scholarly journals Effects of dietary fats with different n-3 polyunsaturated fatty acid and n-6 polyunsaturated fatty acid ratios on the fatty acid compositions of serum and liver lipid fractions, and serum lipoprotein fractions in rats.

1990 ◽  
Vol 48 (4) ◽  
pp. 165-176 ◽  
Author(s):  
Toshichika Takita ◽  
Kahoru Nakamura ◽  
Takashi Hayakawa ◽  
Kaoru Suzuki ◽  
Satoshi Innami
Keyword(s):  
Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
Liver Lipid ◽  
Serum Lipoprotein ◽  
Dietary Fats ◽  
Lipid Fractions ◽  
Fatty Acid Compositions

Determination of Total Lipid and Lipid Subclasses in Meat and Meat Products

1987 ◽  
Vol 70 (1) ◽  
pp. 74-77
Author(s):  
Robert J Maxwell
Keyword(s):  
Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
Analytical Methods ◽  
Lipid Extraction ◽  
Meat Products ◽  
Food Material ◽  
Total Recovery ◽  
Nonpolar Solvents ◽  
Lipid Fractions ◽  
Nonpolar Lipids

Abstract Current interest in physiological and nutritional activities of the sterol, polyunsaturated fatty acid, and polar lipid fractions of meats and other foods indicates that analytical methods for lipids should be evaluated on their ability to recover and quantitate these classes. Current methods of lipid isolation furnish an extract that is dependent on the solvent(s) used, the type of food material, the temperature of extraction, and the relative proportions of the lipid classes present. Extraction with ethers or other relatively nonpolar solvents removes principally the neutral fats and nonpolar lipids. For an approximation of the crude fat content, such extraction is often sufficient, because the nonpolar fraction generally constitutes over 90% of the total lipids present. The polar lipids include the biochemically important (ω-3) and (ω-6) polyunsaturated fatty acid classes; thus, the method of lipid extraction of food products becomes relevant for a more complete and valuable characterization of their nutritional value. The various methods of lipid determination for meat products are examined for their total recovery of these important lipid groups. A sequential extraction in conjunction with subsequent analytical methods is recommended.

Variations in the fatty acid compositions of the liver and gonad tissue of spiny eel (Mastacembelus mastacembelus) from Atatürk Dam Lake

2017 ◽  
Vol 42 (6) ◽  
Author(s):  
Semra Kaçar ◽  
Mehmet Başhan
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Liver Tissue ◽  
Methyl Esters ◽  
Saturated Fatty Acids ◽  
Liver Lipid ◽  
Docosapentaenoic Acid ◽  
Atatürk Dam Lake ◽  
Chloroform Methanol ◽  
Fatty Acid Compositions

AbstractObjective:The aim of the study was to investigate quantitative and qualitative fatty acid profiles of the gonad and liver tissue of femaleMethods:Total lipids were extracted with 5 mL of chloroform-methanol (2:1 v/v). Samples containing gonad and liver lipid were transesterified with acidified methanol. The fatty acid methyl esters were extracted with hexane. Fatty acids were detected by gas chromatography (GC).Results:The major saturated fatty acids (SFAs) were myristic acid (C14:0), palmitic acid (C16:0) and stearic acid (C18:0) in both gonad and liver tissue. Oleic acid (C18:1 ω-9) and palmitoleic acid (C16:1 ω-7) were the prominent monounsaturated fatty acid (MUFA). The dominant polyunsaturated fatty acids (PUFAs) were linoleic acid (LA, C18:2 ω-6), arachidonic acid (AA, C20:4 ω-6), docosapentaenoic acid (C22:5 ω-3) and docoesahexaenoic acid (DHA, C22:6 ω-3). The ratio of ω-3/ω-6 ranged from 1.55 to 3.44 and 1.18 to 2.71 in the gonad and liver tissue, respectively.Conclusion:The findings of this study will be useful for understanding the seasonal distribution of fatty acid composition in the liver and gonad of spiny eel.

THE INFLUENCE OF DIFFERENT DIETARY FATS ON THE REPRODUCTIVE PERFORMANCE OF TURKEY HENS

1990 ◽  
Vol 70 (2) ◽  
pp. 679-684 ◽  
Author(s):  
C. VILCHEZ ◽  
S. P. TOUCHBURN ◽  
E. R. CHAVEZ ◽  
C. W. CHAN
Keyword(s):  
Fatty Acid ◽  
Olive Oil ◽  
Dietary Fat ◽  
Egg Production ◽  
Corn Oil ◽  
Dietary Fats ◽  
Breeder Hens ◽  
Fat Composition ◽  
Feeding Diets ◽  
Fatty Acid Compositions

Turkey breeder hens of the Quebec line were fed diets with no added fat, 3.5% animal-vegetable fat blend (A-V fat), or 3.5% fat provided by 4.4% dry fat product (DF), from 30 to 49 wk of age. The addition of A-V fat and DF to the breeder diets significantly (P < 0.05) reduced embryonic mortality between 8 and 28 d of incubation but had no effect on other reproductive parameters. Feeding diets with 0 or 5% A-V fat in a prebreeder diet from 21 to 28 wk of age in another experiment led to a detectable decrease in subsequent egg production. Those diets were followed by breeder diets with either no added fat, 5% A-V fat, 5% corn oil, or 5% olive oil from 28 to 48 wk of age. Hens fed no added dietary fat exhibited significantly higher fertility while those fed 5% corn oil laid the heaviest eggs. Hens fed 5% olive oil exhibited significantly higher embryo livability from 8 to 28 d of incubation and the highest hatchability of fertile eggs. Fatty acid compositions of plasma and yolk lipids were affected by the dietary fat composition. Key words: Turkey hens, dietary fat, reproduction, fatty acid

A Simple Quantitative Procedure for the Partition of Free and Esterified FattyAcids from Fecal Lipids

1971 ◽  
Vol 49 (5) ◽  
pp. 487-492 ◽  
Author(s):  
R. M. G. Hamilton ◽  
B. E. McDonald
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Coconut Oil ◽  
Dietary Fats ◽  
Gas Liquid Chromatography ◽  
Lipid Mixtures ◽  
Lipid Fractions ◽  
Subsequent Determination ◽  
Layer Chromatography

A procedure is presented for the partition of dietary and fecal lipids into free fatty acids (FFA), esterified fatty acids (EFA), and nonsaponifiable (NSF) fractions. The method permits quantitation of the lipid fractions and subsequent determination of the fatty acid composition of the FFA and EFA fraction by gas–liquid chromatography (g.l.c). Petroleum ether soluble lipids of acidified (HCl–ethanol) lyophilized feces and feed were partitioned into FFA and an esterified fatty acid plus nonsaponifiable fraction using a 0.05 N KOH – 60% ethanol partitioning solvent. The latter fraction was saponified and partitioned into EFA and NSF. Total recoveries of 93–100% were obtained with the method for lipid mixtures of known composition, dietary fats except coconut oil (88%), and fecal lipids. Separation of the lipids into individual fractions by this method was shown to be essentially complete by thin-layer chromatography and g.l.c. analysis.

Sign in / Sign up

Export Citation Format

Share Document