Culture medium for mint micropropagation in vitro

Clonal Micropropagation of representatives of the genus Dactylorhiza Neck. ex Nevski

АгроЭкоИнфо ◽

10.51419/20214417 ◽

2021 ◽

Vol 4 (46) ◽

pp. 17-17

Author(s):

Alexander Saakian ◽

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Keyword(s):

Survival Rate ◽

Growth Regulators ◽

In Vitro Propagation ◽

Culture Medium ◽

Culture Media ◽

Ms Medium ◽

Organic Additives ◽

Clonal Micropropagation ◽

Half Strength

Abstract The aim of this study is to develop and improve methods of in vitro propagation of representatives of Dactylorhiza: D.baltica , D. fuchsii. For the study, we used protocorms obtained by the asymbiotic germination of seed during 90 days. It has been established that half-strength of Murashige and Skoog (1962) medium (½ MS) supplemented with 1-2 mg/l 6-Benzylaminopurine(6-BAP), potato puree (20g/l), and charcoal (1g/l) effectively influenced the development of protocorms, and seedlings formation in the studied species. The result of the study showed that the survival rate of protocorms was high in all experimental culture media, but in D. fuchsii it was better at a concentration 2mg/l of 6-BAP (95.4%), while in D. baltica it was high at 1mg/l (87.0%). The highest percentage of multiple protocorms (68%) and the formation of new secondary protocorms in D. fuchsii (5,5±0,3 units) were observed on a culture medium containing 2 mg/l 6-BAP. The highest percent of rooting of D. fuchsii protosoms (78%) and length of roots (0.9cm) observed in ½ MS medium without growth regulators. During the development of D. baltica protosoms, the culture medium of ½ MS containing 1 mg/l 6-BAP had the best effect on the number of roots (1.8±0.1root/protosom), while the medium supplemented with 2mg/l of 6-BAP contributed to the formation of a larger number of new secondary protocorms (3,2±0,1protocorm/unit). During the subsequent cultivation of protosoms of D. baltica on a culture medium containing 1 mg/l it was observed an increase in the height of shoots (4,8±0,3 см), and the length of roots (2,2±0,1 см), wherein the number of newly formed protocorms was higher by 30% on the medium supplemented with 2 mg/l 6-BAP. Keywords: DACTYLORHIZA BALTICA, DACTYLORHIZA FUCHSII, IN VITRO, PROTOCORMS, ORGANIC ADDITIVES

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Peculiarities of Thymus tauricus Klokov et Des.-Shost. clonal micropropagation

PROCEEDINGS OF V INTERNATIONAL SCIENTIFIC CONFERENCE “CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE” ◽

10.33952/2542-0720-2020-5-9-10-97 ◽

2020 ◽

Author(s):

A.Sh. Tevfik ◽

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N.A. Yegorova ◽

Keyword(s):

Culture Medium ◽

Medium Composition ◽

Ms Medium ◽

Optimal Composition ◽

Cultivation Conditions ◽

Second Stage ◽

Clonal Micropropagation

The aim of the investigation was to study the influence of cultivation conditions and the culture medium composition on the Thymus tauricus Klokov et Des.-Shost explants morphogenesis at the 1st-2nd stages of clonal micropropagation. The optimal composition of culture medium at the introduction stage is the MS medium with 1.0 mg/l of kinetin and 1.0 mg/l GА3. To obtain a high multiplication index (29.4) at the second stage of micropropagation, it is necessary to cultivate explants in flasks with MS medium supplemented with 1.0 mg/l kinetin.

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Biochemical and morpho-anatomical analyses of strawberry vitroplants hyperhydric tissues affected by BA and gelling agents

Revista CERES ◽

10.1590/s0034-737x2013000200002 ◽

2013 ◽

Vol 60 (2) ◽

pp. 152-160 ◽

Cited By ~ 5

Author(s):

Leticia Mascarenhas Pereira Barbosa ◽

Vespasiano Borges de Paiva Neto ◽

Leonardo Lucas Carnevalli Dias ◽

Reginaldo Alves Festucci-Buselli ◽

Rodrigo Sobreira Alexandre ◽

...

Keyword(s):

In Vitro Propagation ◽

Large Scale ◽

Culture Medium ◽

Shoot Proliferation ◽

Cellular Level ◽

Fragaria X Ananassa ◽

Scale Production ◽

Ms Medium ◽

Large Scale Production

In vitro propagation has become an effective practice for large-scale production of strawberry plants. The objective of this study was to evaluate the hyperhydricity and the multiplication capacity of two strawberry varieties (Fragaria x ananassa Duch. 'Dover' and 'Burkley') propagated in vitro. Plants maintained in MS medium supplemented with 1.0 mg L-1 BA were individualized and transferred to the same medium solidified with Agar (6.5 g L-1) or Phytagel® (2.5 g L-1) and BA at different concentrations (0; 0.5; 1.0; 2.0 and 3.0 mg L-1). Biochemical and anatomical analyses were carried out, as well as the analysis of the morphological hyperhydricity characteristics. The analysis of data showed: a) the increase in cytokinin concentration increased hyperhydricity frequency in both varieties; b) at concentrations up to 2.0 mg L-1 BA, the replacement of Agar by Phytagel® induced a higher formation of hyperhydric shoots; and c) the addition of BA induced oxidative stress, which is characterized by increased antioxidant activity and lipid peroxidation, as well as alterations at the cellular level, such as malformation of stomata and epidermal cells. In conclusion, the culture medium containing 0.5 mg L-1 BA solidified with Agar provided lower hyperhydricity percentages in association with higher rates of shoot proliferation in strawberry.

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In vitro Propagation of Bacopa monnieri (L.)

Biotechnology Journal International ◽

10.9734/bji/2020/v24i430111 ◽

2020 ◽

pp. 32-39

Author(s):

Poornima Raj ◽

J. Anbumalarmathi ◽

S. Aruna Sharmili

Keyword(s):

Survival Rate ◽

Growth Regulators ◽

In Vitro Propagation ◽

Bacopa Monnieri ◽

Shoot Length ◽

Ms Medium ◽

Root Initiation ◽

Shoot Induction ◽

Propagation Technique

An experiment was conducted for standardization of in vitro propagation technique of Bacopa monnieri (L.), a medicinal herb of India. Healthy leaf segments of the herb were used as explants with basic Murashige and Skoog (MS) medium containing various combinations of different growth regulators for callus, shoot and root initiation. The best callus induction percentage (95.47%) was observed on MS + 0.5 mg/L NAA and 2.0 mg/L BAP (T3). The maximum number of shoots (8), shoot length (9.30 cm) and shoot induction percentage (90.48%) was achieved on MS + 3.0 mg/L BAP and 1.0 mg/L Kn (ST4). The maximum number of roots (8) and root length (7) was observed on MS + 1.5 mg/L IAA (RT5). The rooted micro shoots were successfully hardened and acclimatized in green house and subsequently established in soil with survival rate of 90%.

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Propagation Methods of Yam (Dioscorea Species) with Special Attention to In Vitro Propagation

Journal of Applied Biotechnology ◽

10.5296/jab.v4i1.9031 ◽

2016 ◽

Vol 4 (1) ◽

pp. 13

Author(s):

Obssi Dessalegn

Keyword(s):

In Vitro Propagation ◽

Multiplication Rate ◽

Regeneration Rate ◽

Seed Tubers ◽

High Regeneration ◽

Tuber Crop ◽

Dioscorea Species ◽

Propagation Methods ◽

Propagation Technique

Yam is a monocotyledonous plant in the genus Dioscorea. It is a multi-species tuber crop cultivated in Africa, Asia and parts of South America. Yam is an important crop in South and Southwestern parts of Ethiopia. Many species of Dioscorea genus are economically important crops and many of them have been used in the pharmaceutical industry. Yam is propagated from seed tubers or sections of tuber and corms. Seed tubers are expensive, bulky to transport and the multiplication rate in the field is very low. Shortage of seed tubers for planting is one of the major constraints for yam production in Ethiopia. To overcome such problems and to increase production, different propagation methods have been implemented for many Dioscorea species. Convectional and In vitro propagation of Dioscorea species pave the way to meet the demand of this economically important plant. The protocols are designed to provide the optimal levels of mineral nutrients, environmental factors, vitamins and carbohydrates to achieve the high regeneration rate of the different species of Dioscorea in vitro. This review summarizes some of the important reports on different propagation technique of Dioscorea from the literature data.

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Asymbiotic seed germination and in vitro propagation of Cattleya trianae Linden & Reichb.f. (Orchidaceae)

Acta Agronómica ◽

10.15446/acag.v66n4.63597 ◽

2017 ◽

Vol 66 (4) ◽

pp. 544-548 ◽

Cited By ~ 7

Author(s):

Seir Antonio Salazar Mercado ◽

Nelson Alfonso Vega Contreras

Keyword(s):

Seed Germination ◽

Threatened Species ◽

Growth And Development ◽

In Vitro Propagation ◽

Culture Medium ◽

Ms Medium ◽

Organic Additives ◽

Natural Habitats ◽

Asymbiotic Seed Germination

Cattleya trianae (Linden & Reichb.f., 1860), Colombian national flower, is in danger of extinction due to the destruction of its natural habitats and excessive collection for horticultural purposes. Therefore, in vitro culture is a tool for the conservation of threatened species. In this study we determined the most suitable culture medium for asimbytic seed germination and in vitro propagation of C. trianae. Initially, mature capsules were collected, the seeds were subsequently disinfected and seeded with the syringe method (Vendrame et al., 2007), to evaluate the effect of five media on the development of C. trianae after 20 weeks. The seedlings were transplanted and acclimated using different substrates. The best percentage (54.2%) of seedling formation after 20 weeks was found in MS + JP medium with significant differences (P <0.05: Tukey HSD). In this research, it is reported that the addition of organic additives to the MS medium improves the efficacy of this, and therefore, allows a greater growth and development of C. trianae under in vitro conditions.

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Optimization of clonal micropropagation of Chrysanthemum

Ukrainian Journal of Ecology ◽

10.15421/2019_809 ◽

2019 ◽

Vol 9 (4) ◽

pp. 676-678

Author(s):

I. D. Borodulina ◽

A. Trufanova ◽

G. Shevchenko ◽

G. Sokolova ◽

T. Plaksina

Keyword(s):

Growth Regulators ◽

Traditional Method ◽

Proliferative Activity ◽

Culture Medium ◽

Ms Medium ◽

Mineral Salts ◽

Clonal Micropropagation ◽

Murashige Skoog ◽

High Proliferative Activity

Micropropagation of Chrysanthemum is an alternative to the traditional method of reproduction. Thanks to this method, the Chrysanthemum reproduction time is reduced to 3-4 months. For clonal micropropagation, sterile microshoots of Chrysanthemums of the varieties Snow White, Stranger, and Baltica White were used. At the stage of the micropropagation, the Murashige-Skoog (MS) medium with the full and half composition of mineral salts and growth regulators (kinetin, 6-benzylaminopurine, β-indolylacetic acid) were used. A universal culture medium for clonal micropropagation of all varieties of Chrysanthemum and optimal mediums, taking into account variety-specificity were established. It was noted that under in vitro conditions, high proliferative activity was observed in Neznakomka variety.

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Clonal micropropagation of Thymus vulgaris L.

E3S Web of Conferences ◽

10.1051/e3sconf/202022404001 ◽

2020 ◽

Vol 224 ◽

pp. 04001

Author(s):

A Sh Tevfik ◽

N. A. Yegorova

Keyword(s):

Plant Material ◽

Culture Medium ◽

Culture Media ◽

Morphometric Parameters ◽

Thymus Vulgaris ◽

Ms Medium ◽

Ex Vitro ◽

Clonal Micropropagation ◽

Propagation Stage

Thymus vulgaris L. is one of the widely known spicy aromatic and medicinal plants. Thyme plant material is widely used in medicine, cooking and perfumery. To increase the efficiency of breeding and seed production, it is necessary to develop biotechnological techniques, in particular, clonal micropropagation. The aim of the research is to optimize the composition of culture media for the main stages of propagation in vitro and to select adaptation ex vitro conditions for the development of Thymus vulgaris. clonal micropropagation. The article presents the results of studies of explant morphometric parameters cultivated on 20 variants of culture media at firstsecond stages of micropropagation. It was found that the optimal culture medium at the introduction stage is MS medium with 1.0 mg/l Kin and 1.0 mg/l GA3, on which, on average, 2.2 microshoots per explant with a length of 1.9 cm were obtained. Both high vitrification rate of microshoots and formation of small shoots (0.6-0.9 cm) were observed on media supplemented with BAP or TDZ. The most effective culture medium at the proper propagation stage is MS with 1.0 mg/l Kin, on which 4.6 shoots per explant and the multiplication index 12.8 were obtained. It is advisable to root microshoots at the 3rd stage of micropropagation on MS culture medium supplemented with 1.0 mg/l IBA or 1.0 mg/l IAA. It has been shown that it is possible to obtain high plant survival rate (89.5%) during adaptation ex vitro, using a substrate consisting of peat and perlite (1:1).

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Micropropagation in vitro of essential oil rose hybrids obtained in embryoculture

BIO Web of Conferences ◽

10.1051/bioconf/20213800139 ◽

2021 ◽

Vol 38 ◽

pp. 00139

Author(s):

Natalia Yegorova ◽

Irina Stavtzeva ◽

Victor Zolotilov

Keyword(s):

Essential Oil ◽

Embryo Culture ◽

Interspecific Hybrids ◽

Culture Medium ◽

High Variability ◽

Ms Medium ◽

Clonal Micropropagation ◽

Hybrid Seedling ◽

Stem Segments

The aim of the work was to study the features of clonal micropropagation of essential oil rose interspecific hybrids obtained in embryo culture in vitro. Analysis of 12 crossing combinations demonstrated that the frequency of hybrid seedling formation in the embryo culture varied from 0 to 71.4%. For clonal micropropagation obtained in vitro seedlings were divided into stem segments with a node and cultivated on MS culture medium supplemented with 0.5 mg/l BAP and 0.1 mg/l GA3. During the multiplication of 13 hybrids (R. alba × R. damascena cv. ‘Kazanlykskaya’) in 2-6 subcultures, high variability of the multiplication index (1.8-18.5 depending on the genotype and passage) was revealed. This parameter was maximum in the 3-4th subcultures. The best ability to micropropagation showed hybrid No. 37-14. Microshoots were rooted in vitro on ½ MS medium, containing for different hybrids 0.5 or 1.0 mg/l NAA; frequency – up to 80.5-100.0%. However, in No. 37-2, 37-19 and 37-31 on four tested media, the number of shoots with roots was only 0-35.4%.

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Multiplicação de anador (Justicia pectoralis) in vitro

Revista Verde de Agroecologia e Desenvolvimento Sustentável ◽

10.18378/rvads.v11i3.3986 ◽

2016 ◽

Vol 11 (3) ◽

pp. 159 ◽

Cited By ~ 1

Author(s):

Rômulo Magno Oliveira Freitas ◽

Narjara Walessa Nogueira ◽

Sidney Carlos Praxedes

Keyword(s):

Plant Material ◽

In Vitro Propagation ◽

Culture Medium ◽

Culture Media ◽

Statistical Design ◽

The Other ◽

Nodal Segments ◽

Ms Medium ◽

Number Of Leaves

O trabalho teve como objetivo desenvolver um protocolo de micropropagação de segmentos nodais de anador (Justicia pectoralis). Para isso foram realizados dois experimentos. O delineamento estatístico utilizado foi o inteiramente casualizado, com 15 repetições. Os segmentos de J. pectoralis, após desinfestados, foram cultivados em meio MS durante 30 dias. No primeiro experimento, esse material foi repicado em três meios de cultura (MS, WPM e B5) e após 77 dias foram avaliados comprimento de plântula, número de raízes, número de folhas e o número de segmentos nodais. Para o segundo experimento foram testadas duas citocininas (BAP e Cinetina) nas seguintes dosagens 0,0; 0,5; 5,0 e 20,0 mM. Aos 60 dias após a repicagem foram avaliadas as seguintes características: números de folhas, número de raízes e número de explantes por planta. O meio MS foi o que apresentou maior comprimento de plântula. As demais variáveis não diferiram entre os meios utilizados. Por isso o meio MS foi utilizado para o segundo experimento onde se verificou que a utilização de BAP proporcionou maior número de folhas e de explantes quando submetido à concentração de 20 mM. Dessa forma, para multiplicação de seg Justicia pectoralis, recomenda-se a utilização de meio MS com adição de 20mM de BAP.In vitro propagation of Justicia pectoralisAbstract: The study aimed to establish a micropropagation protocol for Justicia pectoralis nodal segments. Two experiments were conducted. The statistical design was the completely randomized with 15 repetitions. After disinfestation, the segments of J. pectoralis were inoculated in the MS culture medium for 30 days. In the first experiment, the plant material was transferred to three culture media (MS, WPM and B5). The length of seedlings, number of roots, number of leaves, and number of nodal segments were evaluated at 77 days after transferring. In the second experiment two cytokinins (BAP and Kinetin) were tested in the following concentrations: 0.0; 0.5; 5.0 and 20.0 mM. At 60 days after transplanting the number of leaves, number of roots and number of explants per plant were evaluated. The MS medium induced the highest length of seedlings, but there was no effect for the other variables. Therefore, this medium was used for the second experiment, when it was found that BAP induced a larger number of leaves and explants when applied at 20 mM. Therefore, for multiplying J. pectoralis nodal segments we recommend the use of MS medium with 20 mM BAP.

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