scholarly journals Anthocyanin-Rich Grape Pomace Extract (Vitis vinifera L.) from Wine Industry Affects Mitochondrial Bioenergetics and Glucose Metabolism in Human Hepatocarcinoma HepG2 Cells

Molecules ◽  
2018 ◽  
Vol 23 (3) ◽  
pp. 611 ◽  
Author(s):  
Nathalia de Sales ◽  
Leandro Silva da Costa ◽  
Talita Carneiro ◽  
Daniela Minuzzo ◽  
Felipe Oliveira ◽  
...  
2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986290 ◽  
Author(s):  
Massimo Tacchini ◽  
Ilaria Burlini ◽  
Immacolata Maresca ◽  
Alessandro Grandini ◽  
Tatiana Bernardi ◽  
...  

Vitis vinifera L. leaves from pruning are by-products of the wine industry and represent an important source of secondary raw material, thanks to their polyphenols content. Optimization of the extraction processes is a key factor for their valorization, and Design of Experiment (DOE) could be a tool to obtain the most performing extract in terms of polyphenols quality/quantity and bioactivity. Vitis vinifera Lambrusco leaves were subjected to ultrasound-assisted extractions guided by a 23 factorial design. Three independent parameters (% solvent, time of extraction, and solvent:solid ratio) were considered to evaluate the extraction process by analyzing the extraction yield, the total phenolic content (Folin-Ciocalteu assay), and the antioxidant capacity (DPPH assay). Moreover, the content of the main molecules was identified and quantified by reversed-phase high-performance liquid chromatography coupled with diode array detection and mass spectrometry. The DOE highlighted the best extraction conditions that showed slight changes considering the different evaluating parameters. The highest extraction yield was obtained by extraction with 100% water, 60 minutes of extraction time, and 30:1 solvent:solid ratio, but it was neither the richest in polyphenols nor antioxidant capacity. The latter 2 characteristics were associated with the extraction performed using 50% ethanol, 35 minutes of extraction time, and a 20:1 solvent:solid ratio. That extract also exhibited the highest quantity of flavonols.


2019 ◽  
Vol 10 (4) ◽  
pp. 1856-1869 ◽  
Author(s):  
Joana R. Costa ◽  
Manuela Amorim ◽  
Ana Vilas-Boas ◽  
Renata V. Tonon ◽  
Lourdes M. C. Cabral ◽  
...  

Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including in gastrointestinal health.


Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1176
Author(s):  
Jana Žiarovská ◽  
Lucia Zamiešková ◽  
Jana Bilčíková ◽  
Veronika Fialková ◽  
Jozef Sabo ◽  
...  

The aim of this study was to compare the natural variability of the expression of class I chitinase gene in grapes of Vitis vinifera L. varieties as well as investigate their antimicrobial activity. Further, the effect of antimicrobial activity in chitinase expression was analyzed. Matured berries of thirteen grape varieties were used in the study—Alibernet, Dornfelder, Cabernet Sauvignon, Blaufränkisch, Sauvignon Blanc, Welschriesling, Weisser Riesling, Irsai Oliver, Pinot Blanc, Pálava, Müller-Thurgau, Grűner Veltliner, and Feteasca Regala, grown in the Small Carpathians wine region of Slovakia, Vrbové. Chitinase I gene expression was found to be similar in most of the analyzed varieties with the except of two varieties, where the expression of chitinase I was significantly higher—Pinot Blanc and Savignon Blanc. By comparing the varieties between the lowest and highest expression fold change, they were statistically different in the level of its chitinase I gene expression. Grape pomace extracts of analyzed Vitis vinifera L. were effective against Gram-positive, Gram-negative bacteria and yeasts, but a weak correlation tendency was found between chitinase I gene expression and antimicrobial activity, only against Staphylococcus aureus subsp. aureus, Candida albicans, and Candida glabrata.


Meat Science ◽  
2019 ◽  
Vol 147 ◽  
pp. 6-12 ◽  
Author(s):  
Obert C. Chikwanha ◽  
Voster Muchenje ◽  
Joubert E. Nolte ◽  
Michael E.R. Dugan ◽  
Cletos Mapiye

2005 ◽  
Vol 220 (3-4) ◽  
pp. 431-437 ◽  
Author(s):  
Dietmar Kammerer ◽  
Jasenka Gajdos Kljusuric ◽  
Reinhold Carle ◽  
Andreas Schieber

2014 ◽  
Vol 62 (43) ◽  
pp. 10552-10560 ◽  
Author(s):  
Y. Arrieta-Garay ◽  
P. Blanco ◽  
C. López-Vázquez ◽  
J. J. Rodríguez-Bencomo ◽  
J. R. Pérez-Correa ◽  
...  

Pharmaceutics ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1148
Author(s):  
Alexandra A. Hübner ◽  
Fernanda D. Sarruf ◽  
Camila A. Oliveira ◽  
Alberto V. Neto ◽  
Dominique C. H. Fischer ◽  
...  

In winemaking, a large amount of grape pomace is produced that is rich in polyphenolics and highly beneficial for human health, as phenols are useful for skin ultraviolet (UV) protection. In this investigation, we evaluated the safety and clinical efficacy of a sunscreen system containing a grape pomace extract from Vitis vinifera L. as a bioactive ingredient. The recovery of phenolics in the waste was performed by percolation. Nine emulsions were developed using a factorial design and two were evaluated clinically: Formulation E, containing only UV filters (butylmethoxydibenzoyl methane, ethylhexyl methoxycinnamate and ethylhexyl dimethyl PABA), and F, with the extract at 10.0% w/w + UV filters. The antioxidant activity was determined by the DPPH assay and the in vitro efficacy was established by sun protection factor (SPF) measurements (Labsphere UV-2000S). Clinical tests were performed to determine safety (human repeated insult patch test) and to confirm efficacy (photoprotective effectiveness in participants). The results showed a synergistic effect between the sunscreen system and the extract on UVB protection and antioxidant activity. Both samples were considered safe. Formulation F was 20.59% more efficient in protecting skin against UVB radiation, taking approximately 21% more time to induce erythema compared to the extract-free sample.


2014 ◽  
Vol 34 (1) ◽  
pp. 135-142 ◽  
Author(s):  
Eldina Castro Sousa ◽  
Ana Maria Athayde Uchôa-Thomaz ◽  
José Osvaldo Beserra Carioca ◽  
Selene Maia de Morais ◽  
Alessandro de Lima ◽  
...  

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