Hydrogen Peroxide Vapor: A Novel Method for the Environmental Control of Lactococcal Bacteriophage†

Bacteriophage contamination can be problematic, especially in industrial settings. We examined the in vitro efficacy of hydrogen peroxide vapor (HPV) for the inactivation of two lactococcal bacteriophages dried onto stainless steel discs. A more than 6-log reduction was achieved on both bacteriophages compared with unexposed controls by 50 min of HPV exposure in an isolator. HPV might be useful for the environmental control of bacteriophages.

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A Head-to-Head Comparison of Hydrogen Peroxide Vapor and Aerosol Room Decontamination Systems

Infection Control and Hospital Epidemiology ◽

10.1086/661104 ◽

2011 ◽

Vol 32 (9) ◽

pp. 831-836 ◽

Cited By ~ 38

Author(s):

T. Holmdahl ◽

P. Lanbeck ◽

M. Wullt ◽

M. H. Walder

Keyword(s):

Hydrogen Peroxide ◽

Cycle Time ◽

New Technologies ◽

Biological Indicators ◽

The Other ◽

Test Room ◽

Cycle Times ◽

Hydrogen Peroxide Vapor ◽

In Vitro Comparison

Objective.New technologies have emerged in recent years for the disinfection of hospital rooms and equipment that may not be disinfected adequately using conventional methods. There are several hydrogen peroxide–based area decontamination technologies on the market, but no head-to-head studies have been performed.Design.We conducted a head-to-head in vitro comparison of a hydrogen peroxide vapor (HPV) system (Bioquell) and an aerosolized hydrogen peroxide (aHP) system (Sterinis).Setting.The tests were conducted in a purpose-built 136-m3test room.Methods.One HPV generator and 2 aHP machines were used, following recommendations of the manufacturers. Three repeated tests were performed for each system. The microbiological efficacy of the 2 systems was tested using 6-log Tyvek-pouchedGeobacillus stearo-thermophilusbiological indicators (BIs). The indicators were placed at 20 locations in the first test and 14 locations in the subsequent 2 tests for each system.Results.All BIs were inactivated for the 3 HPV tests, compared with only 10% in the first aHP test and 79% in the other 2 aHP tests. The peak hydrogen peroxide concentration was 338 ppm for HPV and 160 ppm for aHP. The total cycle time (including aeration) was 3 and 3.5 hours for the 3 HPV tests and the 3 aHP tests, respectively. Monitoring around the perimeter of the enclosure with a handheld sensor during tests of both systems did not identify leakage.Conclusion.One HPV generator was more effective than 2 aHP machines for the inactivation ofG. stearothermophilusBIs, and cycle times were faster for the HPV system.

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Hydrogen Peroxide Vapor Decontamination in a Patient Room Using Feline Calicivirus and Murine Norovirus as Surrogate Markers for Human Norovirus

Infection Control and Hospital Epidemiology ◽

10.1017/ice.2016.15 ◽

2016 ◽

Vol 37 (5) ◽

pp. 561-566 ◽

Cited By ~ 9

Author(s):

Torsten Holmdahl ◽

Mats Walder ◽

Nathalie Uzcátegui ◽

Inga Odenholt ◽

Peter Lanbeck ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Cell Culture ◽

Tissue Culture ◽

Feline Calicivirus ◽

Murine Norovirus ◽

Patient Room ◽

Human Norovirus ◽

Log Reduction ◽

Infective Dose ◽

Hydrogen Peroxide Vapor

OBJECTIVETo determine whether hydrogen peroxide vapor (HPV) could be used to decontaminate caliciviruses from surfaces in a patient room.DESIGNFeline calicivirus (FCV) and murine norovirus (MNV) were used as surrogate viability markers to mimic the noncultivable human norovirus. Cell culture supernatants of FCV and MNV were dried in triplicate 35-mm wells of 6-well plastic plates. These plates were placed in various positions in a nonoccupied patient room that was subsequently exposed to HPV. Control plates were positioned in a similar room but were never exposed to HPV.METHODSVirucidal activity was measured in cell culture by reduction in 50% tissue culture infective dose titer for FCV and by both 50% tissue culture infective dose titer and plaque reduction for MNV.RESULTSNeither viable FCV nor viable MNV could be detected in the test room after HPV treatment. At least 3.65 log reduction for FCV and at least 3.67 log reduction for MNV were found by 50% tissue culture infective dose. With plaque assay, measurable reduction for MNV was at least 2.85 log units.CONCLUSIONSThe successful inactivation of both surrogate viruses indicates that HPV could be a useful tool for surface decontamination of a patient room contaminated by norovirus. Hence nosocomial spread to subsequent patients can be avoided.Infect Control Hosp Epidemiol 2016;37:561–566

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Comparison of the Microbiological Efficacy of Hydrogen Peroxide Vapor and Ultraviolet Light Processes for Room Decontamination

Infection Control and Hospital Epidemiology ◽

10.1086/665326 ◽

2012 ◽

Vol 33 (5) ◽

pp. 507-512 ◽

Cited By ~ 57

Author(s):

Nancy L. Havill ◽

Brent A. Moore ◽

John M. Boyce

Keyword(s):

Hydrogen Peroxide ◽

Prospective Observational Study ◽

Biological Indicators ◽

Line Of Sight ◽

Geobacillus Stearothermophilus ◽

Log Reduction ◽

Before And After ◽

The Mean ◽

Mean Cycle ◽

Hydrogen Peroxide Vapor

Objective.To compare the microbiological efficacy of hydrogen peroxide vapor (HPV) and ultraviolet radiation (UVC) for room decontamination.Design.Prospective observational study.Setting.500-bed teaching hospital.Methods.HPV and UVC processes were performed in 15 patient rooms. Five high-touch sites were sampled before and after the processes and aerobic colony counts (ACCs) were determined. Carrier disks with ~106Clostridium difficile (CD) spores and biological indicators (BIs) with 104 and 106Geobacillus stearothermophilus spores were placed in 5 sites before decontamination. After decontamination, CD log reductions were determined and BIs were recorded as growth or no growth.Results.93% of ACC samples that had growth before HPV did not have growth after HPV, whereas 52% of sites that had growth before UVC did not have growth after UVC (P < .0001). The mean CD log reduction was >6 for HPV and ~2 for UVC. After HPV 100% of the 104 BIs did not grow, and 22% did not grow after UVC, with a range of 7%–53% for the 5 sites. For the 106 BIs, 99% did not grow after HPV and 0% did not grow after UVC. Sites out of direct line of sight were significantly more likely to show growth after UVC than after HPV. Mean cycle time was 153 (range, 140–177) min for HPV and 73 (range, 39–100) min for UVC (P < .0001).Conclusion.Both HPV and UVC reduce bacterial contamination, including spores, in patient rooms, but HPV is significantly more effective. UVC is significantly less effective for sites that are out of direct line of sight.

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Efficacy of commercially available disinfectants against human norovirus surrogates and Clostridioides difficile endospores

10.21203/rs.3.rs-903165/v1 ◽

2021 ◽

Author(s):

Jinge Huang ◽

Geun W Park ◽

Rachael Jones ◽

Angie Frazer ◽

Jan Vinje ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Stainless Steel ◽

Active Ingredient ◽

Strong Correlation ◽

Contact Time ◽

Human Norovirus ◽

Log Reduction ◽

Product Formulation ◽

Clostridioides Difficile ◽

Suspension Test

Abstract Background Routine disinfection of environmental surfaces is essential to control the spread of pathogens that cause healthcare-associated infections. The aim of this study was to determine the efficacy of a panel of disinfectants against two human norovirus surrogates [feline calicivirus (FCV) and Tulane virus (TuV)] and Clostridioides difficile endospores. Methods Nine products on EPA’s List G were selected using four criteria: 1) ready-to-use, 2) nonchlorine-based active ingredient, 3) commercially available, and 4) limited known health risks. Active ingredients of the products included hydrogen peroxide (H2O2), peracetic acid, quaternary ammonium compounds, or alcohols. The efficacy of the products against FCV, TuV and C. difficile spores was tested using the ASTM suspension test and on stainless steel coupons for 1, 5 and 10 min (FCV, TuV) and 10 min (C. difficile spores). Results On stainless steel carriers, seven of the nine products reduced FCV > 5.1 log after 5 min of contact time and eight products after 10 min. One product reduced TuV > 3.1 log after 5 min while 8 products could not achieve this level of reduction even after 10 min. Only one product achieved > 6.0 log reduction of C. difficile spores after 10 min while other 8 products could not achieve this acceptable efficacy. The two most efficacious products contained H2O2 as active ingredient and resulted in > 5.1 log reduction of FCV and ≥ 2.4 log reduction of TuV after 5 min contact time on stainless steel carriers. However, among the five products containing H2O2, no strong correlation (R2 = 0.25, p = 0.03) between log reduction and H2O2 concentration was detected using the ASTM suspension test, suggesting product formulation, not active ingredient concentration, impacts efficacy. Conclusion A panel of nine EPA-approved disinfectants was tested against two HuNoV surrogates (FCV and TuV) and C. difficile endospores. The most efficacious products contained hydrogen peroxide as active ingredient. TuV was confirmed to be the more conservative surrogate for HuNoV than FCV. No strong correlation between log reduction and hydrogen peroxide concentration was detected, suggesting that product formulation has a significant impact on the efficacy of a product.

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The nature of the oxide film formed on stainless steel immersed in concentrated hydrogen peroxide (H.T.P.)

Journal of Biochemical Toxicology ◽

10.1002/jbt.2570101205 ◽

1960 ◽

Vol 10 (12) ◽

pp. 497-505

Author(s):

J. Diederichsen

Keyword(s):

Hydrogen Peroxide ◽

Stainless Steel ◽

Oxide Film

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In vitro effects of hydrogen peroxide on ALF expression in male mouse germ cells

Reproduction Abstracts ◽

10.1530/repabs.1.p260 ◽

2014 ◽

Author(s):

Khaled S A Habas ◽

Diana Anderson ◽

Martin H Brinkworth

Keyword(s):

Hydrogen Peroxide ◽

Germ Cells ◽

Male Mouse ◽

In Vitro Effects

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Comparative Evaluation of Frictional forces between different Archwire-bracket Combinations

Orthodontic Journal of Nepal ◽

10.3126/ojn.v4i1.11307 ◽

2014 ◽

Vol 4 (1) ◽

pp. 22-28 ◽

Cited By ~ 1

Author(s):

Vinit Singh ◽

Swati Acharya ◽

Satyabrata Patnaik ◽

Smruti Bhusan Nanda

Keyword(s):

Stainless Steel ◽

Tooth Movement ◽

Testing Machine ◽

Frictional Resistance ◽

Nickel Titanium ◽

Orthodontic Bracket ◽

Fixed Appliance ◽

Beta Titanium ◽

Frictional Forces

Introduction: During sliding mechanics, frictional resistance is an important counterforce to orthodontic tooth movement; whichmust be controlled to allow application of light continuous forces.Objective: To investigate static and kinetic frictional resistance between three orthodontic brackets: ceramic, self-ligating, andstainless steel, and three 0.019×0.025” archwires: stainless steel, nickel-titanium, titanium-molybdenum.Materials & Method: The in vitro study compared the effects of stainless steel, nickel-titanium, and beta-titanium archwires onfrictional forces of three orthodontic bracket systems: ceramic, self-ligating, and stainless steel brackets. All brackets had 0.022”slots, and the wires were 0.019×0.025”. Friction was evaluated in a simulated half-arch fixed appliance on a testing machine. Thestatic and kinetic friction data were analyzed with 1-way analysis of variance (ANOVA) and post-hoc Duncan multiple rangetest.Result: Self-ligating (Damon) brackets generated significantly lower static and kinetic frictional forces than stainless steel (Gemini)and ceramic brackets (Clarity). Among the archwire materials, Beta-titanium showed the maximum amount of frictional forceand stainless steel archwires had the lowest frictional force.Conclusion: The static and kinetic frictional force for stainless steel bracket was lowest in every combination of wire.

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Efficient and selective catalytic hydroxylation of unsaturated plant oils: a novel method for producing anti-pathogens

BMC Chemistry ◽

10.1186/s13065-021-00748-z ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

Ahmed M. Senan ◽

Binru Yin ◽

Yaoyao Zhang ◽

Mustapha M. Nasiru ◽

Yong‐Mei Lyu ◽

...

Keyword(s):

Antimicrobial Agents ◽

In Vitro Study ◽

Functional Method ◽

Plant Oils ◽

Main Process ◽

Catalytic Method ◽

Novel Method ◽

Pharmaceutical Industries ◽

Increasing Demand

AbstractWith the increasing demand for antimicrobial agents and the spread of antibiotic resistance in pathogens, the exploitation of plant oils to partly replace antibiotic emerges as an important source of fine chemicals, functional food utility and pharmaceutical industries. This work introduces a novel catalytic method of plant oils hydroxylation by Fe(III) citrate monohydrate (Fe3+-cit.)/Na2S2O8 catalyst. Methyl (9Z,12Z)-octadecadienoate (ML) was selected as an example of vegetable oils hydroxylation to its hydroxy-conjugated derivatives (CHML) in the presence of a new complex of Fe(II)-species. Methyl 9,12-di-hydroxyoctadecanoate 1, methyl-9-hydroxyoctadecanoate 2 and methyl (10E,12E)-octadecanoate 3 mixtures is produced under optimized condition with oxygen balloon. The specific hydroxylation activity was lower in the case of using Na2S2O8 alone as a catalyst. A chemical reaction has shown the main process converted of plantoils hydroxylation and (+ 16 Da) of OH- attached at the methyl linoleate (ML-OH). HPLC and MALDI-ToF-mass spectrometry were employed for determining the obtained products. It was found that adding oxidizing agents (Na2S2O8) to Fe3+ in the MeCN mixture with H2O would generate the new complex of Fe(II)-species, which improves the C-H activation. Hence, the present study demonstrated a new functional method for better usage of vegetable oils.Producing conjugated hydroxy-fatty acids/esters with better antipathogenic properties. CHML used in food industry, It has a potential pathway to food safety and packaging process with good advantages, fundamental to microbial resistance. Lastly, our findings showed that biological monitoring of CHML-minimum inhibitory concentration (MIC) inhibited growth of various gram-positive and gram-negative bacteria in vitro study. The produced CHML profiles were comparable to the corresponding to previousstudies and showed improved the inhibition efficiency over the respective kanamycin derivatives.

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