Comparison of two different box styles for mass rearing of Sasajiscymnus tsugae (Coleoptera: Coccinellidae), a biological control agent of hemlock woolly adelgid (Hemiptera: Adelgidae)
AbstractThe hemlock woolly adelgid (HWA), Adelges tsugae Annand, is the number one threat to hemlock (genus Tsuga Carr.; Pinaceae) forests in eastern North America. Sasajiscymnus tsugae (Sasaji et McClure), an introduced coccinellid from Japan, is a promising biological control agent for HWA. In mass rearing of S. tsugae, the main goals are to maximize adult beetle production, reduce the amount of human labor, and minimize production costs. Significantly higher adult S. tsugae production and survivorship from egg to adult were observed in a modified rearing box compared with a conventional rearing box. The 30 modified boxes produced 4400 more beetles than the 30 conventional boxes. Over time, egg to adult survivorship decreased because of larval cannibalism at densities above 1650 larvae per rearing box. Additionally, warmer weather conditions increased the number of insect and spider predators incidentally introduced via HWA-infested hemlock twigs. There was no significant difference in the time required to prepare modified and conventional boxes for initial introduction of eggs, but significantly less time was required to add water, honey, and HWA-infested twigs to the modified boxes. The additional cost and time required to add modifications to each box was compensated for by the increased number of adult S. tsugae produced and the time saved during the 35 days of scheduled maintenance as eggs hatched and larvae developed to adults.