scholarly journals Effective Purification of 1,4-.BETA.-Endoglucanase (EG66) from a Marine Mollusc, Patinopecten yessoensis, by Cellulose Column Chromatography

2009 ◽  
Vol 56 (1) ◽  
pp. 13-16
Author(s):  
Ken Sakai ◽  
Kyohei Ohtaki ◽  
Hideyo Koizumi ◽  
Shota Sato ◽  
Hisaoki Suzuki ◽  
...  
Keyword(s):  
Column Chromatography ◽  
Patinopecten Yessoensis ◽  
Marine Mollusc ◽  
Cellulose Column

A simple assay for galactokinase using deae-cellulose column chromatography

1977 ◽  
Vol 74 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Y.S. Shin-Buehring ◽  
M. Osang ◽  
R. Ziegler ◽  
J. Schaub
Keyword(s):  
Column Chromatography ◽  
Deae Cellulose ◽  
Deae Cellulose Column ◽  
Cellulose Column

scholarly journals Isolation and characterization of a γ-type phosphoinositide-specific phospholipase C (PLC-γ2)

1990 ◽  
Vol 269 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Y Homma ◽  
Y Emori ◽  
F Shibasaki ◽  
K Suzuki ◽  
T Takenawa
Keyword(s):  
Phospholipase C ◽  
Column Chromatography ◽  
Specific Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Deae Cellulose ◽  
Isolation And Characterization ◽  
Delta Type ◽  
Hydrolysis Of ◽  
Cellulose Column

A novel bovine spleen phosphoinositide-specific phospholipase C (PLC) has been identified with respect to immunoreactivity with four independent antibodies against each of the PLC isoenzymes, and purified to near homogeneity by sequential column chromatography. Spleen contains three of the isoenzymes: two different gamma-types [gamma 1 and gamma 2, originally named as PLC-gamma [Rhee, Suh, Ryu & Lee (1989) Science 244, 546-550] and PLC-IV [Emori, Homma, Sorimachi, Kawasaki, Nakanishi, Suzuki & Takenawa (1989) J. Biol. Chem. 264, 21885-21890] respectively] and delta-type of the enzyme, but PLC-gamma 1 is separated from the PLC-gamma 2 pool by the first DEAE-cellulose column chromatography. Subsequently, PLC-delta is dissociated on the third heparin-Sepharose column chromatography. The purified enzyme has a molecular mass of 145 kDa on SDS/polyacrylamide-gel electrophoresis and a specific activity of 12.8 mumol/min per mg with phosphatidylinositol 4,5-bisphosphate as substrate. This enzyme activity is dependent on Ca2+ for hydrolysis of all these phosphoinositides. None of the other phospholipids examined could be its substrate at any concentration of Ca2+. The optimal pH of the enzyme is slightly acidic (pH 5.0-6.5).

Separation of folate binding protein from human serum by DEAE-cellulose column chromatography.

1976 ◽  
Vol 22 (7) ◽  
pp. 1047-1052 ◽  
Author(s):  
A Zettner ◽  
P E Duly
Keyword(s):  
Human Serum ◽  
Column Chromatography ◽  
Binding Protein ◽  
Deae Cellulose ◽  
Chromatographic Behavior ◽  
Binding Property ◽  
Folate Binding Protein ◽  
Diethylaminoethyl Cellulose ◽  
Pteroylglutamic Acid ◽  
Cellulose Column

Abstract On diethylaminoethyl-cellulose column chromatography, the folate binding protein in the serum of 21 patients eluted in the early effluents as a single sharply defined peak. The chromatographic behavior of the folate binder remained unchanged whether or not the serum was, before chromatography, complexed with tritium-labeled pteroylglutamic acid ([3H]PGA), dialyzed, or charcoal-adsorbed. Heating to 100 degrees C for 10 min dissociated the [3H]PGA-binder complex while destroying the folate binding property. The presence or appearance of this folate binder in increased amounts in the serum of patients with various diseases may be related to conditions of increased tissue turnover.

The separation of chlorophylls by paper and cellulose column chromatography

1958 ◽  
Vol 75 (1) ◽  
pp. 56-68 ◽  
Author(s):  
A. Angapindu ◽  
H. Silberman ◽  
P. Tantivatana ◽  
I.R. Kaplan
Keyword(s):  
Column Chromatography ◽  
Cellulose Column

Separation of Anthocyanins by Cellulose Column Chromatography

Nature ◽  
1962 ◽  
Vol 193 (4817) ◽  
pp. 801-802 ◽  
Author(s):  
E. D. GARBER ◽  
W. F. REDDING ◽  
W. CHORNEY
Keyword(s):  
Column Chromatography ◽  
Cellulose Column

scholarly journals The neurotoxins of the sea snake Laticauda schistorhynchus

1983 ◽  
Vol 213 (1) ◽  
pp. 39-41 ◽  
Author(s):  
M L Guinea ◽  
N Tamiya ◽  
H G Cogger
Keyword(s):  
Amino Acid ◽  
Electrophoretic Mobility ◽  
Column Chromatography ◽  
Amino Acid Analysis ◽  
Sea Snake ◽  
Laticauda Semifasciata ◽  
Cellulose Column

Erabutoxins a and b, the major neurotoxins in the venom of the sea snake Laticauda semifasciata, were detected in the venom of Laticauda schistorhynchus. The identity of the toxins was confirmed on the basis of elution position on CM-cellulose column chromatography, disc electrophoretic mobility, amino acid analysis and toxicity measurement.

scholarly journals IMMUNOCHEMICAL STUDIES ON THE GROUP AND TYPE ANTIGENS OF GROUP F STREPTOCOCCI AND THE IDENTIFICATION OF A GROUPLIKE CARBOHYDRATE IN A TYPE II STRAIN WITH AN UNDESIGNATED GROUP ANTIGEN

1967 ◽  
Vol 125 (6) ◽  
pp. 1075-1089 ◽  
Author(s):  
Marc F. Michel ◽  
Richard M. Krause
Keyword(s):  
Cell Wall ◽  
Column Chromatography ◽  
Type Ii ◽  
Cellular Components ◽  
Cellulose Column

Two antigens, the group-specific carbohydrate and the Type II carbohydrate, have been isolated by cellulose column chromatography from a formamide extract of a Group F streptococcus. Chemical and immunologic analyses indicate that both antigens are free of other cellular components. Both antigens are components of the cell wall although the Type II antigen is probably more superficial than the group antigen. The Type II antigen is composed of rhamnose, glucose, galactose, and galactosamine. The Group F antigen is composed of rhamnose, glucose, galactosamine and a small percentage of glucosamine. A grouplike carbohydrate and the Type II carbohydrate have been isolated from a streptotoccal strain which lacks a serologically detectable streptococcal group antigen. This grouplike carbohydrate, which does not cross-react immunologically with Group F serum, is composed of rhamnose, galactose, and glucosamine. No chemical or immunological differences were observed between the Type II antigen isolated from the Group F strain and the Type II antigen isolated from the nongroupable strain.

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