scholarly journals ANALISIS PEMBERIAN VITAMIN C PADA ROTIFER DAN ARTEMIA TERHADAP SINTASAN, RASIO RNA/DNA, KECEPATAN METAMORFOSIS DAN KETAHANAN STRES LARVA RAJUNGAN (Portunus Pelagicus) STADIA ZOEA

2020 ◽  
Vol 2 (2) ◽  
pp. 30-34
Author(s):  
Faidar Faidar ◽  
Sutia Budi ◽  
Erni Indrawati

Penelitian ini bertujuan untuk menganalisis kandungan vitamin C rotifer, artemia dan larva rajungan setelah diperkaya dengan vitamin C dan menganalisis pemberian vitamin C terhadap sintasan, rasio RNA/DNA, kecepatan metamorfosis dan ketahanan stres larva rajungan stadia zoea. Serta menentukan dosis optimum vitamin C yang menghasilkan sintasan, rasio RNA/DNA, ke-cepatan metamorfosis dan ketahanan stres larva rajungan stadia zoea yang terbaik. Penelitian ini dilaksanakan pada bulan Mei sampai Juni 2020 di unit pembenihan kepiting dan rajungan Balai Perikanan Budidaya Air Payau (BPBAP) Takalar. Analisis Rasio RNA/DNA dilakukan di Laboratorium Uji Fisika Kimia BPBAP Takalar. Rancangan penelitian menggunakan Rancangan Acak Lengkap (RAL). Hasil penelitian menunjukkan bahwa pemberian vitamin C pada rotifer dan artemia dapat meningkatkan kandungan rotifer, artemia dan larva rajungan. Pemberian vitamin C pada rotifer dan artemia terhadap larva rajungan stadia zoea dapat meningkatkan sintasan, kecepatan metamorfosis dan ketahanan stres larva rajungan dan Dosis vitamin C 250 ppm yang terbaik pada pemeliharaan larva rajungan stadia zoea. This study aimed to analyze the content of vitamin C rotifer, artemia and small crab larvae after enriched with vitamin C and to analyze the provision of vitamin C to survival, RNA / DNA ratio, metamorphosis rate and stress resistance of crab larvae in zoea stage. Also, it aimed to determine the optimum dose of vitamin C which produces the best survival rate, RNA/DNA ratio, metamorphosis speed and stress resistance of crab larvae in zoea stage. This research was conducted from May to June 2020 in the hatchery unit of the Brackish Water Cultivation Fishery Center (BPBAP) Takalar. RNA / DNA ratio analysis was carried out at the Laboratory of Chemical Physics Testing of BPBAP Takalar. The research design used a completely randomized design (CRD). The results showed that giving vitamin C to rotifers and brine shrimp increased the content of rotifers, brine shrimp and crab larvae. Giving vitamin C to rotifers and artemia to larvae of zoea stadia crabs can increase survival, metamorphosis speed and stress resistance of crab larvae and the best dose of vitamin C 250 ppm in the maintenance of zoea stadia crab larvae.

Author(s):  
Muthmainnah Muthmainnah ◽  
Muhammad Yusri Karim ◽  
Marlina Achmad

Blue swimming crab (Portunus pelagicus) is a sea crab that is widely found in Indonesian waters. One of the determinants of the success of crab cultivation is the availability of quality seeds. Howewer, blue swimming crab hatchery is currently experiencing problems, namely unstable seed availiability due to high mortality and growth at the larvae stage. This research aims to evaluate the effect of various colors of rearing basins of feeding rate and survival rate of blue swimming crab larvae (P. pelagicus) in zoea stage. The larvae for the study was zoea-1 stage with a stocking density of 50 ind/L. The basins used in this study was a round plastic basin with a volume of 40 L filled with 30 L of media water. The feed used was rotifer and artemia nauplius. The method used was a completely randomized design consist of 4 treatments and 3 replications. The treatments that were the use black, green, blue, and red basins colors. The results of the analysis of variance showed that the color of the basins had a significant effect on the feeding rate (p < 0,05) and very significant on survival rate (p < 0,01) for small blue swimming crab larvae were produced in black basins 90.14 and 16.60%.


2018 ◽  
Vol 5 (2) ◽  
pp. 249
Author(s):  
N. Putri Sumaryani ◽  
Anak Agung Istri Mirah Dharmadewi

This study was conducted to determine the temperature and storage time of the exact vitamin C content of red and white dragon fruit and the difference of vitamin C. The samples were taken from the plantation land in Ubud Andong Highway, Petulu, Gianyar Regency. Treated for 10 and 30 days with 2, 10, 20 and 300C temperature treatment with five repetitions using Completely Randomized Design Factorial pattern. Data obtained statistically using ANOVA (Analysis of Variance). The highest vitamin C content in red dragon fruit is 29.61 mg/ 100g temperature 100C for 10 days and the lowest is in 30 days storage with temperature 300C that is equal to 22,09 mg/ 100g. While the white dragon fruit content of vitamin C highest contained in storage with a temperature of 20C is 22.69 mg/ 100g with maximum storage of 10 days and the lowest at a temperature of 300C is 17.37 mg/ 100g.


2018 ◽  
Vol 3 (4) ◽  
pp. 808-821
Author(s):  
Cut Betari Arhandhi ◽  
Yuliani Aisyah ◽  
Rasdiansyah Rasdiansyah

Abstrak. Umbi bit merupakan salah satu bahan pangan yang dapat digunakan sebagai pewarna alami pada pembuatan soft candy seperti rshmallow. Penelitian ini dilakukan dengan menggunakan Rancangan Acak Lengkap (RAL) dengan pola faktorial yang terdiri atas dua faktor. Faktor pertama yaitu konsentrasi ekstrak umbi bit (B) yang terdiri dari 3 taraf yaitu B1 = 5% B2 = 10% dan B3 = 15%. Faktor kedua adalah konsentrasi gelatin (G) yang terdiri dari 3 taraf yaitu G1 = 3% G2 = 5% dan G3 = 8%. Ulangan dilakukan sebanyak 3 kali sehingga diperoleh 27 satuan percobaan. Hasil penelitian menunjukkan bawa konsentrasi ekstrak umbi bit berpengaruh sangat nyata terhadap kadar air, vitamin C dan aktivitas antioksidan serta uji deskrpsi aroma, rasa, warna buram, tekstur, dan mouthfeel marshmallow umbi bit. Adapun konsentasi gelatin berengaruh nyata terhadap kadar vitamin C dan uji deskripsi tekstur marshmallow umbi bit. Marshmallow umbi bit memiliki kadar air yaitu sebesar 22,06%, kadar vitmin C 80,67 Mg dan aktivitas antioksidan sebesar 30%.Abstract: Beetroot is one of food that can be used as natural dyes in making of soft candy like marshmallows. This research was conducted using a Completely Randomized Design (CRD) with a factorial pattern consisting of two factors. The first factor is the concentration of beet tuber extract (B) which consists of 3 levels, namely B1 = 5% B2 = 10% and B3 = 15%. The second factor is the concentration of gelatin (G) which consists of 3 levels, namely G1 = 3% G2 = 5% and G3 = 8%. Tests were carried out 3 replications so that 27 trial units were obtained. The results showed that the concentration of coffee extract had a very significant effect on water content, vitamin C and antioxidant activity, as well as the description of aroma, flavor, opaque color, texture, and mouthfeel marshmallow tuber content. The concentration of gelatin significantly affected vitamin C levels and test description of marshmallow tuber tuber texture. Marshmallow beetroot has a water content of 22.06%, vitamin C content of 80.67 Mg and antioxidant activity of 30%.


2017 ◽  
Vol 6 (1) ◽  
pp. 14
Author(s):  
Rachel Breemer ◽  
Priscillia Picauly ◽  
Nurhayati Hasan

This research was aimed to determine the exact glycerol concentration in the making of edible coating to coat the tomatoes so that the shelf life is extended and the quality is maintained. A Completely Randomized Design with one factor (RAL): glycerol concentration (without coating, 10%, 30%, 50%) was applied. The observed variables were weight shrinkage, color, hardness, total acid and vitamin C. Results showed that for 10 days storage period, the best response of the variables observed was obtained by tomatoes treated with edible coating 10% glycerol concentration that can maintain the quality of tomato and economically feasible than glycerol concentration of 30% and 50%.


Author(s):  
Azaria Dhea Gantina ◽  
Farida Fathul ◽  
Liman Liman ◽  
Muhtarudin Muhtarudin

This research aims was to determine the effect of bokashi dose on plant height, number of tillers, percentage of stems, fresh production, and dry matter production and also to determine the optimum dose of bokashi to obtain the best results on growth and production at the first harvest of dwarf elephant grass. This research was conducted in March 2020 - July 2020 in Sidodadi Asri village, Jati Agung district, South Lampung regency. This research used a Completely Randomized Design (CRD) with 4 levels of treatment, namely P0 (control); P1 (bokashi dosage 30 tons/ha); P2 (bokashi dosage 40 tons/ha); and P3 (bokashi dosage 50 tons/ha). Each experimental treatment unit has plot measuring 2x2 m2. Each trial unit was repeated 3 times, so there were 12 trial units. The data were analyzed by variations analyzed at 5% and or 1% level, after that it was followed by orthogonal polynomial test. The results showed that the dose of bokashi had a significant effect (P<0,05) on plant height, fresh production, and dry production, but had no significant effect (P>0,05) on number of tillers, and percentage of stems. The effect of bokashi fertilizer on plant height was obtained by using the optimum bokashi dose of 36.73 tons/ha and the equation was = 60,13 + 35,38x - 6.62x2 [0 ; 50 ton/Ha] (P<0,01). The optimum dose was not obtained for the number of tillers, percentage of stems, fresh production, and dry production. Keywords: Plant height, Number of tillers, Percentage of stems, Fresh production, Dry production


2021 ◽  
Vol 1 (1) ◽  
pp. 6-11
Author(s):  
Sefti Heza Dwinanti ◽  
Ratu Brata T. Mengkrin ◽  
Ade Dwi Sasanti

Starfruit contains active ingredients such as flavonoids, phenols, antioxidants and vitamin C so that it can be used as an immunostimulant in catfish farming activities. Motile Aeromonas Septicemia (MAS) is one of the obstacles that can cause catfish farming losses. This study aims to determine the effect of differences in timing of feeding containing starfruit juice to protect catfish from MAS disease. The study was designed using a completely randomized design (CRD) consisting of 6 treatments (P) in the form of feeding duration containing starfruit for 7 days (P1), 14 days (P2), 21 days (P3), 28 days (P4). and 35 days (P5) and control of feeding without star fruit juice (P0). The research was carried out at the Aquaculture Laboratory, Sriwijya University. The dose of star fruit juice mixed in the feed is 300 mL.Kg-1 while the catfish used is 8-12 cm in size. The challenge test used Aeromonas hydrophilla bacteria with a density of 108 CFU.mL-1 which was injected as much as 0.5 mL.tail-1 intramuscularly. The results showed that administration containing starfruit juice for 28 days provided the best protection. Based on the hematological test the day before and before infection, the delta decreased the hematocrit by 3% and the delta decreased the erythrocyte count by 3,103 cells.mL-1. In addition, live feed after infection was 100%, prevalence was 17.78% and there was no effect of giving starfruit juice on growth and feed efficiency.


2021 ◽  
Vol 10 (1) ◽  
pp. 108
Author(s):  
I Wayan Ryantama Swastika Braja ◽  
I Dewa Gede Mayun Permana ◽  
I Ketut Suter

Loloh teter leaf is one of the traditional drinks in Bali, it made from raw material of teter leaf and other matter such as tamarind fruit, sugar and salt. The study aims to determine the effect of additional tamarind fruit (Tamarindus indica L.) to produce Loloh Teter leaf (Solanum erianthum) with the best characteristics. The experimental design used was a Completely Randomized Design (CRD) with the addition of tamarind fruit treatment consisting of 6 levels, namely : 0%, 0.5%, 1%, 1.5%, 2% and 2.5%. The treatment was repaeated 3 times for abtaining obtained 18 experimental units. The data obtained were analyzed by variance and if the treatment had significant effect followed by Duncan Multiple Range Test (DMRT). The results of this study indicate that the treatment of tamarind fruit addition has a very significant effect (P<0,01) on total phenolics, total flavonoids, vitamin C, antioxidant activity, total solids, color (hedonic test) and taste (scoring test). The best treatment in this study was the addition of 2,5% tamarind fruit with the total phenolics 0.86 GAE/100ml, total flavonoids 0.48 QE/100ml, vitamin C 26.39 mg/100ml, antioxidant activity 47.05% with IC50 valued 271,382.73 ppm, total solids 7.44%, the color is usual as well as, the aroma is rather liked, the teste is sour and rather liked and overall acceptance is rather liked.


2018 ◽  
Vol 1 (2) ◽  
pp. 162-171 ◽  
Author(s):  
Carly Purba ◽  
Hotnida Sinaga ◽  
Mimi Nurminah

The purpose of this research was to determine the effect of ratio of moringa leaves juice with pineapple juice and the best percentage of arabic gum on the quality of jelly candy. This research was using completely randomized design with two factors, i.e. : ratio of moringa leaves juice with pineapple juice (K): (100%:0% ; 75%:25% ; 50%:50% ; 25%:75% ; 0%:100%) and arabic gum percentage (G): (1,0% ; 1,5% ; 2,0%). Parameters analyzed were moisture content, ash content, vitamin C content, total soluble solid, total acid, lightness value (L*), hedonic organoleptic values of colour, flavour, taste, texture and score organoleptic values of colour and texture. The results showed that the ratio of of moringa leaves juice with pineapple juice had highly significant effect on ash content, vitamin C content, total acid, lightness value, hedonic organoleptic test of colour, flavour, taste and score organoleptic value of colour. The ratio of  moringa leaves juice with pineapple juice (25%:75%) and 2,0% arabic gum percentage produces the best quality of jelly candy based on hedonic organoleptic values of colour, flavour, taste, texture and score organoleptic values of colour and texture.


2020 ◽  
Vol 3 (1) ◽  
pp. 9
Author(s):  
Yusra Yusra ◽  
Asnani Asnani ◽  
Sri Rejeki

ABSTRACT         This research aims to study the effect of mackerel surimi substitution and the chemical composition of the nuggets. This study uses a Completely Randomized Design (CRD) consisting of four treatments namely P1 (control) (100% crab meat: 0% surimi mackerel), P2 (70% crab meat: 30% mackerel surimi), P2 (crab meat 60%: mackerel surimi 60%) and P4 (50% crab meat: 50% mackerel surimi). The results of crab nugget research substituted with mackerel surimi obtained the best treatment organoleptic test obtained at P2 treatment with a fairly good panelist assessment. The best organoleptic test was found in the P2 treatment with the values of the color, aroma, texture, and taste parameters respectively 7.2, 6.2, 7.2 and 7.2. The highest crab fish substitution surimi crab nugget crab test on the P4 treatment with the value of water content, protein content and fat content was 45.25%. 29.79% and 4.61% respectively. Keywords: small crab, mackerel, nuggets, surimi, organoleptic test.ABSTRAKPenelitian ini bertujuan untuk mempelajari pengaruh substitusi surimi ikan kembung dan komposisi kimia nugget. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari empat perlakuan yaitu P1 (kontrol) (100% daging rajungan : 0% surimi ikan kembung), P2 (daging rajungan 70% : surimi ikan kembung 30%), P2 (daging rajungan 60% : surimi ikan kembung  60%) dan P4 (daging rajungan 50% : surimi ikan kembung 50%). Hasil penelitian nugget rajungan yang disubstitusikan dengan surimi ikan kembung diperoleh perlakuan terbaik uji organoleptik diperoleh pada perlakuan P2 dengan penilaian panelis yang cukup baik. Rerata uji organoleptik terbaik terdapat pada perlakuan P2 dengan nilai parameter warna, aroma, tekstur, dan rasa berturut-turut adalah 7,2, 6,2, 7,2 dan 7,2. Uji kimia nugget rajungan substitusi surimi ikan kembung tertinggi pada perlakuan P4 dengan nilai kadar air, kadar protein dan kadar lemak berturut-turut 45,25%. 29,79% dan 4,61%.Kata kunci: rajungan, ikan kembung, nugget, surimi, uji organoleptik.


2021 ◽  
Vol 10 (1) ◽  
pp. 64-77
Author(s):  
Eries D Mustikarini ◽  
G Ibnu Prayoga ◽  
R Santi ◽  
Siti Khodijah ◽  
T Lestari

Dryland area in Indonesia reaches 108.8 millions ha (69.4%) of the total dry land with the potential to expand the area of food crops reaching 7.1 million ha, which is dominated by ultisol and oxisol soil types. Plant constraint cultivation on ultisol soil types is the acidic soil pH with high Al and Fe levels. One of the efforts that can be made to cultivate plants on ultisol soil is the selection of plants that are tolerant of Fe stress. This study aimed to determine a tolerance level and F6 red rice lines that were tolerant of iron (Fe) stress in ultisol soils. The experimental design used was factorial Completely Randomized Design (CRD) consisting of 2 factors, the first factor consisted of 12 treatments (10 test lines and 2 comparison varieties) and the second factor  was iron stress with concentrations (0 ppm and 551,55 ppm). The stress was applied after 3 WAP. The stress resistance test to iron (Fe) should that all lines of red rice F6 on ultisol soils have a score of 1 which indicates the stress tolerance of Fe at a concentration of 551,55 ppm Fe. The 23A-56-30-25-12 and 23A-56-30-25-13 lines were the best lines that were tolerant of 551,55 ppm iron (Fe) stress with the highest growth and yield. The lines tested can be developed on ultisol soils because they are tolerant of Fe.


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