Faculty Opinions recommendation of Ribosome profiling of mouse embryonic stem cells reveals the complexity and dynamics of mammalian proteomes.

2011 ◽  
Author(s):  
George Makhatadze ◽  
Dmitri Ermolenko
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Ribosome Profiling

scholarly journals Ribosome Profiling of Mouse Embryonic Stem Cells Reveals the Complexity and Dynamics of Mammalian Proteomes

Cell ◽  
2011 ◽  
Vol 147 (4) ◽  
pp. 789-802 ◽  
Author(s):  
Nicholas T. Ingolia ◽  
Liana F. Lareau ◽  
Jonathan S. Weissman
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Ribosome Profiling

scholarly journals A Chemical Kinetic Basis for Measuring Translation Initiation and Elongation Rates from Ribosome Profiling data

2018 ◽  
Author(s):  
Ajeet K. Sharma ◽  
Pietro Sormanni ◽  
Nabeel Ahmed ◽  
Prajwal Ciryam ◽  
Ulrike A. Friedrich ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Translation Initiation ◽  
Embryonic Stem ◽  
Chemical Kinetic ◽  
Mouse Embryonic Stem Cells ◽  
Ribosome Profiling ◽  
Next Generation Sequencing Data ◽  
Translation Rate ◽  
Large Variability

ABSTRACTAnalysis methods based on simulations and optimization have been previously developed to estimate relative translation rates from next-generation sequencing data. Translation involves molecules and chemical reactions; hence, bioinformatics methods consistent with the laws of chemistry and physics are more likely to produce accurate results. Here, we derive simple equations based on chemical kinetic principles to measure the translation-initiation rate, transcriptome-wide elongation rate, and individual codon translation rates from ribosome profiling experiments. Our methods reproduce the known rates from ribosome profiles generated from detailed simulations of translation. Applying our methods to data from S. cerevisiae and mouse embryonic stem cells we find that the extracted rates reproduce expected correlations with various molecular properties. A codon can exhibit up to 26-fold variability in its translation rate depending upon its context within a transcript. This broad distribution means that the average translation rate of a codon is not representative of the rate at which most instances of that codon are translated. We also find that mouse embryonic stem cells have a global translation speed of 5.2 AA/s, is similar to what has been previous reported using another analysis method. This large variability in translation rates suggests that translational regulation might be used by cells to a greater degree than previously thought.

Sign in / Sign up

Export Citation Format

Share Document