scholarly journals Ocorrência das infecções fúngicas subcutâneas em pacientes atendidos no laboratório de micologia médica do centro de pesquisa em medicina tropical / Occurrence of subcutaneous fungal infections in patients treated at the medical mycology laboratory of the tropical medicine research center

2021 ◽  
Vol 7 (12) ◽  
pp. 121016-121029
Author(s):  
Gabriella Borges Pereira ◽  
Cleudson Alan Ramos Lizardo ◽  
Laura Cristielen de Souza Carvalho ◽  
Elton Bill Amaral de Souza
Keyword(s):  
Fungal Infections ◽  
Tropical Medicine ◽  
Research Center ◽  
Medical Mycology ◽  
Medicine Research ◽  
Mycology Laboratory

scholarly journals Diagnosis of Breakthrough Fungal Infections in the Clinical Mycology Laboratory: An ECMM Consensus Statement

2020 ◽  
Vol 6 (4) ◽  
pp. 216 ◽  
Author(s):  
Jeffrey D. Jenks ◽  
Jean-Pierre Gangneux ◽  
Ilan S. Schwartz ◽  
Ana Alastruey-Izquierdo ◽  
Katrien Lagrou ◽  
...  
Keyword(s):  
Fungal Infections ◽  
Antifungal Susceptibility ◽  
Medical Mycology ◽  
Culture Techniques ◽  
Conventional Culture ◽  
Clinical Mycology ◽  
Diagnostic Modalities ◽  
Antigen Tests ◽  
Endemic Mycoses ◽  
The Impact

Breakthrough invasive fungal infections (bIFI) cause significant morbidity and mortality. Their diagnosis can be challenging due to reduced sensitivity to conventional culture techniques, serologic tests, and PCR-based assays in patients undergoing antifungal therapy, and their diagnosis can be delayed contributing to poor patient outcomes. In this review, we provide consensus recommendations on behalf of the European Confederation for Medical Mycology (ECMM) for the diagnosis of bIFI caused by invasive yeasts, molds, and endemic mycoses, to guide diagnostic efforts in patients receiving antifungals and support the design of future clinical trials in the field of clinical mycology. The cornerstone of lab-based diagnosis of breakthrough infections for yeast and endemic mycoses remain conventional culture, to accurately identify the causative pathogen and allow for antifungal susceptibility testing. The impact of non-culture-based methods are not well-studied for the definite diagnosis of breakthrough invasive yeast infections. Non-culture-based methods have an important role for the diagnosis of breakthrough invasive mold infections, in particular invasive aspergillosis, and a combination of testing involving conventional culture, antigen-based assays, and PCR-based assays should be considered. Multiple diagnostic modalities, including histopathology, culture, antibody, and/or antigen tests and occasionally PCR-based assays may be required to diagnose breakthrough endemic mycoses. A need exists for diagnostic tests that are effective, simple, cheap, and rapid to enable the diagnosis of bIFI in patients taking antifungals.

Medical Mycology: Laboratory Manual

Mycologia ◽  
1957 ◽  
Vol 49 (5) ◽  
pp. 776
Author(s):  
Libero Ajello ◽  
E. S. Beneke
Keyword(s):  
Medical Mycology ◽  
Laboratory Manual ◽  
Mycology Laboratory

scholarly journals History of the E.I. Martsinovsky Institute of Medical Parasitology and Tropical Medicine: research on malaria and leishmaniasis

2020 ◽  
Vol 27 (4) ◽  
pp. 1097-1124
Author(s):  
Margarita V. Strelkova ◽  
Alla M. Baranova ◽  
Katrin Kuhls
Keyword(s):  
Tropical Medicine ◽  
Medical Community ◽  
Medicine Research ◽  
History Of ◽  
Extensive Body ◽  
The Impact

Abstract This review presents the 100-year history of the Martsinovsky Institute of Medical Parasitology and Tropical Medicine in Moscow, Russia, starting with its foundation and early activities, and also describes the impact of its leading scientists, some of whom became internationally known. The institute headed a network of nine tropical institutes in the various Soviet republics from the 1920s to 1990. The extensive body of literature on the history and research accomplishments of this institute has mainly been published in Russian; our goal here is to introduce these achievements and this expertise to the international scientific and medical community, focusing on malaria and leishmaniasis and the development of measures to control and monitor these diseases in the USSR.

scholarly journals An alarming rise of non-albicans Candida species and uncommon yeasts in the clinical samples; a combination of various molecular techniques for identification of etiologic agents

2019 ◽  
Author(s):  
Mohireh Taei ◽  
Mostafa Chadeganipour ◽  
Rasoul Mohammadi
Keyword(s):  
Molecular Identification ◽  
Candida Species ◽  
Fungal Infections ◽  
Antifungal Susceptibility ◽  
Etiologic Agent ◽  
Molecular Techniques ◽  
University Hospital ◽  
Medical Mycology ◽  
Clinical Samples ◽  
Pcr Multiplex

Abstract Objective: Yeasts are opportunistic microorganisms can cause human fungal infection among immunocompromised patients. This study aimed to identify Candida species and uncommon yeasts obtained from clinical specimens in Kashani university hospital and Shefa Lab as a referral medical mycology laboratory, in Isfahan, Iran, by combination of various molecular techniques. Results: A total of 202 yeast strains were isolated from 341 clinical samples between February 2017 to May 2019. All clinical isolates were identified using phenotypic and molecular tests. PCR-RFLP, duplex-PCR, multiplex-PCR, and PCR-sequencing were applied for molecular identification of yeasts. The most clinical samples were obtained from urine (66.8%), nail (9.4%), bronchoalveolar lavage (5.9%), sore (4.4%), and blood (3.9%). One hundred and twenty-one Candida species were identified as non- albicans against 76 Candida albicans. Trichosporon asahii, and Pichia terricola were uncommon non- Candida yeasts isolated from urine samples. For the first time, we isolated P. terricola as etiologic agent of urinary tract infection in a pregnant female. Since non- albicans Candida species and non- Candida yeasts have various virulence factors and antifungal susceptibility profile, precise molecular identification can help us to reach to the advantageous strategies for treatment of these fungal infections.

scholarly journals SUSCEPTIBILITY TEST FOR FUNGI: CLINICAL AND LABORATORIAL CORRELATIONS IN MEDICAL MYCOLOGY

2015 ◽  
Vol 57 (suppl 19) ◽  
pp. 57-64 ◽  
Author(s):  
Ana ALASTRUEY-IZQUIERDO ◽  
Marcia S.C. MELHEM ◽  
Lucas X. BONFIETTI ◽  
Juan L. RODRIGUEZ-TUDELA
Keyword(s):  
Susceptibility Testing ◽  
Fungal Infections ◽  
Antifungal Susceptibility ◽  
Fungal Species ◽  
Medical Mycology ◽  
Candida Spp ◽  
Disease Epidemiology ◽  
Resistant Strains ◽  
European Standards

SUMMARYDuring recent decades, antifungal susceptibility testing has become standardized and nowadays has the same role of the antibacterial susceptibility testing in microbiology laboratories. American and European standards have been developed, as well as equivalent commercial systems which are more appropriate for clinical laboratories. The detection of resistant strains by means of these systems has allowed the study and understanding of the molecular basis and the mechanisms of resistance of fungal species to antifungal agents. In addition, many studies on the correlation of in vitro results with the outcome of patients have been performed, reaching the conclusion that infections caused by resistant strains have worse outcome than those caused by susceptible fungal isolates. These studies have allowed the development of interpretative breakpoints for Candida spp. and Aspergillus spp., the most frequent agents of fungal infections in the world. In summary, antifungal susceptibility tests have become essential tools to guide the treatment of fungal diseases, to know the local and global disease epidemiology, and to identify resistance to antifungals.

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