scholarly journals Creating rat hepatocyte organoid as an in vitro model for drug testing

2020 ◽  
Vol 12 (10) ◽  
pp. 1184-1195
Author(s):  
Yu-Ting He ◽  
Xing-Long Zhu ◽  
Sheng-Fu Li ◽  
Bing-Qi Zhang ◽  
Yi Li ◽  
...  
Keyword(s):  
Drug Testing ◽  
In Vitro Model ◽  
Rat Hepatocyte ◽  
Vitro Model

Rat Hepatocyte Suspensions as a Suitable In Vitro Model for Studying the Biotransformation of Histone Deacetylase Inhibitors

2004 ◽  
Vol 32 (1_suppl) ◽  
pp. 105-112 ◽  
Author(s):  
Greetje Elaut ◽  
Gabriëlla Török ◽  
Peggy Papeleu ◽  
Tamara Vanhaecke ◽  
Georges Laus ◽  
...  
Keyword(s):  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitors ◽  
In Vitro Model ◽  
Rat Hepatocyte ◽  
Vitro Model

Patient-derived iPSCs, a reliable in vitro model for the investigation of Alzheimer’s disease

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Asiamah Ernest Amponsah ◽  
Ruiyun Guo ◽  
Desheng Kong ◽  
Baofeng Feng ◽  
Jingjing He ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Pluripotent Stem Cells ◽  
Drug Testing ◽  
In Vitro Model ◽  
Cell Models ◽  
Mechanistic Studies ◽  
Vitro Model ◽  
Induced Pluripotent

Abstract Alzheimer’s disease (AD) is a neurodegenerative disease and a common cause of dementia among elderly individuals. The disease is characterized by progressive cognitive decline, accumulation of senile amyloid plaques and neurofibrillary tangles, oxidative stress, and inflammation. Human-derived cell models of AD are scarce, and over the years, non-human-derived models have been developed to recapitulate clinical AD, investigate the disease’s pathogenesis and develop therapies for the disease. Several pharmacological compounds have been developed for AD based on findings from non-human-derived cell models; however, these pharmacological compounds have failed at different phases of clinical trials. This necessitates the application of human-derived cell models, such as induced pluripotent stem cells (iPSCs) in their optimized form in AD mechanistic studies and preclinical drug testing. This review provides an overview of AD and iPSCs. The AD-relevant phenotypes of iPSC-derived AD brain cells and the usefulness of iPSCs in AD are highlighted. Finally, the various recommendations that have been made to enhance iPSC/AD modelling are discussed.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

A new in vitro model for pre-transplantation diagnosis of frozen/thawed human ovarian tissue

2011 ◽  
Vol 71 (05) ◽  
Author(s):  
M Salama ◽  
K Winkler ◽  
KF Murach ◽  
S Hofer ◽  
L Wildt ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Ovarian Tissue ◽  
Human Ovarian Tissue ◽  
Vitro Model
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