Development of an agronomically superior blackleg resistant canola cultivar in Brassica napus L. using doubled haploidy

1995 ◽  
Vol 75 (2) ◽  
pp. 437-439 ◽  
Author(s):  
G. R. Stringam ◽  
V. K. Bansal ◽  
M. R. Thiagarajah ◽  
D. F. Degenhardt ◽  
J. P. Tewari
Keyword(s):  
Brassica Napus ◽  
Doubled Haploid ◽  
Leptosphaeria Maculans ◽  
Resistance Breeding ◽  
Breeding Method ◽  
Brassica Napus L ◽  
Breeding Lines ◽  
University Of Alberta ◽  
Blackleg Resistance ◽  
The University

The doubled haploid breeding method and greenhouse screening using cotyledon bio-assay were successfully applied to transfer blackleg resistance from the Australian cultivar Maluka (Brassicas napus), into susceptible advanced B. napus lines from the University of Alberta. This approach for blackleg resistance breeding was effective and efficient as several superior blackleg resistant breeding lines were identified within 4 yr from the initial cross. One of these lines (91–21864NA) was entered in the 1993 trials of the Western Canada Canola/Rapeseed Recommending Committee. Key words: Blackleg resistance, Leptosphaeria maculans, doubled haploid, Brassica napus

Identification and mapping of a third blackleg resistance locus in Brassica napus derived from B. rapa subsp. sylvestris

Genome ◽  
2008 ◽  
Vol 51 (1) ◽  
pp. 64-72 ◽  
Author(s):  
Fengqun Yu ◽  
Derek J. Lydiate ◽  
S. Roger Rimmer
Keyword(s):  
Brassica Napus ◽  
Linkage Group ◽  
Resistance Gene ◽  
Leptosphaeria Maculans ◽  
Resistance Locus ◽  
Dominant Allele ◽  
Breeding Lines ◽  
Disease Reaction ◽  
Blackleg Resistance ◽  
Map Location

The spectrum of resistance to isolates of Leptosphaeria maculans and the map location of a new blackleg resistance gene found in the canola cultivar Brassica napus ‘Surpass 400’ are described. Two blackleg resistance genes, LepR1 and LepR2, from B. rapa subsp. sylvestris and introgressed in B. napus were identified previously. ‘Surpass 400’ also has blackleg resistance introgressed from B. rapa subsp. sylvestris. Using 31 diverse isolates of L. maculans, the disease reaction of ‘Surpass 400’ was compared with those of the resistant breeding lines AD9 (which contains LepR1), AD49 (which contains LepR2), and MC1-8 (which contains both LepR1 and LepR2). The disease reaction on ‘Surpass 400’ was different from those observed on AD9 and MC1-8, indicating that ‘Surpass 400’ carries neither LepR1 nor both LepR1 and LepR2 in combination. Disease reactions of ‘Surpass 400’ to most of the isolates tested were indistinguishable from those of AD49, which suggested ‘Surpass 400’ might contain LepR2 or a similar resistance gene. Classical genetic analysis of F1 and BC1 plants showed that a dominant allele conferred resistance to isolates of L. maculans in ‘Surpass 400’. The resistance gene, which mapped to B. napus linkage group N10 in an interval of 2.9 cM flanked by microsatellite markers sR12281a and sN2428Rb and 11.7 cM below LepR2, was designated LepR3. A 9 cM region of the B. napus genome containing LepR3 was found to be syntenic with a segment of Arabidopsis chromosome 5.

scholarly journals Sensitivity of winter rapeseed (Brassica napus L.) to isolates of the fungus Leptosphaeria maculans (Desm.) Ces. et de Not

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 572-574
Author(s):  
E. Plachká ◽  
L. Odstrčilová
Keyword(s):  
Plant Pathology ◽  
Brassica Napus ◽  
Leptosphaeria Maculans ◽  
Brassica Napus L ◽  
Yellow Colour ◽  
Breeding Lines ◽  
University Of Wisconsin ◽  
Pure Cultures ◽  
Winter Rapeseed ◽  
And Control

The pure cultures of the fungus Leptosphaeria maculans were isolated from the rapeseed leaves collected in Moravia. Two isolates were collected on the basis of their ability to produce yellow colour. The isolate producing yellow colour was classified as aggressive, while the isolate without the ability to produce yellow colour was not aggressive. The sensitivity to these isolates was verified in 11 rapeseed genotypes. This test was based on the method from the document Crucifer Genetics Cooperative (information document from the Department of Plant Pathology, University of Wisconsin). This method is based on the inoculation of cruciferous leaflets with suspension from the isolate of the pathogen observed. The scale from 0 to 9 was used for evaluation (0 = no darkening around the wound, 9 = accompanied by profuse sporulation in large, more than 5 mm, lesions with diffuse margins). The infection degree of the aggressive isolate ranged in different genotypes from 1.45 to 4.5, the results of the non-aggressive isolate ranged from 1.0 to 1.5. Based on these results the aggressive isolate was used for further tests of the selected breeding lines. The tests involved selected breeding materials, registered varieties, control varieties with different sensitivity to the isolate of Leptosphaeria maculans and control varieties included in state trials. The maximal difference in the sensitivity exhibited by individual genotypes was five degrees.

scholarly journals Stabilization of Resistance to Leptosphaeria maculans in Brassica napus–B. juncea Recombinant Lines and Its Introgression into Spring-Type Brassica napus

Plant Disease ◽  
2008 ◽  
Vol 92 (8) ◽  
pp. 1208-1214 ◽  
Author(s):  
A.-M. Chèvre ◽  
H. Brun ◽  
F. Eber ◽  
J.-C. Letanneur ◽  
P. Vallee ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Dominant Gene ◽  
Leptosphaeria Maculans ◽  
Polygenic Resistance ◽  
Breeding Lines ◽  
Blackleg Resistance ◽  
Backcross Populations ◽  
Spring Type ◽  
The Stability ◽  
High Level

The value of Katanning Early Maturing (KEM) breeding lines from Western Australia, derived from Brassica napus × B. juncea crosses, was assessed as a source of germplasm for resistance to blackleg disease (caused by Leptosphaeria maculans) in spring-type oilseed rape cultivars. The stability of blackleg resistance in these KEM lines was related to key cytological characteristics to determine why there are poor levels of introgression of this resistance into progeny. Promising recombinant KEM lines were crossed with the spring-type B. napus cv. Dunkeld, which has useful polygenic resistance to blackleg, and screened for resistance. The lines were analyzed cytologically for pairing of bivalents in each generation to aid in the selection of stable recombinant lines. KEM recombinant lines showing regular meiotic behavior and a high level of blackleg resistance were obtained for the first time. We also showed that the stable introgression of the B. juncea resistance from the KEM lines into a ‘Dunkeld’ background was possible. Inoculation of selfing and backcross populations with isolates of L. maculans having different AvrLm genes indicated that the B. juncea resistance gene, Rlm6, had been introgressed into a B. napus spring-type cultivar carrying polygenic resistance. The combination of both resistances would enhance the overall effectiveness of resistance against L. maculans. This is clearly needed in Australia and France where cultivars relying upon single dominant gene-based resistance for their effectiveness have proved not durable.

scholarly journals Recent Findings Unravel Genes and Genetic Factors Underlying Leptosphaeria maculans Resistance in Brassica napus and Its Relatives

2020 ◽  
Vol 22 (1) ◽  
pp. 313
Author(s):  
Aldrin Y. Cantila ◽  
Nur Shuhadah Mohd Saad ◽  
Junrey C. Amas ◽  
David Edwards ◽  
Jacqueline Batley
Keyword(s):  
Brassica Napus ◽  
Genetic Factors ◽  
Quantitative Resistance ◽  
Genetic Resistance ◽  
Leptosphaeria Maculans ◽  
Gene Interaction ◽  
R Gene ◽  
R Genes ◽  
Blackleg Resistance ◽  
Avr Gene

Among the Brassica oilseeds, canola (Brassica napus) is the most economically significant globally. However, its production can be limited by blackleg disease, caused by the fungal pathogen Lepstosphaeria maculans. The deployment of resistance genes has been implemented as one of the key strategies to manage the disease. Genetic resistance against blackleg comes in two forms: qualitative resistance, controlled by a single, major resistance gene (R gene), and quantitative resistance (QR), controlled by numerous, small effect loci. R-gene-mediated blackleg resistance has been extensively studied, wherein several genomic regions harbouring R genes against L. maculans have been identified and three of these genes were cloned. These studies advance our understanding of the mechanism of R gene and pathogen avirulence (Avr) gene interaction. Notably, these studies revealed a more complex interaction than originally thought. Advances in genomics help unravel these complexities, providing insights into the genes and genetic factors towards improving blackleg resistance. Here, we aim to discuss the existing R-gene-mediated resistance, make a summary of candidate R genes against the disease, and emphasise the role of players involved in the pathogenicity and resistance. The comprehensive result will allow breeders to improve resistance to L. maculans, thereby increasing yield.

Mapping QTL controlling agronomic traits in a doubled haploid population of winter oilseed rape (Brassica napus L.)

2018 ◽  
Vol 97 (5) ◽  
pp. 1389-1406 ◽  
Author(s):  
Farshad Fattahi ◽  
Barat Ali Fakheri ◽  
Mahmood Solouki ◽  
Christian Möllers ◽  
Abbas Rezaizad
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Agronomic Traits ◽  
Doubled Haploid Population ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
Mapping Qtl ◽  
Haploid Population

scholarly journals Utilisation of doubled haploids in winter oilseed rape (Brassica napus L.) breeding

2012 ◽  
Vol 38 (No. 1) ◽  
pp. 50-54 ◽  
Author(s):  
V. Kučera ◽  
M. Vyvadilová ◽  
M. Klíma
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Doubled Haploids ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
The Czech Republic ◽  
Yield Parameters ◽  
The World

A survey of development and prospects of the utilisation of doubled haploid techniques in rapeseed breeding in the world and in the Czech Republic is presented. The first utilisation of spontaneously occurred haploids from Brassica napus inbreeding programmes is described. The development of techniques of anther and later microspore culture is outlined. Special emphasis is given to the practical use of doubled haploids for the improvement of the effectiveness of breeding new productive cultivars. Some partial results of evaluation of yield parameters and resistance to important diseases in the obtained doubled haploid lines of winter oilseed rape are shown. The literary review and present results indicate, that the doubled haploid technique can be effectively used for the development of homozygous oilseed rape lines as an alternative to conventional methods.

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