scholarly journals Micro-computed tomography evaluation of microleakage of Class II composite restorations: An in vitro study

2018 ◽  
Vol 12 (03) ◽  
pp. 369-374 ◽  
Author(s):  
Angelo Zavattini ◽  
Manuele Mancini ◽  
James Higginson ◽  
Federico Foschi ◽  
Guido Pasquantonio ◽  
...  

ABSTRACT Objective: The aim of this study is to investigate the microleakage attained with three resin-based material used to restore deep Class II cavities. A null hypothesis was chosen: there is no difference in microleakage among the tested materials. Materials and Methods: A total of 30 Class II cavities were prepared in freshly extracted molars with the proximal mesial and distal margins located, respectively, 1.5 mm apically and 1.5 mm coronally to the cementum-enamel junction. Restorations were completed using a three-step enamel-dentin adhesive system “Etch and Rinse,” margins were relocated using a micro-hybrid, preheated, or flowable composite and restorations were then completed using a conventional composite. All samples were coated with nail varnish with the exception of an area along the margins and apex was sealed using epoxide cement and then thermocycled (30-s dwell time, 5°C/55°C, 1000 cycles). A 50% ammoniac AgNO3 solution was used as tracer according to Tay's protocol. The microleakage analysis was performed using a microtomography system Sky-scan 1072 (SKYSCAN, Kartuizersweg 3B 2550, Konitch, Belgium). Results: The mean microleakage of all the tested materials showed greater leakage in the cementum margins; flowable composite exhibit greater leakage among the groups. Significant differences (P < 5%) within groups in both enamel and dentin margins were present. None of the tested materials eliminated marginal microleakage. Preheated composite showed significantly lesser microleakage. Conclusion: Tested materials showed statistical differences in microleakage; thus, the null hypothesis has been rejected. Within the limitations of the present experimental procedure, it can be concluded that flowable resin composite should be avoided at the dentin/cementum margin.

2013 ◽  
Vol 14 (1) ◽  
pp. 56-60 ◽  
Author(s):  
S Nagalaxmi Reddy ◽  
DN Jayashankar ◽  
Mohanthomas Nainan

ABSTRACT Aim Aim of this study was to compare class II composite restoration using flowable composites as lining with various thickness and curing techniques by evaluating cervical marginal microleakage. Materials and methods Fifty intact molars, each prepared with two box-only class II cavities, were randomly divided into five groups: Group I, P60 filling alone; group II, ultrathin flowable composite lining (0.5-1mm) cocured with overlying composite; group III, thin lining (1-1.5) cocured with overlying composite; group IV, ultrathin lining (0.5-1 mm) precured and group V, thin lining (1-1.5) precured. The teeth were then thermocycled for 1500 cycles (between 5 and 60 °C) and immersed in dye for 24 hours. Cervical microleakage was measured as the extent of dye penetration. Results Cocured specimens (groups II and III) showed least microleakage and control specimens (group I) showed maximum microleakage. On comparison of curing techniques, cocured specimens (groups II and III) showed less microleakage than precured (groups IV and V). On comparison of lining thickness and type of curing, group IV showed less microleakage than group V. Groups II and III were statistically not significant. Conclusion It was concluded from the results that ultrathin cocured flowable composite lining specimens improved the marginal sealing with decreased microleakage. Clinical significance In this study, the application of additional flowable composite lining with various thicknesses presented different influences in marginal quality of class II box only composite restorations. A new technique applying an ultrathin flowable composite lining with cocuring technique improved the marginal sealing with decreased microleakage. Restorations with thin lining presented reduced marginal integrity after thermocycling. How to cite this article Reddy SN, Jayashankar DN, Nainan M, Shivanna V. The Effect of Flowable Composite Lining Thickness with Various Curing Techniques on Microleakage in Class II Composite Restorations: An in vitro Study. J Contemp Dent Pract 2013;14(1):56-60.


2018 ◽  
Vol 43 (4) ◽  
pp. 426-436 ◽  
Author(s):  
J Li ◽  
B Chen ◽  
N Hong ◽  
S Wu ◽  
Y Li

SUMMARY Objective: In an attempt to increase resin-dentin bonding quality, this study used baicalein as a preconditioner in an etch-and-rinse adhesive to evaluate its effect on matrix metalloproteinases (MMPs) and adhesive durability. Methods: As a MMP inhibitor and potential collagen cross-linking agent, baicalein was used as a preconditioner in an etch-and-rinse adhesive system. The degree of conversion was evaluated by Fourier-transform infrared spectroscopy. EnzChek gelatinase/collagenase assay kits were then used to detect the MMP inhibitory effect of different concentrations of baicalein (0.1, 0.5, 2.5, and 5.0 μg/mL) on dentin powders. During in vitro bonding procedures, flat dentin surfaces on sound third molars were preconditioned with 2.5 μg/mL baicalein after being acid-etched; this step was followed by continuation of adhesive processes and build-up of resin composite. After resin-dentin stick preparation, bonding strength, failure mode, and interface nanoleakage were respectively evaluated via microtensile testing, stereomicroscopy, and field emission scanning electron microscopy either immediately or after storage in artificial saliva for three or six months. Data were analyzed by two-way analysis of variance and Tukey test (α=0.05). Results: Baicalein at a concentration of 0-5.0 μg/mL did not influence the conversion of adhesives. However, it inhibited the activities of dentin-bond gelatinase and collagenase, especially at a concentration of 2.5 μg/mL, while effectively increasing microtensile bonding strength and decreasing nanoleakage in vitro, both immediately and after aging. Conclusions: Baicalein used as preconditioner in an etch-and-rinse adhesive system has an anti-MMP function and effectively improves resin-dentin bonding durability in vitro, which has potential value in clinical bonding procedures.


2014 ◽  
Vol 39 (1) ◽  
pp. 64-71 ◽  
Author(s):  
DMS Simões ◽  
RT Basting ◽  
FLB Amaral ◽  
CP Turssi ◽  
FMG França

SUMMARY The aim of this study was to evaluate the effect of a chlorhexidine and/or ethanol application on the bond strength of an etch-and-rinse, hydrophobic adhesive system either under in vitro aging or in situ cariogenic challenge. The dentin surface of 36 human third molars were flattened and allocated into four groups to be treated with chlorhexidine, ethanol, or chlorhexidine + ethanol or left unexposed to any solution (control) (n=9). Then, a resin composite restoration was made on the dentin surface and longitudinal sticks were obtained. Sticks from each tooth were assigned to three test conditions: stored in water in vitro for 24 hours, stored in water in vitro for 6 months, or worn in situ for 14 days. During in situ wear time, a high-cariogenic challenge condition was simulated. Specimens were tested for microtensile bond strength (μTBS). Multivariate analysis of variance and Tukey's test showed that chlorhexidine, ethanol, or chlorhexidine + ethanol did not affect the μTBS. The in vitro μTBS values were significantly lower for the specimens stored for 6 months than for those stored for 24 hours. Intermediate μTBS values were shown by the specimens worn in situ. Thus, use of chlorhexidine and/or ethanol was incapable of containing the degradation at the bond interface in the in vitro model. The in situ model was capable of reducing bond strength similarly to the in vitro/6 months model. Despite this, the in situ bond strength was still similar to that of the in vitro/24-hour model.


2004 ◽  
Vol 15 (3) ◽  
pp. 194-198 ◽  
Author(s):  
Vanessa Gallego Arias ◽  
Inger Teixeira Campos ◽  
Luiz André Freire Pimenta

The purpose of this in vitro study was to evaluate the efficacy of three hydrophilic dentin adhesive systems to reduce class II restoration microleakage. A total of 60 human molar teeth were used in which two box cavities were made on the distal and mesial surfaces, with a cervical margin in dentin. These cavities were randomly divided into 3 groups (n = 40 each), according to adhesive system tested: G1: OptiBond SOLO®; G2: Amalgambond Plus®; G3: Etch & Prime 3.0®. The cavities were restored with the composite resin Z-100®. The groups were thermocycled 2000 times (5 ± 1ºC and 55 ± 1ºC) with a dwell time of 1 min. The teeth were then immersed in 2% methylene blue, pH 7.0, for 4 h, sectioned and observed with a stereomicroscope MEIJI 2000 (35X). The evaluation was made using scores (0-4) and the results were expressed through the sum of the ranks. G1 = 1994.00; G2 = 2294.00; G3 = 2972.00. The three groups were significantly different. The self-etching adhesive system Etch & Prime 3.0 was less effective in preventing microleakage. The OptiBond SOLO adhesive was the most effective in reducing microleakage in dentin margins when compared with Amalgambond Plus and Etch & Prime 3.0.


2003 ◽  
Vol 11 (4) ◽  
pp. 342-347 ◽  
Author(s):  
Marcelo Giannini ◽  
Patrícia Chaves ◽  
Marcelo Tavares de Oliveira

This in vitro study evaluated the effect of tooth age on the tensile bond strength of Prime & Bond NT adhesive system to dentin. Human third molars from the five age groups were analyzed: A- 17 to 20yrs, B- 21 to 30yrs, C- 31 to 40yrs, D- 41 to 50yrs and E- 51 to 63yrs. The occlusal enamel was removed using a diamond saw under water cooling and the dentin surface was wet-ground with 600-grit SiC paper to obtain flat surfaces. The adhesive system was applied according to the manufacturer's instructions and a 6-mm high resin "crown" was built-up with resin composite. Teeth were stored for 24 hours in distilled water at 37ºC and prepared for micro-tensile testing. Each specimen was mounted in a testing jig attached to a universal testing machine and stressed in tension at a crosshead speed of 0.5mm/min until failure. The means of tensile bond strength were (MPa): A- 21.42 ± 7.52ª; B- 30.13 ± 10.19ª; C- 31.69 ± 11.78ª; D- 30.69 ± 8.47ª and E- 35.66 ± 9.54ª. No statistically significant difference was observed among the age groups (p > 0.05). The results suggested that the tensile bond strength of the adhesive system was not significantly affected by dentin aging.


2012 ◽  
Vol 37 (2) ◽  
pp. 205-210 ◽  
Author(s):  
H El-Shamy ◽  
MH Saber ◽  
CE Dörfer ◽  
W El-Badrawy ◽  
BAC Loomans

SUMMARY Background Proximal contact tightness of class II resin composite restorations is influenced by a myriad of factors. Previous studies investigated the role of matrix band type and thickness, consistency of resin composite, and technique of placement. However, the effect of volumetric shrinkage of resin and intensity of curing light has yet to be determined. Thus, the aim of this study was to identify the influence of these factors on the proximal contact tightness when restoring class II cavity preparations in vitro. Methods Sixty artificial molars were restored with either a low-shrinkage (Filtek Silorane, 3M ESPE) or a conventional (Z100, 3M ESPE) composite and polymerized with low-intensity (Smartlite IQ2, Dentsply) or high-intensity light curing units (DemiTM, Kerr). Proximal contact tightness was measured using the Tooth Pressure Meter. Data were statistically analyzed using one-way analysis of variance and Tukey post hoc test. Results Use of low-shrinkage composite (Filtek Silorane) resulted in significantly tighter proximal contacts compared to the use of conventional composite (Z100) when cured with the same polymerization unit (p&lt;0.001). Moreover, the low-intensity curing unit (IQ2) resulted in significantly tighter contacts than the high-intensity unit when material is constant (p&lt;0.001). Conclusions Low-shrinkage resin composite and low curing light intensity is associated with tighter proximal contact values.


Materials ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4299
Author(s):  
Andreea Ciurea ◽  
Ada Gabriela Delean ◽  
Andreea Didilescu ◽  
Monica Monea ◽  
Alexandra Roman ◽  
...  

The study aimed to evaluate the adhesive performances of two ormocer materials and two micro-hybrid composites placed to restore class II cavities. We tested the null hypothesis, which considered that the adhesive behaviors of tested materials did not differ. On each extracted tooth, two class II cavities were prepared having an enamel located cervical margin and a cementum located cervical margin, respectively, and were restored using two different restoration techniques. The teeth followed a tooth impregnating protocol and were sectioned and evaluated by optical microscopy to highlight the marginal microleakage around restorations. Cervical and occlusal microleakage as well as microleakage ratios were calculated. The microleakage test showed that all tested materials exhibited some degree of dentinal microleakage both on cervical and occlusal areas irrespective of the restoration technique. Some significant differences were recorded in adhesion performance of the materials. The cervical microleakage ratio was significantly increased for one of the micro-hybrid resin composites in comparison with one of the ormocer materials (p = 0.0159). Significantly differences were observed in occlusal microleakage ratios when the two micro-hybrid composites were compared (p = 0.047). The results failed to reject the null hypothesis. The present study could not demonstrate the superiority of ormocer-materials relative to conventional composites.


2019 ◽  
Vol 10 (2) ◽  
Author(s):  
Ognjenka Janković ◽  
Smiljana Paraš ◽  
Radmila Arbutina ◽  
Irena Kuzmanović Radman ◽  
Tijana Adamović ◽  
...  

Introduction: Evaluation of microleakage is important for assessing the success of new restorative materials and methods. The aim of this study was to assess the microleakage of class II restorations with different flowable composites as liners and two different polymerization techniques classic and soft start. Materials and Methods: 40 extracted human premolars teeth with class II cavity preparation medial and distal (80 cavities) were divided into four groups: 1.Vertise Flow (VF)+micro hybrid composite Herculite 2.Surefil SDR Flow (SDRF)+micro-hybrid composite Herculite 3.Tetric Flow (TF)+micro-hybrid composite Herculite 4.control group micro-hybrid composite Herculite. Mesial cavities are polymerized with classic and distal cavity with soft start technique of polymerization. After that, the samples were immersed in 0.5% AgNO3 solution and sectioned into the mesiodistal direction. Using a stereomicroscope (Nikon - Japan), with a magnification of 40x, the gingival microleakage of cavities was examined. Data were analyzed using Fisher's and Student's tests. Results: After using the classical polymerization technique, all three used flowable composites VF+Herculite, SDRF+Herculite, TF+Herculite showed less gingival microleakage than the control group. This difference was statistically significant. After the application of the soft-start technique of polymerization, VF+Herculite and SDRF+Hercules showed a statistically significant reduction in gingival microleakage, while TF+Herculite showed a comparable result with control group, without a statistically significant difference. There was no statistically significant difference between classical and soft start polymerization techniques. Conclusion: Flowable composites in this study have reduced gingival microleakage and can be used as liners in the restoration of II class cavities.


2021 ◽  
Vol 11 (4) ◽  
pp. 306-312
Author(s):  
Dalia M. Abouelmagd ◽  
Hanan K. Abouelseoud

Objectives: This study aimed to compare the microshear bond strength (µSBS) of self-adhesive flowable composite and conventional bonded flowable composite to sound and caries affected dentin. Materials and Methods: In this in vitro study, a total of freshly extracted 40 human molar teeth were used. The occlusal surface was sectioned to expose dentin for µSBS test. The teeth were randomly divided into 2 groups according to the type of flowable composite used into two groups (n=20): group A: self- adhesive flowable composite (Vertise flow), group B: Filtek Z350-XT flowable composite and one-step self-etch adhesive Adper Easy One. A plastic cylindrical mold was used to construct resin composite cylinders (0.8mm diameter x 1mm length) on the sound and caries affected exposed dentin and filled with flowable composite restorative materials according to manufacturer’s instructions. The samples were then immersed in distilled water at 37°C for 24 hours, and underwent µSBS test at a crosshead speed of 0.5 mm/min. Data were analyzed using Student's t-test to compare between the two materials as well as to compare between sound and caries affected dentin. Results: The mean values of µSBS of Filtek Z-350XT to sound and caries affected were 26.6 and 26.7 MPa respectively. The mean µSBS of Vertise Flow to sound and affected were 13.2 and 17.9 MPa respectively. With sound dentin FiltekZ-350XT showed statistically significant higher µSBS than Vertise flow. With caries affected dentin, there was no statistically significant difference between the two materials. There was no statistically significant difference between µSBS of sound and caries affected dentin within each material. The significance level was set at P ≤ 0.05. Conclusions: Within the limitations of this study, the conventional flowable composite yielded the highest µSBS to sound dentin. There was no statistical significant difference in µSBS of self-adhesive and conventional flowable to caries affected dentin. Key words: Composite Resins; Dentin; microshear.


2016 ◽  
Vol 63 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Navjot S. Mann ◽  
Sameer Makkar ◽  
Reetika Sharma

SUMMARY Introduction The aim of this study was to evaluate marginal sealing ability of newly introduced Dyad Flow flowable composite and compare to total-etch and self-etch adhesive system at the coronal and apical margins of class V resin composite restorations. Material and Methods A standard class V cavity (3 mm mesiodistal width, 3 mm occlusogingival height and 1.5 mm axial depth) was prepared on the buccal surface of freshly extracted sound human teeth. Occlusal and gingival margins of the cavities were located in enamel and cementum/dentin, respectively. Teeth were randomly assigned into the three groups (n=10) and restored with different composite materials following the manufacturer’s instructions: group I was restored with nanohybrid resin composite using total-etch bonding agent; group II was restored with nanohybrid resin composite using self-etch technique; group III was restored with flowable composite (Dyad Flow), respectively. After finishing and polishing, the teeth were coated with nail varnish and immersed in rhodamine B dye and sectioned longitudinally. Dye penetration was examined under stereomicroscope and scored separately for occlusal and gingival margins on a 0–3 ordinal scale. Data were analyzed with Kruskal–Wallis, Mann–Whitney and Wilcoxon tests (a=0.05). Result Statistical analysis showed that specimens restored with the total-etch and self-etch adhesive systems revealed reduced leakage at the coronal margin. At the apical margin, Dyad Flow showed greater leakage than other groups. Conclusion Newly introduced flowable composite Dyad Flow showed inferior adhesive bond with enamel and dentin compared to total-etch and self-etch techniques.


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