Genetic diversity and correlation analysis of starch yield-related traits in sweet potato

A batata-doce (Ipomoea batatas (L.) Lam.) tem sido reportada como uma das espécies de planta com grande capacidade de converter biomassa em matéria prima para produção de etanol. O objetivo do trabalho foi avaliar o potencial de clones de batata-doce para produção de etanol. Foram avaliados 60 clones de batata-doce para produtividade de raízes, teor de amido nas raízes, produtividade de amido, coloração da casca e da polpa e o rendimento de etanol. O clone BDTO#122,32 e as cultivares Ana Clara e Carolina Vitória com média de 46,77; 42,75 e 41,25 t ha-¹, respectivamente, foram os que mais conseguiram acumular biomassa na forma de raiz. Os clones que apresentam as maiores médias de produtividade de amido por hectare foram BDTO#144.22 e BDTO#100.23, com valores de 15,46 e 14,16% t ha-1, com rendimentos de etanol de 8,33 e 7,63 m³ ha-¹. Os clones BDTO#144.22 e BDTO#100.23 apresentaram as maiores médias de produtividade de amido por hectare e rendimento de etanol, sendo, portanto, os mais promissores para a produção de etanol.Palavras-chave: Ipomoea batatas (L.) Lam, melhoramento genético, seleção, biocombustível. POTENTIAL OF EXPERIMENTAL CLONES OF SWEET POTATO FOR ETHANOL PRODUCTION ABSTRACT:Sweet potato (Ipomoea batatas (L.) Lam.) Has been reported as one of the plant species with great ability to convert biomass into feedstock for ethanol production. The objective of this work was to evaluate the potential of sweet potato clones for ethanol production. Twenty-six sweet potato clones were evaluated for root productivity, root starch content, starch yield, bark and pulp color, and ethanol yield. Clone BDTO # 122.32 and cultivars Ana Clara and Carolina Vitória averaging 46.77; 42.75 and 41.25 t ha-1, respectively, were the ones that were able to accumulate biomass in the root form. The clones presenting the highest starch productivity per hectare were BDTO # 144.22 and BDTO # 100.23, with values of 15.46 and 14.16% t ha-1, with ethanol yields of 8.33 and 7.63 m³ ha-¹. The clones BDTO # 144.22 and BDTO # 100.23 showed the highest averages of starch productivity per hectare and yield of ethanol, thus being the most promising for the production of ethanol.Keywords: Ipomoea potatoes (L.) Lam, breeding, selection, biofuel.

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Genetic Diversity of Sweet potato feathery mottle virus from Sweet Potatoes in Korea

The Plant Pathology Journal ◽

10.5423/ppj.2007.23.1.013 ◽

2007 ◽

Vol 23 (1) ◽

pp. 13-21 ◽

Cited By ~ 3

Author(s):

Hae-Ryun Kwak ◽

Mi-Kyeong Kim ◽

Mi-Nam Jung ◽

Su-Heon Lee ◽

Jin-Woo Park ◽

...

Keyword(s):

Genetic Diversity ◽

Sweet Potato ◽

Mottle Virus ◽

Sweet Potatoes

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GENETIC DIVERSITY AMONG SWEET POTATO CROPS CULTIVATED BY TRADITIONAL FARMERS

Revista Caatinga ◽

10.1590/1983-21252018v31n329rc ◽

2018 ◽

Vol 31 (3) ◽

pp. 779-790 ◽

Cited By ~ 1

Author(s):

PABLO FORLAN VARGAS ◽

ERIC WATZKE ENGELKING ◽

LUIS CARLOS FERREIRA DE ALMEIDA ◽

ELIEL ALVES FERREIRA ◽

HAMILTON CESAR DE OLIVERIA CHARLO

Keyword(s):

Genetic Diversity ◽

Sweet Potato ◽

Ipomoea Batatas ◽

Block Design ◽

Genetic Material ◽

Morphological Characteristics ◽

Leaf Size ◽

Stem Length ◽

Phenotypic Correlation ◽

Crop Species

ABSTRACT The genetic variability within the crop species Ipomoea batatas is broad, hence, in order to support future breeding programs it is of the utmost importance that germplasm banks be created, conserved, and characterized. Therefore, the objective of this work was to rescue and evaluate the genetic divergence in sweet potato accessions collected in traditional communities of Vale do Ribeira Paulista. Sweet potato samples were collected from quilombos, indigenous villages, caiçaras communities, and small farms. The study was conducted between February 2013 and August 2014 in a randomized block design with three replications. Genetic material included 95 collected accessions and two commercial cultivars. Morphological characteristics of the accessions were evaluated and distances in the genetic distance matrix were estimated by means of multi-category variables, the data being subsequently clustered by the Tocher method. Analysis of the relative contribution of each characteristic and phenotypic correlation of descriptors was also performed. Results evidenced wide genetic diversity among the sweet potato accessions collected in Vale do Ribeira, which were not grouped according to the collection point. The descriptors that contributed more than 60% of genetic diversity included: leaf size, general leaf profile, immature leaf color, petiole pigmentation, predominant branch color, branch secondary color, stem length, cortical thickness, predominant periderm color, and periderm color intensity. Correlations between morphological descriptors was observed in 22.26% of the paired traits.

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Genetic diversity in Brazilian sweet potato (Ipomoea batatas (L.) Lam., Solanales, Convolvulaceae) landraces assessed with microsatellite markers

Genetics and Molecular Biology ◽

10.1590/s1415-47572008000400020 ◽

2008 ◽

Vol 31 (3) ◽

pp. 725-733 ◽

Cited By ~ 35

Author(s):

Elizabeth Ann Veasey ◽

Aline Borges ◽

Mariana Silva Rosa ◽

Jurema R. Queiroz-Silva ◽

Eduardo de Andrade Bressan ◽

...

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Sweet Potato ◽

Ipomoea Batatas

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Application of SSR Markers in Determination of Putative Resistance to SPVD and Genetic Diversity among Orange Fleshed Sweet Potato

Journal of Advances in Biology & Biotechnology ◽

10.9734/jabb/2016/27582 ◽

2016 ◽

Vol 9 (2) ◽

pp. 1-10 ◽

Cited By ~ 1

Author(s):

S Kiarie ◽

L Karanja ◽

M Obonyo ◽

F Wachira

Keyword(s):

Genetic Diversity ◽

Sweet Potato ◽

Ssr Markers ◽

Orange Fleshed Sweet Potato

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First Report of Sweet potato leaf curl Georgia virus on Sweet Potato in China

Plant Disease ◽

10.1094/pdis-10-12-0967-pdn ◽

2013 ◽

Vol 97 (10) ◽

pp. 1388-1388 ◽

Cited By ~ 2

Author(s):

Y. Qin ◽

Z. Zhang ◽

Z. Qiao ◽

Q. Qiao ◽

D. Zhang ◽

...

Keyword(s):

Genetic Diversity ◽

Nucleotide Sequence ◽

Sweet Potato ◽

Leaf Growth ◽

Negative Control ◽

Post Inoculation ◽

Natural Occurrence ◽

Potato Leaf ◽

First Report ◽

Leaf Curl

Begomoviruses infecting sweet potato (Ipomoea batatas) are phylogenetically distinct from other members of the genus Begomovirus, and have been named “sweepoviruses” (1). Sweepoviruses cause sweet potato yield losses and cultivar decline, and have been found in China (1,3). In 2011, a survey was conducted to determine the incidence, genetic diversity, and distribution of sweepoviruses in China. Thirty sweet potato cuttings showing upward leaf curl, leaf roll, chlorosis, and stunting were collected from fields in Jiangsu, Guangxi, Guizhou, Shanxi, Henan, and Hebei Provinces. Five-leaf growth stage I. setosa plants were inoculated by side-grafting with scions from these samples, and grown in an insect-proof greenhouse in 20-cm-diameter clay pots. Each sample was grafted onto three replicate plants. Healthy, non-grafted I. setosa plants were used as the negative control treatment. Total nucleic acids were extracted from 100 mg fresh leaves harvested 30 days post-inoculation (dpi) from symptomatic and negative control plants using the Universal Genomic DNA Extraction Kit (TaKaRa, Dalian, China). Universal primers for amplification of Geminiviruses (BM-V [5′-KSGGGTCGACGTCATCAATGACGTTRTAC-3′] and BM-C [5′-AARGAATTCATKGGGGCCCARARRGACTGGC-3′]) (2) were used to amplify the begomovirus A component by PCR assay. A DNA fragment of the expected size (2.8 kb) was obtained from grafted leaf samples of the Hebei Province plant, and was cloned into the pMD-19T vector (TaKaRa). The recombinant plasmid was transformed into competent cells of Escherichia coli strain JM109, and the inserted fragment sequenced. The nucleotide sequence obtained (GenBank Accession No. JX448368) was 2,785 nt long, and contained two open reading frames (ORFs) in the virion sense, and four ORFs in the complementary sense, similar to other monopartite begomoviruses (1). The sequence was compared with sequences in GenBank using BLAST. The results revealed the greatest nucleotide sequence identity, 90.8%, with that of the Sweet potato leaf curl Georgia virus (SPLCGV) from Georgia, United States (AF326775). The sequence also shared identities of <89% with other sweepoviruses, and was therefore designated SPLCGV-China: Hebei: 2011. Comparison of the complete genome sequence of SPLCGV-China: Hebei: 2011 with SPLCGV revealed an 18 nucleotide insertion between AV-1 and AC-3. The results confirmed that the sweet potato sample from which SPLCGV-China: Hebei: 2011 was obtained was infected with SPLCGV. To our knowledge, this is the first report of the natural occurrence of SPLCGV in China. This study will assist with understanding the presence of this virus and genetic diversity of sweepoviruses in China. References: (1) H. P. Bi and P. Zhang. Arch. Virol. 157:441, 2012. (2) R. W. Briddon and P. G. Markham. Mol. Biotechnol. 1:202, 1994. (3) Y. S. Luan et al. Virus Genes 35:379, 2007.

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Genetic diversity analysis of sweet potato [Ipomoea batatas (L.) Lam.] germplasms through RAPD and ISSR markers

Bangladesh Journal of Botany ◽

10.3329/bjb.v50i1.52679 ◽

2021 ◽

Vol 50 (1) ◽

pp. 119-129

Author(s):

PN Narasimha Murthy ◽

NB Patel ◽

Chintan Kapadia ◽

KD Desai ◽

G Koteswara Rao

Keyword(s):

Genetic Diversity ◽

Sweet Potato ◽

Ipomoea Batatas ◽

Genetic Relatedness ◽

Issr Markers ◽

Diversity Analysis ◽

Genetic Diversity Analysis ◽

Polymorphic Loci ◽

Improvement Programme ◽

Rapd And Issr

Sweet potato [Ipomoea batatas (L.) Lam.] germplasms are unique as they are heterozygous, vegitatively as well as sexually propagated and polyploid in nature. Forty-four germplasms from local farmer’s field and from various centres of India were determined using 15 RAPD and 15 ISSR markers to evaluate their genetic relatedness. Average polymorphisms for RAPD and ISSR markers were found to be 86.72 and 81.64%, respectively. The OPM series primer produced 145 total bands with 138 polymorphic loci and ISSR markers gave 116 total bands with 101 polymorphic loci. The clusters were made using the UPGMA method using both the RAPD and ISSR data and group diverse yet similar germplasm in two different clusters. This gives ample information to use them in further improvement programme.

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