scholarly journals Analysis of Processing Effects on Glucosinolate Profiles in Red Cabbage by LC-MS/MS in Multiple Reaction Monitoring Mode

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5171
Author(s):  
Weicheng Wu ◽  
Jingqiu Chen ◽  
Dandan Yu ◽  
Shiguo Chen ◽  
Xingqian Ye ◽  
...  
Keyword(s):  
Multiple Reaction Monitoring ◽  
Glucosinolate Content ◽  
Reaction Monitoring ◽  
Red Cabbage ◽  
Indole Glucosinolates ◽  
Aliphatic Glucosinolates ◽  
Multiple Reaction Monitoring Mode ◽  
Health Promoting ◽  
Processing Effects ◽  
To Receive

Red cabbage (Brassica oleracea L. var. capitata) continues to receive increasing attention on its health-promoting properties because of its high glucosinolate content. Glucosinolates are an unstable active substance; however, there are few studies on their changes in different cooking processes. In this study, we investigated the effects of processing methods (boiling, steaming, microwave heating, frying, stir-frying) and boiling time on glucosinolates in red cabbage. Ten glucosinolates, including 4-methoxyglucobrassicin, neoglucobrassicin, glucoalyssin, glucobrassicin, glucoraphanin, glucoiberin, progoitrin, gluconapin and sinigrin, in red cabbage were detected. Decreases of 32.36%, 24.83%, 25.27%, 81.11% and 84.29% for total glucosinolates were observed after boiling, microwaving, steaming, frying and stir-frying. Indole glucosinolates were more efficiently lost compared to aliphatic glucosinolates after boiling, while microwaving, steaming, frying and stir-frying also resulted in a greater reduction in indole glucosinolates than aliphatic glucosinolates. Glucoalyssin, glucoerucin and sinigrin were more thermal sensitive than other glucosinolates. It was confirmed that microwaving and steaming retained higher levels of glucosinolates than other methods and may be better for cooking red cabbage.

scholarly journals Tyrosol and Hydroxytyrosol Determination in Extra Virgin Olive Oil with Direct Liquid Electron Ionization–Tandem Mass Spectrometry

Separations ◽  
2021 ◽  
Vol 8 (10) ◽  
pp. 173
Author(s):  
Veronica Termopoli ◽  
Maurizio Piergiovanni ◽  
Achille Cappiello ◽  
Pierangela Palma ◽  
Giorgio Famiglini
Keyword(s):  
Olive Oil ◽  
Matrix Effects ◽  
Multiple Reaction Monitoring ◽  
Virgin Olive Oil ◽  
Standard Addition ◽  
Extra Virgin Olive Oil ◽  
Electron Ionization ◽  
Free Form ◽  
Multiple Reaction Monitoring Mode ◽  
Health Promoting

Extra virgin olive oil (EVOO) is one of the main ingredients of the Mediterranean diet. It is claimed as a functional food for its unique content of health-promoting compounds. Tyrosol (Tyr), Hydroxytyrosol (Htyr), and their phenolic derivatives present in EVOO show beneficial properties, and their identification and quantification, both in their free form and after the hydrolysis of more complex precursors, are important to certify its quality. An alternative method for quantifying free and total Tyr and Htyr in EVOO is presented using an LC–MS interface based on electron ionization (EI), called liquid electron ionization (LEI). This method requires neither sample preparation nor chromatography; the sample is diluted and injected. The selectivity and sensitivity were assessed in multiple reaction monitoring mode (MRM), obtaining confirmation and quantification in actual samples ranging from 5 to 11 mg/Kg for the free forms and from 32 to 80 mg/Kg for their total amount after hydrolysis. Two MS/MS transitions were acquired for both compounds using the Q/q ratios as confirmatory parameters. Standard addition calibration curves demonstrated optimal linearity and negligible matrix effects, allowing a correct quantification even without expensive and difficult to find labeled internal standards. After several weeks of operation, the system’s repeatability was excellent, with an intraday RSD (%) spanning from five to nine and an interday RSD (%) spanning from 9 to 11.

Quantification and confirmation of four aflatoxins using a LC–MS/MS QTRAP system in multiple reaction monitoring, enhanced product ion scan, and MS3 modes

2019 ◽  
Vol 26 (1) ◽  
pp. 63-77
Author(s):  
Shencong Lv ◽  
Henghui Wang ◽  
Yong Yan ◽  
Miaohua Ge ◽  
Jian Guan
Keyword(s):  
Formic Acid ◽  
Ion Trap ◽  
Multiple Reaction Monitoring ◽  
Correlation Coefficients ◽  
Gradient Elution ◽  
Reaction Monitoring ◽  
Liquid Chromatography Tandem Mass ◽  
Multiple Reaction Monitoring Mode ◽  
Derivative Products

A simple, rapid, and efficient liquid chromatography tandem mass spectrometry (LC–MS/MS) method, operated in electrospray ionization and quadrupole linear ion trap modes, has been developed for the identification and structural characterization of aflatoxins in peanuts and its derivative products or bean sauce. Samples (5 g) were extracted with acetonitrile/water/formic acid (79:20:1, v/v). After centrifugation and dilution, the extracts were separated on a C18 analytical column by gradient elution (acetonitrile/0.2% formic acid) and analyzed by UPLC–MS/MS. External calibration was used for qualification. The developed multiple reaction monitoring–information-dependent acquisition–enhanced product ion method enabled quantification and confirmation of the analytes in a single run. Enhanced product ion mode was used for qualitative analysis, while multiple reaction monitoring mode was used for quantitative analysis. An in-house library was constructed for identification. Calibration curves showed good linearity with correlation coefficients (r) higher than 0.994. Limits of detection were determined to be below 0.26 µg kg−1 for most analytes. The recoveries for those substances were in the acceptable range of 80.2%–119.1%. A new LC–MS3 method was established for further confirmation. One pickled pepper peanut was found to contain aflatoxins B1, B2, and G1 with contents of 90.93, 26.64, and 1.92 µg kg−1, respectively.

Determination and validation of aprepitant in rat plasma using LC−MS/MS

Bioanalysis ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 363-372
Author(s):  
Nazlı Erdoğar ◽  
Tuba Reçber ◽  
Alper B İskit ◽  
Erem Bilensoy ◽  
Sedef Kır ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Formic Acid ◽  
Multiple Reaction Monitoring ◽  
Rat Plasma ◽  
Pharmacokinetic Parameters ◽  
Reaction Monitoring ◽  
Bioanalytical Method Validation ◽  
Bioanalytical Method ◽  
Acceptable Range ◽  
Multiple Reaction Monitoring Mode

Aim: The assessment of efficacy should be paralleled with extensive pharmacokinetic parameters, and a valid bioanalytical method is a pre-condition for accurate plasma concentration. Materials & methods: A simple, specific, rapid and sensitive LC−MS/MS method has been developed for quantitative analysis of aprepitant in rat plasma. A C18 column was used as stationary phase and the mobile phase consisted of a mixture of formic acid in water and formic acid in acetonitrile. Quantification was performed using multiple reaction monitoring mode. Results: The selectivity, linearity, accuracy, precision, robustness and ruggedness of the method were evaluated in accordance with bioanalytical method validation guideline of ICH and all results were within the acceptable range. Conclusion: The validated LC−MS/MS method was found to be useful for the quantitative analysis of aprepitant in rat plasma samples.

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