Optimization for the Production of Extracellular Alkaline Phosphatase from Proteus mirabilis

2015 ◽  
Vol 05 (03) ◽  
Author(s):  
Mahesh M Somashekhar R
Keyword(s):  
Alkaline Phosphatase ◽  
Proteus Mirabilis ◽  
Extracellular Alkaline Phosphatase

Extracellular alkaline phosphatase from marine bacteria: purification and properties of extracellular phosphatase from a marine Pseudomonas sp.

1980 ◽  
Vol 26 (7) ◽  
pp. 833-838 ◽  
Author(s):  
Hiromi Kobori ◽  
Nobuo Taga
Keyword(s):  
Molecular Weight ◽  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Marine Bacteria ◽  
Divalent Cations ◽  
Maximal Activity ◽  
Pseudomonas Sp ◽  
Purification And Properties ◽  
Extracellular Alkaline Phosphatase ◽  
Increased Pressure

Extracellular alkaline phosphatase produced by a marine Pseudomonas was purified to electrophoretic homogeneity. The molecular weight of the enzyme was estimated to be 100 000. The enzyme had maximal activity at pH 11.5. The enzyme was completely inhibited by 1 mM EDTA. However, divalent cations reversed the enzyme inhibition and their order of effectiveness on the reaction was Zn2+ > Ca2+ > Mn2+ > Mg2+ > Sr2+ > Co2+. The enzyme activity was affected by the species of anion whose order of effectiveness was demonstrated to follow the lyotrophic series, Cl− > Br− > NO3−> ClO4− > SCN−. The activity of phosphatase was accelerated linearly by increased pressure until up to 1000 atm (1 atm = 101.325 kPa), and the enzyme activity at 1000 atm was 3.2 times higher than that at 1 atm.

Extracellular alkaline phosphatase from the filamentous fungusAspergillus caespitosus: Purification and biochemical characterization

2003 ◽  
Vol 48 (5) ◽  
pp. 627-632 ◽  
Author(s):  
L. H. S. Guimarães ◽  
H. F. Terenzi ◽  
J. A. Jorge ◽  
F. A. Leone ◽  
M. L. T. M. Polizeli
Keyword(s):  
Alkaline Phosphatase ◽  
Biochemical Characterization ◽  
Extracellular Alkaline Phosphatase
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