scholarly journals New Species of Soldier Fly—Sargus bipunctatus (Scopoli, 1763) (Diptera: Stratiomyidae), Recorded from a Human Corpse in Europe—A Case Report

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 302
Author(s):  
Marek Michalski ◽  
Piotr Gadawski ◽  
Joanna Klemm ◽  
Krzysztof Szpila
Keyword(s):  
Case Report ◽  
New Species ◽  
Dna Barcoding ◽  
Forensic Entomology ◽  
Morphological Characters ◽  
Lower Limbs ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
Practical Applications ◽  
Human Corpse

The only European Stratiomyidae species known for feeding on human corpses was the black soldier fly Hermetia illucens (Linnaeus, 1758). Analysis of fauna found on a human corpse, discovered in central Poland, revealed the presence of feeding larvae of another species from this family: the twin-spot centurion fly Sargus bipunctatus (Scopoli, 1763). The investigated corpse was in a stage of advanced decomposition. The larvae were mainly observed in the adipocere formed on the back and lower limbs of the corpse, and in the mixture of litter and lumps of adipocere located under the corpse. Adult specimens and larvae were identified based on morphological characters, and final identification was confirmed using DNA barcoding. Implementing a combination of morphological and molecular methods provided a reliable way for distinguishing the larvae of S. bipunctatus and H. illucens. The potential of S. bipunctatus for practical applications in forensic entomology is currently difficult to assess. Wide and reliable use of S. bipunctatus in the practice of forensic entomology requires further studies of the bionomy of this fly.

Dichrorampha dinarica, new species, a century of confusion in European lepidopterology (Lepidoptera: Tortricidae) resolved by combining morphology and DNA barcoding

Zootaxa ◽  
2012 ◽  
Vol 3389 (1) ◽  
pp. 41 ◽  
Author(s):  
PETER HUEMER ◽  
BOYAN ZLATKOV ◽  
JOAQUIN BAIXERAS
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Bosnia And Herzegovina ◽  
Morphological Characters ◽  
Dinaric Mountains ◽  
Significant Divergence ◽  
The Alps

Dichrorampha dinarica, new species, is described from the Dinaric mountains (Macedonia, Bosnia and Herzegovina). The newspecies was formerly confused with D. ligulana (Herrich-Schäffer, 1851) from the Alps and D. rilana Drenowsky, 1909 fromthe Rila mountains (Bulgaria). Therefore, a re-description of D. rilana is provided based on topotypical specimens. The diag-nostic morphological characters of the involved species, supported by significant divergence of the mtDNA barcode (COI fragment of 658 bp), are discussed in detail.

Revision of the genus Prometopidia Hampson, 1902, with description of the new species P. joshimathensis sp. nov. from West-Himalaya and its subspecies P. j. yazakii ssp. nov. from Nepal (Lepidoptera: Geometridae, Ennominae)

Zootaxa ◽  
2021 ◽  
Vol 4980 (1) ◽  
pp. 28-44
Author(s):  
PRITHA DEY ◽  
VIRENDRA PRASAD UNIYAL ◽  
AXEL HAUSMANN ◽  
DIETER STÜNING
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Type Species ◽  
Morphological Characters ◽  
Genetic Differences ◽  
New Taxon ◽  
Correct Position ◽  
Punjab Province ◽  
West Himalaya ◽  
Female Holotype

The genus Prometopidia Hampson, 1902 and its type-species P. conisaria Hampson, 1902 are redescribed and newly discovered morphological characters are explained. The female holotype of Prometopidia arenosa Wiltshire, 1961, was studied and the species redescribed, its correct position in Prometopidia is verified. The new species P. joshimathensis sp. nov. is described from Joshimath area in India, Uttarakhand province. Sympatric with P. conisaria at Joshimath, P. joshimathensis also occurs at Shimla, Punjab province, and in central and eastern Nepal. Morphological and genetic differences found in the specimens of Nepal are considered subspecific, justifying the new taxon P. joshimathensis yazakii ssp. nov. Types and specimens of Prometopidia across its whole range of distribution from Afghanistan to Nepal, habitats, genitalia, remarkable morphological characters and DNA barcoding-results are figured. 

Evaluation of Development Datasets for Hermetia illucens (L.) (Diptera: Stratiomyidae) for Estimating the Time of Placement of Human and Swine Remains in Texas, USA

2021 ◽  
Author(s):  
Lue Cuttiford ◽  
Meaghan L Pimsler ◽  
Chong Chin Heo ◽  
Le Zheng ◽  
Inoka Karunaratne ◽  
...  
Keyword(s):  
Forensic Entomology ◽  
Time Of Death ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
Basic Tenet ◽  
Accuracy And Precision ◽  
Basic Understanding ◽  
San Marcos ◽  
Development Data ◽  
Improved Accuracy

Abstract A basic tenet of forensic entomology is development data of an insect can be used to predict the time of colonization (TOC) by insect specimens collected from remains, and this prediction is related to the time of death and/or time of placement (TOP). However, few datasets have been evaluated to determine their accuracy or precision. The black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae) is recognized as an insect of forensic importance. This study examined the accuracy and precision of several development datasets for the black soldier fly by estimating the TOP of five sets of human and three sets of swine remains in San Marcos and College Station, TX, respectively. Data generated from this study indicate only one of these datasets consistently (time-to-prepupae 52%; time-to-eclosion 75%) produced TOP estimations that occurred within a day of the actual TOP of the remains. It is unknown if the precolonization interval (PreCI) of this species is long, but it has been observed that the species can colonize within 6 d after death. This assumption remains untested by validation studies. Accounting for this PreCI improved accuracy for the time-to-prepupae group, but reduced accuracy in the time-to-eclosion group. The findings presented here highlight a need for detailed, forensic-based development data for the black soldier fly that can reliably and accurately be used in casework. Finally, this study outlines the need for a basic understanding of the timing of resource utilization (i.e., duration of the PreCI) for forensically relevant taxa so that reasonable corrections may be made to TOC as related to minimum postmortem interval (mPMI) estimates.

Effectiveness of DNA barcoding markers in the description of a new and unusual calyptrate species of Myrcianthes (Myrtaceae)

Phytotaxa ◽  
2016 ◽  
Vol 284 (3) ◽  
pp. 203
Author(s):  
CARLOS PARRA-O. ◽  
A. F. BOHÓRQUEZ-OSORIO
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Morphological Characters ◽  
Unusual Combination ◽  
A New Species

A new species, Myrcianthes roncesvallensis is described and illustrated from Andean forests of Tolima, Colombia. Due to the unusual combination of morphological characters in this new species never been found previously in a Colombian Myrtaceae, such as having a closed calyx, dichasial inflorescence and an eugenioid embryo, three DNA barcoding markers (rbcL, matK and ITS) were used to confirm the genus in which this species should be described. Taxonomic affinities of the new species within the genus are discussed.

scholarly journals Description of a new species of Loxosceles Heineken & Lowe (Araneae, Sicariidae) recluse spiders from Hidalgo, Mexico, under integrative taxonomy: morphological and DNA barcoding data (CO1 + ITS2)

2020 ◽  
Author(s):  
Claudia Isabel Navarro-Rodríguez ◽  
Alejandro Valdez-Mondragón
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Species Delimitation ◽  
Sister Group ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Taxonomic Approach ◽  
Morphological Differences ◽  
Unique Species ◽  
A New Species

Based on an integrative taxonomic approach, a new species of the genus Loxosceles Heineken & Lowe, 1832, is described from the state of Hidalgo, Mexico. Loxosceles tolantongo sp. nov. is described based on DNA barcoding using cytochrome c oxidase subunit 1 (CO1) and internal transcribed spacer 2 (ITS2), and morphology. For species delimitation, four molecular methods were implemented: 1) corrected p-distances under neighbor joining (NJ); 2) automatic barcode gap discovery (ABGD); 3) general mixed yule coalescent model (GMYC) and 4) Bayesian Poisson tree processes (bPTP). The new species morphologically resembles L. jaca, another species from Hidalgo, but there are morphological differences mainly in the tibiae of the male palp, the seminal receptacles of the females and also the high genetic p-distances. CO1 was more informative than ITS2 for the genetic separation; however, both concatenated genes (CO1 + ITS2) present robust evidence for species delimitation. Loxosceles tolantongo sp. nov. is considered a unique species for four reasons: 1) it can be diagnosed and distinguished by morphological characters (of the male palps mainly, but also of the seminal receptacles of the females); 2) the genetic p-distances with CO1 were high (>10%); 3) the molecular species delimitation methods were congruent under CO1 and CO1 + ITS2; and 4) under CO1 and CO1 + ITS2, the new species is a putative sister group of L. jaca + L. tenango.

Description of a new species of the genus Maua Distant (Hemiptera, Cicadidae) from China

Zootaxa ◽  
2021 ◽  
Vol 4949 (3) ◽  
pp. 582-588
Author(s):  
JIALI WANG ◽  
JOANNES P. DUFFELS ◽  
CONG WEI
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Intraspecific Variation ◽  
Male Genitalia ◽  
Morphological Characters ◽  
Coi Gene ◽  
Morphological Observation ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
A New Species

A new species, Maua squeala sp. nov., is described from China. This species is similar to M. affinis Distant, 1905 and M. palawanensis Duffels, 2009, but can be distinguished by the shorter and more slender body of the new species, the lateral fasciae on the mesonotum and the shape of the male genitalia. The intraspecific variation of this species is discussed based on morphological observation combined with sequences of partial mitochondrial COI gene (DNA barcoding) of individuals exhibiting different morphological characters. 

Pinellia hunanensis (Araceae), a new species supported by morphometric analysis and DNA barcoding

Phytotaxa ◽  
2013 ◽  
Vol 130 (1) ◽  
pp. 1 ◽  
Author(s):  
Steven G Newmaster ◽  
YU-JING LIU ◽  
XIAN-JIN WU ◽  
YUE LIU ◽  
Subramanyam Ragupathy ◽  
...  
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Function Analysis ◽  
Detrended Correspondence Analysis ◽  
Morphological Characters ◽  
Preliminary Evidence ◽  
Morphometric Variation ◽  
Further Development ◽  
A New Species

Pinellia hunanensis, a new species from China, is described and illustrated. A key for the identification of all Pinellia species in China, Korea and Japan is included. A detrended correspondence analysis identified 6 groups of taxa including the new species. From the 20 samples, analyzing 38 morphological characters. A discriminant function analysis was used to rigorously test the classification of specimens provided in the cluster analysis. DNA barcoding provided phylogenetic support using NJ and Bayesian methods to distinguish all six taxa including the putative new species. This study provides preliminary evidence of morphometric variation within and among species of Pinellia, which allows further development of hypothesis concerning species boundaries. Discussions concerning medicinal product substitution within the genus Pinellia are presented in the context of conservation initiatives of species in China.

Integrative taxonomy of Mexican cycads: biogeography, morphology and DNA barcoding corroborate a new sympatric species in Ceratozamia (Zamiaceae)

Phytotaxa ◽  
2016 ◽  
Vol 268 (1) ◽  
pp. 25 ◽  
Author(s):  
LILÍ MARTÍNEZ-DOMÍNGUEZ ◽  
FERNANDO NICOLALDE-MOREJÓN ◽  
FRANCISCO VERGARA-SILVA ◽  
DENNIS W. STEVENSON
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Taxonomic Status ◽  
Morphological Characters ◽  
Sympatric Species ◽  
Integrative Taxonomy ◽  
Los Tuxtlas ◽  
A New Species

A new species, from the montane region of Los Tuxtlas (state of Veracruz, Mexico), Ceratozamia subroseophylla, is proposed in accordance with the integrative taxonomy framework and through application of the taxonomic circle rationale. Our identification of this new species is the result of a thorough re-evaluation of four formerly described Ceratozamia species, namely: (1) C. miqueliana, with which it overlaps geographically; (2) C. mexicana; (3) C. robusta, which has also been previously seen as morphologically similar to C. miqueliana; and (4) C. brevifrons, which was at the center of a controversy concerning the morphological and geographic limits when compared to C. mexicana. Even though there are morphometric affinities of C. subroseophylla with this set of species, there are apomorphies in qualitative morphological characters and diagnostic nucleotidic variants established through character-based DNA barcoding to allow the recognition of this new species. The implications that our integrative taxonomy-based assessment of systematic diversity in Ceratozamia species from Veracruz have for evaluating the overall taxonomic status of the genus are discussed.

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