Necrotizing Enter colitis (NEC) is the devastating disease that affects mostly the intestine of premature infants. The wall of the intestine is invaded by bacteria, which cause local infection and inflammation that can ultimately destroy the wall of the wall of the bowel. NEC can develop in any new born within 2 weeks after birth. However it is most common in pre mature infants.NEC can be staged into three groups, to guide appropriate treatment based on the work of Bell et al. . In general, stage I and II are managed medically whereas stage III is managed surgically.Trigger factors (i.e. prenatal hypoxia, mild infection or formula feeding) cause focal mild intestinal mucosal injury. In the presence of proliferation of commensal bacteria, local breakdown of mucosal barrier may cause entry of bacterial products (e.g. lipo polysaccharides, platelet-activating factor). Aim: The aim of the study was to assess the knowledge regarding necrotizing enterocolitis Method And Materials: A pre experimental study used to accomplish the objectives. Study was undertaken on 60 primigravida mothers in pediatric ward in Shri Mahant Indresh Hospital Dehradun by using convenient sampling technique. Participants were selected on the basis of inclusion and exclusion criteria Structure knowledge questionnaire , observation , pre test , post test were used to asses knowledge and practice. Conceptual Framework: Conceptual framework acts as a building blocks for study. In this study , modified General system theory was used as the investigator aim as to assess the pre test knowledge and post test knowledge regarding necrotizing enterocolitis. Result:There is highest percentage of primigravida mothers according to their Table and Graph shows the Age interval wise distribution of the study subjects. It is illustrated from table that the majority of the participant 26(43.3%) in were 26-30 year compare to another age interval in our study. The Area of Residence wise distribution of the study subjects. It is illustrated from table that the majority of the participant 26(43.3%) in were Rural compare to another Area of Residence in our study. The Monthly Income of Family wise distribution of the study subjects. It is illustrated from table that the majority of the participant 21(35.0%) in were >40000 compare to another Monthly Income of Family in our study. The Occupation wise distribution of the study subjects. It is illustrated from table that the majority of the participant 25(41.7%) in were Gov employee compare to another Occupation in our study. The First Language wise distribution of the study subjects. It is illustrated from table that the majority of the participant 49(81.7%) in were the Religion wise distribution of the study subjects. It is illustrated from table that the majority of the participant 49(81.7%) in were Hindu compare to another Religion in our study. the Education wise distribution of the study subjects. It is illustrated from table that the majority of the participant 23(38.3%) in were High School compare to another Education in our study. the Type of Family wise distribution of the study subjects. It is illustrated from table that the majority of the participant 40(66.7%) in were Nuclear compare to another Type of Family in our study. Table and Graph shows the Mode of Delivery wise distribution of the study subjects. It is illustrated from table that the majority of the participant 37(61.7%) in were Normal compare to another Mode Of Delivery in our study. the Pre Test Level wise distribution of the study subjects. It is illustrated from table that the majority of the participant 55(91.7%) in were Inadequate Knowledge compare to another Pre Test Level in our study. There is significant difference between the pre-test and post test score of knowledge questionnaires was demonstrated by using paired test and computed between pre test and post test knowledge score. The value of paired test of knowledge score is 15.20 with the table value of 2.02 at 0.05 level of significance was found to be highly significant relationship between pre test and post test scores .So, in this study it was found that Structural teaching program was effective in improving the knowledge level among the primigravida mothers regarding necrotizing enter colitis. The study proved that there was no association found between knowledge score and selected demographic variables of age of primigravida mothers, Area of residence , Monthly income of family , Occupation , First language , Religion , Education , Socio economic status , Type of family , Mode of delivery at 0.05 level of significance. Hence the research hypothesis H2 is not accepted. The study proved that there is no significant association between knowledge scores with their selected demographic variables. Discussion:Based on the objectives of the study, a knowledge questionnaire was used to assess the effectiveness of structured teaching program. It was considered to be appropriate instrument. Conclusion: Study revealed that in response to knowledge questionnaires in Pre test of subject 0% have adequate knowledge and that was increased to 55%. Moderate knowledge score in pre test was 5% that was reduced to 40%. Inadequate knowledge score in pre test was 8.3% that was to 0% in post test. This indicate that Structural teaching program are effective in improving knowledge.