scholarly journals Impact of Three Different Light Spectra on the Yield, Morphology and Growth Trajectory of Three Different Cannabis sativa L. Strains

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1866
Author(s):  
Philipp Reichel ◽  
Sebastian Munz ◽  
Jens Hartung ◽  
Achim Präger ◽  
Stiina Kotiranta ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Plant Morphology ◽  
Cultivated Plants ◽  
Shade Avoidance ◽  
Light Spectra ◽  
Flower Yield ◽  
R:Fr Ratio ◽  
Lateral Branches ◽  
Different Strains ◽  
Changing Light

Cannabis is one of the oldest cultivated plants, but plant breeding and cultivation are restricted by country specific regulations. Plant growth, morphology and metabolism can be manipulated by changing light quality and intensity. Three morphologically different strains were grown under three different light spectra with three real light repetitions. Light dispersion was included into the statistical evaluation. The light spectra considered had an influence on the morphology of the plant, especially the height. Here, the shade avoidance induced by the lower R:FR ratio under the ceramic metal halide lamp (CHD) was of particular interest. The sugar leaves seemed to be of elementary importance in the last growth phase for yield composition. Furthermore, the last four weeks of flowering were crucial to influence the yield composition of Cannabis sativa L. through light spectra. The dry flower yield was significantly higher under both LED treatments compared to the conventional CHD light source. Our results indicate that the plant morphology can be artificially manipulated by the choice of light treatment to create shorter plants with more lateral branches which seem to be beneficial for yield development. Furthermore, the choice of cultivar has to be taken into account when interpreting results of light studies, as Cannabis sativa L. subspecies and thus bred strains highly differ in their phenotypic characteristics.

scholarly journals The Effect of Light Spectrum on the Morphology and Cannabinoid Content of Cannabis sativa L.

2018 ◽  
Vol 1 (1) ◽  
pp. 19-27 ◽  
Author(s):  
Gianmaria Magagnini ◽  
Gianpaolo Grassi ◽  
Stiina Kotiranta
Keyword(s):  
Light Intensity ◽  
Cannabis Sativa ◽  
Red Light ◽  
Total Yield ◽  
Plant Morphology ◽  
Dry Weight ◽  
Light Sources ◽  
Light Spectrum ◽  
Light Spectra ◽  
Generative Phase

Cannabis sativa L. flowers are the main source of Δ-9-tetrahydrocannabinol (THC) used in medicine. One of the most important growth factors in cannabis cultivation is light; light quality, light intensity, and photoperiod play a big role in a successful growth protocol. The aim of the present study was to examine the effect of 3 different light sources on morphology and cannabinoid production. Cannabis clones were grown under 3 different light spectra, namely high-pressure sodium (HPS), AP673L (LED), and NS1 (LED). Light intensity was set to ∼450 µmol/m2/s measured from the canopy top. The photoperiod was 18L: 6D/21 days during the vegetative phase and 12L: 12D/46 days during the generative phase, respectively. At the end of the experiment, plant dry weight partition, plant height, and cannabinoid content (THC, cannabidiol [CBD], tetrahydrocannabivarin [THCV], cannabigerol [CBG]) were measured under different light treatments. The experiment was repeated twice. The 3 light treatments (HPS, NS1, AP673L) resulted in differences in cannabis plant morphology and in cannabinoid content, but not in total yield of cannabinoids. Plants under HPS treatment were taller and had more flower dry weight than those under treatments AP673L and NS1. Treatment NS1 had the highest CBG content. Treatments NS1 and AP673L had higher CBD and THC concentrations than the HPS treatment. Results were similar between experiments 1 and 2. Our results show that the plant morphology can be manipulated with the light spectrum. Furthermore, it is possible to affect the accumulation of different cannabinoids to increase the potential of medicinal grade cannabis. In conclusion, an optimized light spectrum improves the value and quality of cannabis. Current LED technology showed significant differences in growth habit and cannabinoid profile compared to the traditional HPS light source. Finally, no difference of flowering time was observed under different R:FR (i.e., the ratio between red and far-red light).

scholarly journals Selective cannabis strain allergy in a patient presenting with a local allergic reaction

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Peter Stepaniuk ◽  
Amin Kanani
Keyword(s):  
Direct Contact ◽  
Cannabis Sativa ◽  
Skin Prick Testing ◽  
Case Presentation ◽  
Published Report ◽  
Cannabis Indica ◽  
Different Strains ◽  
Allergen Identification ◽  
Strain Dependent ◽  
Fresh Skin

Abstract Background Cannabis use is growing domestically due to recent legalization in many jurisdictions. There are two main species of cannabis, Cannabis sativa and Cannabis indica, and thousands of different commercially available cannabis strains. Although there are multiple reports of cannabis allergy in the literature, to our knowledge, there is no prior published report of selective cannabis strain allergy. Case presentation A 31-year-old male was referred for allergy assessment due to several episodes of localized pruritus and erythema after direct contact with various strains of cannabis. He had noted that the severity of his reaction appeared to be strain dependent. He developed a severe local reaction involving bilateral periorbital edema shortly after coming into direct contact with one particular strain of cannabis. He denied any adverse symptoms after inhalation of cannabis. Fresh skin prick testing was performed to various strains of cannabis and had positive testing to the three of the five tested strains. Conclusions We believe this is the first reported case of selective cannabis strain allergy based on patient history and skin prick testing. This case report outlines the variability in different strains of cannabis and stresses the importance of further research into cannabis allergen identification. Multiple cannabis allergens should be included and incorporated into commercial extracts when they become routinely available.

scholarly journals Yield, Characterization, and Possible Exploitation of Cannabis Sativa L. Roots Grown under Aeroponics Cultivation

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4889
Author(s):  
Fabio Ferrini ◽  
Daniele Fraternale ◽  
Sabrina Donati Donati Zeppa ◽  
Giancarlo Verardo ◽  
Andrea Gorassini ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Therapeutic Potential ◽  
Total Content ◽  
Bioactive Molecules ◽  
Bioactive Metabolites ◽  
Cultivated Plants ◽  
Root Extracts ◽  
Cultivation Technique ◽  
Long Time ◽  
Health Promoting

Cannabis sativa L. has been used for a long time to obtain food, fiber, and as a medicinal and psychoactive plant. Today, the nutraceutical potential of C. sativa is being increasingly reappraised; however, C. sativa roots remain poorly studied, despite citations in the scientific literature. In this direction, we identified and quantified the presence of valuable bioactives (namely, β-sitosterol, stigmasterol, campesterol, friedelin, and epi-friedelanol) in the root extracts of C. sativa, a finding which might pave the way to the exploitation of the therapeutic potential of all parts of the C. sativa plant. To facilitate root harvesting and processing, aeroponic (AP) and aeroponic-elicited cultures (AEP) were established and compared to soil-cultivated plants (SP). Interestingly, considerably increased plant growth—particularly of the roots—and a significant increase (up to 20-fold in the case of β-sitosterol) in the total content of the aforementioned roots’ bioactive molecules were observed in AP and AEP. In conclusion, aeroponics, an easy, standardized, contaminant-free cultivation technique, facilitates the harvesting/processing of roots along with a greater production of their secondary bioactive metabolites, which could be utilized in the formulation of health-promoting and health-care products.

scholarly journals Genetic Variation of ‘Candidatus Liberibacter solanacearum’ Haplotype C and Identification of a Novel Haplotype from Trioza urticae and Stinging Nettle

2018 ◽  
Vol 108 (8) ◽  
pp. 925-934 ◽  
Author(s):  
M. Haapalainen ◽  
J. Wang ◽  
S. Latvala ◽  
M. T. Lehtonen ◽  
M. Pirhonen ◽  
...  
Keyword(s):  
Wild Plants ◽  
Cultivated Plants ◽  
Group Method ◽  
Stinging Nettle ◽  
Arithmetic Means ◽  
Candidatus Liberibacter Solanacearum ◽  
Pair Group ◽  
Carrot Seed ◽  
Candidatus Liberibacter ◽  
Different Strains

‘Candidatus Liberibacter solanacearum’ (CLso) haplotype C is associated with disease in carrots and transmitted by the carrot psyllid Trioza apicalis. To identify possible other sources and vectors of this pathogen in Finland, samples were taken of wild plants within and near the carrot fields, the psyllids feeding on these plants, parsnips growing next to carrots, and carrot seeds. For analyzing the genotype of the CLso-positive samples, a multilocus sequence typing (MLST) scheme was developed. CLso haplotype C was detected in 11% of the T. anthrisci samples, in 35% of the Anthriscus sylvestris plants with discoloration, and in parsnips showing leaf discoloration. MLST revealed that the CLso in T. anthrisci and most A. sylvestris plants represent different strains than the bacteria found in T. apicalis and the cultivated plants. CLso haplotype D was detected in 2 of the 34 carrot seed lots tested, but was not detected in the plants grown from these seeds. Phylogenetic analysis by unweighted-pair group method with arithmetic means clustering suggested that haplotype D is more closely related to haplotype A than to C. A novel, sixth haplotype of CLso, most closely related to A and D, was found in the psyllid T. urticae and stinging nettle (Urtica dioica, Urticaceae), and named haplotype U.

scholarly journals Characterisation and evaluation of morphological trials, biological features and seed yield of 23 flax accessions (Linum usitatissimum L.) of different geographical origins

2018 ◽  
Vol 64 (4) ◽  
pp. 1-13 ◽  
Author(s):  
Grażyna Silska ◽  
Jan Bocianowski
Keyword(s):  
Genetic Resources ◽  
Seed Yield ◽  
Linum Usitatissimum ◽  
Biological Diversity ◽  
Cannabis Sativa ◽  
Field Trials ◽  
Convention On Biological Diversity ◽  
Biological Features ◽  
Linum Usitatissimum L ◽  
Lateral Branches

Summary Introduction: The Institute of Natural Fibres and Medicinal Plants (INF&MP) is involved in the protection of the genetic resources of flax (Linum usitatissimum L.) and hemp (Cannabis sativa L.). In 1998–2000, the INF&MP conducted research titled “Collection and evaluation of flax and hemp cultivars and ecotypes”. Poland participates in the programme, financed by the Ministry of Agriculture and Rural Development because our country has signed the Convention on Biological Diversity. Objective: The objective of the research was the evaluation of the genetic resources of 23 accessions of flax. The characteristics data for flax accessions are presented according to traditional methodology and according to the methodology for developing the International Flax Data Base. Methods: Field trials were carried out in 1998–2000 in the experimental station in Wojciechów, located in the Opolskie region. The objective was to evaluate the morphological traits, biological features and seed yield of 23 accessions of flax. The research included the following genotypes of Linum usitatissimum L. according to type of use: 12 linseed varieties, three landraces, one accession of unknown type of use, six fibre varieties and one genotype of both types of use. Results: The total plant length of Linum was short for 12 accessions of flax, medium short (7) and medium (4). The technical length was short (17 accessions), medium short (3) and medium (3). The stem thickness for all accessions was medium, i.e. between 1.2 and 2.0 mm. The panicle length was short (12 accessions), medium (10) and long (1). A number of primary lateral branches of the stems of all flax accessions was medium (4–8 branches). The 1000 seed weight was low for 21 accessions of flax and very low in two genotypes: Currong and UKR 97 104. The size of the corolla (flower) was small (9), medium (12) and large (2) – varieties: Martin and Maxigold. The petal colour of the corolla was blue (11), light blue (8) and white (4) – Crystal, Hella, Kreola, Achay. Conclusions: Morphological characterisation of the stamen showed that accession K-1390 was segregating because the anther colour was bluish and greish. The highest resistance to lodging was observed for Abby, Peak and Olinette. The following accessions of flax were very resistant to Fusarium: Martin, UKR 97269, Kijewskij and Ukraińskij 3; resistant to Fusarium: Abby, Peak, Olinette, Crystal, Gold Merchant, Currong and Ukraińskij 2. The following varieties were very susceptible to Fusarium: Hella, Kreola, Maxigold and Achay. These varieties should not be used for cross breeding. The best average seed yield in 1998–2000 in Wojciechów was found in the following accessions of flax: Abby (191%), Gold Merchant (162%), Currong (161%), Olinette (151%), PEAK (148%), UKR 97269 (148%), Crystal (138%), Ukraiński 3 (132%), Martin (130%), Pacific (110%) and Ukraiński 2 (103%). These varieties are particularly valuable for flax breeders.

VARIATIONS IN TERPENE PROFILES OF DIFFERENT STRAINS OF CANNABIS SATIVA L.

2011 ◽  
pp. 115-121 ◽  
Author(s):  
S. Casano ◽  
G. Grassi ◽  
V. Martini ◽  
M. Michelozzi
Keyword(s):  
Cannabis Sativa ◽  
Different Strains

scholarly journals Domestication reduces phenotypic plasticity in chaya (Cnidoscolus aconitifolius (Mill.) I.M. Johnst)

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Miguel A. Munguía-Rosas
Keyword(s):  
Phenotypic Plasticity ◽  
Artificial Selection ◽  
Stem Elongation ◽  
Light Availability ◽  
Wild Plants ◽  
Cultivated Plants ◽  
Phenotypic Traits ◽  
Shade Avoidance ◽  
Leaf Production ◽  
Cnidoscolus Aconitifolius

Background: Natural selection optimizes phenotypic plasticity in plants found in environmentally variable habitats. However, it is unclear how artificial selection has affected the phenotypic plasticity of crops. Reduced plasticity in crop yield is often considered a desirable feature in cultivated plants; however, limited phenotypic plasticity in this and other traits may also affect the ability of crops to cope with environmental variation. Study species: Wild and domesticated chaya (Cnidoscolus aconitifolius (Mill.) I.M. Johnst). Question: How domestication has affected the phenotypic plasticity of vegetative traits in response to the light environment? Methods: Leaf area, leaf perimeter, leaf specific area, leaf production, trichome density, stem elongation, growth in stem diameter and slenderness were measured in clones of wild and domesticated plants. These clones were allocated to two contrasting light treatments: fully exposed to sun vs. placement beneath a shade cloth. The phenotypic traits and reaction norms were compared between the genotypes of wild and domesticated plants. Results: Lower plasticity in leaf production and slenderness was observed in the domesticated compared to the wild plants. Leaf production and slenderness are associated with the shade avoidance syndrome, which was evident in wild plants but not manifested in domesticated plants. Reduced plasticity in leaf production also suggests yield stability. Conclusion: Artificial selection reduces phenotypic plasticity in the yield of chaya and in its response to variation in light availability.

scholarly journals Quantitative Determination of Δ9-THC, CBG, CBD, Their Acid Precursors and Five Other Neutral Cannabinoids by UHPLC-UV-MS

Planta Medica ◽  
2017 ◽  
Vol 84 (04) ◽  
pp. 260-266 ◽  
Author(s):  
Yan-Hong Wang ◽  
Bharathi Avula ◽  
Mahmoud ElSohly ◽  
Mohamed Radwan ◽  
Mei Wang ◽  
...  
Keyword(s):  
Formic Acid ◽  
Quantitative Determination ◽  
Cannabis Sativa ◽  
Solvent System ◽  
Plant Sample ◽  
Relative Standard ◽  
Different Strains ◽  
Processing And Storage ◽  
Liquid Chromatographic

AbstractCannabinoids are a group of terpenophenolic compounds in the medicinal plant Cannabis sativa (Cannabaceae family). Cannabigerolic acid, Δ9-tetrahydrocannabinolic acid A, cannabidiolic acid, Δ9-tetrahydrocannabinol, cannabigerol, cannabidiol, cannabichromene, and tetrahydrocannabivarin are major metabolites in the classification of different strains of C. sativa. Degradation or artifact cannabinoids cannabinol, cannabicyclol, and Δ8-tetrahydrocannabinol are formed under the influence of heat and light during processing and storage of the plant sample. An ultrahigh-performance liquid chromatographic method coupled with photodiode array and single quadruple mass spectrometry detectors was developed and validated for quantitative determination of 11 cannabinoids in different C. sativa samples. Compounds 1 – 11 were baseline separated with an acetonitrile (with 0.05% formic acid) and water (with 0.05% formic acid) gradient at a flow rate of 0.25 mL/min on a Waters Cortec UPLC C18 column (100 mm × 2.1 mm I. D., 1.6 µm). The limits of detection and limits of quantitation of the 11 cannabinoids were below 0.2 and 0.5 µg/mL, respectively. The relative standard deviation for the precision test was below 2.4%. A mixture of acetonitrile and methanol (80 : 20, v/v) was proven to be the best solvent system for the sample preparation. The recovery of all analytes was in the range of 97 – 105%. A total of 32 Cannabis samples including hashish, leaves, and flower buds were analyzed.

scholarly journals Strain-, Sex-, and Time-Dependent Antidepressant-like Effects of Cannabidiol

2021 ◽  
Vol 14 (12) ◽  
pp. 1269
Author(s):  
Gabriela P. Silote ◽  
Michelle C. Gatto ◽  
Amanda Eskelund ◽  
Francisco S. Guimarães ◽  
Gregers Wegener ◽  
...  
Keyword(s):  
Selective Breeding ◽  
Cannabis Sativa ◽  
Tail Suspension Test ◽  
Rodent Models ◽  
Tail Suspension ◽  
Resistant Lines ◽  
Antidepressant Effects ◽  
Immobility Time ◽  
Administration Time ◽  
Different Strains

Cannabidiol (CBD) is a non-intoxicating compound extracted from Cannabis sativa, showing antidepressant-like effects in different rodent models. However, inconsistent results have been described depending on the species and the strain used to assess depressive-like behavior. Moreover, only a few studies investigated the effect of CBD in female rodents. Therefore, we aimed to (i) investigate the effects of CBD in two different strains of mice (Swiss and C57BL/6) and a rat model of depression based on selective breeding (Flinders Sensitive and Resistant Lines, FSL and FRL) subjected to tests predictive of antidepressant-like effects and (ii) investigate the influence of sex in the effects of CBD in both mice and rats. CBD induced an antidepressant-like effect in male Swiss but not in female Swiss or C57BL/6 mice in the tail suspension test (TST). In male FSL rats, CBD produced an antidepressant-like effect 1 h post injection. However, in female FSL, CBD induced a bimodal effect, increasing the immobility time at 1 h and decreasing it at 2 h. In conclusion, strain, sex, and administration time affect CBD’s behavioral response to rodents exposed to tests predictive of antidepressant effects.

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