scholarly journals The Effect of Polydeoxyribonucleotide Extracted from Salmon Sperm on the Restoration of Bisphosphonate-Related Osteonecrosis of the Jaw

Marine Drugs ◽  
2019 ◽  
Vol 17 (1) ◽  
pp. 51 ◽  
Author(s):  
Deok-Won Lee ◽  
Hoon Hyun ◽  
Sungsu Lee ◽  
So Kim ◽  
Gyu-Tae Kim ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Rat Model ◽  
Osteonecrosis Of The Jaw ◽  
Local Administration ◽  
High Concentration ◽  
Necrotic Bone ◽  
Specific Concentration

Bisphosphonates (BPs) used for treating skeletal diseases can induce bisphosphonate-related osteonecrosis of the jaw (BRONJ). Despite much effort, effective remedies are yet to be established. In the present study, we investigated the feasibility of polydeoxyribonucleotide (PDRN) extracted from salmon sperm for the treatment of BRONJ, in a BRONJ-induced rat model. Compared with BRONJ-induced samples, PDRN-treated samples exhibited lower necrotic bone percentages and increased numbers of blood vessels and attached osteoclast production. Moreover, local administration of PDRN at a high concentration (8 mg/kg) remarkably resolved the osteonecrosis. Findings from this study suggest that local administration of PDRN at a specific concentration may be considered clinically for the management of BRONJ.

Simultaneous measurement of two major prostacyclin metabolites in urine

1990 ◽  
Vol 36 (11) ◽  
pp. 1978-1980 ◽  
Author(s):  
S Zureick ◽  
J Nadler ◽  
J Yamamoto ◽  
R Horton
Keyword(s):  
Angiotensin Ii ◽  
Blood Vessels ◽  
Simultaneous Measurement ◽  
Healthy Subjects ◽  
High Accuracy ◽  
High Concentration ◽  
Accuracy And Precision ◽  
Overall Evaluation ◽  
Excretion Rates ◽  
Apparently Healthy

Abstract We describe a combined HPLC-RIA technique to measure both major metabolites of prostacyclin (PGI2): 6-keto PGF1 alpha and 2,3-dinor-6 keto PGF1 alpha. The measurement of the former, which originates from renal blood vessels, and the latter, from systemic vessels and the liver, may provide a better overall evaluation of production than measurement of one metabolite. An aliquot of acidified urine with added 3H-labeled metabolites is adsorbed and then eluted from a C18 Bond-Elut column. The sample is then passed through an HPLC system by use of an isocratic solvent combination that separates the two metabolites from known prostaglandins. The purified metabolites are then quantified by RIA. Using a logit-log10 transform, one can measure between 12 and 250 pg of either metabolite, with high accuracy and precision (CVs of 12% for a low concentration and 7% for a high concentration). Reference values for apparently healthy subjects were, respectively, 107 (SD 45) and 171 (SD 69) ng/g creatinine for 6-keto PGF1 alpha and the dinor metabolite in men (n = 18) and 45 (SD 22) and 141 (SD 28) ng/g creatinine, respectively, in women (n = 15). Indomethacin in standard doses reduced both metabolite values by 50%. Intravenous administration of angiotensin II (5 ng/kg of body wt per minute) did not alter excretion rates, but equipressor doses of norepinephrine (0.1 microgram/kg per minute) increased the production of both metabolites (6-keto greater than dinor).

Retinal blood vessels are damaged in a rat model of NMDA-induced retinal degeneration

2010 ◽  
Vol 485 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Kaori Ueda ◽  
Tsutomu Nakahara ◽  
Maya Hoshino ◽  
Asami Mori ◽  
Kenji Sakamoto ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Retinal Degeneration ◽  
Rat Model ◽  
Retinal Blood Vessels

Effects of insulin-like growth factor–I and platelet-rich plasma on sciatic nerve crush injury in a rat model

2011 ◽  
Vol 114 (2) ◽  
pp. 522-528 ◽  
Author(s):  
Erhan Emel ◽  
Selma Sönmez Ergün ◽  
Dilcan Kotan ◽  
Esra Başar Gürsoy ◽  
Yeşim Parman ◽  
...  
Keyword(s):  
Functional Recovery ◽  
Rat Model ◽  
Platelet Rich Plasma ◽  
Sensory Function ◽  
Local Administration ◽  
Control Group ◽  
Factor I ◽  
Igf I ◽  
Group 2 ◽  
Group 1

Object Local administration of insulin-like growth factor–I (IGF-I) has been shown to increase the rate of axon regeneration in crush-injured and freeze-injured rat sciatic nerves. Local administration of platelet-rich plasma (PRP) has been also shown to have a measurable effect on facial nerve regeneration after transection in a rat model. The objective of the study was to compare the effects of locally administered IGF-I and PRP on the parameters of the Sciatic Function Index (SFI), sensory function (SF), axon count, and myelin thickness/axon diameter ratio (G-ratio) in a rat model of crush-injured sciatic nerves. Methods The right sciatic nerve of Wistar albino rats (24 animals) was crushed using a Yasargil-Phynox aneurysm clip for 45 minutes. All animals were randomly divided into 3 groups: Group 1 (control group) was treated with saline, Group 2 was treated with IGF-I, and Group 3 was treated with PRP. Injections were performed using the tissue expander's injection port with a connecting tube directed at the crush-injured site. Functional recovery was assessed with improvement in the SFI. Recovery of sensory function was using the pinch test. Histopathological examination was performed 3 months after the injury. Results The SFI showed an improved functional recovery in the IGF-I–treated animals (Group 2) compared with the saline-treated animals (Group 1) 30 days after the injury. In IGF-I–treated rats, sensory function returned to the baseline level significantly faster than in saline-treated and PRP-treated rats as shown in values between SF-2 and SF-7. The G-ratios were found to be significantly higher in both experimental groups than in the control group. Conclusions This study suggests that the application of IGF-I to the crush-injured site may expedite the functional recovery of paralyzed muscle by increasing the rate of axon regeneration.

Local Administration of Thiamine Ameliorates Ongoing Pain in a Rat Model of Second-Degree Burn

2017 ◽  
Vol 38 (5) ◽  
pp. e842-e850 ◽  
Author(s):  
Kangkang Zhang ◽  
Yali Pei ◽  
Zhihao Gan ◽  
Xuetao Zhang ◽  
Ye Duan ◽  
...  
Keyword(s):  
Rat Model ◽  
Local Administration ◽  
Degree Burn ◽  
Ongoing Pain ◽  
Second Degree

scholarly journals Local administration of stem cell-derived extracellular vesicles in a thermoresponsive hydrogel promotes a pro-healing effect in a rat model of colo-cutaneous post-surgical fistula

Nanoscale ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 218-232
Author(s):  
Arthur Berger ◽  
Irami Araújo-Filho ◽  
Max Piffoux ◽  
Alba Nicolás-Boluda ◽  
Alice Grangier ◽  
...  
Keyword(s):  
Stem Cell ◽  
Minimally Invasive ◽  
Therapeutic Effect ◽  
Extracellular Vesicles ◽  
Residence Time ◽  
Rat Model ◽  
Local Administration ◽  
Thermoresponsive Hydrogel ◽  
Healing Effect

Local minimally-invasive EV delivery on a thermo-actuated PF-127 gel enhanced EV residence time in colo-cutaneous fistulas promoting a therapeutic effect.

scholarly journals Mumefural Improves Blood Flow in a Rat Model of FeCl3-Induced Arterial Thrombosis

Nutrients ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3795
Author(s):  
Jihye Bang ◽  
Won Kyung Jeon
Keyword(s):  
Blood Flow ◽  
Blood Vessels ◽  
Rat Model ◽  
Arterial Thrombosis ◽  
Thrombus Formation ◽  
Sprague Dawley ◽  
Fiber Damage ◽  
Sprague Dawley Rats ◽  
Related Proteins

Mumefural (MF), a bioactive component of the processed fruit of Prunus mume Sieb. et Zucc, is known to inhibit platelet aggregation induced by agonists in vitro. In this study, we investigated the anti-thrombotic effects of MF using a rat model of FeCl3-induced arterial thrombosis. Sprague–Dawley rats were intraperitoneally injected with MF (0.1, 1, or 10 mg/kg) 30 min before 35% FeCl3 treatment to measure the time to occlusion using a laser Doppler flowmeter and to assess the weight of the blood vessels containing thrombus. MF treatment significantly improved blood flow by inhibiting occlusion and thrombus formation. MF also prevented collagen fiber damage in injured vessels and inhibited the expression of the platelet activation-related proteins P-selectin and E-selectin. Moreover, MF significantly reduced the increased inflammatory signal of nuclear factor (NF)-κB, toll-like receptor 4 (TLR4), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in blood vessels. After administration, MF was detected in the plasma samples of rats with a bioavailability of 36.95%. Therefore, we suggest that MF may improve blood flow as a candidate component in dietary supplements for improving blood flow and preventing blood circulation disorders.

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