Serum and non-serum method-induced differentiation of mouse embryonic stem cells into definitive endoderm

2013 ◽  
Vol 33 (2) ◽  
pp. 124-129
Author(s):  
Yang-fang LI ◽  
Yong LAN ◽  
Ya-zhuo ZHANG ◽  
Xin WANG ◽  
Yi-ping HU
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Definitive Endoderm ◽  
Induced Differentiation

scholarly journals Erythropoietin facilitates definitive endodermal differentiation of mouse embryonic stem cells via activation of ERK signaling

2017 ◽  
Vol 312 (5) ◽  
pp. C573-C582 ◽  
Author(s):  
Taku Kaitsuka ◽  
Kohei Kobayashi ◽  
Wakako Otsuka ◽  
Takuya Kubo ◽  
Farzana Hakim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Pluripotent Stem Cells ◽  
Early Stage ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Definitive Endoderm ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Pancreatic Differentiation

Artificially generated pancreatic β-cells from pluripotent stem cells are expected for cell replacement therapy for type 1 diabetes. Several strategies are adopted to direct pluripotent stem cells toward pancreatic differentiation. However, a standard differentiation method for clinical application has not been established. It is important to develop more effective and safer methods for generating pancreatic β-cells without toxic or mutagenic chemicals. In the present study, we screened several endogenous factors involved in organ development to identify the factor, which induced the efficiency of pancreatic differentiation and found that treatment with erythropoietin (EPO) facilitated the differentiation of mouse embryonic stem cells (ESCs) into definitive endoderm. At an early stage of differentiation, EPO treatment significantly increased Sox17 gene expression, as a marker of the definitive endoderm. Contrary to the canonical function of EPO, it did not affect the levels of phosphorylated JAK2 and STAT5, but stimulated the phosphorylation of ERK1/2 and Akt. The MEK inhibitor U0126 significantly inhibited EPO-induced Sox17 expression. The differentiation of ESCs into definitive endoderm is an important step for the differentiation into pancreatic and other endodermal lineages. This study suggests a possible role of EPO in embryonic endodermal development and a new agent for directing the differentiation into endodermal lineages like pancreatic β-cells.

scholarly journals Hypoxia Enhances Differentiation of Mouse Embryonic Stem Cells into Definitive Endoderm and Distal Lung Cells

2015 ◽  
Vol 24 (5) ◽  
pp. 663-676 ◽  
Author(s):  
Pimchanok Pimton ◽  
Shimon Lecht ◽  
Collin T. Stabler ◽  
Gregg Johannes ◽  
Edward S. Schulman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Definitive Endoderm ◽  
Lung Cells ◽  
Distal Lung
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