32 male baseball pitchers were included in the analysis. Mean/maximum velocity increased in all 3 age groups: 3.4/4.8 mph in group 1, 5.3/5.5 mph in group 2, and 5.3/5.2 mph in group 3. While mean percentage change in pitch velocity increased in all 3 age groups (group 1, 6.5%; group 2, 8.3%; group 3, 7.6%), the magnitude of change was not significantly different among age groups. Program session number had a significant effect on mean and maximum velocity, with higher mean and maximum velocity seen at later sessions in the training program ( P = .018). There was no interaction between age and program session within either mean or maximum velocity ( P = .316 and .572, respectively). Conclusion: Age had no significant effect on the magnitude of increase in maximum or mean baseball pitch velocity during a velocity and mechanics training program in adolescent males.

scholarly journals A Strategic Approach to Implementation of Medical Mentorship Programs

2016 ◽  
Vol 8 (1) ◽  
pp. 68-73 ◽  
Author(s):  
Thomas J. Caruso ◽  
Diane H. Steinberg ◽  
Nancy Piro ◽  
Kimberly Walker ◽  
Rebecca Blankenburg ◽  
...  
Keyword(s):  
Training Programs ◽  
Psychosocial Support ◽  
Control Group ◽  
Chi Square ◽  
Mentorship Program ◽  
Professional Aspirations ◽  
Group 2 ◽  
Survey Responses ◽  
The Relationship ◽  
Group 1

ABSTRACT Background Mentors influence medical trainees' experiences through career enhancement and psychosocial support, yet some trainees never receive benefits from involved mentors. Objective Our goals were to examine the effectiveness of 2 interventions aimed at increasing the number of mentors in training programs, and to assess group differences in mentor effectiveness, the relationship between trainees' satisfaction with their programs given the presence of mentors, and the relationship between the number of trainees with mentors and postgraduate year (PGY). Methods In group 1, a physician adviser funded by the graduate medical education department implemented mentorships in 6 residency programs, while group 2 involved a training program with funded physician mentoring time. The remaining 89 training programs served as controls. Chi-square tests were used to determine differences. Results Survey responses from group 1, group 2, and controls were 47 of 84 (56%), 34 of 78 (44%), and 471 of 981 (48%, P = .38), respectively. The percentages of trainees reporting a mentor in group 1, group 2, and the control group were 89%, 97%, and 79%, respectively (P = .01). There were no differences in mentor effectiveness between groups. Mentored trainees were more likely to be satisfied with their programs (P = .01) and to report that faculty supported their professional aspirations (P = .001). Across all programs, fewer first-year trainees (59%) identified a mentor compared to PGY-2 through PGY-8 trainees (84%, P &lt; .001). Conclusions A supported mentorship program is an effective way to create an educational environment that maximizes trainees' perceptions of mentorship and satisfaction with their training programs.

‘I am not at risk – typical tourists are’! Social comparison of risk in tourists

2011 ◽  
Vol 131 (6) ◽  
pp. 275-279 ◽  
Author(s):  
Svein Larsen ◽  
Wibecke Brun
Keyword(s):  
Cognitive Processes ◽  
Social Cognitive ◽  
Home Country ◽  
Optimistic Bias ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Not At Risk ◽  
The Relationship ◽  
Group 1

Aim: The presented research aims to study the relationship between judgements of risks to oneself as a tourist as compared to risks to ‘typical tourists’, ‘average tourists’ and ‘typical tourist from your home country’. Methods: Altogether, 1,892 tourists visiting Norway (summer 2010) filled in a questionnaire on aspects of being a tourist. Respondents were randomly assigned to one of four groups. Group 1 was asked about risk to themselves; Group 2 was asked to indicate their thoughts about ‘risk to typical tourists’; Group 3 indicated risks for ‘average tourists’; and Group 4 was asked about risks for typical tourists from their home country. Results: Results show that all risks were judged to be low, but significantly higher risks were attributed to the typical and average tourist than to ‘self’. Conclusions: The results are interpreted in terms of social cognitive processes such as the ‘optimistic bias’.

scholarly journals Relation between Blood Pressure Variability within a Single Visit and Stroke

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Wei Ma ◽  
Ying Yang ◽  
Litong Qi ◽  
Baowei Zhang ◽  
Lei Meng ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Blood Pressure Variability ◽  
Disease Risk ◽  
Cardiovascular Disease Risk ◽  
Single Visit ◽  
Group 3 ◽  
The Mean ◽  
Group 2 ◽  
The Relationship ◽  
Group 1

Blood pressure variability (BPV) has been identified as an important risk factor for cardiovascular events. The white coat effect (WCE), which is measured as the first systolic blood pressure (SBP) measurement minus the mean of the second and third measurements, is a BPV indicator within a single visit. In total, 2,972 participants who had three measurements of BP within a single visit were included. The participants were divided into three groups based on their WCE percentiles: Group 1 (WCE2.5-97.5, 2.5–97.5th percentiles of WCE), Group 2 (WCE2.5, 0–2.4th percentiles of WCE), and Group 3 (WCE97.5, 97.6–100th percentiles of WCE). A multiple logistic regression model was used to analyze the relationship between WCE and stroke after adjusting for cardiovascular disease risk factors. Compared with the WCE2.5-97.5 group, the OR for stroke in the WCE2.5 group was 2.78 (95% CI: 1.22, 6.36, p = 0.015 ). After adjusting for cardiovascular factors, OR increased to 3.12 (95% CI: 1.22, 7.96, p = 0.017 ). The OR of WCE for stroke was 0.93 (95%CI: 0.87, 0.99, p = 0.036 ). BPV within a single visit is associated with stroke. The value and direction of the change may be important as well.

scholarly journals Periodontal Status in Chronic Kidney Disease Patients

2017 ◽  
Vol 1 ◽  
Author(s):  
Lam Do Ai Nguyen ◽  
Thao Thi Thu Nguyen ◽  
Thuy Anh Vu Pham
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Multiple Logistic Regression Analysis ◽  
Cross Sectional Study ◽  
Related Factors ◽  
Cross Sectional ◽  
Periodontal Status ◽  
Group 2 ◽  
The Relationship ◽  
Group 1

<div><p class="AbstractTitle"><strong>Objectives:</strong> To compare periodontal status of chronic kidney disease patients with those in non-chronic kidney disease patients and to explore the relationship between periodontitis and chronic kidney disease in a group of Vietnamese.</p></div><p class="AbstractContent"><strong>Methods:</strong> A cross sectional study was conducted on 240 adults including 120 chronic kidney disease patients (group 1) and 120non-chronic kidney disease patients (group 2). The socio-demographic characteristic, dental and smoking habits were investigated by the questionnaire. Periodontal status (PPD, CAL, BOP) were examined. The periodontal status was compared between 2 groups. Multiple logistic regression analysis was performed to examine the relationship between chronic kidney disease and periodontitis after adjusting related factors.</p><p class="AbstractContent"><strong>Results</strong>: The clinically periodontal parameters in group 1 were all higher compared to those in group 2 (p&lt;0.001). The OR for periodontitis in patients who with age more than 60 years or brushed their teeth less than 2 times per day or had no scaling or in current smokers or with chronic kidney disease were 1.93; 4.27; 7.95; 4.21; 2.94 compared to counterparts, respectively (p&lt;0.05).</p><p class="AbstractContent"><strong>Conclusion:</strong> The periodontal status of chronic kidney disease patients was found worse than those in non-chronic kidney disease patients. The chronic kidney disease was significantly related to periodontitis.</p>

scholarly journals Expression of Sigma-Class Glutathione-S-Transferase in Fetal and Pediatric Filum Terminale Samples: A Comparative Study

Medicina ◽  
2019 ◽  
Vol 55 (5) ◽  
pp. 133
Author(s):  
Cahit Kural ◽  
Serpil Oguztuzun ◽  
Gülçin Güler Şimşek ◽  
Servet Guresci ◽  
Pınar Kaygın ◽  
...  
Keyword(s):  
Tethered Cord ◽  
Filum Terminale ◽  
Glutathione S Transferase ◽  
Chi Square ◽  
Significant Difference ◽  
Chi Square Test ◽  
Normal Human ◽  
Group 2 ◽  
The Relationship ◽  
Group 1

Background and objectives: The pathophysiology of tethered cord syndrome (TCS) in children is not well elucidated. An inelastic filum terminale (FT) is the main factor underlying the stretching of the spinal cord in TCS. Our study aimed to investigate the expression of glutathione-S-transferase (GST) in children and fetal FT samples in order to understand the relationship between this enzyme expression and the development of TCS. Materials and Methods: FT samples were obtained from ten children with TCS (Group 1) and histological and immunohistochemical examinations were performed. For comparison, FT samples from fifteen normal human fetuses (Group 2) were also analyzed using the same techniques. Statistical comparison was made using a Chi-square test. Results: Positive GST-sigma expression was detected in eight (80%) of 10 samples in Group 1. The positive GST-sigma expression was less frequent in nine (60%) of 15 samples from Group 2. No statistically significant difference was detected between the two groups (p = 0.197). Conclusions: Decreased FT elasticity in TCS may be associated with increased GST expression in FT. More prospective studies are needed to clarify the mechanism of the GST–TCS relationship in children.

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