scholarly journals Engineering of Isogenic Cells Deficient for MR1 with a CRISPR/Cas9 Lentiviral System: Tools To Study Microbial Antigen Processing and Presentation to Human MR1-Restricted T Cells

2016 ◽  
Vol 197 (3) ◽  
pp. 971-982 ◽  
Author(s):  
Bruno Laugel ◽  
Angharad Lloyd ◽  
Erin W. Meermeier ◽  
Michael D. Crowther ◽  
Thomas R. Connor ◽  
...  
Keyword(s):  
T Cells ◽  
Antigen Processing ◽  
Microbial Antigen ◽  
Antigen Processing And Presentation

scholarly journals Sublethal Radiation Affects Antigen Processing and Presentation Genes to Enhance Immunogenicity of Cancer Cells

2020 ◽  
Vol 21 (7) ◽  
pp. 2573 ◽  
Author(s):  
Achamaporn Punnanitinont ◽  
Eric D. Kannisto ◽  
Junko Matsuzaki ◽  
Kunle Odunsi ◽  
Sai Yendamuri ◽  
...  
Keyword(s):  
New York ◽  
T Cells ◽  
Cancer Cells ◽  
Cell Lines ◽  
Antigen Processing ◽  
Tumor Antigens ◽  
T Cell Response ◽  
Live Cells ◽  
X Rays ◽  
Antigen Processing And Presentation

While immunotherapy in cancer is designed to stimulate effector T cell response, tumor-associated antigens have to be presented on malignant cells at a sufficient level for recognition of cancer by T cells. Recent studies suggest that radiotherapy enhances the anti-cancer immune response and also improves the efficacy of immunotherapy. To understand the molecular basis of such observations, we examined the effect of ionizing X-rays on tumor antigens and their presentation in a set of nine human cell lines representing cancers of the esophagus, lung, and head and neck. A single dose of 7.5 or 15 Gy radiation enhanced the New York esophageal squamous cell carcinoma 1 (NY-ESO-1) tumor-antigen-mediated recognition of cancer cells by NY-ESO-1-specific CD8+ T cells. Irradiation led to significant enlargement of live cells after four days, and microscopy and flow cytometry revealed multinucleation and polyploidy in the cells because of dysregulated mitosis, which was also revealed in RNA-sequencing-based transcriptome profiles of cells. Transcriptome analyses also showed that while radiation had no universal effect on genes encoding tumor antigens, it upregulated the expression of numerous genes involved in antigen processing and presentation pathways in all cell lines. This effect may explain the immunostimulatory role of cancer radiotherapy.

Sphingosine 1-phosphate promotes antigen processing and presentation to CD4+ T cells in Mycobacterium tuberculosis-infected monocytes

2007 ◽  
Vol 361 (3) ◽  
pp. 687-693 ◽  
Author(s):  
Marilina B. Santucci ◽  
Emanuela Greco ◽  
Marco De Spirito ◽  
Giuseppe Arcovito ◽  
Giulia De Angelis ◽  
...  
Keyword(s):  
T Cells ◽  
Mycobacterium Tuberculosis ◽  
Cd4 T Cells ◽  
Antigen Processing ◽  
Sphingosine 1 Phosphate ◽  
Antigen Processing And Presentation

The binary logic of antigen processing and presentation to T cells

Cell ◽  
1990 ◽  
Vol 62 (2) ◽  
pp. 203-206 ◽  
Author(s):  
Jonathan W. Yewdell ◽  
Jack R. Bennink
Keyword(s):  
T Cells ◽  
Antigen Processing ◽  
Binary Logic ◽  
Antigen Processing And Presentation

scholarly journals Oxidative stress impairs intracellular events involved in antigen processing and presentation to T cells

2003 ◽  
Vol 8 (2) ◽  
pp. 162 ◽  
Author(s):  
Olivier Preynat-Seauve ◽  
Sylvia Coudurier ◽  
Alain Favier ◽  
Patrice-Noël Marche ◽  
Christian Villiers
Keyword(s):  
Oxidative Stress ◽  
T Cells ◽  
Antigen Processing ◽  
Antigen Processing And Presentation

scholarly journals Analysis of the Expression and Prognosis for Leukocyte Immunoglobulin-Like Receptor Subfamily B in Human Liver Cancer

2021 ◽  
Author(s):  
Jing Fan ◽  
Lili Wang ◽  
Miao Chen ◽  
Jiakang Zhang ◽  
Jiayan Li ◽  
...  
Keyword(s):  
T Cells ◽  
Liver Cancer ◽  
Natural Killer Cell ◽  
Mrna Expression ◽  
Cancer Patients ◽  
Antigen Processing ◽  
Killer Cell ◽  
Family Members ◽  
Cancer Tissues ◽  
Antigen Processing And Presentation

Abstract Background: Leukocyte immunoglobulin-like receptor subfamily B (LILRB), including 5 subtypes, is a group of inhibitory receptors in immune system. The LILRB family is known to be involved in the tumor progression of various cancer types, especially liver cancer. However, the expression patterns and prognostic values of LILRB family members in liver cancer tissues remain unclear.Methods: We used the Oncomine database, GEPIA database, Kaplan–Meier Plotter, Timer, TISIDB and cBioPortal to assess the expression and prognostic value of the LILRB family in liver cancer patients. We also verified the expression of the LILRB family in tumor tissues and tumor-free liver tissues at the protein level by using immunohistochemistry. The STRING website was used to explore the interaction between the LILRB family and their related genes. The DAVID database was used to perform the GO and KEGG analyses. Flow cytometry was used to assess the infiltrated NK cells in liver cancer tissues.Results: Our study revealed that the mRNA expression of LILRB1, LILRB2, LILRB3 and LILRB5 was downregulated, while compared with normal tissues, the mRNA expression of LILRB4 was upregulated in liver cancer tissues. Survival analysis revealed that LILRB2 and LILRB5 mRNA expression levels were significantly associated with OS and DSS and that the mRNA expression of all LILRB family members was significantly correlated with RFS and PFS. Next, we further found that the mRNA expression of all LILRB family members was significantly associated with the infiltration of B cells, CD8+ T cells, CD4+ T cells, macrophages, and neutrophil dendritic cells in liver cancer. Finally, GO and KEGG analyses found that the LILRB family and its related genes were involved in antigen processing and presentation and natural killer cell-mediated cytotoxicity pathways.Conclusions: Our study suggested that LILRB family expression was associated with the prognosis of liver cancer patients and infiltrated immune cells. The LILRB family might be involved in antigen processing and presentation and natural killer cell-mediated cytotoxicity pathways.

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