scholarly journals Use of human pluripotent stem cell-derived cells for neurodegenerative disease modeling and drug screening platform

2019 ◽  
Vol 11 (11) ◽  
pp. 1305-1322 ◽  
Author(s):  
Juan Antonio Garcia-Leon ◽  
Javier Vitorica ◽  
Antonia Gutierrez
Keyword(s):  
Stem Cell ◽  
Neurodegenerative Disease ◽  
Drug Screening ◽  
Pluripotent Stem Cell ◽  
Disease Modeling ◽  
Human Pluripotent Stem Cell ◽  
Screening Platform

scholarly journals Perspective on human pluripotent stem cell‐derived cardiomyocytes in heart disease modeling and repair

2020 ◽  
Vol 9 (10) ◽  
pp. 1121-1128
Author(s):  
Qiang Li ◽  
Jia Wang ◽  
Qiang Wu ◽  
Nan Cao ◽  
Huang‐Tian Yang
Keyword(s):  
Stem Cell ◽  
Heart Disease ◽  
Pluripotent Stem Cell ◽  
Disease Modeling ◽  
Human Pluripotent Stem Cell

scholarly journals Disease modeling of pulmonary fibrosis using human pluripotent stem cell-derived alveolar organoids

2021 ◽  
Author(s):  
Takahiro Suezawa ◽  
Shuhei Kanagaki ◽  
Keita Moriguchi ◽  
Atsushi Masui ◽  
Kazuhisa Nakao ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pulmonary Fibrosis ◽  
Pluripotent Stem Cell ◽  
Disease Modeling ◽  
Human Pluripotent Stem Cell

scholarly journals In Vivo Assessment of Size-Selective Glomerular Sieving in Transplanted Human Induced Pluripotent Stem Cell–Derived Kidney Organoids

2020 ◽  
Vol 31 (5) ◽  
pp. 921-929 ◽  
Author(s):  
Cathelijne W. van den Berg ◽  
Angela Koudijs ◽  
Laila Ritsma ◽  
Ton J. Rabelink
Keyword(s):  
Stem Cell ◽  
Molecular Mass ◽  
Glomerular Filtration ◽  
Pluripotent Stem Cell ◽  
Disease Modeling ◽  
Left Kidney ◽  
Kidney Tissue ◽  
Human Pluripotent Stem Cell ◽  
Filtration Barrier ◽  
Kidney Organoids

BackgroundThe utility of kidney organoids in regenerative medicine will rely on the functionality of the glomerular and tubular structures in these tissues. Recent studies have demonstrated the vascularization and subsequent maturation of human pluripotent stem cell–derived kidney organoids after renal subcapsular transplantation. This raises the question of whether the glomeruli also become functional upon transplantation.MethodsWe transplanted kidney organoids under the renal capsule of the left kidney in immunodeficient mice followed by the implantation of a titanium imaging window on top of the kidney organoid. To assess glomerular function in the transplanted human pluripotent stem cell–derived kidney tissue 1, 2, and 3 weeks after transplantation, we applied high-resolution intravital multiphoton imaging through the imaging window during intravenous infusion of fluorescently labeled low and high molecular mass dextran molecules or albumin.ResultsAfter vascularization, glomerular structures in the organoid displayed dextran and albumin size selectivity across their glomerular filtration barrier. We also observed evidence of proximal tubular dextran reuptake.ConclusionsOur results demonstrate that human pluripotent stem cell–derived glomeruli can develop an appropriate barrier function and discriminate between molecules of varying size. These characteristics together with tubular presence of low molecular mass dextran provide clear evidence of functional filtration. This approach to visualizing glomerular filtration function will be instrumental for translation of organoid technology for clinical applications as well as for disease modeling.

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