scholarly journals Histological Changes in the Kidneys and Heart in Experimental Acanthamoebiasis in Immunocompetent and Immunosuppressed Hosts

2021 ◽  
Vol 69 (4) ◽  
pp. 167-178
Author(s):  
Karolina Kot ◽  
Danuta Kosik-Bogacka ◽  
Łanocha-Arendarczyk ◽  
Michał Ptak ◽  
Paulina Roszkowska ◽  
...  
Keyword(s):  
Immune Status ◽  
Heart Weight ◽  
Organ Specificity ◽  
Atypical Pneumonia ◽  
Histopathological Changes ◽  
Histological Changes ◽  
Protein Expressions ◽  
Duration Of Infection ◽  
Induced Changes ◽  
Immunosuppressed Mice

The course of Acanthamoeba spp. infection depends on the age and immune status of the host, and the virulence of the Acanthamoeba spp. strain. Some strains of free-living amoebae exhibit organ specificity, during the course of infection, while others may cause changes in many organs or completely lose pathogenicity. The aim of the current study was to investigate the pathological properties of Acanthamoeba spp. isolated from a patient with acute myeloid leukemia and atypical pneumonia (AM22). Moreover, the objective was to investigate the histopathological changes in the kidneys and heart of immunocompetent and immunosuppressed mice infected with Acanthamoeba spp. Amoebae were re-isolated from both the kidneys and hearts of the inoculated mice, although no cysts or trophozoites of the amoebae were detected in microscopic slides of the fragments of these organs. Acanthamoeba spp. induced changes in the kidney and heart weight of infected mice. In immunocompetent and immunosuppressed Acanthamoeba spp. infected mice, we found some histopathological changes, including areas with less acidic cytoplasm and a relaxation of muscle fibers. In further studies, it is important to analyze changes in gene and protein expressions in the heart and kidneys of hosts with disseminated acanthamoebiasis to better understand the course of infection in these organs, because the results of histological analysis varied depending on the immune status and duration of infection.

A Computerized Coding System for Processing Basic Histopathological Changes – Application to Vascular Pathology

1986 ◽  
Vol 25 (03) ◽  
pp. 139-142 ◽  
Author(s):  
A. Mauriello ◽  
Y. Sambuy ◽  
E. Bonanno ◽  
A. Orlandi ◽  
G. Palmieri ◽  
...  
Keyword(s):  
Vascular Wall ◽  
Final Diagnosis ◽  
Vascular Pathology ◽  
Histopathological Study ◽  
Coding System ◽  
Histopathological Changes ◽  
Histological Changes ◽  
Pathological Conditions ◽  
Computer Based ◽  
Sufficient Space

SummaryAmong the numerous existing computer-based systems for processing pathological data, none contains sufficient space for encoding data on the basic cytological or histological changes of a certain organ or tissue, upon which the final diagnosis is based.An “analytical record” was constructed listing all the basic changes that can be encountered in the various pathological conditions of the vascular wall. The data collected on the “analytical record” were coded by means of an alphanumeric code and stored in an Apple II 48 K minicomputer.The advantages of this system include the computerization of the data by non-specialized personnel and the possibility to’ quantitatively analyze the histocytopathological parameters used for diagnosis in vascular pathology. This coding system may easily be adapted, with minor modifications, to the histopathological study of other organs and tissues.

Toxic effects of sub lethal concentration of dioctyl phthalate on the histology of liver of Clarias batrachus (Linn.).

2016 ◽  
Vol 5 (09) ◽  
pp. 4874
Author(s):  
Manisha Satpathi* ◽  
Ravinder Singh
Keyword(s):  
Acute Toxicity ◽  
Light Microscopy ◽  
Dioctyl Phthalate ◽  
Clarias Batrachus ◽  
Fresh Water Fish ◽  
Histopathological Changes ◽  
Histological Changes ◽  
Water Fish ◽  
Toxicity Effects ◽  
Plastic Products

Dioctyl phthalate (DOP) commonly used as plasticizer enters into aquatic environment from the industries manufacturing plastic products, PVC resins, cosmetics and many other commercial products. Therefore, this study was designed to evaluate the acute toxicity effects of Dioctyl phthalate on fresh water fish Clarias batrachus. The 96 hour LC50 of Dioctyl phthalate in Clarias batrachus was estimated as 14.5ml/L. Histopathological changes in liver of Clarias batrachus were determined by exposing them to a fraction of LC50(1/5th) dose with every change of water for 30 days.The liver of Clarias batrachus was excised at every interval of 30 days and histological changes of liver were studied under light microscopy. Liver tissues showed abnormalities. Centrilobular vacuolation, necrosis, eccentric nuclei and enlarged nuclei, Centrilobular degeneration of hepatocytes were observed in liver tissue of fish.

scholarly journals HISTOLOGIC AND RADIOGRAPHIC STUDY OF PATHOLOGIC CHANGE IN COMPLETE IMPACTED THIRD MOLARS DENTAL FOLLICLES

2021 ◽  
Author(s):  
Shafiqa Mohammed Haidra Masdoose ◽  
Akram Thabet Nasher ◽  
Monya A. El-Zine ◽  
Ameen Abdullah Yahya Al-Akwa ◽  
Hassan Abdulwahab Al-Shamahy ◽  
...  
Keyword(s):  
Peer Review ◽  
Dentigerous Cyst ◽  
Third Molar ◽  
Inflammatory Cells ◽  
The United States ◽  
Age Group ◽  
Histopathological Changes ◽  
Histological Changes ◽  
Impacted Third Molar ◽  
Younger Age

Background: Prophylactic extraction of the asymptomatic impacted third molar is routinely practiced in Europe and the United States. The justification for prophylactic extraction includes the need to reduce the risk of pathologic changes such as cysts and tumors.   Objectives: This study aimed to study the histological and radiological changes in the tooth follicles of upper and lower complete impacted 3rd molars -which appeared radiologically normal. Material and method: A prospective study included fifty patients aged 20 years and above who were referred to the Oral Surgery Clinic, Faculty of Dentistry, University of Sana'a. Patients had follicular space between (2.5mm -3mm) as measured by the panoramic X-ray. These teeth were removed surgically and the follicle was sent for histopathological examination. Results: Most histopathological changes were in dental follicles with a size of <2.5 mm (86%), and only 14% with 2.5 mm - 3 mm. There was statistical significance between the smallest size of dental follicles with the incidence of pathological histological changes indicating a high probability of developing neoplasm (p =0.008).  Of the 50 follicular patients, 28% showed HC, nine (64%) had ameloblastoma, four (29%) had a dentigerous cyst, and only one case (7%) had a multicalcified focus with islands of odontogenic epithelium. While 72% of the samples had normal follicles and non-specific chronic inflammatory cells. There is an association between female sex and pathological histological changes (12 females: 2 males, p =0.008), age group 21-25 years (93% HC), with mandibles (65% HC). Regarding angle and histopathological changes, 36% were vertical, 29% mesioangular, 14.2% horizontal and destioangular, and 7.1% buccoangular. Conclusion:  High incidence of HC occurred in patients with DF, and it was associated with smaller dental follicle size, most HC was ameloblastoma, followed by dentigerous cyst, while 72% of samples had normal follicles and non-specific chronic inflammatory cells. There is a correlation between female gender, younger age group, and jaw position with HC. Prophylactic extraction of the asymptomatic impacted third molar should be routinely practiced in Yemen, to reduce the risk of pathological changes, especially in females and younger age groups.                            Peer Review History: Received 11 January 2021; Revised 8 February; Accepted 28 February, Available online 15 March 2021 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.  Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 5.5/10 Average Peer review marks at publication stage: 7.5/10 Reviewer(s) detail: Dr. A.A. Mgbahurike, University of Port Harcourt, Nigeria, [email protected] Dr. Alfonso Alexander Aguileral, University of Veracruz,  Mexico, [email protected]   Similar Articles: RADIOGRAPHIC ASSESSMENT OF THE COURSE AND VISIBILITY OF THE MANDIBULAR CANAL BY PANORAMIC RADIOGRAPHY

scholarly journals Characterization and Frequency of Histological Changes in Bovine Livers from Slaughterhouses in Brazil kept on Brachiaria spp.

2017 ◽  
Vol 45 (1) ◽  
pp. 9
Author(s):  
Rebekah Rank Araújo ◽  
Rayane Chitolina Pupin ◽  
Renata Cunha Madureira ◽  
Luiz Gustavo de Morais ◽  
Lucas De Souza Quevedo ◽  
...  
Keyword(s):  
Bile Ducts ◽  
Rio Grande ◽  
Liver Parenchyma ◽  
Rio Grande Do Sul ◽  
Histopathological Changes ◽  
Steroidal Saponins ◽  
Histological Changes ◽  
Mato Grosso ◽  
Native Pastures ◽  
Foamy Macrophages

Background: Brachiaria spp. is the main pasture for ruminant productions in Brazil, but the limiting factor for its use is the toxicity due to the presence of steroidal saponins. Chronic ingestion of Brachiaria spp. by cattle may cause liver changes such as fibrosis, bile duct proliferation and clusters of foamy macrophages in the hepatic parenchyma. The main objective of the present study was to evaluate the most frequent histological changes and their frequencies in livers collected in abattoirs in Brazil from beef cattle raised exclusively on Brachiaria spp. and compare them with those observed in animals kept in Andropogon spp. grass and native pastures in Rio Grande do Sul.Materials, Methods & Results: Liver samples without macroscopic changes were collected in abattoirs from 561 healthy Nelore and Nelore crossbred cattle raised in Brachiaria spp. pastures from Mato Grosso (MT), Mato Grosso do Sul (MS), Minas Gerais (MG) and Pará (PA) States. Liver samples from 84 Angus cattle (Bos taurus) kept on native pastures in Rio Grande do Sul (RS) and from 60 Nelore and Nelore crossbreed cattle raised in Andropogon spp. pastures in Tocantins State (TO) were collected as control. Semi-quantitative analysis of the histopathological changes were proceeded: (-) = no change; (+) = slight or discreet change; (++) = moderate change and (+++) = marked change. The main histopathological changes evaluted in the liver samples were the presence of foamy macrophages and its location, parenchymal fibrosis and its location, proliferation of bile ducts, periportal mononuclear infiltrate, presence of crystals in macrophages and within the bile ducts. Foamy macrophages clusters in the liver parenchyma were the most frequent histological changes exclusively observed in samples of animals kept in Brachiaria spp. and it was more frequent in cattle from MS State (P < 0.0001). The periportal hepatic fibrosis was another relevant finding in cattle kept in Brachiaria spp. and was also more frequent in MS animals (P ≤ 0.0001), and was not present in control groups. Proliferation of the bile ducts rate was similar between animals raised in Brachiaria spp. (P > 0.05), though it was higher in cattle kept on native pasture in RS (P < 0.0001). The mononuclear periportal inflammatory infiltrate was more frequent in MS and MG samples than in MT and PA (P < 0.0002).Discussion: The most significant histopathological changes observed in liver samples of cattle from the states of MT, MS, PA and MG kept in Brachiaria spp. pastures from the birth to slaughter, was the presence of foamy macrophages in quantities and variables distribution showing the strengthen association between clusters of foamy macrophages in the liver parenchyma and grazing on this grass. These macrophages could be found in healthy animals kept in Brachiaria spp. pastures and in intoxicated animals. This change was considered frequent and characteristic in animals kept in pastures containing steroidal lithogenic saponins in its composition, and it was frequently observed in ruminants that growed in Brachiaria spp. pastures. Beef cattle kept in Andropogon spp. grass pastures in the state of Tocantins and native pastures in Rio Grande do Sul did not showed foamy macrophages in liver samples. On the other hand, in susceptible sheep kept in Andropogon spp. grass pastures that  contains low amount of lithogenic steroidal saponins, are insufficient to induce toxicity and morphological changes in the liver, including aggregates of foamy macrophages.

scholarly journals Arsenic induced genotoxic and histopathological changes in male Swiss albino mice, Mus musculus

2011 ◽  
Vol 3 (2) ◽  
pp. 329-339
Author(s):  
Animesh K. Mohapatra ◽  
Deepika Rai ◽  
Anika Tyagi
Keyword(s):  
Arsenic Trioxide ◽  
Mus Musculus ◽  
Seminiferous Tubules ◽  
Histopathological Changes ◽  
Histological Changes ◽  
Hydropic Degeneration ◽  
Swiss Albino Mice ◽  
Cytoplasmic Vacuolization ◽  
Liver And Kidney ◽  
Albino Mice

The present study was carried out to investigate the effect of arsenic trioxide on the DNA and histomorphology of testis, liver and kidney of Swiss albino mice, Mus musculus. Oral administration of arsenic trioxide induced DNA damage in the testis, liver and kidney marked by light pink staining of nuclei after Feulgen’s reaction with reduced fine chromatin. Simultaneously severe histological changes were noted like distortion of seminiferous tubules, disorganization of spermatogonia, spermatocytes and spermatids with cytoplasmic vacuolization and nuclear pycnosis in testis. There was almost disappearance of sinusoids due to disruption of hepatic plates, inflammatory cellular infiltration around central veins and cytoplasmic vacuolization in hepatocytes with large irregular nuclei in liver of treated mice. Disorganized glomeruli with distorted Bowman’s capsules and mild to severe multifocal cloudy and hydropic degeneration with necrosis of tubules were observed in the kidney of treated mice. Inference drawn from the study indicated that arsenic induced both genotoxic histotoxic lesions.

scholarly journals Effect of Irbesartan on AGEs-RAGE and MMPs systems in rat type 2 diabetes myocardial-fibrosis model

2019 ◽  
Vol 244 (7) ◽  
pp. 612-620 ◽  
Author(s):  
Ye Hongwei ◽  
Cao Ruiping ◽  
Fang Yingyan ◽  
Zhang Guanjun ◽  
Hu Jie ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Body Weight ◽  
Myocardial Fibrosis ◽  
Fasting Blood Glucose ◽  
Left Ventricular ◽  
Heart Weight ◽  
Sensitivity Index ◽  
Protein Expressions ◽  
Diabetes Patients

To investigate the effect of Irbesartan on the changes of myocardial advanced glycation end products and their receptor (AGEs-RAGE), and matrix metalloproteinases (MMPs) systems in rat type 2 diabetes myocardial fibrosis model. All male Sprague-Dwaley rats were randomly divided into four groups: control (CON), high glucose and high-caloric diet (HC), type 2 diabetes (T2DM) and Irbesartan + T2DM (Ir+T2DM) groups. At 12th week, the fasting blood glucose (FBG) and fasting serum insulin (FINS) levels, insulin resistance index (IRI), insulin sensitivity index (ISI), body weight (BW), the ratio of heart weight/body weight (H/B), left ventricular weight index (LVWI), and cardiac col I, col III contents, plasma MMP-2, MMP-9 levels were evaluated. The protein expressions of col I, AGE, RAGE, MMP-2, MMP-14, and TIMP-2 were analyzed by Western blot. In the T2DM group, FBG, H/B, LVWI, IRI were increased ( P < 0.01), while FINS, BW, ISI were decreased in contrast to the CON and HC groups ( P < 0.05–0.01). In the Ir+ T2DM group, BW was higher, IRI, H/B, LVWI were lower than in the T2DM group. Compared with the CON and HC groups, the contents of col I and col III, the protein expressions of col I, AGE, RAGE, TIMP-2 and MMP-14 were increased, MMP-2 protein expression, the ratios of MMP-2/TIMP-2 and MMP-14/TIMP-2, MMP-2, and MMP-9 levels were decreased in the T2DM group ( P < 0.01). After Irbesartan treatment, all parameters were reversed. Irbesartan can ameliorate myocardial fibrosis in type 2 diabetes rat model, the likely mechanisms may be related to the down-regulation of AGEs-RAGE system and changes of MMPs pathway. Impact statement There are about 425 million diabetes patients (20–79 years) in the world according to the International Diabetes Federation Diabetes Atlas – 8th Edition. The cardiovascular complication is one of the major causes of death in diabetes patients. Myocardial fibrosis is one of the serious pathological changes, so investigating the pathogenesis of myocardial fibrosis has the significant value. Our study aims to investigate the effect of Irbesartan (the angiotensin II receptor antagonist) on the changes of AGE-RAGE system and MMP family components, and analyzes the potential mechanisms in type 2 diabetes-induced myocardial fibrosis. Our results provide the theoretical base for better understanding the pathogenesis in type 2 diabetes-induced myocardial complication. It is useful for clinicians to select the effective therapeutic measures for treatment of type 2 diabetes-induced organ fibrosis.

scholarly journals Histopathology from liver of tuvira ( Gymnotus spp.) parasitized by larvae of nematodes

2016 ◽  
Vol 46 (7) ◽  
pp. 1233-1239 ◽  
Author(s):  
Arlene Sobrinho Ventura ◽  
Márcia Mayumi Ishikawa ◽  
Andrea Maria de Araújo Gabriel ◽  
Helcy Lylian Nogueira Silbiger ◽  
Fabiana Cavichiolo ◽  
...  
Keyword(s):  
Histological Analysis ◽  
Parasitic Infection ◽  
Fish Farming ◽  
Health Condition ◽  
Histopathological Changes ◽  
Hepatic Parenchyma ◽  
Histological Changes ◽  
Balance Relation ◽  
Liver Changes ◽  
Hematoxylin Eosin

ABSTRACT: The aim of this study was to evaluate the histological changes in the liver of thirty-five Gymnotusspp. parasitized by endohelminths collected between April 2012 to October 2013 in commercial bait fish farming of Pantanal basin. Histological cuts of 7µm were stained with hematoxylin-eosin for parasites research and liver changes and have also been submitted to the Perls histochemical method for evaluation of hemosiderosis (Fe+++) based on the incidence degree and severity of change (Grade I, II and III) and tests for the presence of central melanomacrophages. Parasites identified were: Brevimulticaecumsp. with a prevalence of 22,9%, Eustrongylidessp 17,1%, Contracaecumtype I 68,7%, Contracaecumtype II 5,7%, Contracaecumtype III 5,7% and larvae of Anisakidae11,4%. Histological analysis showed intense disorganization of hepatic parenchyma with degenerate hepatocytes due to high parasitic infection, changes that can be deleterious and compromise the organism functioning, being harmful to the health of evaluated animals. Also evidencing normal tissue interleaved with different stages of Fe+++ deposit in grades II and III, injuring or destroing the cell. Histopathological changes in the tuvira's liver suggested a chronic response and the development of a balance relation between tuvira and parasitism by endohelminth identified in this study. There are also a testimony to the health condition of commercial bait fish farming on current ecosystem conditions.

scholarly journals A study on the histological changes resulting from certain virus infections of the potato

1932 ◽  
Vol 111 (769) ◽  
pp. 74-85 ◽  
Keyword(s):  
Virus Infection ◽  
Leaf Roll ◽  
The Other ◽  
Detailed Account ◽  
Virus Infections ◽  
Histopathological Changes ◽  
Histological Changes ◽  
Other Hand ◽  
Phloem Necrosis ◽  
Morbid Anatomy

A degeneration of the phloem elements of the potato, which occurs in plants suffering from Leaf-roll, was first described by Quanjer (1913). Since them phloem necrosis has been recorded by many workers, some ascribing it to specific pathogenic causes while others state that it occurs in both healthy and diseased plants. Esmarch (1921) states that it is always present in the ripening plant, and v. Brehmer (1923) shows three types of necrosis, viz., Nekrobiose , Obliteration and Necrosis , of which only the last is necessarily due to virus infection. On the other hand, Artschwager (1923) found necrotic changes only in virus infected and injured plants. In this paper an attempt is made to correlate the histopathological changes occuring in virus diseased plants with the external symptoms which they exhibit. A large part of the work is devoted to the morbid anatomy of certain “Streak” diseases, for a detailed account of the external symptons of which see Salaman and Bawden (1932).

Dendrobine attenuates isoniazid- and rifampicin-induced liver injury by inhibiting miR-295-5p

2020 ◽  
Vol 39 (12) ◽  
pp. 1671-1680
Author(s):  
R Ci ◽  
K Zhang ◽  
A Zhu ◽  
W Zang
Keyword(s):  
Oxidative Stress ◽  
Liver Injury ◽  
Luciferase Reporter ◽  
Protective Effects ◽  
Histological Changes ◽  
Stress Markers ◽  
Oxidative Stress Status ◽  
Protein Expressions ◽  
Polymerase Chain ◽  
Underlying Mechanisms

The present study aims to investigate the protective effects of Dendrobine and its underlying mechanisms on liver injury induced by isoniazid (INH) and rifampicin (RIF). A mouse model of liver injury was induced by intragastrically administration of 100 mg/kg INH and 100 mg/kg RIF for 14 days. The mice were intragastrically administrated with Dendrobine (50, 100, and 200 mg/kg) before the administration of INH and RIF. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. Oxidative stress markers including glutathione, superoxide dismutase, and malondialdehyde in the liver were measured and liver histopathological examinations were performed. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot were applied to determine the mRNA and protein expressions, respectively. Luciferase reporter assay was used to evaluate the interactions between miR-295-5p and CYP1A2. Dendrobine significantly decreased serum ALT and AST and inhibited the liver index and ameliorated the liver histological changes induced by INH and RIF. Besides, Dendrobine also regulated oxidative stress status in the liver by the regulation of CYP1A2. Moreover, mmu-miR-295-5p was identified to target CYP1A2 and to regulate the expression of CYP1A2. In summary, Dendrobine ameliorated INH and RIF induced mouse liver injury by miR-295-5p-mediated CYP1A2 expression.

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