scholarly journals Analysis of N2O emissions and isotopomers to understand nitrogen cycling associated with multispecies grassland swards at a lysimeter scale

Author(s):  
Conor Bracken ◽  
Gary Lanigan ◽  
Karl Richards ◽  
Saoirse Tracy ◽  
Christoph Müller ◽  
...  

<p>Nitrous oxide (N<sub>2</sub>O) is a potent greenhouse gas associated with nitrogen fertiliser inputs to agricultural production systems. Minimising N<sub>2</sub>O emissions is important to improving the efficiency and sustainability of grassland agriculture. Multispecies grassland swards composed of plants from different functional groups (grasses, legumes, herbs) have been considered as a management strategy to achieve this goal. Numerous soil nitrogen transformation pathways can lead to the production of N<sub>2</sub>O emissions. These transformation pathways are regulated by soil microbial communities and the environmental conditions and management practices that impact on them. Much research has been carried out on N cycling and N<sub>2</sub>O emissions from predominantly grass monoculture systems. However, there is a lot yet to understand about how agricultural grasslands with diverse plant communities influence soil N cycling and N<sub>2</sub>O emissions. A lysimeter experiment was set up as a completely randomised block design and carried out over a full year to investigate N<sub>2</sub>O production, and nitrogen cycling associated with four sward types. The swards four swards were: perennial ryegrass (PRG, Lolium perenne); PRG and low white clover (PRG + LWC, Trifolium repens); PRG and high white clover (PRG + HWC); PRG, WC and ribwort plantain (PRG + WC + PLAN, Plantago lanceolata) managed at 250, 90, 0, and 45 kg N ha<sup>-1</sup>yr<sup>-1</sup>, respectively. Fertiliser N was applied by syringe as urea in splits at suitable timings to meet grass growth demands. N<sub>2</sub>O fluxes were measured using a static chamber technique and additional samples were taken after the final flux sample to measure the associated N<sub>2</sub>O isotopomers using a novel Cavity Ring Down Spectroscopy technique. Leachate volumes were measured on a weekly basis and composite monthly samples were used to determine the total amount of N leached from each treatment over the full year. Herbage was harvested on a monthly basis to measure DM yield (kg DM ha<sup>-1</sup>), total N (%) and N yield (kg N ha<sup>-1</sup>).This work reports on the N<sub>2</sub>O emissions and N leaching associated with the four sward treatments and related these N losses to the treatments DM yields and N uptake as an estimation of the efficiency of these differing grassland management strategies. N<sub>2</sub>O isotopomer measurements were used to indicate N transformation pathways driving N loss over the growing season particularly around periods of peak N<sub>2</sub>O emissions.</p>

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Khadim Dawar ◽  
Shah Fahad ◽  
M. M. R. Jahangir ◽  
Iqbal Munir ◽  
Syed Sartaj Alam ◽  
...  

AbstractIn this study, we explored the role of biochar (BC) and/or urease inhibitor (UI) in mitigating ammonia (NH3) and nitrous oxide (N2O) discharge from urea fertilized wheat cultivated fields in Pakistan (34.01°N, 71.71°E). The experiment included five treatments [control, urea (150 kg N ha−1), BC (10 Mg ha−1), urea + BC and urea + BC + UI (1 L ton−1)], which were all repeated four times and were carried out in a randomized complete block design. Urea supplementation along with BC and BC + UI reduced soil NH3 emissions by 27% and 69%, respectively, compared to sole urea application. Nitrous oxide emissions from urea fertilized plots were also reduced by 24% and 53% applying BC and BC + UI, respectively, compared to urea alone. Application of BC with urea improved the grain yield, shoot biomass, and total N uptake of wheat by 13%, 24%, and 12%, respectively, compared to urea alone. Moreover, UI further promoted biomass and grain yield, and N assimilation in wheat by 38%, 22% and 27%, respectively, over sole urea application. In conclusion, application of BC and/or UI can mitigate NH3 and N2O emissions from urea fertilized soil, improve N use efficiency (NUE) and overall crop productivity.


Nitrogen ◽  
2020 ◽  
Vol 1 (2) ◽  
pp. 167-189
Author(s):  
Daniel M. Alongi

Nitrogen (N) cycling in mangroves is complex, with rapid turnover of low dissolved N concentrations, but slow turnover of particulate N. Most N is stored in soils. The largest sources of N are nearly equal amounts of mangrove and benthic microalgal primary production. Dissolved N fluxes between the forests and tidal waters show net uptake, indicating N conservation. N2-fixation is underestimated as rapid rates measured on tree stems, aboveground roots and cyanobacterial mats cannot currently be accounted for at the whole-forest scale due to their extreme patchiness and the inability to extrapolate beyond a localized area. Net immobilization of NH4+ is the largest ecosystem flux, indicating N retention. Denitrification is the largest loss of N, equating to 35% of total N input. Burial equates to about 29% of total inputs and is the second largest loss of N. Total inputs slightly exceed total outputs, currently suggesting net N balance in mangroves. Mangrove PON export equates to ≈95% of PON export from the world’s tropical rivers, but only 1.5% of the entire world’s river discharge. Mangrove N2O emissions, denitrification, and burial contribute 0.4%, 0.5–2.0% and 6%, respectively, to the global coastal ocean, which are disproportionate to their small worldwide area.


SoilREns ◽  
2018 ◽  
Vol 16 (1) ◽  
Author(s):  
Anni Yuniarti ◽  
Yuliati Machfud ◽  
Eso Solihin ◽  
Yogi Sudirman ◽  
Apong Sandrawati

Inceptisol is a widespread soil order with low fertility. Hence it is necessary to improve its quality status through the application of chemical and biological fertilizers. The main objecttive of this study is to determine the effect of NPK fertilizer and biofertlizer consortia (BC) combinations in Inceptisols towards N and P availability in soil and uptake by soybean (Glycine max L.). Experiments were carried out from April to August 2016 at Ciparanje Experimental Farm, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor, Sumedang at the approximate altitude of 720 meters above the sea level. Randomized Block Design experimental design consisted of ten treatments and three replications was used in this study. Treatments consisted of control (no treatment), Recommended NPK dosage, 0 NPK + 1 BC, ¼ NPK + 1 BC, ½ NPK + 1 BC, ¾ NPK + 1 BC, 1 NPK + 1 BC, ¾ NPK + ¼ BC, ¾ NPK + ½ BC and also ¾ NPK + ¾ BC. Experimental results showed that the combination of NPK fertilizer and soybean-spesific biofertilizer consortium increased soil total phosphate content,  nitrogen uptake by plant, and soyben yield significantly.  Keywords: Biofertilizer, Soybean, total-N, Uptake of N, Inceptisols


2019 ◽  
Vol 5 (02) ◽  
pp. 242-248
Author(s):  
V. Namdeo ◽  
N. G. Mitra ◽  
S. R. Jakhar ◽  
R. K. Sahu

Field experiments were conducted to Influence of different levels of nitrogen and Azospirillum inoculation on direct-seeded rice in a Vertisol, during kharif season of 2015 at Department of Soil Science and Agricultural Chemistry, JNKVV, Jabalpur. The experiment was laid out under randomized block design (RBD) with 3 replications and 8 treatments namely (unfertilized+uninoculated (UFUI), recommended dose of nitrogen 50%+uninoculated (RDN50%+UI), RDN75%+UI, RDN100%+UI, UF+Azospirillum (UF+Azosp.,), RDN50%+Azosp., RDN75%+Azosp and RDN100%+Azosp.,). It was observed that significant improvement was noticed in yield attributes and soil properties. The response from the treatment of RDN100% +Azosp., was found statistically best to increase available nitrogen (N) content in soil at 45 DAS and at harvest of the crop by 29% and 27%, respectively and N content in the plant, grain and straw by 46%, 50% and 55%, respectively over the control of UFUI. Similarly, trend was significantly enhanced total N uptake by crop with 129% over the control of UFUI. While, same treatment combination increasing azospiral population in rhizospheric soil at 45 DAS, 65 DAS and at harvest by 2.28, 2.07 and 2.05 log folds, respectively over the control of UFUI and enhanced yield attributes and yields of grain and straw of rice with 113 and 58%, respectively over the control of UFUI. While the treatment RDN100%+Azosp., exhibited numerically higher values but was statistically at par to RDN75%+Azosp.


2015 ◽  
Vol 39 (1) ◽  
pp. 127-139 ◽  
Author(s):  
Marco André Grohskopf ◽  
Paulo Cezar Cassol ◽  
Juliano Corulli Correa ◽  
Maria Sueli Heberle Mafra ◽  
Jonas Panisson

The application of pig slurry may have a different effect on nitrogen dynamics in soil compared to mineral fertilization. Thus, the aim of this study was to determine the different forms of organic N in a Latossolo Vermelho distroférrico (Typic Hapludox) and their relationship to N uptake by crops in response to 10 years of annual application of pig slurry and mineral fertilizer. The treatments were application rates of 0, 25, 50, 100, and 200 m3 ha-1 of pig slurry, in addition to mineral fertilizer, organized in a randomized block design with four replications. The N contents were determined in the plant tissue and in the forms of total N and acid hydrolyzed fractions: ammonium-N, hexosamine-N, α-amino-N, amide-N, and unidentified-N. Annual application of pig slurry or mineral fertilizer increased the total-N content in the 0-10 cm depth layer. The main fractions of organic N in the soil were α-amino-N when pig slurry was applied and unidentified-N in the case of mineral fertilizers. Pig slurry increased the N fractions considered as labile: α-amino-N, ammonium-N, and amide-N. The increase in these labile organic N fractions in the soil through pig slurry application allows greater N uptake by the maize and oat crops in a no-tillage system.


SoilREns ◽  
2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Shindy Widiana ◽  
Anni Yunarti ◽  
Emma Trinurani Sofyan ◽  
Dirga Sapta Sara

Shallot (Allium ascalonicum L.) is a horticulture commodity that has a lot of benefits and also prospective due its increasing demand. Annual shallot productivity in Indonesia is unstable due to nutrient degradation on agricultural land, therefore efforts are needed to promote the productivity of shallot. One of the solution using NPK fertilization. This experiment aimed to determine the effect of NPK compound fertilizer on N total, N-uptake, and shallot bulb yield. This research conducted at the Soil Chemical and Plant Nutrition Experiments Field, Faculty of Agriculture, Padjadjaran University, Jatinangor, from February to June 2019. The experimental design used was Randomized Block Design (RBD) consisted of one control treatment, one N, P, K single fertilizer treatment (250 kg ha-1 Urea, 125 kg ha-1 ZA, 200 kg ha-1 SP-36, and 250 kg ha-1 KCl), and eight NPK compound fertilizer treatments in various doses with three replications. The results showed that the NPK compound treatment affected the total N, N uptake, and shallot bulbs yield. Treatment of 75% NPK compound (675 kg ha-1) gave the highest results of fresh bulbs weight at 41.64 g/clump and dry bulbs weight at 25.27 g/clump.


2019 ◽  
Vol 99 (2) ◽  
pp. 173-181
Author(s):  
Mehdi Sharifi ◽  
Scott Baker ◽  
Leila Hojabri ◽  
Monireh Hajiaghaei-Kamrani

The co-product of anaerobic digestion, digestate, is nitrogen (N) rich; however, the forms and accessibility of this N by the crops have not been fully explored. This study aimed to determine the mineralization parameters of digestate N and to assess its availability for annual ryegrass (Lolium multiflorum Lam.). Four digestate rates of 0 (control), 38, 75, and 150 mg N kg−1 soil (equal to 0, 90, 180, and 360 kg total N ha−1) were applied to a silty clay loam soil in a completely randomized block design with four replications in a greenhouse study. A 100 d aerobic incubation experiment was also conducted with 0 and 150 mg digestate N kg−1 rates at 25 °C. Digestate feedstock included cattle manure (28%), hay (15%), and silage corn (Zea mays L.; 57%). Total plant biomass and N uptake increased linearly with digestate application rate with average apparent N recovery of 37%. Potentially mineralizable N (N0) and mineralizable N rate constant (k) were not significantly different in digestate and control treatments; however, a flush of digestate organic N (30 mg N kg−1) released right after mixing the digestate with soil. Evidences of N immobilization with digestate application were observed in greenhouse study. Majority of plant-available digestate N was in form of NH4+-N; therefore, NH4+-N can be used for estimation of available digestate N for crops. Results need to be validated for specific feedstock and soil properties under field conditions. Further research is needed to assess how long-term build-up of digestate organic N may impact the N availability for crops.


Author(s):  
Kamran Azeem ◽  
Farah Naz ◽  
Arshad Jalal ◽  
Fernando S. Galindo ◽  
Marcelo C. M. Teixeira Filho ◽  
...  

ABSTRACT Humic acid (HA), as a bio-stimulant and a major component of organic matter (OM), can improve plant physiology, soil fertility, and nutrient availability, mainly in low OM soils. Nitrogen (N) is one of the most important nutrients that affect several metabolic and biochemical activities, leading to improved plant development. This study was conducted to investigate the combined effect of HA and N doses on soil organic matter (SOM) and total N concentration, N uptake, corn growth, and grain yield under conventional tillage at Peshawar, Pakistan. Treatments were tested in a randomized block design with four replicates arranged in a factorial scheme 3 × 4 + 1. The respective doses of HA (1.5, 3,0 and 4.5 kg ha-1) were applied at the corn sowing, whereas N doses (80, 120, 160, and 200 kg ha-1) were applied in three splits (1/3 at sowing, 1/3 at the V5 stage, and remaining 1/3 at the tasselling stage) with one control (no HA and N). The application of HA, regardless of the applied doses, had positive effects on SOM, N concentration, N uptake, corn development, and grain yield. However, the application of 4.5 kg ha-1 of HA was the most effective in promoting SOM (0.83%) and total N (0.31%), shoot biomass (10610 kg ha-1), N uptake (1.13%), and grain yield (3780 kg ha-1), even when combined with the N doses of 80, 120 and 160 kg N ha-1. Increasing N doses positively influenced SOM, N concentration, N uptake, and corn growth. The greatest grain yield was obtained at 150 kg ha-1 of N regardless of HA applied doses.


SoilREns ◽  
2020 ◽  
Vol 17 (2) ◽  
Author(s):  
Rija Sudirja ◽  
Maya Damayani ◽  
Eso Solihin ◽  
Wulan Sri Damayanti

Rice is one of the staples of Indonesian society. Development of rice cultivation can be carried out on Inceptisol soils. This land has a wide distribution of around 70.52 million, but it has an unfavorable fertility rate so fertilization is needed in order to increase fertility and yield of lowland rice. This study aims to determine the effect of a combination of liquid organic fertilizer and N, P, K fertilizer on C-Organic, N-Total, N uptake and Yield (Oryza Sativa L.) on Inceptisol from Jatinangor. The experiment was held on March 2019 until July 2019 at Ciparanje Experimental Field and Laboratory Soil Chemistry and Plant Nutrition, Faculty of Agriculture, Padjadjran University, Jatinangor, Sumedang. The experimental design was Randomized Block Design with ten treatments and three replications. The result of study showed that the combination of liquid organic fertilizer and N, P, K is affected on organic C, total N, N uptake and rice yield. The application of one liquid organik fertilizer and ¾ N, P, K gave the best rice yield of 8,55 kg/plot or equal to 6,84 ton/ha.


2015 ◽  
Vol 6 (2) ◽  
pp. 115-120
Author(s):  
MM Ali ◽  
MH Rahman ◽  
MR Khan ◽  
MK Khan

Four field experiments were carried out with Binadhan 7 at Birgonj, Dinajpur; Debigonj, Panchagarh; Kaligonj, Lalmonirhat and Pirgonj, Rangpur during T.aman (kharif II) season of 2011 in the Old Himalalayan Piedmont Plain (AEZ 1) and Tista Meander Floodplain Soils of North-West Bangladesh. The experiments were designed with eight treatments and laid out in randomized complete block design (RCBD) with three replications. The treatment combinations were: T1 (100%STB), T2 (T1 + 25% N), T3 (T1 + 25% NP), T4 (T1 + 25% NK), T5 (T1 + 25% PK), T6 (T1 + 25% NPK), T7 (75% of T1) and T8 (Control). Results indicated that application of different fertilizers significantly affected the grain yield at all of the four locations. The highest grain yield was found in treatment T6 (T1 + 25% NPK). Statistically identical yield was observed in treatments T3, T4 and T5 at Dinajpur; T3 at Panchagar; T2, T3, and T4 at Lalmonirhat and T2 at Rangpur. All the treatment combinations gave significantly higher grain yield over the control at all of the locations. The highest straw yield was found in treatment T6 (T1 + 25% NPK). Statistically identical straw yield was found in T3 and T4 at Dinajpur; T1, T4 and T5 at Panchagar; T2, T4, T5 and T7 Lalmonirhat and all treatments except T7 and T8 at Rangpur. Significantly the lowest yield was obtained in the control treatment (T8) in all of the locations. The highest total N uptake was observed in treatment T6 at all of the four locations. For total P uptake, the similar trend of total N uptake was observed for all the locations. Treatment T6 showed the highest uptake of total K at all locations. The highest uptake of total S was observed in treatment T6 at Dinajpur, Panchagarh and Lalmonirhat. But the highest uptake of total S was observed in treatment T3 at Rangpur. However, the lowest total N, P, K and S uptake was observed in control treatment (T8) at all locations. The partial budget analysis of T.aman rice demonstrated the highest net benefit of tkha-1 85,598/-, 78,619/-, 58,308/- and 72,532/- in T6 treatment followed by tkha-1 76,348/- in T4 treatment, 71,100/- in T3 treatment, 54,192/- in T3 treatment and 68,247/- in T2 treatment where the highest MBCR of 5.40, 3.75, 2.20 and 3.84 was also observed in T6 treatment at Dinajpur, Panchagarh, Lalmonirhat and Rangpur, respectively. Based on most profitable treatments, fertilizer doses of N80P8K40S8Zn1 in Birgonj, Dinajpur; N84P8K40S6Zn1 in Debigonj, Panchagarh; N80P8K40S6Zn1 in Kaligonj, Lalmonirhat and N81P8K35S8Zn1 in Pirgonj, Rangpur could be recommended for higher yield of Binadhan-7 rice in North-West Bangladesh.DOI: http://dx.doi.org/10.3329/jesnr.v6i2.22106 J. Environ. Sci. & Natural Resources, 6(2): 115-120 2013


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