scholarly journals Development of an in vitro cell culture model to study milk to plasma ratios of therapeutic drugs

2013 ◽  
Vol 45 (4) ◽  
pp. 325 ◽  
Author(s):  
Anurupa Maitra ◽  
Shahnaz Patel ◽  
VijayR Bhate ◽  
VilliS Toddywalla ◽  
MaithiliA Athavale
Keyword(s):  
Cell Culture ◽  
Cell Culture Model ◽  
In Vitro Cell Culture ◽  
Therapeutic Drugs ◽  
Culture Model

scholarly journals An In Vitro Cell Culture Model for Pyoverdine-Mediated Virulence

Pathogens ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 9
Author(s):  
Donghoon Kang ◽  
Natalia V. Kirienko
Keyword(s):  
Cell Culture ◽  
Virulence Factors ◽  
Model Organisms ◽  
Cell Culture Model ◽  
Chronic Infections ◽  
In Vitro Cell Culture ◽  
Mitochondrial Homeostasis ◽  
Culture Model ◽  
Wide Range

Pseudomonas aeruginosa is a multidrug-resistant, opportunistic pathogen that utilizes a wide-range of virulence factors to cause acute, life-threatening infections in immunocompromised patients, especially those in intensive care units. It also causes debilitating chronic infections that shorten lives and worsen the quality of life for cystic fibrosis patients. One of the key virulence factors in P. aeruginosa is the siderophore pyoverdine, which provides the pathogen with iron during infection, regulates the production of secreted toxins, and disrupts host iron and mitochondrial homeostasis. These roles have been characterized in model organisms such as Caenorhabditis elegans and mice. However, an intermediary system, using cell culture to investigate the activity of this siderophore has been absent. In this report, we describe such a system, using murine macrophages treated with pyoverdine. We demonstrate that pyoverdine-rich filtrates from P. aeruginosa exhibit substantial cytotoxicity, and that the inhibition of pyoverdine production (genetic or chemical) is sufficient to mitigate virulence. Furthermore, consistent with previous observations made in C. elegans, pyoverdine translocates into cells and disrupts host mitochondrial homeostasis. Most importantly, we observe a strong correlation between pyoverdine production and virulence in P. aeruginosa clinical isolates, confirming pyoverdine’s value as a promising target for therapeutic intervention. This in vitro cell culture model will allow rapid validation of pyoverdine antivirulents in a simple but physiologically relevant manner.

scholarly journals 2-Deoxy-D-Glucose Attenuates Isoflurane-Induced Cytotoxicity in an In Vitro Cell Culture Model of H4 Human Neuroglioma Cells

2011 ◽  
Vol 113 (6) ◽  
pp. 1468-1475 ◽  
Author(s):  
Jun Zhang ◽  
Yuanlin Dong ◽  
Zhipeng Xu ◽  
Yiying Zhang ◽  
Chuxiong Pan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Culture Model ◽  
In Vitro Cell Culture ◽  
Culture Model

Permeability Characteristics of Endocrine-Disrupting Chemicals Using an In Vitro Cell Culture Model, Caco-2 Cells

2002 ◽  
Vol 3 (5) ◽  
pp. 551-557 ◽  
Author(s):  
Yukako Yoshikawa ◽  
Ayako Hayashi ◽  
Maya Inai ◽  
Akiko Matsushita ◽  
Nobuhito Shibata ◽  
...  
Keyword(s):  
Cell Culture ◽  
Endocrine Disrupting Chemicals ◽  
Cell Culture Model ◽  
Permeability Characteristics ◽  
In Vitro Cell Culture ◽  
Culture Model ◽  
Endocrine Disrupting
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