scholarly journals PHA Production from Cheese Whey and “Scotta”: Comparison between a Consortium and a Pure Culture of Leuconostoc mesenteroides

2021 ◽  
Vol 9 (12) ◽  
pp. 2426
Author(s):  
Francesca Bosco ◽  
Simona Cirrincione ◽  
Riccardo Carletto ◽  
Luca Marmo ◽  
Francesco Chiesa ◽  
...  
Keyword(s):  
Cheese Whey ◽  
Ph Control ◽  
Microbial Culture ◽  
Bacterial Strains ◽  
Mixed Microbial Culture ◽  
Aquatic Life ◽  
Pure Cultures ◽  
Industrial Byproducts ◽  
Pha Production ◽  
Synthetic Media

It is urgent to expand the market of biodegradable alternatives to oil-derived plastics owing to (i) increasingly limited oil availability/accessibility, and (ii) the dramatic impact of traditional plastics on aquatic life, the food chain, all Earth ecosystems, and ultimately, human health. Polyhydroxyalkanoates (PHAs) are promising biodegradable polymers that can be obtained through microbial fermentation of agro-industrial byproducts, e.g., milk and cheese whey. Here, the PHA-accumulating efficiency of a mixed microbial culture (MMC, derived from activated sludges) grown on dairy byproducts (cheese and scotta whey) was measured. Bioreactor tests featuring temperature and pH control showed that both scotta and pre-treated Toma cheese whey could be used for PHA production by MMC, although scotta cheese whey supported higher PHA yield and productivity. The advantages of open MMCs include their plasticity and versatility to fast changing conditions; furthermore, no growth-medium sterilization is needed prior to fermentation. However, the use of pure cultures of efficient PHA producers may support better metabolic performances. Therefore, PHA-producing strains were isolated from an MMC, leading to the satisfactory identification of two bacterial strains, Citrobacter freundii and Leuconostoc spp., whose ability to accumulate PHAs in synthetic media was confirmed. A more detailed investigation by mass spectrometry revealed that the strain was L. mesenteroides. Although the validation of L. mesenteroides’ potential to produce PHA through fermentation of agro-industrial byproducts requires further investigations, this is the first study reporting PHA production with the Leuconostoc genus.

scholarly journals Enhanced polyhydroxyalkanoate (PHA) production from the organic fraction of municipal solid waste by using mixed microbial culture

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Bianca Colombo ◽  
Francesca Favini ◽  
Barbara Scaglia ◽  
Tommy Pepè Sciarria ◽  
Giuliana D’Imporzano ◽  
...  
Keyword(s):  
Municipal Solid Waste ◽  
Solid Waste ◽  
Microbial Culture ◽  
Organic Fraction ◽  
Mixed Microbial Culture ◽  
Pha Production

The relationship between mixed microbial culture composition and PHA production performance from fermented molasses

2014 ◽  
Vol 31 (4) ◽  
pp. 257-263 ◽  
Author(s):  
Gilda Carvalho ◽  
Adrian Oehmen ◽  
Maria G.E. Albuquerque ◽  
Maria A.M. Reis
Keyword(s):  
Production Performance ◽  
Microbial Culture ◽  
Mixed Microbial Culture ◽  
Pha Production ◽  
The Relationship

Polyhydroxyalkanoates (PHAs) production from fermented cheese whey by using a mixed microbial culture

2016 ◽  
Vol 218 ◽  
pp. 692-699 ◽  
Author(s):  
Bianca Colombo ◽  
Tommy Pepè Sciarria ◽  
Maria Reis ◽  
Barbara Scaglia ◽  
Fabrizio Adani
Keyword(s):  
Cheese Whey ◽  
Microbial Culture ◽  
Mixed Microbial Culture

Characterization of Nostoc muscorum NCCU-442 Derived Poly-3- hydroxybutyrate (PHB) and Polyethylene Glycol (PEG) Blends

2020 ◽  
Vol 10 (3) ◽  
pp. 200-207
Author(s):  
Sabbir Ansari ◽  
Tasneem Fatma
Keyword(s):  
Thermal Properties ◽  
Polyethylene Glycol ◽  
Morphological Properties ◽  
Morphological Alteration ◽  
Nostoc Muscorum ◽  
Microbial Culture ◽  
Polymer Science ◽  
Mixed Microbial Culture ◽  
Scanning Calorimetry ◽  
Casting Technique

Background: Poly-3-hydroxybutyrate (PHB) has attracted much consideration as biodegradable biocompatible polymer. This thermoplastic polymer has comparable material properties to polypropylene. Materials with more valuable properties may result from blending, a common practice in polymer science. Objective: In this paper, blends of PHB (extracted from cyanobacterium Nostoc muscorum NCCU- 442 with polyethylene glycol (PEG) were investigated for their thermal, tensile, hydrophilic and biodegradation properties. Methods: Blends were prepared in different proportions of PHB/PEG viz. 100/0, 98/2, 95/5, 90/10, 80/20, and 70/30 (wt %) using solvent casting technique. Morphological properties were investigated by using Scanning Electron Microscopy (SEM). Differential scanning calorimetry and thermogravimetric analysis were done for thermal properties determination whereas the mechanical and hydrophilic properties of the blends were studied by means of an automated material testing system and contact angle analyser respectively. Biodegradability potential of the blended films was tested as percent weight loss by mixed microbial culture within 60 days. Results: The blends showed good misciblity between PEG and PHB, however increasing concentrations of plasticizer caused morphological alteration as evidenced by SEM micrographs. PEG addition (10 % and above) showed significant alternations in the thermal properties of the blends. Increase in the PEG content increased the elongation at break ratio i.e enhanced the required plasticity of PHB. Rate of microbial facilitated degradation of the blends was greater with increasing PEG concentrations. Conclusion: Blending with PEG increased the crucial polymeric properties of cyanobacterial PHB.

Kinetics of growth and multi substrate degradation by an indigenous mixed microbial culture isolated from a wastewater treatment plant in Guwahati, India

2008 ◽  
Vol 58 (5) ◽  
pp. 1101-1106
Author(s):  
Pichiah Saravanan ◽  
K. Pakshirajan ◽  
P. K. Saha
Keyword(s):  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Lag Phase ◽  
Microbial Culture ◽  
High Concentration ◽  
Mixed Microbial Culture ◽  
Phenol Biodegradation ◽  
Low Concentration ◽  
Culture Growth

An indigenous mixed culture of microorganisms, isolated from a sewage treatment plant, was investigated for its potential to simultaneously degrade phenol and m-cresol during its growth in batch shake flasks. 22 full factorial designs with the two substrates as the factors, at two different levels and two different initial concentration ranges, were employed to carry out the biodegradation experiments. For complete utilisation of phenol and m-cresol, the culture took a minimum duration of 21 hrs at their low concentration of 100 mg/L each, and a maximum duration of 187 hrs at high concentration of 600 mg/L each in the multisubstrate system. The biodegradation results also showed that the presence of phenol in low concentration range (100–300 mg/L did not inhibit m-cresol biodegradation; on the other hand, presence of m-cresol inhibited phenol biodegradation by the culture. Moreover, irrespective of the concentrations used, phenol was degraded preferentially and earlier than m-cresol. During the culture growth, a lag phase was observed above a combined concentration of 500 mg/L i.e., 200 mg/L m-cresol and 300 mg/L of phenol and above). Statistical analysis of the specific growth rate of the culture in the multisubstrate system was also performed in the form of ANOVA and Student ‘t’ test, which gave good interpretation in terms of main and interaction effects of the substrates.

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