Interactions of Acidithiobacillus ferrooxidans with Heavy Metals, Various Forms of Arsenic and Pyrite

2007 ◽  
Vol 20-21 ◽  
pp. 423-426 ◽  
Author(s):  
Shailesh R. Dave ◽  
K.H. Gupta
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Ferrous Iron ◽  
Ferrous Sulphate ◽  
Pyrite Oxidation ◽  
Environmental Parameters ◽  
Total Iron ◽  
Oxidation Study ◽  
Planktonic Bacteria ◽  
Presence And Absence ◽  
Ks Values

An arsenic resistant ferrous iron oxidizing bacterium Acidithiobacillus ferrooxidans (GenBank no. EF010878) was isolated from reactor leachate. The reactor leachate showed extreme environmental parameters. Ferrous iron concentrations of more than 60 g/L were found to be inhibitory in the presence and absence of arsenite. Ks values of 12.5 and 8.0 g/L ferrous sulphate and Vmax of 0.124 and 0.117 g/L/h/0.8 mg of protein were found in the presence and absence of arsenite respectively. At 14.9 g/L of arsenite and arsenate the culture showed 26.8 and 59.7 % ferrous iron oxidizing activity respectively. Amongst the metals studied, copper was found to be more toxic as compared to nickel and zinc. In the presence of 3.51 g/L nickel or 4.68 g/L zinc, about 30 % biooxidation activity was registered. In the pyrite oxidation study 87, 67 and 64 % of pyrite oxidation was found and 2.02, 3.19 and 5.96 g/L total iron was solubilized with 5, 10 and 20 g/L of pyrite respectively. The isolate was also able to oxidize refractory arsenopyrite gold ore and 0.531 g/L of arsenic was solubilized along with 0.872 g/L of soluble total iron. During this period the numbers of planktonic bacteria increased from 2.4 x 106 to 1.0 x 108 cells/mL.

Investigation of intermediate sulfur species during pyrite oxidation in the presence and absence of Acidithiobacillus ferrooxidans

2017 ◽  
Vol 167 ◽  
pp. 58-65 ◽  
Author(s):  
Zhihong Tu ◽  
Chuling Guo ◽  
Ting Zhang ◽  
Guining Lu ◽  
Jingjing Wan ◽  
...  
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Pyrite Oxidation ◽  
Sulfur Species ◽  
Presence And Absence

Biofilm Formation, Communication and Interactions of Mesophilic Leaching Bacteria during Pyrite Oxidation

2013 ◽  
Vol 825 ◽  
pp. 107-110
Author(s):  
Sören Bellenberg ◽  
Robert Barthen ◽  
Mario Vera ◽  
Nicolas Guiliani ◽  
Wolfgang Sand
Keyword(s):  
Quorum Sensing ◽  
Acidithiobacillus Ferrooxidans ◽  
Biofilm Formation ◽  
Pyrite Oxidation ◽  
Cell Communication ◽  
Homoserine Lactone ◽  
Mixed Cultures ◽  
Acyl Homoserine Lactone ◽  
Leaching Bacteria ◽  
Leaching Efficiency

A functional luxIR-type Quorum Sensing (QS) system is present in Acidithiobacillus ferrooxidans. However, cell-cell communication among various acidophilic chemolithoautotrophs growing on pyrite has not been studied in detail. These aspects are the scope of this study with emphasis on the effects exerted by the N-acyl-homoserine lactone (AHL) type signaling molecules which are produced by Acidithiobacillus ferrooxidans. Their effects on attachment and leaching efficiency by other leaching bacteria, such as Acidithiobacillus ferrivorans, Acidiferrobacter spp. SPIII/3 and Leptospirillum ferrooxidans in pure and mixed cultures growing on pyrite is shown.

Reusability of Fenton Sludge to Reduce COD and Color on Palm Oil Mill Secondary Effluent (POMSE)

2015 ◽  
Vol 1113 ◽  
pp. 486-491 ◽  
Author(s):  
Nurul Shairah Ahmad Shahrifun ◽  
Norul Nazilah Ab’lah ◽  
Hazilia Hussain ◽  
Azmi Aris ◽  
Qistina Omar ◽  
...  
Keyword(s):  
Palm Oil ◽  
Ferrous Iron ◽  
Total Iron ◽  
Fenton Oxidation ◽  
Color Removal ◽  
Secondary Effluent ◽  
Percentage Removal ◽  
Palm Oil Mill

This paper reports on the reusability of iron sludge generated from the solar Fenton oxidation on Palm Oil Mill Secondary Effluent (POMSE) samples. The aim of this study was to observe the reusability of Fenton sludge to be used as iron sources for reducing the COD and color of POMSE. The feasibility of iron sludge was determined by recycling the iron sludge five times. Result showed that the percentage removal of COD and color was generally reduced from fresh iron used (F1) to Recycled 1 (R1) and eventually Recycle 5 (R5). The percentage removal of COD for F1, R1, R2, R3, R4, and R5 were 83.8%, 71.2%, 40.3%, 45.1%, 39% and 35.9% respectively. Meanwhile the color removal for F1-R5 were 97.18%, 87.29%, 75.33%, 73.08%, 68.60% and 70.84% respectively. The amount of ferrous iron and total iron in supernatant however, fluctuated between 0.00 to 0.37 and 2.86 to 9.24mg/L respectively. Amount of iron that remained in the sludge in every cycle starting from F1 to R5 were 7271, 7404, 7275, 7546, 7238 and 7025mg iron/kg sludge respectively. In conclusion, it was justifiable to recycle Fenton sludge as a high amount of iron remained even after the fifth time of recycling.

scholarly journals A kinetic model of ferrous iron oxidation by Acidithiobacillus ferrooxidans in a batch culture

2005 ◽  
Vol 11 (2) ◽  
pp. 59-62 ◽  
Author(s):  
Dragisa Savic ◽  
Miodrag Lazic ◽  
Vlada Veljkovic ◽  
Miroslav Vrvic
Keyword(s):  
Kinetic Model ◽  
Acidithiobacillus Ferrooxidans ◽  
Ferrous Iron ◽  
Bubble Column ◽  
Iron Oxidation ◽  
Yield Coefficient ◽  
Transfer Rates ◽  
Ferrous Iron Oxidation ◽  
Menten Kinetic ◽  
Kinetics Of

The batch oxidation kinetics of ferrous iron by Acidithiobacillus ferrooxidans were examined at different oxygen transfer rates and pH in an aerated stirred tank and a bubble column. The microbial growth, oxygen consumption rate and ferrous and ferric iron were monitored during the biooxidation. A kinetic model was established on the basis of the Michaelis-Menten kinetic equation for bacterial growth and the constants estimated from experimental data (maximum specific growth rate 0.069 h-1, saturation constant 2.9 g/dm3, and biomass yield coefficient based on ferrous iron 0.003 gd.w./gFe). Values calculated from the model agreed well with the experimental ones regardless of the bioreactor type and pH conditions.

scholarly journals The High-Molecular-Weight Cytochrome c Cyc2 of Acidithiobacillus ferrooxidans Is an Outer Membrane Protein

2002 ◽  
Vol 184 (1) ◽  
pp. 313-317 ◽  
Author(s):  
Andrés Yarzábal ◽  
Gaël Brasseur ◽  
Jeanine Ratouchniak ◽  
Karen Lund ◽  
Danielle Lemesle-Meunier ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Outer Membrane ◽  
High Molecular Weight ◽  
Acidithiobacillus Ferrooxidans ◽  
Ferrous Iron ◽  
Membrane Fraction ◽  
Cytoplasmic Membrane ◽  
Respiratory Pathway ◽  
Membrane Components ◽  
Membrane Level

ABSTRACT A high-molecular-weight c-type cytochrome, Cyc2, and a putative 22-kDa c-type cytochrome were detected in the membrane fraction released during spheroplast formation from Acidithiobacillus ferrooxidans. This fraction was enriched in outer membrane components and devoid of cytoplasmic membrane markers. The genetics, as well as the subcellular localization of Cyc2 at the outer membrane level, therefore make it a prime candidate for the initial electron acceptor in the respiratory pathway between ferrous iron and oxygen.

Empirical ferric iron corrections: necessity, assumptions, and effects on selected geothermobarometers

1991 ◽  
Vol 55 (378) ◽  
pp. 3-18 ◽  
Author(s):  
John C. Schumacher
Keyword(s):  
Ferrous Iron ◽  
Ferric Iron ◽  
Bulk Composition ◽  
Total Iron ◽  
Chemical Analyses ◽  
Treatment Results ◽  
Ideal Stoichiometry ◽  
End Members ◽  
Excess Cations ◽  
Mineral Analyses

AbstractThe ferromagnesian silicate minerals, such as garnets, pyroxenes, micas, and amphiboles, appear in a variety of geothermometers and geobarometers. Where complete chemical analyses are available and regardless of bulk composition (metamorphosed pelitic or mafic), the aforementioned minerals commonly contain ferric iron. In mineral analyses using the electron microprobe, ferric and ferrous iron are not distinguished, and all the iron is treated as FeO. In ferric Fe-bearing minerals, this treatment results in (1) low analytical sums and (2) excess cations in the mineral formulae. Assuming ideal stoichiometry (ideal formula cations and oxygens) allows direct ferric estimates in garnets and pyroxenes; amphiboles require additional assumptions concerning site occupancies, and, for micas, no acceptable constraint exists for a ferric estimate. Based on ferric iron determinations for some metamorphic ferromagnesian silicates, the proportion of ferric to total iron increases at higher XMg values. The influence of ferric estimates on T and P calculations depends on the model used and on the extent the ferric estimate alters the relative proportions of end-members. Several examples suggest that, in general, if ferric estimates (or determinations) are made, they should be made for all the relevant minerals.

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