scholarly journals Comparison of MALDI-TOF MS Method Using Samples From Blood Culture Bottles and Germ Tube Test in Rapid Detection and Identification of Candidemia

2021 ◽  
Author(s):  
Kerem Yılmaz ◽  
Nurten Altanlar
Keyword(s):  
Blood Culture ◽  
Rapid Detection ◽  
Germ Tube ◽  
Maldi Tof Ms ◽  
Tube Test ◽  
Maldi Tof ◽  
Detection And Identification ◽  
Tof Ms

Rapid detection and identification of strains carrying carbapenemases directly from positive blood cultures using MALDI-TOF MS

2014 ◽  
Vol 105 ◽  
pp. 98-101 ◽  
Author(s):  
Y. Hoyos-Mallecot ◽  
C. Riazzo ◽  
C. Miranda-Casas ◽  
M.D. Rojo-Martín ◽  
J. Gutiérrez-Fernández ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Blood Cultures ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Detection And Identification ◽  
Tof Ms

scholarly journals Rapid Identification of Microorganisms from Positive Blood Culture by MALDI-TOF MS After Short-Term Incubation on Solid Medium

2016 ◽  
Vol 74 (1) ◽  
pp. 97-102 ◽  
Author(s):  
Antonio Curtoni ◽  
Raffaella Cipriani ◽  
Elisa Simona Marra ◽  
Anna Maria Barbui ◽  
Rossana Cavallo ◽  
...  
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Solid Medium ◽  
Rapid Identification ◽  
Short Term ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

S1. A modified method for blood culture direct testing using 2% SDS detergent and heat. v1

2021 ◽  
Author(s):  
kwenrich not provided
Keyword(s):  
Blood Culture ◽  
Clinical Laboratory ◽  
Sodium Dodecyl ◽  
Agar Plate ◽  
Drying Methods ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Modified Method ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) can accurately identify bloodstream pathogens directly from positive blood culture bottles without the need to wait for agar plate growth. In this study, 2% sodium dodecyl sulfate (SDS) detergent was assessed to determine its benefit in the removal of interfering cellular components for testing on the Bruker Microflex LT MALDI-TOF MS instrument with the Biotyper® CA system. Additionally, the use of a heat-drying step was evaluated for performance improvement over conventional air-drying of samples on the MALDI steel target plate. The modified method with 2% SDS outperformed the in-house protocol in overall success with percentage scores of 91% and 55% ( respectively). The data results support the potential of applying a simple lysing step to an existing in-house extraction method and the use of modified drying methods. The modified techniques evaluated in this study proved beneficial for identifying most blood culture pathogens encountered in the clinical laboratory, and they can allow for reduced turnaround times and more appropriate antibiotic treatments.

scholarly journals Stable isotope labelling: an approach for MALDI-TOF MS-based rapid detection of fluconazole resistance in Candida tropicalis

2019 ◽  
Vol 74 (5) ◽  
pp. 1269-1276 ◽  
Author(s):  
Saikat Paul ◽  
Shreya Singh ◽  
Arunaloke Chakrabarti ◽  
Shivaprakash M Rudramurthy ◽  
Anup K Ghosh
Keyword(s):  
Stable Isotope ◽  
Candida Tropicalis ◽  
Rapid Detection ◽  
Stable Isotope Labelling ◽  
Fluconazole Resistance ◽  
Isotope Labelling ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Rapid identification of bacteria from bioMerieux BacT/ALERT blood culture bottles by MALDI-TOF MS

2013 ◽  
Vol 70 (4) ◽  
pp. 149-155 ◽  
Author(s):  
J.D. Haigh ◽  
I.M. Green ◽  
D. Ball ◽  
M. Eydmann ◽  
M. Millar ◽  
...  
Keyword(s):  
Blood Culture ◽  
Rapid Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Identification Of Bacteria ◽  
Tof Ms
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