scholarly journals GC-MS ANALYSIS OF BIOACTIVE COMPOUNDS IN ETHANOL LEAVES EXTRACT OF SPHENOCENTRUM JOLLYANUM AND THEIR BIOLOGICAL ACTIVITIES.

2022 ◽  
Vol 06 (01) ◽  
Author(s):  
Emmanuel Uka1,
Keyword(s):  
Molecular Weight ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Octadecenoic Acid ◽  
Acid Ethyl Ester ◽  
Molecular Formula ◽  
Hexadecanoic Acid ◽  
Total Extract ◽  
Medicinal Uses ◽  
Ms Analysis

Sphenocentrum jollyanum is a plant genus of the family Menispermaceae. It has high medicinal importance as it is used traditionally to treat various diseases such as jaundice, breast engorgement related to the menstrual cycle, tumour, fibroids and improve the health of people. The present investigation was carried out to analyze the bioactive compounds present in ethanol crude extract of Sphenocentrum jollyanum leaves using GC-MS analysis. GC-MS analysis of ethanol extract Sphenocentrum jollyanum was done using a 7890A GC system (Agilent Technologies), coupled with 5977B MSD (Agilent Technologies) while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. A total of 45 bioactive compounds representing 99.98% of the total extract based on the retention time, peak area, molecular formula, molecular weight, and biological activities were identified by GC-MS which ranges from high molecular weight to low molecular weight compounds. The major compounds identified with their peak area percentages were 2,4-Di-tertbutylphenol, (21.05%), Z-8-Methyl-9-tetradecenoic acid (19.12), Hexadecanoic acid, ethyl ester (7.86%), Diisooctyl phthalate (7.13%), Phytol, Oleic Acid (7.03), 6,9,12- Octadecatrien-1-ol (6.65%), 3-Eicosene, (E)-(4.63%), 2-Methyl-Z, Z-3,13-octadecadienol (4.24%), n- Hexadecanoic acid (4.09%), trans-13-Octadecenoic acid (3.81%), Cyclohexene, 4-(4-ethylcyclohexyl) -1- pentyl- (3.74%), Dibutyl phthalate (3.20%), and 9-Oxabicyclo (6.1.0) nonane, cis-(3.18%). The presence of these major phytoconstituents in the leaf extract provides various biological activities including antifungal, antibacterial, antioxidant, anti-inflammatory, and anti-tumour which supports the ethno-medicinal uses of the plant in curing diseases. We recommend

scholarly journals GC-MS Analysis of the Rauwolfia vomitoria Ethanol Extracts

2021 ◽  
pp. 34-45
Author(s):  
I. I. Asoro ◽  
O. A. T. Ebuehi ◽  
M. N. Igwo-Ezikpe
Keyword(s):  
Ethyl Ester ◽  
Bioactive Compounds ◽  
Vaccenic Acid ◽  
Octadecenoic Acid ◽  
Acid Ethyl Ester ◽  
Hexadecanoic Acid ◽  
Beneficial Effects ◽  
Ms Analysis ◽  
Pharmaceutical Industries ◽  
Ethanol Extracts

Bioactive compounds are the frontline potent agents in both nutraceuticals and pharmaceutical industries. The bioactive compounds are gaining much importance for their ability in enhancing resistance to various diseases and to improve the health of people both by traditional and modern ways of administrations. R. vomitoria is one of the medicinal plants used traditionally to manage hypertension, diabetes and mental disorder. This present study sought to characterize the bioactive components of R. vomitoria leaf and root ethanol extracts using Gas-Chromatography-Mass Spectrophotometry (GC-MS). The results of the GC-MS analysis provide different peaks indicating the presence of 22 phytochemicals in the plant leaf and 16 phytochemicals in the root. The major bioactive compounds in the leaf were squalene (18.69%), phytol (16.47%), n-hexadecanoic acid (15.68%), 7-tetradecenal, (Z) (12.90%), 9,12,15-octadecatrienoic acid, ethyl ester, (Z,Z)-, (9.56%) and others, while the roots contains; cis-vaccenic acid (32.13%), n-hexadecanoic acid (15.41%), (E)-9-octadecenoic acid ethyl ester (9.83), cyclohexanecarbonitrile 1-(-4- chlorophenyl (9.45%), 8H-azeceno[5,4-b] indol-8-one, 5-ethylidene (7.66%) and other minor compounds. Pharmacological activities of these compounds indicated that the compounds present in the leaf of the plant can be used as a crude drug which could be developed into a novel drug. Some of these compounds have antimicrobial, antioxidant, hepatoprotective, hypocholesterolemic as well as cancer preventive activities amongst others. The findings suggest that there is an indication that both R. vomitoria leaves and roots contain potent bioactive compounds that may be linked to its beneficial effects on health, with the leaf taking the lead. It is therefore recommended as a plant of phytopharmaceutical significance.

BIOACTIVE COMPOUNDS IN ETHANOL EXTRACT OF LENTINUS SQUARROSULUS MONT - A NIGERIAN MEDICINAL MACROFUNGUS

2018 ◽  
Vol 15 (2) ◽  
pp. 42 ◽  
Author(s):  
Morenike Olutunmbi Adeoye-Isijola ◽  
Olufunmiso Olusola Olajuyigbe ◽  
Segun Gbolagade Jonathan ◽  
Roger Murugas Coopoosamy
Keyword(s):  
Ethyl Ester ◽  
Bioactive Compounds ◽  
Octadecadienoic Acid ◽  
Biological Activities ◽  
Ethyl Oleate ◽  
Acid Ethyl Ester ◽  
Ethyl Esters ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Components ◽  
Hexadecanoic Acid

Background: The continuous search for new lead compounds of therapeutic importance has become necessary in the face of treatment failures and multidrug resistance plaguing the world. While many plants and higher fungi are sources of bioactive compounds yet to be fully harnessed, understanding the bioactive components in macrofungus could serve as a lead for investigating its biological activities and medicinal potentials. Materials and Methods: The bioactive compounds in the ethanolic extract of Lentinus Squarrosulus, an edible Nigerian macrofungus, were investigated by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Results: There were nine bioactive compounds in this edible macrofungus. Of these compounds, 9,12-Octadecanoic acid ethyl ester (37.39%; RT:39.815) was the highest in quantity, followed by Hexadecanoic acid ethyl ester (14.49%; RT:36.550). Other fatty acids, their ethyl esters and other compounds identified included 2-Butenethioic acid,3-(ethylthio)-S-(1-methylethyl) ester (4.51%; RT:15.866), n-Hexadecanoic acid (4.74%; RT:36.034), 9,12-Octadecadienoic acid (Z,Z)- (11.88%; RT:39.429), 9,17-Octadecadienal,(Z)- (5.01%; RT:39.500), ethyl oleate (5.27%; RT:39.898), 3a,6-Methano-3aH-indene,2,3,6,7 tetrahydro (4.04%; RT:48.379), and 9,12-Octadecadienoic acid (Z,Z)-,2 hydroxy-1-(hydroxymethyl) ethyl ester (12.68%; RT:48.682). Some of these compounds have antimicrobial, antioxidant, hepatoprotective, hypocholesterolemic as well as cancer preventive activities amongst others. Conclusion: This study showed the bioactive components of therapeutic potentials in L. squarrosulus while creating a platform for screening, isolating and identifying many bioactive components which may be useful in the treatment of the various ailments, disorders and diseases in the nearest future.

scholarly journals Gas Chromatography-Mass Spectrometry (GC-MS) Analysis and Phytochemical Screening of Polyherbal Aqueous Leaves Extract (PALE)

2021 ◽  
pp. 10-18
Author(s):  
M. Idu ◽  
M. O. Aihiokhai ◽  
C. A. Imoni ◽  
C. E. Akokigho ◽  
N. C. Olali
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Octadecadienoic Acid ◽  
Octadecenoic Acid ◽  
Acid Ethyl Ester ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Screening ◽  
Hexadecanoic Acid ◽  
Bioactive Constituents ◽  
Ms Analysis

Background: Polyherbal plant extracts which usually comprise of two or more plant parts often contain a wide array of key phytoactive constituents relevant in attaining greater therapeutic efficacy. The active constituents derived from individual plants are insufficient to provide attractive pharmacological action when compared to a combination of multiple herbs. Objective: To conduct phytochemical screening of polyherbal aqueous leaf extracts                            (PALE) and analysis of compounds present in it by gas chromatography-mass spectrometry (GC-MS). Materials and Methods: The polyherbal extract was prepared from the combined aqueous extracts of leaves of Alchornea cordifolia, Sorghum bicolor and Pennisetum glaucum using ratio 1:1:1 w/v. Phytochemical screening was done via standard analytical methods. The identification and characterization of compounds by GC-MS analysis was performed on gas chromatography system coupled with mass spectrometry. Results: The phytochemical analysis of PALE revealed the presence of phenols, saponins, flavonoids, alkaloids and tannins in varying quantities. GC-MS analysis of the extract depicts the presence of key bioactive compounds. Thirty-two bioactive compounds were identified with various retention time and % peaks. The major compounds identified in terms of % peak area are n-Hexadecanoic acid (6.72), Hexadecanoic acid, ethyl ester (7.28), 9,12-Octadecadienoic acid (16.54) and 9-Octadecenoic acid ethyl ester (12.92). Disulfide, dimethyl (0.04), 2-Methoxy-4-vinylphenol (0.28), 1-Dodecanol (0.85), 10-Phenyldecanoic acid (0.12), 1-Hexadecanol (0.75), Methoxyacetic acid, pentadecyl ester (0.27), 9-Octadecenoic acid (Z)-, phenylmethyl ester (0.16), Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) (1.09), were among the minor compounds identified in the extract. From the study, 9,12-Octadecadienoic acid indicated the highest peak with a retention time of 20.556 minutes and % peak area of 16.54%. Conclusion: The presence of the revealed bioactive constituents in PALE may suggest its nutraceutical, pharmacological and therapeutic relevance. Therefore, in view of the medicinal importance associated with the observed bioactive constituents, further studies on the toxicity level of  the extract is advised subsequently.

scholarly journals Triterpene Esters and Biological Activities from Edible Fruits ofManilkara subsericea(Mart.) Dubard, Sapotaceae

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Caio P. Fernandes ◽  
Arthur L. Corrêa ◽  
Jonathas F. R. Lobo ◽  
Otávio P. Caramel ◽  
Fernanda B. de Almeida ◽  
...  
Keyword(s):  
Ethyl Ester ◽  
Biological Activities ◽  
Octadecenoic Acid ◽  
Acid Ethyl Ester ◽  
Vero Cells ◽  
Octadecanoic Acid ◽  
Hexadecanoic Acid ◽  
Biological Potential ◽  
First Time ◽  
Triterpene Esters

Manilkara subsericea(Mart.) Dubard (Sapotaceae) is popularly known in Brazil as “guracica.” Studies withManilkaraspp indicated the presence of triterpenes, saponins, and flavonoids. Several activities have been attributed toManilkaraspp such as antimicrobial, antiparasitic and antitumoral, which indicates the great biological potential of this genus. In all, 87.19% of the hexanic extract from fruits relative composition were evaluated, in which 72.81% were beta- and alpha-amyrin esters, suggesting that they may be chemical markers forM. subsericea. Hexadecanoic acid, hexadecanoic acid ethyl ester, (E)-9-octadecenoic acid ethyl ester, and octadecanoic acid ethyl ester were also identified. Ethanolic crude extracts from leaves, stems, and hexanic extract from fruits exhibited antimicrobial activity againstStaphylococcus aureusATCC25923. These extracts had highIC50values against Vero cells, demonstrating weak cytotoxicity. This is the first time, to our knowledge, that beta- and alpha-amyrin caproates and caprylates are described forManilkara subsericea.

scholarly journals Gas chromatography-mass- spectroscopy analysis of bioactive compounds from Streptomyces spp. isolated from Tigris river sediments in Baghdad city

2020 ◽  
Vol 14 (1) ◽  
pp. 63-71
Author(s):  
Talib S. Al-rubaye ◽  
Mohsen H. Risan ◽  
Dalal Al-rubaye
Keyword(s):  
Molecular Weight ◽  
Bioactive Compounds ◽  
Mass Spectroscopy ◽  
River Sediments ◽  
Antibacterial Activities ◽  
Hexanoic Acid ◽  
Molecular Formula ◽  
Streptomyces Spp ◽  
Secondary Screening ◽  
Amine Compounds

Background:The Streptomyces are considered the most important bacterial source for bioactive compounds production including natural antibiotics. Objective: This study focused on analysis of these products to characterize the most active substances which may contain new antibiotics. Materials and methods: Samples with the highest antibacterial activities (21, M5, N- and D-) were chosen from a previous study after secondary screening for the intracellular (biomass) extract which showed more antagonism efficiency than that observed in extracellular crude extract.Gas chromatography – mass spectroscopy (GC-MS) was preformed to detect the structure of the compounds in intracellular crude extracts in these isolates. Results: The GC-MS analysis showed a total of 49 peaks observed in 4 isolates, isolate M5=14 peaks, isolate D=11peaks, isolate N= 20 peaks and isolate 21= 4peaks. Isolate D-, which showed the highest zone of inhibition in secondary screening than that in other isolates, is associated with the most prevalence active compounds like the Decane derivatives, in addition to Triadimenol; Azetidine, 1-(1,1-dimethylethyl)-3-methyl; Hexanoic acid, 2-ethyl-, 2-ethylhexyl ester and 3,3,7,7-Tetramethyl-1,5-diazabicyclo(3.3.0)octane. While isolate 21, has less peaks in comparing with the other samples, with great occurrence in components: 1-Dimethylaminohexane with molecular formula C8H19N and molecular weight 129 and Propamocarb with molecular formula C9H20N2O2 and molecular weight 188, in addition to many volatile organic compounds. The greatest components of isolate M5 were Triadimenol and 3,3,7,7-Tetramethyl-1,5-diazabicyclo(3.3.0)octane, in addition to the presence of Decane derivatives; amine compounds and Vitamin E. Isolate N- showed a great occurrence with components Triadimenol and Azetidine, 1-(1,1-dimethylethyl)-3-methyl-with a molecular formula C8H17N and with a molecular weight 127; also the presence of an important component Hexanoic acid, 2-methyl- with the molecular formula C7H14O2 and with molecular weight 103 which has been considered as an essential component of muramycin antibiotic; compounds which contain  Benzene ring. Conclusion: The most prominent compounds detected in the selected isolates by using GC-MS technique were Decane derivatives and Triadimenol.

scholarly journals GAS CHROMATOGRAPHY–MASS SPECTROMETRY ANALYSIS OF METHANOL EXTRACT OF ZANTHOXYLUM RHETSA DC. FRUITS

2018 ◽  
Vol 11 (12) ◽  
pp. 247
Author(s):  
Kuladip Gurav ◽  
Varsha Jadhav (rathod)
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Acid Methyl Ester ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Hexadecanoic Acid ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry

Objective: The aim was to investigate important bioactive compounds, biological activities, and medicinal importance of Zanthoxylum rhetsa fruits.Methods: The present work was carried out by gas chromatography–mass spectrometry (GC-MS) method for the identification of different compounds.Result: The methanolic extract of fruits showed 32 chemical compounds which are identified through GC-MS analysis. Among them, some of the compound names and percentage values are as follows: 2-propanone, 1,3-dihydroxy (48.9%), 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl (33.7%), 2-furancarboxaldehyde, 5-[hydroxymethyl] (50.2%), 1-Heptatriacontanol (34.4%), 9,12-octadecadienoic acid (zz)- (48.6%), cholestan- 3-ol,2-methylene, [3β,5α] (75.0%), 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (90.8), 2-furancarboxaldehyde, 5-[hydroxymethyl] (92.0%), hydroquinone (64.9%), n-hexadecanoic acid (37.5%), octadecanoic acid (28.2%), 9,12,15, octadecatrienoic acid, 2-[(trimethylsiyl]oxy]-1- [(trimethylsiyl]oxy]methyl] ethyl ester, [zzz] (22.6%), 9-hexadecanoic acid (10.3%), digitoxin (18.8%), 8,11,14-eicosatrienoic acid methyl ester, [zzz] (25.5%), and oleic acid (16.5%). Most of the identified compounds in the crude methanolic extracts exhibit some bioactivities, namely anticancer, anti-inflammatory, antimicrobial, hepatoprotective, antioxidant, hypocholesterolemic, nematicide, pesticide, anti-androgenic flavor, hemolytic, 5-alpha-reductase inhibitor, insectifuge, antiarthritic, anti-coronary, cardiovascular, anti-breast cancer, aromatic, and insectifuge. On the basis of the above investigation, the fruits can be recommended as a treasure of bioactive compounds and it plays a promising role in herbal medicine.Conclusion: The present study reveals that fruits of Z. rhetsa contain various bioactive compounds. Digitoxin is recorded in the ripened fruit of Z. rhetsa and it shows the anticancerous and cardiac arrest properties. Hence, in future, this plant will play a promising role in curing cancer.

scholarly journals Phytochemical Profiling of Leaf, Stem, and Tuber Parts of Solena amplexicaulis (Lam.) Gandhi Using GC-MS

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Karthika Krishnamoorthy ◽  
Paulsamy Subramaniam
Keyword(s):  
Molecular Weight ◽  
Bioactive Compounds ◽  
Plant Extracts ◽  
Mass Spectra ◽  
Molecular Formula ◽  
Good Source ◽  
Methanolic Extracts ◽  
Solena Amplexicaulis ◽  
Structure Category ◽  
Molecular Weight Peak

Objective. To explore the possible bioactive compounds in the methanolic extracts of leaf, stem, and tuber parts of the medicinal climber, Solena amplexicaulis, using GC-MS. Methods. GC-MS analysis of the plant extracts were performed by using GC-MS-5975C [Agilent] and mass spectra of the compounds found in the extract was matched with the data in the library of National Institute of Standards and Technology (NIST). Results. Thirty-five compounds were determined to be present in the parts studied. The active principles with their retention time, molecular formula, molecular weight, peak area, structure, category of the compounds, and activities were predicted. The most prevailing compounds were phytol (38.24%) in leaf, 4-(4-ethoxyphenyl) but-3-en-2-one (56.90%) in stem, and 9,17-octadecadienal, (Z)- (21.77%) in tuber. Conclusion. This study revealed that the species S. amplexicaulis is a good source of many bioactive compounds like terpenes, triazines, esters, alkanes, alcohols, hydrocarbons, aldehydes, amides, and so forth. That justifies the traditional usage of this species.

scholarly journals Evaluation of Antibacterial Activity and GCMS Analysis of Zanthoxylum Ovalifolium Fruit Extracts

2021 ◽  
pp. 7-17
Author(s):  
P. Pavani ◽  
Raja Naika
Keyword(s):  
Bioactive Compounds ◽  
Octadecenoic Acid ◽  
Ethyl Oleate ◽  
Herbal Medicines ◽  
Vital Role ◽  
Diffusion Method ◽  
Molecular Formula ◽  
Fruit Extract ◽  
E Coli ◽  
Fruit Extracts

Herbal medicines have played a vital role in the evolution of humanity and civilization. The fruits of the plant Zanthoxylum were collected from the study field, washed, dried in the shade and powdered. Soxhlation method was used to make different solvent extract of fruit. The different fruit extracts were examined against P. aeruginosa, P. syringe, S. aureus, S. typhi, K. pneumonia and E. coli by agar well diffusion method and for the detection of biochemical components present in the Zanthoxylum fruit, methanolic fruit extract was analyzed using GC-MS. Results showed that, the methanolic extract of fruit is found to be exhibit activity against P. aeruginosa, P. syringe, S. aureus, S. typhi, K. pneumonia and E. coli when compared to the hexane and ethyl acetate. The discovery of 46 bioactive compounds in methanolic fruit extract was verified by their molecular formula, molecular weight, and area peak percentage. In which 6 bioactive compounds such as 6-octadecanoic acid, Pentadecanoic acid, Ethyl oleate, 9-octadecenoic acid (Z)-, methyl ester, Glycerin and 1, 2-dimethoxy-4-(2-methoxyethenyl) benzene compounds showed highest peak area percentage of 26.14, 13.61, 8.56, 6.12 and 6.08% respectively. The compounds found in this research may be responsible for some of the medicinal action of the Z. ovalifolium fruit and may be useful in the development of new medicines.

scholarly journals Gas chromatography-mass spectrometry chemical profile investigation and biological activities of ethylacetate fraction of Baobab (Adansonia digitata L.) pulp used in the treatment of urinary tract infections

2022 ◽  
Vol 6 (1) ◽  
Author(s):  
Kehinde O. Fagbemi ◽  
Daniel A. Aina ◽  
Roger M. Coopoosamy ◽  
Olufunmiso O. Olajuyigbe
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Urinary Tract ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Fruit Pulp ◽  
Adansonia Digitata ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Background: As a result of high percentage of women infected with urinary tract infection (UTI) annually, many rural dwellers use Adansonia digitata fruit pulp as herbal cure. Adansonia digitata L. (Malvaceae) is one of the great trees idolised in Africa because of its therapeutic properties.Aim: This study aimed at identifying chemical compounds in the ethylacetate fraction of the baobab fruit pulp and indicated their biological activities to justify its use for the folkloric treatment of UTI.Methods: The crude extract from Baobab fruit pulp was partitioned and ethylacetate extract was used for assay. Gas chromatography-mass spectrometry (GC–MS) analysis was performed to identify the bioactive compounds in the ethylacetate fraction, antioxidant activity was evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays whilst the total phenolic and flavonoid contents were determined by already adopted protocols. The antibacterial properties were tested against some urinary tract pathogens using macro broth dilution method.Results: A total of 36 bioactive compounds were identified by GC–MS analysis, most of them have been reported as antibacterial, antioxidant, antidiabetic, antiasthma, antifungal, anti-inflammatory and anti-cancer agents. The extract exhibited highest activity against Pseudomonas aeruginosa American Type Culture Collection (ATCC) 19582 (1.22 mg/mL) whilst Escherichia coli ATCC 8739 (˃ 8.6 mg/mL) displayed high level of resistance amongst the five bacterial isolates used in this study.Conclusion: These findings indicated that the extract contained bioactive compounds of therapeutic importance with significant antioxidant and antibacterial potentials and justify the folkloric use of this fruit in the treatment of UTIs by many African dwellers.

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