Effects of logging residues on surface soil biochemical properties and enzymatic activity

2014 ◽  
Vol 34 (7) ◽  
Author(s):  
吴波波 WU Bobo ◽  
郭剑芬 GUO Jianfen ◽  
吴君君 WU Junjun ◽  
任卫岭 REN Weiling ◽  
刘小飞 LIU Xiaofei ◽  
...  
PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0144115 ◽  
Author(s):  
Guopeng Liang ◽  
Albert A. Houssou ◽  
Huijun Wu ◽  
Dianxiong Cai ◽  
Xueping Wu ◽  
...  

2009 ◽  
Vol 105 (1) ◽  
pp. 55-62 ◽  
Author(s):  
E. Madejón ◽  
J.M. Murillo ◽  
F. Moreno ◽  
M.V. López ◽  
J.L. Arrue ◽  
...  

Author(s):  
ABEER A. EL-HADI ◽  
HANAN MOSTAFA AHMED ◽  
RANIA A. ZAKI ◽  
AMIRA MOHAMED MOHSEN

Objective: L-asparaginase (L-asp) is a vital enzyme used as a therapeutic agent in combination with other drugs in the treatment of acute lymphoma, melanosarcoma and lymphocytic leukemia. Immobilization of enzymes through loading on nanoemulsion (NE) results in some advantages such as enhancing their stability and increasing their resistance to proteases. Aim of the present study is to formulate L-asp loaded nanoemulsion to enhance its efficiency and thermal stability. Methods: Nanoemulsion loaded with L-asp crude extract (specific activity 13.23U/mg protein) was prepared employing oleic acid as oil, tween 20/tween 80 as surfactants and propylene glycol (PG) as co-surfactant. L-asp loaded NE underwent several thermodynamic stability studies and the optimized formulae were further examined for their biochemical properties and thermal stability. Results The developed formulations were spherical in shape and their sizes were in the nanometric dimensions with negatively charged zeta potential values. Upon comparing the enzyme activity of L-asp loaded NE employing tween 20 (F1) or tween80 (F4) at different concentrations, the results revealed that F4 NE showed higher enzymatic activity [323 U/ml] compared to F1 NE [197 U/ml] at the same concentration. The nanosized immobilized L-asp was more stable in the pH range from 8 to 8.5 as compared to free L-asp. The immobilized enzyme preserved about 59.11% of its residual activity at 50 °C; while free L-asp preserved about 33.84%. Conclusion: In the view of these results, NE composed of oleic acid, tween 80 and PG represents a promising dosage form for enhancing the activity and stability of Streptomyces griseoplanus L-asp.


Forests ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 773
Author(s):  
Sara Huerta ◽  
Víctor Fernández-García ◽  
Leonor Calvo ◽  
Elena Marcos

Recent changes in fire regimes, with more frequent, extensive, and severe fires, are modifying soil characteristics. The aim of this study was to evaluate the effect of burn severity on the resistance of some physical, chemical, and biochemical soil properties in three different forest ecosystems affected by a wildfire in the northwest of the Iberian Peninsula. We evaluated burn severity immediately after fire using the Composite Burn Index (CBI) in three different ecosystems: shrublands, heathlands, and oak forests. In the same field plots used to quantify CBI, we took a composite soil sample to analyse physical (mean weight diameter (MWD)), chemical (pH; total C; total organic C (TOC); total inorganic C (TIC); total N; available P; exchangeable cations Na+, K+, Mg2+, and Ca2+; and cation exchange capacity (CEC)), and biochemical (β-glucosidase, urease, and acid phosphatase enzyme activities) properties. The resistance index of each property was then calculated. Based on our results, the values of the soil chemical properties tended to increase immediately after fire. Among them, total C, TOC, and exchangeable Na+ showed higher resistance to change, with less variation concerning pre-fire status. The resistance of chemical properties was higher in the oak forest ecosystem. MWD decreased at high severity in all ecosystems, but soils in shrublands were more resistant. We found a high decrease in soil enzymatic activity with burn severity, with biochemical properties being the least resistant to change. Therefore, the enzymatic activity of soil could be a potential indicator of severity in forest ecosystems recently affected by wildfires.


Author(s):  
Rafał STRACHEL ◽  
Jadwiga WYSZKOWSKA ◽  
Małgorzata BAĆMAGA

The aim of these studies was to determine the influence of excessive zinc doses on the microbiological and enzymatic properties of soil. Also, an evaluation of the possibility to stimulate remediation processes by nitrogen fertilisation of the soil was attempted. Zinc was applied to loamy sand in the amounts of 0, 250, 500, 750, 1000, 1250 mg Zn2+ kg–1 DM soil, while nitrogen in the form of urea in doses of 0, 250, 500 mg N kg–1 DM soil. Soil samples were incubated at a temperature of 25 °C, maintaining a constant humidity equal to 50% of the maximum water capacity. In the 2nd and 20th week of the experiment, the following factors were determined: activity of dehydrogenases and catalase, and number of organotrophic bacteria, copiotrophic bacteria, oligotrophic bacteria, actinomycetes, and fungi. Zinc inhibited the enzymatic activity of the soil, while causing a slight increase in populations of microorganisms. Only fungi reacted unequivocally positively to contamination of the soil with zinc, therefore demonstrating changes in the biodiversity of microorganisms. Nitrogen fertilisation of the soil resulted in stabilization of the environment contaminated with zinc by stimulation of growth of microorganisms resistant to the influence of this metal.


1998 ◽  
Vol 78 (4) ◽  
pp. 581-587 ◽  
Author(s):  
Roger Lalande ◽  
Bernard Gagnon ◽  
Régis R. Simard

Addition of compost from various sources and of different maturity may affect the soil biochemical properties. A field study was conducted to evaluate the effect of different composts, spring-applied alone or in combination with ammonium nitrate (AN), on microbial biomass C (MBC) and alkaline phosphatase activity (APA) in two soils cropped with spring wheat (Triticum aestivum L. 'Messier') in eastern Quebec, Canada. The experiment was conducted in 1994 and 1995 at different sites on a Kamouraska clay (Orthic Humic Gleysol) and a Saint-André sandy loam (Fragic Humo-Ferric Podzol). Treatments included composts at 180 kg N ha−1, composts at 90 kg N ha−1 supplemented with AN, AN at 90 kg N ha−1, and an unfertilized control. Soil MBC and APA were measured 30 d after compost application and at wheat harvest. Additional sampling was made the following spring. Generally, larger MBC and APA values were found at wheat harvest in soils treated with composts alone than with AN alone or unfertilized. These effects were related to soil C content and climatic conditions. Compost type affected soil biochemical properties which could be attributed to the total C supply and material maturation state. Compost addition constitutes an efficient short-term way to promote soil microbial biomass and enzyme activity in cold climates. Key words: Compost, fertilizer, microbial biomass, soil enzyme, wheat


Sign in / Sign up

Export Citation Format

Share Document